Using a reasonable choice in normalizing the timelike Killing vector, we investigate the thermodynamic properties of charged accelerating Anti-de Sitter (AdS) black holes. We find that the expression of the thermodynamic mass in the first law of thermodynamics displays an inextricably intertwining behavior with the charge due to the unusual asymptotic structure of the accelerating black holes. Meanwhile, the thermodynamic length as a potential conjugate to the varying cosmic string tension is introduced and analyzed in detail, and the possible phase behavior of the charged accelerating black holes is also discussed in a standard thermodynamic analysis. Furthermore, we also investigate the properties of holographic heat engines with charged accelerating AdS black holes as the working substance in a benchmarking scheme. We find that the efficiencies of the black hole heat engines can be influenced by both the size of the benchmark circular cycle and the cosmic string tension. More interestingly, the existence of charge may significantly increase the efficiencies of the black hole heat engines and make them be more sensitive to a varying cosmic string tension. A cross-comparison of the efficiencies of different black hole heat engines suggests that the acceleration also increases the efficiency and renders it more sensitive as the charge varies.