Objectives:Health promotion is the main strategy for encouraging people to adopt a healthy lifestyle and for preventing non-communicable diseases. Medical students, in particular, are expected to have an important role in health promotion in their near future as physicians. The aim of this study was to determine and evaluate all six components of the Health-Promoting Lifestyle Profile and its sociodemographic determinants among medical students.Methods:A cross-sectional descriptive study was conducted in January 2018 in Jeddah, Saudi Arabia. The questionnaire used in this study consisted of two parts: the first part included sociodemographic questions and body mass index measurement and the second part consisted of questions from the Health-Promoting Lifestyle Profile II.Results:The study enrolled 243 medical students, of which 55.1% were male, 39.5% were in their fifth year, and more than half had a monthly family income above 20,000 Saudi Riyal. The mean body mass index of the respondents was 25.1 ± 5.2 (range = 13.7–43.8). The total mean score of the Health-Promoting Lifestyle Profile II was 123.8 ± 19.8 (range = 72–191). Study findings showed that health-promoting profiles differed by gender, particularly with respect to physical activity and interpersonal relationships. Factors were found to be associated with the Health-Promoting Lifestyle Profile II subscales, including income and year of study.Conclusions:University students, and in particular health science students, represent an appropriate area for health promotion interventions to be established.