Background: According to the World Health Organization, annually there are around 50.0-100.0 million cases of dengue fever worldwide, with 0.25-0.50 million of them being cases of dengue hemorrhagic fever (DHF) which causes around 24,000 death. Objective: The study was directed at evaluating the knowledge and attitudes of mothers regarding dengue prevention in Punge Blang Cut, Jaya Baru. Method: A cross-sectional design was used in the study conducted in April 2019. Around 176 heads of families in the village were sampled. Data was collected through a questionnaire with the Chi-Square test at a significance of 0.05. Results: The majority of respondents had sufficient knowledge (46.6%), their mothers had a positive attitude towards dengue prevention (73.3%), and 52.3% of respondents took sufficient preventive measures. Conclusion: The majority of mothers have sufficient knowledge and have a positive attitude towards preventing dengue fever. It is recommended to increase public knowledge and awareness regarding the handling and prevention of dengue fever through appropriate medical and traditional methods.