Harlequin Duck (Histrionicus histrionicus) is a small sea duck that winters in coastal waters and breeds on fast-movingmountain streams in western North America. Because of its dependency on streams and coastal near-shore habitat with healthy macroinvertebrate populations, population trends of this species can be used as an indication of healthy freshwater and marine ecosystems. From 1995 to 2020 we conducted roadside surveys for Harlequin Ducks on the Bow River in Banff National Park, Alberta. We calculated the population’s trend by modelling maximum annual count, which showed a population decline over the 25 years of 3.3% per year. The trajectory varied over time: a relatively stable population from 1995 to 2005, a steep decline until 2011, then stabilising at a much lower level with a slight rebound in recent years. The predicted number of ducks from our state-space model closely tracked the maximum number of ducks observed in annual counts. During stable or slightly increasing population estimates the male:female (M:F) ratio fluctuated considerably but stayed high (1.4:1 and 1.3:1, respectively), and during the period of steep population decline the M:F ratio was at its lowest (1.1:1). This declining population trend is concerning because it is occurring in a protected area, but it is similar to data from other studies in the Rocky Mountains and at the coastal wintering area, suggesting that causes may not be solely due to issues on the breeding streams.