The beef flavor was selected to carry out toxicological analysis regarding food intake, body weight, organs weight and blood biochemical indexes in mice. The results show that beef flavor could suppress food intake but promote mice weight compared with the control group. Flavor intake has significant effect on the ratio of liver and lung weight to the whole body weight, but it has no difference in the ratio of heart, spleen and kidney weight. Some biochemical indexes about fat metabolism in blood such as the total protein, total bile acid and alkaline phosphatase are respectively 4 g/L, 0.7μmol/L and 23 IU/L more than the control group. These factors and cholesterol, triglyceride, low density lipoprotein, high-density lipoprotein cholesterol in the mice blood all have extremely significant differences (P<0.01) in different conditions. The results suggest that the beef flavor has significant impact on fat metabolism in mice.