He-Ne Laser Irradiation Research Articles

Study on the effect of laser irradiation on wheat (Triticum aestivum L.) variety PBW-373 seeds on zinc uptake by wheat plants

The present study was carried out to investigate the effect of He-Ne laser irradiations on wheat (Triticum aestivum L.) variety PBW-373 seeds on uptake and internal distribution of zinc ion in wheat plants for different irra- diation time in soil culture. The transport of zinc from root system to shoot and grain was studied by incorporating radioactive zinc as zinc chloride (Zn * Cl2) with the nutrient solution to the plants. The 65 Zn activity absorbed by the matured plants and distributed in different parts (root, shoot and grain) was measured with a calibrated NaI (Tl) gamma ray spectrometer. The results led the conclusion that translocation of zinc from shoot to grain in mature plants in treated soaked seeds showed a better result in comparison to dry treated and control seeds. The utilization of zinc from roots to shoots was higher in the plants grown from dry seeds in all given treatments and from shoot to grain was best in soaked seeds at 2 min irradiation period.

Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes

The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 μmol/L HgCl2, was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2for 5 min. Comprehensive morphological parameters of an intact cell such as contact area, perimeter, roundness and erythrocyte elongation index (EEI) were measured to characterize erythrocyte deformability with fast micro multi-channel spectrophotometer. It was observed that the dosage of LHNL improvement of the morphological parameters of dehydrated erythrocytes was morphological-parameter-dependent, but the Bunsen-Roscoe rule did not hold for roundness. The LHNL at 4.4 mW/cm2for 5 min significantly improved the contact area (P<0.05) and EEI (P<0.05) of the dehydrated erythrocytes, but the improvement was significantly inhibited by 0.2 μmol/L HgCl2(P<0.05). It was concluded that AQP-1 might mediate the effects of LHNL on erythrocyte deformability, which supports the membranotropic mechanism of PBM.

氦氖激光照射对大鼠实验性牙移动牙周组织中环氧化酶-2表达的影响

氦氖激光照射对大鼠实验性牙移动牙周组织中环氧化酶-2表达的影响

Induction of primitive pigment cell differentiation by visible light (helium–neon laser): a photoacceptor-specific response not replicable by UVB irradiation

Solar lights encompass ultraviolet (UV), visible, and infrared spectrum. Most previous studies focused on the harmful UV effects, and the biologic effects of lights at other spectrums remained unclear. Recently, lights at visible region have been used for regenerative purposes. Using the process of vitiligo repigmentation as a research model, we focused on elucidating the pro-differentiation effects induced by visible light. We first showed that helium-neon (He-Ne) laser (632.8 nm) irradiation stimulated differentiation of primitive pigment cells, an effect not replicable by UVB treatment even at high and damaging doses. In addition, significant increases of mitochondrial DNA copy number and the regulatory genes for mitochondrial biogenesis were induced by He-Ne laser irradiation. Mechanistically, we demonstrated that He-Ne laser initiated mitochondrial retrograde signaling via a Ca(2+)-dependent cascade. The impact on cytochrome c oxidase within the mitochondria is responsible for the efficacy of He-Ne laser in promoting melanoblast differentiation. Taken together, we propose that visible lights from the sun provide important environmental cues for the relatively quiescent stem or primitive cells to differentiate. In addition, our results also indicate that visible light may be used for regenerative medical purposes involving stem cells.

He‐Ne Laser‐Induced Improvement in Biochemical, Physiological, Growth and Yield Characteristics in Sunflower (Helianthus annuus L.)

The water-soaked seeds of sunflower were exposed to low power continuous wave He-Ne laser irradiation of energies 0, 100, 300 and 500 mJ to evaluate the effect on various biochemical, physiological, growth and yield parameters of sunflower. The experiments which consisted of four replicates arranged in a completely randomized design (CRD) were carried out under the greenhouse conditions. The physiological attributes like, photosynthetic rate (A), transpiration rate (E), intrinsic CO(2) concentration (C(i) ), stomatal conductance (g(s)), chlorophyll a and b contents, relative membrane permeability and leaf water (ψ(w)), osmotic (ψ(s)) and turgor (ψ(p)) potentials, relative water contents and leaf area increased significantly as compared to control due to He-Ne treatment of seeds. The activities of superoxide dismutase, peroxidase and catalases and contents of total soluble proteins, malondialdehyde, proline and leaf total phenolic also increased due to laser treatment. Significant increase in growth parameters of sunflower like shoot fresh and dry masses, root fresh and dry masses, root and shoot lengths, number of leaves per plant and stem diameter has also been observed. The contents of K, Ca and Mg in shoot and root were also increased and an overall increase of up to 28.12% was observed due to laser treatment.

Analog of the Umov experiment in a birefringent medium

Classical demonstration experiment on visualization of light polarization plane rotation in an optically active medium is the Umov experiment [1–4]. When a collimated polarized light beam passes through an optical cell filled with a sugar solution in water and radiation scattered at an angle of 90o to the direction of beam propagation is observed, a bright dashed line typical of light scattered in an isotropic medium is seen rather than a solid line. The essence of the phenomenon is that when the beam passes through the active medium, its polarization plane is rotated. Due to the fact that the electromagnetic waves are transverse in character, the scattered light intensity vanishes when the direction of light polarization plane coincides with the observation direction. Because the effect is rather weak, to observe several tracks, the cell length must be no smaller than ∼1 m (even when it is filled with the concentrated sugar solution). Analogous character of scattering is observed in crystalline media [5–7] in which the beam polarization state also changes during beam propagation. However, because the birefringence effect in crystals is generally relatively strong, the similar scattering pattern can be seen with naked eye only in the direction close to the optical crystal axis where its intensity is low. In the present work, experiments on light scattering in an Icelandic spar crystal and a Plexiglas bar are described. Linearly polarized He-Ne laser or laser pointer radiation with power 10 mW P ≤ passed through the spar crystal of low optical quality (red and green pointers were used). To observe a scattering track stripe in the crystal, the laser beam was incident in the direction close to the optical crystal axis (Z-cut). Figure 1a shows a photograph of the scattering pattern in the spar crystal 4.2 cm long. Since the birefringence intensity in the crystal depends on the direction of light propagation, smooth crystal rotation causes changes in the scattering stripe period. They were observed in our experiment. The bright dashed scattering straight line can also be observed in plexiglass where, as a rule, small residual birefringence is always presented [7]. Figure 1b shows a photograph of light scattering pattern in the organic glass rod 50 cm long. In both cases, no professional polishing of specimen sides is required for clear visibility of tracks. Their simple coating is sufficient. The birefringence intensity of the medium n Δ is related to the track period Λ by the formula / n Δ = λ Λ ; it is determined exactly enough. Based on observations of the scattering pattern through a polaroid that is rotated, it is easy to verify that radiation scattered at an angle of 90o is almost completely polarized. The above-described experiments whose ideology and observed pattern are analogous to the Umov experiment are performed with simple accessible materials. The track stripe of scattered light in both cases is well seen by naked eye in slightly turbid specimens in a darkened room. The experiments on measuring the optical anisotropy of the medium for light scattering can provide the basis for a laboratory work or a demonstration experiments for a large audience.

Laser Therapy of Irradiated Traumatized Skeletal Muscles and State of Immune System in Animals

Problems statement: This study was designed to examine the effect of laser therapy on regeneration of traumatized skeletal muscles and on dynamics of immune responses in thymus and bone marrow under ionizing radiation. Approach: After local X-ray radiation of both rat gastrocnemius muscles at a dose 20 Gy or after total γ-irradiation of animals at a semiletal dose 6 Gy, the muscles were damaged considerably as contractile organ (full cut across muscle). Then laser therapy was carried out with a helium-neon laser (632.8 nm) or infrared pulsed laser (890 nm), 10 treatments by 3 min during the first 15 or 30 days after trauma.The investigations were performed using histological, biochemical, cytological, cytogenetic and morphometric methods. Results: The received data showed that under ionizing radiation, He-Ne and IR laser irradiation stimulated the regeneration of skeletal muscles, improved the healing of skin-muscle wound and stimulated the prosesses of postradiation recovery in cells of the thymus and bone marrow. The regenerative activity of radiated traumatized muscles and the level of reduction of cytogenetic damages in bone marrow and the thymus varied depending on the period for which regenerative muscles were exposured to laser light, as well as the optical characteristics of lasers and the degree of radiation damages. The early appearance of reactive changes in structure of the thymus and the display them during 60 days point to increasing function of organ. Conclusion: Under local and total ionizing radiation and laser therapy of solely damaged skeletal muscles, positive dynamics of regeneration processes and improvement of cytological and cytogenetic indexes for main immune organs (the thymus and bone marrow) were observed. Herewith, the functional loading on thymus increased. We suggest that the findings should be considered in clinical practice.

Effects of He-Ne laser irradiation on the storage of turkey semen

Maintenance or improvement of sperm quality during storage could prevent the loss of fertilizing capacity associated with stored turkey semen. Therefore the optimization of stored turkey semen could be useful to breeder industry since the commercial production of this bird relies almost entirely on artificial insemination. Previous research have shown that He-Ne laser irradiation in mammalian sperm increased the motility (Stato, 1986), decreased the mortality, promoted the acrosome reaction, which have a pivotal role in assisted fecundating programmes as therapy for resolving infertility in domestic animals

提高拮抗菌WB3对番茄灰霉病菌拮抗能力的HeNe激光与氯化锂复合诱变技术

He-Na laser(λ=632.8 nm,P=12 mW) licl was used to irradiate B.subtilis WB3 which had an antagonistic ability on Botrytis cinerea.14 strains that have prominent antagonistic effects were obtained,and the maximum inhibitory bandwidth is 15.7 mm.According to the mortality rates and the size of antagonistic of single mutagenesis group,the optimization condition of irradiation time of 30 min and the density of licl of 1.2% were obtained.After multiplicity mutation,the max inhibiting belt width is L8 that raised more than 14%,however single He-Ne laser and licl irradiation could only raise 8.8% and 9%.The strains grew rapidly with stable heredity.The proposed experimental results the He-Na laser licl mutagenesis of Bacillus subtilistest WB3 show a broad application prospect for the biological control of botrytis cinerea.

LLLT AND PDT

This Letter relates to the paper of Yuichi Miyamoto et al., “EFFECT OF 630-NM PULSED LASER IRRADIATION ON THE PROLIFERATION OF HeLa CELLS IN PHOTOFRIN®-MEDIATED PHOTODYNAMIC THERAPY” published recently in Vol. 20 (2). The authors investigated changes in cell proliferation following PDT using 630nm laser irradiation which was clinically found to induce no remarkable cell injury. They found that the viability of irradiated HeLa cells was greater than that of non-irradiated cells, i.e. an LLLT effect. They derived the conclusion that in PDT when light doses become remarkably low (deep within the tissue), pronounced LLLT effects and no cytotoxic effects are obtained. We would like to draw the attention of the readers to an old paper of us1) where we found that the proliferation rate of fibroblasts enriched with small amounts of hematoporphyrin derivatives (HPD) was increased following low intensity HeNe laser irradiation. PDT and LLLT are two edges of the same sword. In PDT, exogenous photosensitizers are introduced into the cells and then irradiated with wavelengths of visible or near infra-red (NIR) light to produce high amounts of ROS that are lethal to the cells. In LLLT, light is absorbed by endogenous cellular photosensitizers such as cytochromes, flavins, porphyrins and NADH.2) Since the amount of endogenous cellular photosensitizers is relatively small, low concentrations of ROS are generated. It is well established that ROS in minute concentrations stimulate cellular functions like proliferation.3) The fact that exogenous porphyrins combined with very low energy visible light enhance proliferation must be taken into account when using PDT. Cancerous cells enriched with small amounts of photosensitizers (like those at the border of a malignant tumor) may proliferate better after irradiation. For the same reason, LLLT should be avoided in the presence of cancerous cells.4) LLLT and PDT can be viewed as different zones of the well-known Arndt-Schultz (A-S) curve, (see e.g. Fig. 10 in the review of T. Ohshiro5). Zone 1, ascending part of the A-S curve: weak to moderate light doses and endogenous photosensitizers:, or very weak light doses adding exogenous photosensitizers, or moderate light doses adding minute amounts of exogenous photosensitizers lead to stimulation as in LLLT. Zone 2, the A-S curve descends back to the baseline: in this Zone, strong light doses and endogenous photosensitizers or moderate light doses adding small amounts of exogenous photosensitizers lead to neutralizing the stimulation obtained in Zone 1. Zone 3, the A-S curve descends below the baseline: in Zone 3, a further increase of the combined light and exogenous photosensitizer stimuli dosage leads to PDT.

Development and evaluation of an optical fibre-based helium-neon laser irradiation system for tissue regeneration: A pilot study

Low level laser therapy is being extensively used to treat various medical ailments including wound healing. In the present study, an optical fibre-based helium-neon (He-Ne) laser irradiation system was designed, developed and evaluated for optimum tissue repair on mice excision wounds. Circular wounds of 15 mm diameter were created on the dorsum of animals and single exposure of uniformly distributed laser beam was administered at 1, 2 and 3 J/cm2 to the respective test groups with suitable controls. Progression of healing was monitored by measuring wound contraction and mean healing time. Significant reduction in wound size and mean healing time (p <0.001) were observed in the test groups for the laser dose of 2 J/cm2 compared to the unilluminated controls, suggesting the suitability of this dose.

Treating chronic colonitis diarrhea by He-Ne laser irradiation of acupoints: A report of 200 cases

Objective To compare the effect of treating chronic colonitis diarrhea (CCD) using He-Ne laser irradiation of acupoints with routine drug treatment. Methods Two hundred patients with CCD were randomly divided into a laser irradiation group (laser group) and a routine drug treatment group (control group) with 100 patients in each group. The laser group patients were treated with a He-Ne laser ( power density 478 mW/cm2 )through coupling fiberoptic cable radiating onto the Shenque (REN8) , bilateral Tianshu (ST25) and Dachangshu (BL25) acupoints, each for 10 min daily for 10 days. Control group patients were treated with routine drugs such as antibiotics, astringents, antidiarrheals, antispasmodic analgesics, vitamins and Chinese herbal enemas. Three months after treatment the patients were examined by fibercoloscope to evaluate the therapeutic efficacy in the two groups. Results In the laser group 66 cases (66%) experienced a short-term cure, 27 cases (27%) improved and7 cases (7%) showed no effect. In the control group 33 cases (33%) were short-term cures, 43 cases (43%) improved and 24 cases (24%) had no effect. These differences were statistically significant. In both groups the effects for patients with short histories was better than for those with longer histories. In the laser group the pathological changes in the distal colon were significantly better than in the proximal colon, but this difference wasn't significant in the control group. The average days of total treatment to achieve short-term cure and improvement in the laser group were significantly less than in the control group. In the laser group the average days to the disappearance of abdominal pain and diarrhea in the short-term cure patients of was significantly fewer than in the control group. Conclusions Laser irradiation was more effective than routine drug treatment, especially for stopping diarrhea, spasmolysis and analgia. Key words: Lasers; Acupoints; Colouitis; Diarrhea

Muscle regeneration and the state of the thymus in adult rats under laser irradiation and alloplasty of newborn gastrocnemius muscle and diaphragm

The regeneration of adult rat gastrocnemius muscles has been studied under implantation of new-born rat muscles into area of muscle trauma. Alloplasty was performed using minced gastrocnemius and diaphragm muscles, which differs at birth in animals by degree of differentiation. The rat-recipient area of alloplasty was subjected to He-Ne laser radiation before operation, with the aim of reducing the immune response to allogenic muscle tissue. It has been shown that the number of regenerating myofibers produced from implanted gastrocnemius muscles is more than in alloplants from diaphragms. However, the formation of cartilage, bone, and adipose tissue foci was observed in the alloplastic region throughout the whole regeneration period. After implantation of minced diaphragm muscles, cartilage nodes were observed only in 7-day regenerates. At the end of observation, in the first instance, the area of muscle trauma in adult rat muscles was replaced by adipose tissue, even in the case of initial laser irradiation. After the implantation of diaphragm muscles, the area of trauma was filled with regenerating muscle tissue.

Influence and mechanism of He-Ne laser on scar formation of filtration canal after trabeculectomy in rabbit.

To investigate the influence of He-Ne laser on connective tissue growth factor (CTGF) expression and collagen formation of fibroblast in filtration site after trabeculectomy in rabbit, and to discuss the mechanism for preventing scar formation with He-Ne laser in vivo. The upper nasal limbus area next to the upper rectus muscle in right eyes received 10 minutes He-Ne laser irradiation (200mW/cm(2)) every day for three days, the left eyes served as control. Twenty-four hours after the last irradiation, both eyes of the rabbits were took trabeculectomy surgery. The expressions of CTGF in the filtration area were tested on the 7(th), 14(th) and 28(th) day after surgery and collagen density was tested on the 14(th) and 28(th) day after surgery. Each of the time point had 7 rabbits. The expression of CTGF was lower than that of the control group's on the 7(th) and 14(th) day after trabeculectomy surgery (P=0.01, P=0.005). When examined on the 14(th) and 28(th) day, the collagen density of irradiation group were significantly lower than that of the control group's (P=0.013, P=0.01). Pretreating the filtration area with 200mW/cm(2) He-Ne laser may be helpful in preventing scar formation after trabeculectomy in rabbit, possibly due to downregulation of the expression of CTGF and collagen synthesis in fibroblasts. He-Ne laser may be developed into a new scar preventing method in filtration surgery.

Kinetic Analysis and Modeling of Daptomycin Batch Fermentation by Streptomyces roseosporus

In this study, Streptomyces roseosporus was subjected to helium-neon (He-Ne) laser (632.8nm) irradiation to improve the production ability of extracellular antibiotic daptomycin. Under the optimum irradiation dosage of 18mW for 22min, a stable positive mutant strain S. roseosporus LC-54 was obtained. The maximum A21978C (daptomycin is a semisynthetic antimicrobial substance derived from the A21978C complex) yield of this mutant strain was 296mg/l, which was 146% higher than that of the wild strain. The mutant strain grew more quickly and utilized carbohydrate sources more efficiently than the wild strain. The batch culture kinetics was investigated in a 7l bioreactor. The logistic equation for growth, the Luedeking-Piret equation for daptomycin production, and Luedeking-Piret-like equations for carbon substrate consumption were established. This model appeared to provide a reasonable description for each parameter during the growth phase and fitted fairly well with the experiment data.

Effects of Different Doses of Low Power Continuous Wave He–Ne Laser Radiation on Some Seed Thermodynamic and Germination Parameters, and Potential Enzymes Involved in Seed Germination of Sunflower (Helianthus annuus L.)

In this study, water-soaked seeds of sunflower were exposed to He-Ne laser irradiation of different energies to determine whether or not He-Ne laser irradiation caused changes to seed thermodynamic and germination parameters as well as effects on the activities of germination enzymes. The experiment comprised four energy levels: 0 (control), 100, 300 and 500mJ of laser energy and each treatment replicated four times arranged in a completely randomized design. The experimentation was performed under the greenhouse conditions in the net-house of the Department of Botany, University of Agriculture, Faisalabad. The seed thermodynamic parameters were calculated according to seed germination thermograms determined with a calorimeter at 25.8°C for 72h. Various thermodynamic parameters of seed (ΔH, (ΔS)(e), (ΔS)(c), (ΔS)(e) /Δt and (ΔS)(c) /Δt) were affected significantly due to presowing laser treatment. Significant changes in seed germination parameters and enzyme activities were observed in seeds treated with He-Ne laser. The He-Ne laser seed treatment resulted in increased activities of amylase and protease. These results indicate that the low power continuous wave He-Ne laser light seed treatment has considerable biological effects on seed metabolism. This seed treatment technique can be potentially employed to enhance agricultural productivity.

Effect of HeNe Laser Irradiation on Extracellular Matrix Deposition and Expression of Cytokines and Chemokines in Paracoccidioidomycotic Lesions

Paracoccidioidomycosis is the most prevalent human mycosis in Latin America. Cutaneous lesions are extremely painful and sensitive, and current treatment with antifungal drugs is lengthy and may cause side effects to patients. In this perspective, the helium-neon (HeNe) laser emerges as a novel therapy form due to its ability to heal wounds without changing cell function. In this work, we evaluate the effects of HeNe laser irradiation on extracellular matrix deposition and expression of cytokines and chemokines in cutaneous lesions caused by experimental infection of Balb/c mice. Our results showed decreased levels of pro-inflammatory interleukin (IL)-17 and tumor necrosis factor-alpha, and of anti-inflammatory IL-10 cytokines in lesions exposed to HeNe laser irradiation. Chemokines CCL3 and CXCL10 showed decreased levels in laser-treated lesions, but no significant difference was observed in relation to CCL5 expression. We also detected decreased density of fibronectin and laminin in HeNe laser-treated lesions. Data presented herein support the validity of our previous results suggesting positive effects of HeNe laser in accelerating wound healing in this experimental model. We believe that HeNe laser is a new nonharmful strategy that may be used as adjuvant and/or alternative therapy for improving treatment of paracoccidioidomycotic lesions.

The influence of He-Ne laser on scar formation after trabeculectomy in rabbits.

To investigate the influence of He-Ne lasers on scar formation in the filtration canal after trabeculectomy in a rabbit model, as well as to explore the mechanisms for preventing scar formation when using He-Ne lasers in vivo. Experiment 1: Four groups were established (four eyes in each group). In 12 eyes, the upper nasal limbus area next to the upper rectus muscle received 10 minutes of He-Ne laser irradiation (100, 150, 200mW/cm(2); 60, 90, 120J/cm(2)) every day for three days. Four eyes served as controls. Twenty-four hours after the final irradiation, the rabbits were sacrificed and the irradiated tissue was excised, fixed with paraformaldehyde and tested for proliferating cell nuclear antigen (PCNA), connective tissue growth factor (CTGF) and apoptosis (TUNEL). Experiment 2: Forty-two rabbits were randomly divided into two groups and standard trabeculectomy was performed in the right eyes either after 200mW/cm(2) He-Ne laser irradiation or not in the filtration area. The expression of PCNA and CTGF, apoptosis and collagen density in the filtration area were tested on the 7(th), 14(th) and 28(th) day after surgery. Experiment 1: There were no more PCNA and CTGF positive cells in the He-Ne irradiation group than in the control group. No apoptotic cells were found in either group. Experiment 2: The expression of PCNA and CTGF was lower in the He-Ne irradiation group than in the control group on the 7(th) and 14(th) day after trabeculectomy surgery (P<0.05); no apoptotic cells were detected in either group. Collagen density was significantly lower in the He-Ne irradiation group than in the control group on the 14(th) and 28(th) day after surgery (P<0.05). Pretreating the filtration area with 200mW/cm(2) (120J/cm(2)) of He-Ne laser irradiation may be helpful in preventing scar formation after trabeculectomy, possibly due to the downregulation of the expression of PCNA, CTGF and collagen synthesis in fibroblasts.

Effect of photosensitizers pheophorbide a and protoporphyrin IX on skin wound healing upon low-intensity laser irradiation

The effect of photosensitizer with subsequent He-Ne (632.8 nm; 3 mW/cm2) laser irradiation on experimental skin wound healing has been studied. Pheophorbide a and protoporphyrin IX were used as photosensitizers. It was found that application of the photosensitizer and subsequent laser irradiation, first, decreased the amount and the functional activity of leukocytes in the wound exudate and, second, inhibited the SOD activity as compared with that of the control group. Moreover, pheophorbide and protoporphyrin practically did not affect the total healing period but decreased the length of the inflammation stage. It was supposed that these effects are related to generation of reactive oxygen species during irradiation.

The long road to commercialization of Bt brinjal (eggplant) in India

Exposure to laser irradiation on seeds brings about the changes in agronomical characteristics of the plants. Solanum melongena L. var. Mattu Gulla, a variety of brinjal is of high economic value due to its unique colour and flavour. The aim of the study was to understand the influence of Helium-Neon (He-Ne) laser irradiation on agronomical characters of Solanum melongena L. var. Mattu Gulla in the field conditions. Various growth characteristics including seed germination percentage, survival rate, plant height, number of branches, and flowers and fruits were estimated during different developmental stages of the brinjal. In addition, the chlorogenic acid content of fruits obtained from the laser irradiated seeds were quantified using reversed-phase high-performance liquid chromatography (RP- HPLC). The plants from the seeds irradiated with different doses (20, 25 and 30 J/cm2) of He-Ne laser showed significant enhancement on the growth characteristics when compared to the non-irradiated control groups. He-Ne laser irradiation also improved the yield characteristics of the plants significantly in in vivo conditions in comparison with control group. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed using methanolic extract of matured fruit of Mattu Gulla on HepG2 and fibroblast cell lines. The IC50 values of fruit extract from laser irradiated groups were found to be similar to non-irradiated control groups. Chlorogenic acid content was found to be higher in 20 J/cm2 and lower in 30 J/cm2 treated fruit tissue. The current study thus elucidates the role of He-Ne laser as a biostimulator on brinjal var. Mattu Gulla not only in the in vitro conditions but also in the in vivo field conditions.