The utilization of health research in clinical practice is hindered by individual, organizational, and systemic barriers, including insufficient research literacy and unsupportive organizational cultures. Enhancing research literacy, fostering supportive environments, and implementing evidence-based policies are crucial for bridging the gap between research and practice, ultimately improving patient outcomes. This study investigates the factors affecting health research utilization among health professionals in Nigeria, an essential aspect for advancing healthcare systems and patient outcomes. Utilizing a cross-sectional descriptive design, data were collected via an online survey distributed to public health professionals and policymakers. The survey explored demographics, research utilization, dissemination effectiveness, barriers, and recommendations for improvement. Key findings indicate significant demographic disparities, with a higher proportion of younger and non-public health degree holders reporting unused research findings. The study identified major barriers, including inadequate communication, poor dissemination practices, and lack of access to research findings. Despite 87.6% of respondents conducting potentially impactful research, only 13.6% reported actual utilization of their findings. The recommendations for improvement emphasized the role of knowledge brokers, fostering research-attuned and decision-relevant cultures, enhancing communication and interaction between researchers and users, and building capacity among all stakeholders. Additionally, establishing effective dissemination systems, securing funding, and promoting international collaboration were highlighted as critical strategies. The study underscores the necessity of addressing structural, cultural, and communicative barriers to improve the practical application of health research in Nigeria, aligning with global trends and literature advocating for a multifaceted approach to enhance health outcomes.