The hypophyseal substances of carp or bull-frog were administered to the hatchery rainbowtrout few weeks before their norml breeding season. Males treated with the hypophyses fell in maturation as early as one or a few days after the injection. (Exp. A) On the other hand females similarly treated, compared with those administered with the saline, showed no difference in the distribution of the dates when each ovulation was observed. (Exp. C) Upon the toutching perception from outside of the abdomen, however, the former showed some though slight maturation within a week after the injection, but the latter hardly. (Exp. B).