Present study was carried out to appraise various biochemical and metabolic changes during spot blotch disease development and efficiency of two plant growth promoting rhizobacterial (PGPR) strains Bacillus safensis W10 and Ochrobactrum pseudogrignonense IP8 in promoting resistance against Bipolaris sorokiniana. In PGPR primed plants which were challenge inoculated with B. sorokiniana, disease index reduced markedly, which was about 50% in B. safensis treatment and 40% in O. pseudogrignonense. Application of PGPR decreased stress indicators like hydrogen peroxide and malonaldehyde accumulated in infected plant tissue as a response to oxidative damage promoted by lipid peroxidation under elevated free radical formation. Increased activity of defense enzymes such as, chitinase, β-1,3 glucanase, phenyl alanine ammonia lyase and peroxidase were observed in plants following application with PGPR and challenge inoculated with B. sorokiniana in comparison with untreated healthy control plants. Besides, accumulation of proline and biochemical components such as total soluble protein and total phenol in higher concentration in PGPR treated and pathogen inoculated plants was evident. Analysis of phenols by high performance liquid chromatography revealed higher accumulation of phenols such as ferulic acid, salicylic acid and caffeic acid.