Health Of Dairy Cattle Research Articles

Climate Change Perceptions and Adaptations for Dairy Cattle Farmers in Jordan: Case Study in North East Region -Al-Dhulel Area

"This study was designed to investigate how dairy farmers of AL-Dhulel cooperative Dairy Society (ACDS) perceive climate change, the adaptation strategies adopted by farmers to cope with the impact of climate change and the barriers to the adoption of these strategies. A 92 dairy farmers provided with a questionnaire that was developed to collect the data and covered farmers perception, adaptation strategies, and the barriers facing them towards adopting the strategies. The personal interviews with the farmers were performed during early January, 2020. The data was analyzed using the Statistical Package for Social Sciences (SPSS). The main result obtained from the study that most of dairy farmers were aware of the climate change impact on dairy cattle performance and health. Furthermore, the adaptation strategies that was suggested has limiting factors according to farmers as a result of governmental and agricultural institutions restriction polices. Therefore, recommendations regarding new polices was suggested to facilitate the way of getting benefit from grants and financial support for improving dairy farms and to mitigate the effect of climate change on dairy cattle."

Farm 4.0: innovative smart dairy technologies and their applications as tools for welfare assessment in dairy cattle

Abstract The growing world population has increased the demand for meat and milk products and has led to a rapid growth in the scale of cattle enterprises globally. As a result, livestock production would intensify even more with farmers aiming to maximize output per animal unit and it would lower the stockperson per animal ratio. This will result in less time available to monitor and manage individual animals properly, jeopardizing animal health and welfare. The recent advancement in technological and engineering tools and materials such as advanced sensors and sensing devices, and data processing and machine learning methods provide effective tools for the dairy industry to control qualitative and quantitative parameters related to dairy cattle health and welfare. This review paper will (a) explain smart dairy technologies, (b) describe on-farm cattle welfare assessment, and (c) discuss sensors applied in automatic cattle welfare assessment.

Clinical, Hematological and Some Serum Biochemical Alterations in Local Cows Affected with Milk Fever in Gogjalee Region, Mosul, Iraq

Milk fever (Hypocalcemia) is a metabolic disorder that has serious economic impacts on health of dairy cattle and production. Current research endeavored to evaluate the clinical, hematological and some biochemical alterations in cows affected with milk fever in the Gogjalee region, Mosul in Iraq. This research was conducted on 30 local breed cows aged (three to seven years) from private farms diagnosed with postparturient paresis according to clinical signs and laboratory confirmations. Ten healthy cows served as control groups.Blood samples were taken from each animal for biochemical and hemogram analysis. Results showed typical clinical signsof milk fever with different stages of the disease. Significant (P<0.05) increasesin the body temperature, respiratory rates and significant (P<0.05) decreases in heart rates in affected animals. Older ages (five to seven years)had considerably higher rate of 73.33%( n=22 ) than three to four-years-old with 26.66%(n=8 ). No significant changes were seen in the blood picture except in the total leukocytic count(TLC). Results revealed significant decrease of Calcium, Inorganic phosphorus, and significant increase of magnesium, haptoglobin(Hp), aspartate amino transferase(AST), alanine amino transferase (ALT), creatine kinase (CK), and no significant changes in total protein levels compared tothecontrol groups. Our study indicates the prevalence of milk fever in local breed cows in Gogjalee region. A certain parameters (Ca, P and Mg), hepatic enzymes (ALT, AST, CK and Hp) are also useful and important in the diagnostic process for detection of milk fever.

The National Animal Health Monitoring System's perspective on respiratory disease in dairy cattle.

In 1991, USDA's National Animal Health Monitoring System (NAHMS) conducted its first dairy study, a baseline assessment that focused on the health and management of heifer calves. During the study, producers ranked respiratory disease among the top two most common health problems affecting dairy calves. Over the last 25 years, U.S. dairy producers have participated in six NAHMS studies, yielding a rich repository of information that has helped identify needs for research, extension, and education in the dairy industry. NAHMS' most recent dairy study, conducted in 2014, provides the latest estimates on dairy cattle health and includes another in-depth look at heifer-calf health. While overall mortality in calves has decreased, bovine respiratory disease (BRD) remains an important cause of morbidity in calves. This raises the concern that BRD mitigation may be at a standstill on dairies. Research and on-farm experience have done much to elucidate the challenges associated with detection and perceived impacts of this complex disease. Continued development and implementation of new methods for monitoring health and detecting disease will provide additional tools to upend stalemating factors associated with BRD control, helping the dairy industry 'turn a corner' on this important disease.

Skeletal health, redox balance and gastrointestinal functionality in dairy cows: connecting bugs and bones.

This research reflection examines the physiological links between redox balance, skeletal health and gastrointestinal functionality in dairy cows. With the increase in demand of animal products caused by the growth in human population, the dairy industry needs to develop and implement innovative strategies which are profitable, sustainable and cow friendly. Redox balance, skeletal heath and gastrointestinal functionality are three key physiological systems that are often seen as independent entities. In this research reflection we intend to stress that the antioxidant system, bone health and the microbiome are intimately intertwined. Antioxidants are crucial for the maintenance of redox homeostasis and optimal immune function. Optimal gastrointestinal functionality is important to maintain animal performance, health and welfare. In particular, the intestinal microbiome is increasingly seen as a driver of health and disease. Vitamin D metabolism is pivotal not only for optimal skeletal health, but in light of all the extra-skeletal effect of vitamin D, it is the foundation for optimal productive life. It makes sense to ask the question 'how are redox balance and the microbiome involved in the modulation of bone health and immune function?' In other words, are bugs and bones connected in dairy cows! The existing data available in the literature suggests that this might be the case. The characterization of the interactions between redox balance, skeletal health and the microbiome, will allow the development of a multisystem biological approach to refine nutritional interventions to improve dairy cattle health, welfare and productive longevity.

Комплексная оценка скота молочного направления продуктивности

Abstract. The purpose of the investigation was a survey of the modern complex index estimation of dairy cattle in the countries of the European Union and the USA and developing of own principal scheme of multipurpose evaluation of animals due to prior received data of economic useful traits. As a result of the research, significant differences were revealed both between the significance of the groups of traits and between the numbers of evaluated indicators in different countries. This shows the diversity of interests of breeders and farmers. The prior analysis of indicators of economic useful traits of Holstein Kholmogorskiy cattle in the farms of the Murmansk region allowed to determine main directions of animal selection and to develop the algorithm of animal evaluation by complex of economic useful trait indices. The developed algorithm of complex evaluation for dairy cattle includes four evaluation blocks – indices of reproductive ability, longevity, and milk production, and, separately, milk yield. We propose to evaluate reproductive abilities by direct indicators and indirect indicators, for example, take into account exterior traits, and predisposed to abortion and female disorders. The evaluation of longevity includes indices of the exterior, accounted also a predisposition to four main groups of disease – udder disease, female disorders, aborts, lame. The milk productivity evaluation includes traits of the milk quality composition, the property of the lactation curve and characteristics of the udder from the exterior and predispose to udder diseases. In total, it is proposed to calculate the multipurpose evaluation of animals by 23 traits, including all aspects of health and productive features of dairy cattle. The scientific novelty of the research is the use of a mixed model affecting various indicators of productivity and longevity for assessing the main economical useful traits in the developed algorithm. The developed assessment system will allow to comprehensively improve the herd taking into account the current state of dairy cattle breeding in the Murmansk region.

Confirmed effects of candidate variants for milk production, udder health, and udder morphology in dairy cattle

BackgroundOver the last years, genome-wide association studies (GWAS) based on imputed whole-genome sequences (WGS) have been used to detect quantitative trait loci (QTL) and highlight candidate genes for important traits. However, in general this approach does not allow to validate the effects of candidate mutations or determine if they are truly causative for the trait(s) in question. To address these questions, we applied a two-step, within-breed GWAS approach on 15 traits (5 linked with milk production, 2 with udder health, and 8 with udder morphology) in Montbéliarde (MON), Normande (NOR), and Holstein (HOL) cattle. We detected the most-promising candidate variants (CV) using imputed WGS of 2515 MON, 2203 NOR, and 6321 HOL bulls, and validated their effects in three younger populations of 23,926 MON, 9400 NOR, and 51,977 HOL cows.ResultsBull sequence-based GWAS detected 84 QTL: 13, 10, and 30 for milk production traits; 3, 0, and 2 for somatic cell score (SCS); and 8, 2 and 16 for udder morphology traits, in MON, NOR, and HOL respectively. Five genomic regions with effects on milk production traits were shared among the three breeds whereas six (2 for production and 4 for udder morphology and health traits) had effects in two breeds. In 80 of these QTL, 855 CV were highlighted based on the significance of their effects and functional annotation. The subsequent GWAS on MON, NOR, and HOL cows validated 8, 9, and 23 QTL for production traits; 0, 0, and 1 for SCS; and 4, 1, and 8 for udder morphology traits, respectively. In 47 of the 54 confirmed QTL, the CV identified in bulls had more significant effects than single nucleotide polymorphisms (SNPs) from the standard 50K chip. The best CV for each validated QTL was located in a gene that was functionally related to production (36 QTL) or udder (9 QTL) traits.ConclusionsUsing this two-step GWAS approach, we identified and validated 54 QTL that included CV mostly located within functional candidate genes and explained up to 6.3% (udder traits) and 37% (production traits) of the genetic variance of economically important dairy traits. These CV are now included in the chip used to evaluate French dairy cattle and can be integrated into routine genomic evaluation.

Evaluating the need for an animal welfare assurance programme in South Tyrolean dairy farming

Animal welfare assurance is of great importance as dairy farming is recently under increasing pressure to meet societal and commercial expectations. Therefore, this study aims to determine the current welfare situation of dairy cattle housed in tie-stalls and free stalls in South Tyrol (Eastern Italian Alps) in order to evaluate the need for establishment of an animal welfare assurance programme. For reasons of research economy, a protocol was used for data collection that would also be applicable for practical use in an animal welfare assurance scheme. Analyses of resource-based and animal-based indicators recorded in 204 farms in North and South Tyrol (1891 dairy cows) reveal some important animal welfare problems mainly related to the provision of resources and the prevalence of skin alterations especially in tie-stall barns, which are still widely spread in mountain areas. Hence, the implementation of an animal welfare assurance scheme is urgently needed to reflect public concerns through regular and standardised monitoring of welfare indicators and continuous encouragement of improvements in dairy cattle welfare towards predefined targets. Concerning tie-stalls, interventions in stall design as well as the selective use of local breeds best adapted to the mountainous conditions appear to be appropriate measures to optimise dairy cattle health and welfare. These findings substantiate the high value of the data that would be collected as part of the assurance programme to gain insights, which could be used in preventive and corrective health plans to improve welfare-friendliness in dairy farming of South Tyrol. Highlights An adverse effect on animal welfare in tie-stalls was pointed out, even though this housing system is still widely spread in the Alpine region. An animal welfare assurance programme for dairy cattle is urgently needed in South Tyrol, where some welfare problems currently exist. Regular and standardised monitoring of indicators encourages improvements in dairy cattle health and welfare.

Association between lameness risk assessment and lameness and foot lesion prevalence on dairy farms in Alberta, Canada

Lameness is a detrimental health and welfare concern of dairy cattle with high prevalence in North American herds. As a practical and farm-specific approach toward its mitigation in Alberta, Canada, a score-based lameness risk assessment questionnaire (RAQ) was developed for veterinarians to conduct with their clients; however, its accuracy in identifying risk for lameness and hoof lesions has not been evaluated. Our objectives were to (1) provide an update on prevalence of lameness and hoof lesions in Alberta freestall herds, (2) validate the RAQ by determining the association between RAQ scores and the prevalence of herd lameness and herd lesions, and (3) determine the association between presence of common hoof lesions and lameness. For the first 2 objectives, 65 Alberta dairy farms were visited between March and October 2018 to collect a completed RAQ, hoof trimming records from their hoof trimmer, and video footage of the entire lactating herd. For the third objective, 10 participant herds were visited twice between November 2013 and June 2014 to take video footage of the entire lactating herd and collect hoof trimming records from their hoof trimmer within 48 h after video footage collection. Median herd-level lameness prevalence was 20% (range = 2-56%), infectious lesion prevalence was 10% (range = 0-49%), and noninfectious lesion prevalence was 15% (range = 2-39%). Of cows that were lame, 93% had a lesion, whereas 21% of cows with a lesion were lame. Cows with an infectious and noninfectious lesion were, respectively, 1.5 and 3.1 times more likely to be lame than cows with no lesion. Total RAQ score and herd lameness prevalence were correlated (r = 0.26) and noninfectious RAQ score and noninfectious lesion prevalence were correlated (r = 0.45), whereas infectious RAQ score and infectious lesion prevalence were not. Cow-level mixed logistic regression models indicated no associations between any of the RAQ scores and presence of lameness or a hoof lesion. Lameness prevalence in Alberta remains high, indicative of a low adoption rate of mitigation strategies by producers. Improvement of the RAQ could allow for better reflection of lameness and lesions on farm and can be achieved through further risk factor analysis within the RAQ, which may result in removal or addition of questions as well as the adjustment of scores based on risk factors' strength of association with lameness and lesions.

A review of vitamin D and its importance to the health of dairy cattle.

This Research Reflection short review will discuss vitamin D metabolism, its role in nutrition, disease prevention, and welfare of dairy cattle, as well as its toxicity. Vitamin D is an important fat-soluble vitamin. However, some researchers regard it as a hormone due to its function in the organism. Its role is not limited just to Ca homoeostasis and bone metabolism but is also associated with immunity. In dairy cattle it is known for preventing milk fever. Cows can acquire vitamin D in many ways for example through feed, parenteral injections or through UVB irradiation from the sun or artificial lighting. The vitamin D in feed can either be plant-/ fungi- based ergocalciferol or animal-based cholecalciferol. There is currently only one registered feed vitamin D supplement for cattle in the European Union and it is cholecalciferol. Animals can also synthesize their own vitamin D when 7-dihydrocholesterol in the skin is irradiated with UVB light resulting in cholecalciferol production. Despite its importance, many cattle are deficient in vitamin D due to inadequate supplementation or insufficient sun exposure. In a study performed at the Veterinary Faculty in Slovenia 12 high producing Holstein Friesian cows at a commercial dairy farm were blood tested for vitamin D status for three succeeding months and all but one were vitamin D insufficient in all testings. The cows were not exposed to direct sunlight and the content of vitamin D3 in feed was <400 IU/kg dry matter, which is less than half of the NRC (2001) recommendation. Deficiency can also occur due to diseases affecting the gastrointestinal tract, such as paratuberculosis, which lower the absorptive capacity of the gut. Vitamin D can be toxic if cows are over-supplemented or consume large quantities of plants like Trisetum flavescens, which contain an active form of vitamin D-calcitriol or its glycosides, that are activated by digestion in the rumen.

Genome-Wide DNA Methylation and Gene Expression Profiles in Cows Subjected to Different Stress Level as Assessed by Cortisol in Milk.

Dairy cattle health, wellbeing and productivity are deeply affected by stress. Its influence on metabolism and immune response is well known, but the underlying epigenetic mechanisms require further investigation. In this study, we compared DNA methylation and gene expression signatures between two dairy cattle populations falling in the high- and low-variant tails of the distribution of milk cortisol concentration (MC), a neuroendocrine marker of stress in dairy cows. Reduced Representation Bisulfite Sequencing was used to obtain a methylation map from blood samples of these animals. The high and low groups exhibited similar amounts of methylated CpGs, while we found differences among non-CpG sites. Significant methylation changes were detected in 248 genes. We also identified significant fold differences in the expression of 324 genes. KEGG and Gene Ontology (GO) analysis showed that genes of both groups act together in several pathways, such as nervous system activity, immune regulatory functions and glucocorticoid metabolism. These preliminary results suggest that, in livestock, cortisol secretion could act as a trigger for epigenetic regulation and that peripheral changes in methylation can provide an insight into central nervous system functions.

Vaginal Microbiota Changes During Estrous Cycle in Dairy Heifers.

The vaginal microbiota plays an important role in the health of dairy cattle, and it could be manipulated for the prevention and treatment of reproduction-related infections. The present study profiles and compares the vaginal microbiota of healthy dairy heifers during the estrous cycle focusing the results in follicular (estrus) and luteal (diestrus) phases using 16S rRNA sequencing of the V3–V4 hypervariable region. Twenty 13–16-months-old virgin dairy heifers from a single farm were included in this study. Vaginal swabs and blood samples were obtained during estrus (6–8 h before artificial insemination) and diestrus (14 days after insemination). Estrus was evaluated by an activity monitoring system and confirmed with plasma progesterone immunoassay. Results showed that the taxonomic composition of the vaginal microbiota was different during the follicular and luteal phases. At the phylum level, the most abundant bacterial phyla were Tenericutes, Firmicutes, and Bacteroidetes which comprised more than 75% of the vaginal microbiota composition. The next more abundant phyla, in order of decreasing abundance, were Proteobacteria, Actinobacteria, Fusobacteria, Epsilonbacteraeota, and Patescibacteria. Together with Tenericutes, Firmicutes, and Bacteroidetes represented more than 96% of the bacterial composition. Ureaplasma, Histophilus, f_Corynebacteriaceae, Porphyromonas, Mycoplasma, Ruminococcaceae UCG-005, were the most abundant genera or families. The results also showed that the vaginal microbiota of dairy heifers was non-lactobacillus dominant. The genus Lactobacillus was always found at a low relative abundance during the estrous cycle being more abundant in the follicular than in the luteal phase. Despite more research is needed to explore the potential use of native vaginal microbiota members as probiotics in dairy heifers, this study represents an important step forward. Understanding how the microbiota behaves in healthy heifers will help to identify vaginal dysbiosis related to disease.

Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle.

Selenium is an important element in nutrition, showing great potential in the udder health of dairy cattle and in the control of subclinical mastitis. However, there are few studies on selenium and subclinical mastitis in cows, and the correlation is not clear. A trial was designed to investigate the association between serum selenium levels and the immune and antioxidant capacity of dairy cattle with subclinical mastitis. Fifty cattle in early lactation with similar background information were selected randomly from an intensive dairy farm. Blood samples were collected for the detection of serum selenium levels by ICP-optic emission spectrometer. The cattle were divided into a low-selenium group (< 0.05mg/L) and three normal selenium groups with different serum selenium levels (0.05-0.08mg/L). The somatic cell count, immune indexes, and other indexes in the milk of each group were detected. The somatic cell count was found to be negatively correlated with serum selenium level. GSH-Px had a low positive correlation and IL-6 had a low negative correlation with serum selenium level. With a serum glutathione peroxidase < 148U/L and IL-6 > 451pg/mL, the risk of subclinical mastitis in dairy cattle increased.

Structural equation modeling for investigating multi-trait genetic architecture of udder health in dairy cattle

Mastitis is one of the most prevalent and costly diseases in dairy cattle. It results in changes in milk composition and quality which are indicators of udder inflammation in absence of clinical signs. We applied structural equation modeling (SEM) - GWAS aiming to explore interrelated dependency relationships among phenotypes related to udder health, including milk yield (MY), somatic cell score (SCS), lactose (%, LACT), pH and non-casein N (NCN, % of total milk N), in a cohort of 1,158 Brown Swiss cows. The phenotypic network inferred via the Hill-Climbing algorithm was used to estimate SEM parameters. Integration of multi-trait models-GWAS and SEM-GWAS identified six significant SNPs for SCS, and quantified the contribution of MY and LACT acting as mediator traits to total SNP effects. Functional analyses revealed that overrepresented pathways were often shared among traits and were consistent with biological knowledge (e.g., membrane transport activity for pH and MY or Wnt signaling for SCS and NCN). In summary, SEM-GWAS offered new insights on the relationships among udder health phenotypes and on the path of SNP effects, providing useful information for genetic improvement and management strategies in dairy cattle.

Improvement of claw health of cattle in Switzerland

The modern technique of cattle hoof care was founded by E. Toussaint Raven in 1977. Environmental risk factors on cattle claws altered in the past 43 years. The change from free ranging to indoor housing, the intensified feeding and the breeding towards traits of high performance have significantly increased the mechanical and chemical stress on the claws. In modern free-stalls, dairy cows are required to walk on hard flooring to feed, drink and get milked. Good hoof health is a basic requirement for cattle welfare. Professional and regular hoof trimming is still considered the most effective measure to promote hoof health in dairy cattle. In order to meet today's requirements and to promote claw health, the Swiss Hoof Trimmers Association (SKV), in collaboration with the Vetsuisse faculties, Universities of Berne and Zurich, and the Bovine Health Service (RGD, Bern) developed and described the Swiss technique of functional claw trimming. The aim was to establish a consistent method, which takes into account the size and bodyweight of the modern cow, the anatomical and physiological characteristics of their claws and includes adaptations counteracting very relevant diseases such as digital dermatitis. The result is a workflow described and illustrated with coloured pictures and consisting of five individual steps based on the technique of E. Toussaint Raven, Additionally, the upcoming Swiss national resource project on long-term improvement of claw health is presented in some detail. The key point of this project is the electronic documentation of clinical findings by the trained professional claw trimmers. This data will later (i) be used to assess the foot health of Swiss cows, (ii) allow to determine the prevalence of foot diseases of cattle in Switzerland and (iii) to monitor the effect of the implementation of foot health concepts. The aim of this work is to combine the findings from science and the practical experience of hoof trimmers in one method, to standardize the applied hoof care in Switzerland and to adapt it to today's hoof health requirements.

Effect of abortion on milk production, health, and reproductive performance of Holstein dairy cattle

The objective of this study was to identify the effect of abortion on performance of Iranian Holstein cows that calved between 2005–2014. Abortion records were categorized in two groups: 1) new abortion (NLA), and 2) rebreeding abortion (RA). Production data (305-d milk, fat, and protein yields) were analyzed using PROC MIXED, number of inseminations per lactation period (INS), postpartum health disorders (PPHD), and culling records using PROC GLIMMIX, and calving to conception records using PROC PHREG of SAS software (SAS Institute, 2013). For each separate model, main herd-cow factors and all two-way significant interactions were considered in the analyses with results being reported for each calving status by lactation period. In total, 12.5 % of lactation periods were associated with at least one case of abortion, while 1.9 % of the lactation periods commenced with an abortion. Based on results from the present study, milk production decreased for cows having abortion classified as NLA or RA. Overall, when there was a RA, there was an increase in median days non-pregnant (DNP) by 132 d and INS by 1.6, whereas cows with NLA had median DNP of 15 d less and an INS of 0.17 less than cows with normal parturitions. Compared with cows with normal parturitions, cows with a NLA were 1.24, 1.88, and 2.41 times more likely to have PPHD, and be culled from the herd for involuntary reasons and reasons related to reproduction, respectively. The results of this study indicate abortions were associated with cow health and milk production and reproductive performance, indicating losses due to abortion are not only a direct result of the loss of a pregnancy.

Influence of saltwater intake on the health of dairy cattle

Water is an element extremely important, essential for life, and necessary to maintain some factors such as intracellular pressure, assist digestive processes, carry nutrients, eliminate toxins through urine and allow the thermal balance of living things. However, it is a scarce element as the national water resources policy in Brazil advocates. In recent years water has become one of the biggest global problems due to lack and quality. The objective of this study was to evaluate the influence of saline intake on the health of dairy cattle. The actions consisted of periodic visits to a rural property located in the city of Pesqueira, Agreste Pernambucano - Brazil, whose main economic activity is milk production and cheese making. The herd has milk aptitude and consists of 182 animals. Samples of desalination tailings water that were supplied for quench the animals were collected. Blood samples from the cattle were collected and sent for hematological exams. The results showed alterations of some physicochemical parameters of water and high serum levels of chloride and sodium in cattle. The continuous intake of wastewater from the desalination process for the quench of dairy cattle alters the serological and physiological patterns of these animals. Further studies must be conducted on dairy products regarding their quality and health impact.

A Review of the Impact of Mycotoxins on Dairy Cattle Health: Challenges for Food Safety and Dairy Production in Sub-Saharan Africa.

Mycotoxins are secondary metabolites of fungi that contaminate food and feed and have a significant negative impact on human and animal health and productivity. The tropical condition in Sub-Saharan Africa (SSA) together with poor storage of feed promotes fungal growth and subsequent mycotoxin production. Aflatoxins (AF) produced by Aspergillus species, fumonisins (FUM), zearalenone (ZEN), T-2 toxin (T-2), and deoxynivalenol (DON) produced by Fusarium species, and ochratoxin A (OTA) produced by Penicillium and Aspergillus species are well-known mycotoxins of agricultural importance. Consumption of feed contaminated with these toxins may cause mycotoxicoses in animals, characterized by a range of clinical signs depending on the toxin, and losses in the animal industry. In SSA, contamination of dairy feed with mycotoxins has been frequently reported, which poses a serious constraint to animal health and productivity, and is also a hazard to human health since some mycotoxins and their metabolites are excreted in milk, especially aflatoxin M1. This review describes the major mycotoxins, their occurrence, and impact in dairy cattle diets in SSA highlighting the problems related to animal health, productivity, and food safety and the up-to-date post-harvest mitigation strategies for the prevention and reduction of contamination of dairy feed.

Low level of genetic diversity and high occurrence of vector-borne protozoa in water buffaloes in Thailand based on 18S ribosomal RNA and mitochondrial cytochrome b genes

Vector-borne pathogens (VBPs) pose a great risk to ruminant production through significant economic losses. Several previous studies in Thailand have mainly been focused on the health of dairy and beef cattle. Water buffaloes are one of the important ruminants in the country, but studies on their infection with VBPs remains limited. We conducted a molecular survey on blood samples from 456 buffaloes obtained from eight provinces across different geographical locations of Thailand. The PCR diagnostics indicated that 116 (25.4%) and 59 (12.9%) of these 456 samples were positive for piroplasm and Plasmodium spp., respectively, and were found in six and all eight regions, respectively, across Thailand. Co-infections of piroplasm and Plasmodium spp. were observed in 24 cases (5.26%). Babesia spp. was not detected in any of the 12 sequenced piroplasm-positive samples in the present study. Genetic comparisons and phylogenetic analyses of within and between parasite populations, based on the 18S ribosomal (r)RNA and cytochrome b (cytb) genes for T. orientalis and P. bubalis, respectively, revealed that T. orientalis shared a high similarity within its population and could be divided into four distinct haplotypes. Haplotypes 1 and 4 were placed in the same clade with the samples previously isolated from cattle in Korea, Japan, Australia, and the USA. Haplotypes 2, and 3 were novel and were placed in a separate clade not shared with the other isolates. We also confirmed our previous investigation that at least three cytb haplotypes of P. bubalis were distributed in the country with a relatively high degree of genetic polymorphisms within its population (based on cytb sequences). Type II P. bubalis was phylogenetically closely related to P. caprae in goats in Zambia and Thailand. This study improves our current understanding on the distribution, intra- and inter-population genetic diversity, and genetic relationship of piroplasms and Plasmodium spp. in water buffaloes.

Prenatal programming of later performance in dairy cattle

Prenatal programming refers to the fact that insults during pre- and early postnatal life can have long-term consequences on the health and performance. In diary cattle, physiological conditions, such as maternal body growth, milk yield and parity, and environmental conditions during gestation can create a suboptimal environment for the developing fetus. As a consequence, adaptations of the placental and newborn phenotype take place. In addition, potential long-term effects of prenatal programming influence body growth, fertility, milk yield and longevity in dairy cows. These results suggest that the current management systems may pose a risk for the long-term health and performance of dairy cattle. Hence, in management practices, all pre- and postnatal aspects should carefully be considered in order to raise healthier and more productive dairy cows.