Head Blood Vessels Research Articles

Nomogram model for predicting early onset of chronic kidney disease using color Doppler region of interest technique.

The risk factors of chronic kidney disease were analyzed by using the region of interest quantitative technology of color Doppler combined with QLab software, and a Nomogram was established to conduct an individualized assessment of patients with chronic kidney disease. A total of 500 patients with chronic kidney disease diagnosed in our hospital from June 2019 to March 2021 were selected as the chronic kidney disease group, and 300 healthy patients during the same period were selected as the control group. Univariate analysis was performed on the test indexes and the vascularity index, flow index, and vascularization flow index measured by the color doppler region of interest quantitative technique. The above meaningful indicators were included in the Logistics regression analysis to obtain the independent risk factors of early chronic kidney disease. The independent risk factors were imported into R software to draw a Nomogram model for predicting early chronic kidney disease and evaluate the model. Single factor analysis results suggest age, hypertension, diabetes, hyperlipidemia, disease of heart head blood-vessel, body mass index, vascularity index, flow index, and vascularization flow index, fasting blood sugar, triglyceride, total cholesterol, urea nitrogen, creatinine, uric acid, glomerular filtration rate differences statistically significant (P < 0.05). Logistics regression analysis showed that hypertension, diabetes, flow index, and vascularization flow index, urea nitrogen, and albumin were independent risk factors for the early occurrence of chronic kidney disease. The C-index of this Nomogram using independent risk factors is 0.896 (95%CI 0.862-0.930), which indicates that the Nomogram has good discriminant power. The receiver operating curve of the histograph was area under the curve (AUC) 0.884 (95%CI 0.860-0.908). The receiver operator characteristic curve (ROC) of urea nitrogen, albumin, flow index, and vascularization flow index were evaluated. The results indicated that the best cutoff value of urea nitrogen was 5.9mmol/L, flow index was 14.67, vascularization flow index was 4.6, and albumin was 40.26g/L. In the prediction of chronic kidney disease I-II stage, the quantitative technique of color Doppler region of interest has certain diagnostic value. The model established in this study has good discriminative power and can be applied to clinical practice, giving certain indicative significance.

Developmental toxicity of TCBPA on the nervous and cardiovascular systems of zebrafish (Danio rerio): A combination of transcriptomic and metabolomics

Tetrachlorobisphenol A (TCBPA), a widely used halogenated flame retardant, is frequently detected in environmental compartments and human samples. However, unknown developmental toxicity and mechanisms limit the entire understanding of its effects. In this study, zebrafish (Danio rerio) embryos were exposed to various concentrations of TCBPA while a combination of transcriptomics, behavioral and biochemical analyzes as well as metabolomics were applied to decipher its toxic effects and the potential mechanisms. We found that TCBPA could interfere with nervous and cardiovascular development through focal adhesion and extracellular matrix-receptor (ECM-receptor) interaction pathways through transcriptomic analysis. Behavioral and biochemical analysis results indicated abnormal swimming behavior of zebrafish larvae. Morphological observations revealed that TCBPA could cause the loss of head blood vessels. Metabolomic analysis showed that arginine-related metabolic pathways were one of the main pathways leading to TCBPA developmental toxicity. Our study demonstrated that by using omics, TCBPA was shown to have neurological and cardiovascular developmental toxicity and the underlying mechanisms were uncovered and major pathways identified.

Icariin promotes angiogenesis in glucocorticoid-induced osteonecrosis of femoral heads: In vitro and in vivo studies.

The injury and dysfunction of the femoral head microvascular endothelial cells are associated with the pathogenesis of glucocorticoid‐induced osteonecrosis of the femoral head (ONFH). Reports indicate that icariin (ICA) can enhance vascular roles and also inhibit endothelial cell dysfunction. However, it still remains unclear whether ICA can promote angiogenesis in glucocorticoid‐induced ONFH. In this study, we investigate this hypothesis through in vitro and in vivo experiments. Results showed that 0.1 mg/mL hydrocortisone significantly suppressed bone microvascular endothelial cells (BMECs) proliferation while ICA at 10−5 mol/L reversed this inhibition. ICA significantly promoted BMECs migration, tube formation, the angiogenesis‐related cytokines expression and the activation of Akt. Furthermore, ICA enhanced Bcl‐2 expression but diminished Bax expression. According to in vivo results, rats with ICA treatment exhibited a lower ratio of empty lacunae, higher volume of blood vessels and more CD31‐positive cells. This study revealed that ICA promotes angiogenesis of BMECs in vitro and improves femoral head blood vessel volume of rats treated with glucocorticoid, suggesting the efficacy of ICA in the prevention of glucocorticoid‐induced ONFH.

Prediction of mechanical behavior of cartilaginous infant hips in pavlik harness: A subject-specific simulation study on normal and dysplastic hips.

In dysplastic infant hips undergoing abduction harness treatment, cartilage contact pressure is believed to have a role in therapeutic cartilage remodeling and also in the complication of femoral head avascular necrosis. To improve our understanding of the role of contact pressure in the remodeling and the complication, we modeled cartilage contact pressure in cartilaginous infant hips undergoing Pavlik harness treatment. In subject-specific finite element modeling, we simulated contact pressure of normal and dysplastic hips in Pavlik harness at 90° flexion and gravity-induced abduction angles of 40°, 60° and 80°. We demonstrated that morphologies of acetabulum and femoral head both affected contact pressure distributions. The simulations showed that in Pavlik harness, contact pressure was mainly distributed along anterior and posterior acetabulum, leaving the acetabular roof only lightly loaded (normal hip) or unloaded (dysplastic hip). From a mechanobiological perspective, these conditions may contribute to therapeutic remodeling of the joint in Pavlik harness. Furthermore, contact pressure increased with the angle of abduction, until at the extreme abduction angle (80°), the lateral femoral head also contacted the posterior acetabular edge. Contact pressure in this area could contribute to femoral head avascular necrosis by reducing flow in femoral head blood vessels. The contact pressure we simulated can plausibly account for both the therapeutic effects and main adverse effect of abduction harness treatment for developmental dysplasia of the hip. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

The effect of combined treatment with transcranial direct current stimulation on cerebral blood flow in patients with cerebral palsy

There is a close link between the activity of the brain and cerebral blood supply. Transcranial direct current stimulation (tDCS) modulates the activity of the cerebral cortex and thus affects the cerebral blood flow. The aim of the study was to investigate the effect of combined treatment with tDCS on cerebral blood flow in patients with cerebral palsy (CP). Materials and Methods. 60 patients with various forms of cerebral palsy were examined and received the course of treatment. The comparison group was formed from 30 children who received the course of basic medical and rehabilitation procedures. The main group included 30 children who, in addition to the same therapy, received a course of tDCS. A transcranial Doppler ultrasound examination of head blood vessels was used for the study of cerebral hemodynamics in children with cerebral palsy before and after combined treatment with tDCS. Results. tDCS reduced asymmetry coefficient of blood flow velocity in the middle cerebral arteries (MCA) by 12.3 %, whereas in the comparison group only by 2.5 %; in the anterior cerebral artery (ACA) – 9.5 %, while in the comparison group – 0.8 %. tDCS significantly reduced the high mean blood flow velocity per cycle (MFV) in the basilar artery (BA), MCA and ACA (21.7 %, 18.3 % and 7.8 %, respectively); in the comparison group no statistically significant positive dynamics was observed. tDCS significantly increased the low MVF in the BA, MCA and ACA (29.7 %, 21.2 % and 9.7 % respectively); a statistically significant increase of MVF by 9.9 % was only in the CMA in the comparison group of patients. Conclusions. Our data indicate that the use of tDCS in the combined treatment of CP patients improves cerebral hemodynamics in 87 % of patients, in contrast to 52 % in the comparison group. The addition of transcranial direct current stimulation method to the complex treatment of patients with cerebral palsy improves the effectiveness of treatment and may also positively influence on the disease clinical course.

Influencing factors of prognosis of acute intraoperative encephalocele in craniotomy of brain injury patients

Objective To explore the influencing factors of acute intraoperative encephalocele in craniotomy of brain injury patients. Methods A retrospective analysis about 168 cases of acute encephalocele in craniotomy surgery in patients with clinical data and follow-up results, according to the Glasgow outcome scale (GOS) group: patients with GOS 1~3 were divided into poor prognosis group (control group), patients with GOS 4~5 were divided into good prognosis group (research group). The second component multiariable Logistic regression analysis method was used for the analysis of patient age, history of heart head blood-vessel, preoperative score (GCS), preoperative pupil, encephalocele reasons, whether reoperation, postoperative GCS score, postoperative pupil, postoperative blood coagulation indexes, blood sugar, etc. Results Good prognosis was observed in 33 cases and poor prognosis in 135. The postoperative GCS, reasons of encephalocele and age were closely related with the prognosis (P<0.05). Conclusions The overall prognosis of traumatic brain injury patients with acute encephalocele during craniotomy was poor. The postoperative GCS, reasons of encephalocele and age could be used as important indexes for prognosis evaluation. Key words: Traumatic brain injury; Encephalocele; Prognosis

Analysis of morphological variation of the internal ophthalmic artery in the chinchilla (Chinchilla laniger, Molina)

The aim of this investigation was the analysis of the variability within the internal and external ophthalmic artery in the chinchilla (Chinchilla laniger, Molina). The head vasculature of 65 individuals was analysed, with particular emphasis on the internal ophthalmic artery originating from the central and rostral part of the cerebral arterial circle. Head blood vessels were filled with acrylic latex for vascular corrosion casting. The results showed ten variants of blood supply for the orbit, with a predominance of the first variant (66.1%) = bilateral presence of the external ophthalmic artery originating from the maxillary artery. Other variants differed in symmetry and asymmetry, sites of origination and the coexistence of both internal and external arteries. Vascularisation of the brain in chinchillas originates mainly from the vertebra-basilar system. The observed variability seems to confirm the role of the basilar artery in the arterial blood supply of the brain in this species.

Research on Food Manufacturing with Effect of Cooking on Lotus Roots Absorbing Cholesterol and Saturated Fatty Acids

Target For the lotus roots have caring characteristics on inventions of obesity and preventions of“Three Height”, which compared the test on the absorption function of lotus roots absorbing cholesterol and fatty acids after cooking ,provide the basis for reasonable edible method and develop the special adsorbent.Method The lotus roots were steamed, boiled, fried and microwaved,then tested and compared the changes of the adsorption of cholesterol and fatty acid, studied on the influence factors of adsorption, and got the processing method of high adsorbability.Results ① With the extension of adsorption time，the amount of cholesterol absorbed by all samples surges，which peaks after 1-1.5h. While as more lotus roots are used ,the amount of cholesterol absorbed declines, which compared absorption by different methods: microwaving ＞ poaching＞steaming＞frying. ② The lotus roots absorbed saturated fatty acids＞the lotus roots absorbed unsaturated fatty acids,which compared absorption by different methods: steaming＞poaching＞microwaving＞frying.Conclusion In order to treat disease of heart head blood-vessel and ensure to play a role in absorption and excretion on cholesterol and saturated fatty acids after eating ,Lotus roots are promoted to use cooking recipes of microwaving,steaming and poaching instead of frying .

Intravascular Papillary Endothelial Hyperplasia: Diagnostic Sequence and Literature Review of an Orofacial Lesion

Intravascular papillary endothelial hyperplasia or Masson's tumor is a rare reactive disease of vascular origin characterized by exuberant proliferation of endothelial cells notably occurring within blood vessels of head, neck, and extremities. The importance of this entity is its ability to mimic a variety of diseases both benign and malignant in the orofacial region. Here, we present a case of Masson's tumor within the masseter muscle in a 40-year-old female with emphasis on the sequential investigative procedures performed to diagnose this entity.

Detection Technology and Application of SpO&lt;sub&gt;2&lt;/sub&gt; Based on Dual Wavelength System

With the development and progress of the society, people's physical health is getting higher and higher. Enjoy superior in material life and all kinds of disease of heart head blood-vessel also shows high trend. To solve these problems are involved in one of the important parameters- the blood oxygen saturation of body. For application object and environment, The author used fixed double wavelengths red light infrared collection principle. On the premise of not traumas continuous real-time detection.The research, with AT89S52 SCM as cybernetics core, analyses each unit of the structure function and the realization methods and completes commissioning of hardware system .The research,with AT89S52 SCM as cybernetics core, analyses each unit of the structure function and the realization methods and completes commissioning of hardware system. The way can erasure reliability and anti-interference, but also reduce the cost of instrument, in order to fulfill the demand of public. tests show that: this device has speedily test, high sensitivity and accuracy to the requirements of experiment calibration

Migration and Accumulation of Primordial Germ Cells of Early Embryo in Quail

In this research, we have identified primordial germ cells (PGCs) in quail embryo using Quail Hemangioblastic Lineage (QH1) monoclonal antibody analysis. Quail PGCs originated from the opaca of unincubated blastodisc, and then transferred to the pellucida and the germinal crescent. At 27 hours post-incubation, a few PGCs first appeared in blood vessels of the pellucida, where many PGCs accumulated at 36 hours post-incubation. The PGCs scattered or clustered from head to omphalo mesenteric and mainly settled down in the mesenchymal blood vessels of head at 45 hours post-incubation. The size of PGCs population increased significantly (P<0.05) from stage XII (12.8±4.82 µm) to primitive streak stage (106.7±8.74 µm) and from Head process stage (95.8±19.74 µm) to tenth somite stage (199.4±19.97 µm). It is concluded that the PGCs scattered in the head area before migration to the germinal crescent and distributed randomly in both gland. The number of PGCs varied at different stages with two peaks, primitive streak stage (18 hours post-incubation) and tenth somite (36 hours post-incubation).

Oxidative injury to blood vessels and glia of the pre-laminar optic nerve head in human glaucoma

Glaucoma is a leading cause of irreversible world blindness. Oxidative damage and vascular injury have been implicated in the pathogenesis of this disease. The purpose of this study was to determine in human primary open angle glaucoma whether oxidative injury occurs in pre-laminar optic nerve blood vessels and glial cells. Following IRB approval, sections from post-mortem primary open angle glaucoma eyes ( n = 5) with mean age of 77 ± 9 yrs (±SD) were compared to normal control eyes ( n = 4) with mean age 70 ± 9 yrs (Eye Bank of Canada). Immunostaining with nitrotyrosine, a footprint for peroxynitrite-mediated injury, was performed and sections were double-labeled with markers for vascular endothelial cells, perivascular smooth muscle cells, and astrocytes with CD34, smooth muscle actin (SMA), and glial fibrillary acidic protein (GFAP), respectively. Immunostaining was captured in a masked fashion using confocal microscopy, and defined regions of interest for blood vessels and glial tissue. Intensity measurements of supra-threshold area in pixels as percent of the total number of pixels were calculated using ImageJ (NIH) and compared using two-tailed Mann–Whitney nonparametric tests between glaucoma and control groups. Colocalization coefficients with cell-specific markers were determined and compared with random coefficients of correlation. Increased nitrotyrosine immunoreactivity was observed in pre-laminar optic nerve head blood vessels of primary open angle glaucoma eyes compared to controls and this difference was statistically significant (1.35 ± 1.11% [±SD] vs. 0.01 ± 0.01%, P = 0.016). NT-immunoreactivity was also increased in the glial tissue surrounding the pre-laminar optic nerve head in the glaucoma group and compared to controls, and this difference was statistically significant (18.37 ± 12.80% vs. 0.08 ± 0.04%, P = 0.016). Colocalization studies demonstrated nitrotyrosine staining in vascular endothelial and smooth muscle cells, in addition to astrocytes. Correlation coefficients for CD34, SMA, and GFAP were 0.37, 0.52, and 0.64, respectively. Oxidative injury is present in blood vessels and astrocytes in the pre-laminar optic nerve head in human primary open angle glaucoma. Peroxynitrite-mediated oxidative injury, whether primary or secondary, may contribute to the pathobiology of glaucoma disease.

B206 医療画像に基づく脳血管血流シミュレーション(B2-2 生体流体工学1)

B206 医療画像に基づく脳血管血流シミュレーション(B2-2 生体流体工学1)

The angiogenic potentials of the cephalic mesoderm and the origin of brain and head blood vessels

We have used two molecular markers to label blood vessel endothelial cells and their precursors in the early avian embryo. One marker, called Quek1, is the avian homologue of the mammalian VEGF receptor flk-1 and the other is the MB1 QH1 monoclonal antibody. Quek1 is expressed in a subset of mesodermal cells from the gastrulation stage. Quek1 positive cells later form blood vessel endothelial cells and express the MB1 QH1 antigen which is specific for endothelial and hemopoietic cells of the quail species. These two markers allowed us first to show that the cephalic paraxial mesoderm has angiogenic potentials which are much more extended than its trunk counterpart (the somites). Secondly, the origin of the endothelial cells lining the craniofacial and head blood vessels was mapped on the 3-somite stage cephalic mesoderm via the quail-chick chimera technique, in which well defined mesodermal territories are exchanged between stage-matched embryos of both species in a strictly isotopic manner. We found that the anterior region of the cephalic paraxial mesoderm is largely recruited to provide the forebrain and the upper face with their vasculature. This means that large volumes of tissues are vascularized by a discrete region of the cephalic mesoderm, the fate of which is otherwise to give rise to muscles. The widespread expansion of the angiogenic cells arising from the anterior paraxial mesoderm must be related to the high growth rate of the anterior region of the neural primordium, yielding the telencephalon and of the neural crest-derived facial structures which are themselves devoid of angiogenic potencies. In contrast, in the more posterior mesodermal territories, the angiogenic cells vascularize the corresponding levels of the brain, the tissues (bones and muscles) that they otherwise produce, as well as the neural crest derivatives of the hypobranchial region. Mixing of angioblasts of host and donor is essentially restricted to the marginal zones of the territory so defined, showing that endothelial cells of each mesodermal region vascularize tissues according to a restricted and well defined pattern.

Primitive-Streak Origin of the Cardiovascular System in Avian Embryos

The origin of the avian cardiovascular system from the primitive streak has been mapped by the construction of quail/chick transplantation chimeras, the use of QH-1 (an antiquail endothelial/endocardial cell marker), and injections of a vital fluorescent dye (DiI) into the primitive streak. Our studies reveal that the prospective heart, including its endocardial and myocardial layers and the adjacent parietal pericardium, occupies much of the rostral half of the primitive streak at early primitive-streak stages of gastrulation. The heart originates from the primitive streak in roughly rostrocaudal sequence (i.e., the prospective bulbus cordis arises more rostrally in the streak than does the prospective ventricle, which in turn arises more rostrally than does the prospective sinus venosus), and all layers of the heart at each of its rostrocaudal subdivisions originate in concert from the same level of the primitive streak. Moreover, all rostrocaudal levels of the primitive streak at gastrula and neurula stages contain prospective endothelial cells. Again, these cells are rostrocaudally ordered within the streak, such that head blood vessels (both arteries and veins) arise at more rostral streak levels than do trunk blood vessels. Ingression of cardiogenic cells is complete by midprimitive-streak stages, and the position formerly occupied by prospective cardiogenic cells within the streak becomes occupied by ingressing prospective somitic cells. Additional studies on the state of commitment of prospective cardiogenic and endothelial cells during their ingression through the primitive streak are underway.

Regulative ability of the prospective cardiogenic and vasculogenic areas of the primitive streak during avian gastrulation.

Four types of microsurgical experiments were conducted to analyze heart and blood vessel development during gastrula stages of avian embryos, stages during which prospective cardiogenic and vasculogenic cells reside within the primitive streak. Experiments addressed whether cells not normally destined to form heart could form heart when given the opportunity to do so and vice versa; cells destined to form rostral levels of the heart (or, alternatively, head blood vessels) could form caudal levels of the heart (or, alternatively, trunk blood vessels) and vice versa; the early endoderm imparts rostrocaudal organization to the heart and associated blood vessels; and ingression of cells from the primitive streak and their subsequent migration into the mesodermal mantle is a cell-autonomous event for migrating cells. Our results demonstrate the lability of prospective cardiogenic and vasculogenic cells of the primitive streak, both in terms of the type of mesodermal structures they are capable of forming (or of being formed from) and in terms of the rostrocaudal patterning of the cardiovascular system. In addition, our results show that cell ingression and migration is directed by environmental cues and is not a cell-autonomous process for migrating cells. Finally, our results suggest that patterning of the cardiovascular system occurs after cells enter the mesodermal mantle, presumably through cell-cell inductive interactions. However, the early endoderm is not the primary source of the patterning influence. What is the source remains to be established.