Background: Osteoporosis, a significant public health concern, necessitates informative health interventions due to its largely modifiable risk factors. Non-pharmacological approaches play a vital role in managing this condition. This survey aimed to assess the impact of an educational intervention on osteoporosis knowledge among nursing professionals. Objective: The primary objective was to evaluate the effectiveness of a health information brochure as an educational tool in enhancing osteoporosis awareness among nurses. Methods: Employing a comparative cross-sectional design, the study systematically sampled 278 nurses using the Roasoft calculator. Data were collected through a pre- and post-intervention osteoporosis quiz, with a health information brochure utilized for the intervention. Results: The study achieved a 100% response rate. Pre-intervention, 70.9% of nurses correctly identified that osteoporosis affects both genders, which increased to 82.4% post-intervention. Statistical analysis revealed a weak correlation in bone health knowledge (r=0.35, p<0.001), a strong association in understanding risk factors (r=0.70, p<0.001), and treatment and prevention (r=0.89, p<0.001). Significant differences in knowledge improvement were confirmed through paired t-test statistics. Conclusions: The survey demonstrated a statistically significant improvement in osteoporosis knowledge following the educational intervention. The results advocate for the integration of enhanced osteoporosis-related education in nursing curricula and continuing education programs, addressing the identified gap in responsiveness and knowledge as a barrier to optimal patient care.