Background: Low birth weight is a leading cause of Neonatal Mortality. Lack of Nutrients and less intake of Nutrients and minerals during pregnancy is associated with Low birth weight of newborn. To Evaluate the Ass Objective: ociation of Maternal serum Total protein and Albumin levels with birth weight of newborn. A Total of 75 Healthy Mothers of Newborns wi Methods: th Low Birth weight (Birth weight <2.5 Kgs.) were selected as cases and 75 Healthy Mothers of Newborns with Normal Birth weight (Birth weight>2.5 Kgs.) were selected as controls. Serum Total proteins and Serum Albumins were measured in all study subjects. Serum Total proteins were done by Biuret Method and Albumins by BCG Dye Method. Birth weight of Newborns were taken by Beam Balance Scale method at the time of Birth. The study found that the mean levels of Results: Serum Total protein and Serum Albumin in cases (Mothers having LBW newborns) were signicantly lower than the Controls (Mothers having NBW newborns). Mean Serum Total protein and Serum Albumin in cases were 4.98±0.57 gm/dL and 2.65±0.29 gm/dL respectively, while in controls 6.90±0.61gm/dL and 4.06±0.63 gm/dL respectively. Birth weight of newborns showed a posit Conclusion: ive, Signicant and strong Correlation with Maternal Serum Total Protein ('r' = 0.985, p<0.0001) and Maternal Serum Albumin ('r' = 0.961, p<0.0001)