HC-A Solution Research Articles

Hypoglycemic effect of p-hydroxycinnamic diesters extracted from carnauba wax powder (Copernicia Prunifera) in alloxan-induced diabetic animals

Diabetes is a chronic non-transmissible disease and the number of cases is increasing every year. Plants appear as an alternative therapy since ancient civilizations. Among the species that have promising pharmacological activities are carnauba (Copernicia prunifera (Miller) H. E. Moore). Carnauba wax powder consists of a mixture of heterogenic compounds, among them, p-hydroxycinnamic diesters (HCA). Therefore, this study aimed to investigate the hypoglycemic effect of HCA in alloxan-induced diabetic animals. Therefore, this study was performed with 50 female Swiss mice, distributed in 5 groups (n = 10). The animals of the negative and positive control groups were treated with water: healthy and diabetic mice, respectively; animals of the HCA groups 25 and 50 were diabetic animals and they were treated with the HCA solution at doses of 25 and 50 mg / Kg, respectively; and with metformin, standard drug, at the dose of 200 mg / kg. After 28 days of HCA treatment, a significant hypoglycemic effect was observed in animals treated with HCA at the lowest dose tested (25 mg / kg). In the present study, HCA shown to be a promising compound with good scientific applicability due to the reduction of glycemia of diabetic animals induced by alloxan.

Application of ureterorenoscope and flexible ureterorenoscope lithotripsy in removing calculus from extracorporeal living donor renal graft: a single-center experience

Here, we reported our clinical application of ureterorenoscope (URS) and flexible URS lithotripsy in stone removal on 10 cases of excised living donor kidney graft. After the extraction of donor kidney by retroperitoneal laparoscopy, the donor graft was perfused with 4 °C HCA solution. Calculus between 2–4 mm were removed intact with lithotomy forceps under direct vision of URS. Larger calculi of >4 mm were fractured with flexible URS combining holmium laser lithotripsy. Fragments of the calculus were extracted with basket extractor and lithotomy forceps. All operations were successful. The operation time was 14–31 min (average 21.2 ± 6.3 min). The kidneys were then transplanted to the recipients using routine procedure. The transplanted kidneys functioned well after transplantation. Gross hematuria resolved 1–4 d after operation (average 2.6 ± 0.9 d). The transplanted kidneys functioned well without early complications such as functional recovery delay and acute graft rejection. The donors and recipients were followed for 12 months. The size of the transplanted kidneys was normal and new stones or urinary obstruction was not seen upon urinary color Doppler ultrasound examination. In conclusion, we believe it is feasible, safe and effective to use URS or flexible URS combining holmium laser lithotripsy on extracorporeal living donor kidney.

The application of polymerized porcine hemoglobin (pPolyHb) in the rat small bowel preservation

Small bowel transplantation (SBTx) has become a standard clinical treatment for short bowel syndrome or irreversible intestinal function failure. Optimum preservation of the organ is essential for the success of transplantation. In this study, pPolyHb was used as an additive to hypertonic citrate adenine solution (HCA) to provide oxygen for rat small bowel transplant. Rat small bowels were preserved in HCA, HCA with pPolyHb, and University of Wisconsin solution (UW) for 12, 24, and 36 h, respectively. The results suggested that the preservation effect of HCA with pPolyHb was comparable with the UW solution, and more effective than the HCA solution.

Cold Ischemia Time Influences Spermatogenesis in a Testicular Ischemia/Reperfusion Injury Model

Objective To evaluate the influence of cold ischemia time on spermatogenesis in a rabbit model of testicular ischemia-reperfusion (I/R) injury. Material and Methods The testicular I/R model was established in 24 male white rabbits. The left testes were preserved using HC-A solution at 0°C to 4°C. Cold ischemia time was 1, 2, 4, and 6 hours. The right testes without vascular occlusion were used as autologous controls. Twenty-four hours after reperfusion, the animals were sacrificed, and samples were obtained at bilateral orchiectomy. Another 8 normal testes were used as normal controls. Testicular tissue Johnsen score, malondialdehyde concentration, and apoptosis index were used to evaluate spermatogenesis. Results The Johnsen score decreased and the apoptosis index increased with the duration of cold ischemia time in the I/R groups. The malondialdehyde concentration in the I/R groups was significantly higher than the sham and normal groups, and was highest at 4 hours of cold ischemia time. Conclusion Testicular I/R injury is highly related to cold ischemia time. In rabbit models, testis transplantation is best performed within 4 hours of cold ischemia with traditional hypothermic protection.

Observation on cold-stored rat Hver flushed with self-designed multi-organ preservation solution

Objective To study the effect of serf-designed multi-organ preservation solution(SMO)on cold-stored rat liver.Methods The rat livers were preserved with SMO solution(group A,n=15),UW solution(group B,n=15)and HC-A solution(group C,n=15),respectively.The livers were transplanted orthotopically after 6-,12-,24-hour preservation.The changes of liver function at hour 12 after transplantation were detected and conditions of the survived rats at day 14 after transplantation were observed.Results There was no morphological change of the livers in group A within 24 houm.The total bilimbin,alaninetransaminase,and hyaluronic acid increased in group A and B as the preservation time increased,but the levels of the 3 indexes of group A were significant lower than those in group C(F=49.027,70.280,34.349,71.532,446.544,303.408,P＜0.05).No rat djed in group A 14 days after liver transplantation.Conclusions SMO and UW solution have similar effect on protecting the liver.and the protective effect of the 2 solutioas was better than that of HC-A solution. Key words: Liver transplantation; Organ preservation solution; Rat

Comparative study on the effects of self-made liver preservation solution on secretion of ICAM-1 and NO in rats

In order to study the effect of self-made liver preservation solution on liver preservation by comparing with UW solution and HC-A solution, the self-made liver preservation solution (SM) and perfusion solution were prepared under the aseptic conditions. The isolated non-circulated perfusion rat liver model was established. According to the different preservation solutions, the rats were randomly divided into UW group, SM group and HC-A group. The three groups were divided into 6 subgroups according to the preservation duration (n=6 in each group). The transferase in liver perfusion solution and intercellular adhesion molecule-1 (ICAM-1) and nitric oxide (NO) in liver tissues were determined at 2, 8 and 24 h respectively. The results showed that the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) had no significant difference between SM group and UW group, but significantly lower than in HC-A group. The levels of ICAM-1 and NO were increased simultaneously in SM group and UW group (P>0.05), but there was significant difference as compared with HC-A group (P<0.05). At the same time point, the level of ICAM-1 was higher in SM group than in UW group, but NO was lower. The preservation effect of SM solution is the same as UW solution, but better than HC-A solution.

Optimizing effect of TET on HCA and comparison of protective validity between TET-HCA and UW solution during hypothermal preservation after perfusion in donor liver

目的 通过建立低温灌洗保存供肝2 h的实验模型,探讨低温灌洗保存条件下TET(汉防己甲素)对供肝的保护作用,分析TET对HCA液的优化效果,并与UW液的保护效果进行比较.方法 SD大鼠80只,随机平均分成4组.A组(TET乳酸林格组), B组(HCA液组),C组(TET-HCA液组),D组(UW液组).经实验处理后检测各组肝组织的高能磷酸化合物的含量及保养液中ALT及LDH值、并比较各组保存供肝的病理变化.结果 A组与B组各指标比较无明显差异(P>0.05);C组与B组各指标比较具有显著性差异(P 0.05).结论在低温灌洗保存供肝2 h条件下TET对供肝具有与 HCA液相当的保护作用;且对HCA液具有积极的优化作用; TET-HCA液低温灌洗并保存鼠肝2 h,对供肝的保护作用与UW液类同。