Harsh Stress Research Articles

Genome sequencing of Coryphaenoides yaquinae reveals convergent and lineage‐specific molecular evolution in deep‐sea adaptation

AbstractAbyssal (3501–6500 m) and hadal (>6500 m) fauna evolve under harsh abiotic stresses, characterized by high hydrostatic pressure, darkness and food shortage, providing unique opportunities to investigate mechanisms underlying environmental adaptation. Genomes of several hadal species have recently been reported. However, the genetic adaptation of deep sea species across a broad spectrum of ocean depths has yet to be thoroughly investigated, due to the challenges imposed by collecting the deep sea species. To elucidate the correlation between genetic innovation and vertical distribution, we generated a chromosome‐level genome assembly of the macrourids Coryphaenoides yaquinae, which is widely distributed in the abyssal/hadal zone ranging from 3655 to 7259 m in depth. Genomic comparisons among shallow, abyssal and hadal‐living species identified idiosyncratic and convergent genetic alterations underlying the extraordinary adaptations of deep‐sea species including light perception, circadian regulation, hydrostatic pressure and hunger tolerance. The deep‐sea fishes (Coryphaenoides Sp. and Pseudoliparis swirei) venturing into various ocean depths independently have undergone convergent amino acid substitutions in multiple proteins such as rhodopsin 1, pancreatic and duodenal homeobox 1 and melanocortin 4 receptor which are known or verified in zebrafish to be related with vision adaptation and energy expenditure. Convergent evolution events were also identified in heat shock protein 90 beta family member 1 and valosin‐containing protein genes known to be related to hydrostatic pressure adaptation specifically in fishes found around the hadal range. The uncovering of the molecular convergence among the deep‐sea species shed new light on the common genetic innovations required for deep‐sea adaptation by the fishes.

Kinetics and pathways of sub‐lithic microbial community (hypolithon) development

AbstractType I hypolithons are microbial communities dominated by Cyanobacteria. They adhere to the underside of semi‐translucent rocks in desert pavements, providing them with a refuge from the harsh abiotic stresses found on the desert soil surface. Despite their crucial role in soil nutrient cycling, our understanding of their growth rates and community development pathways remains limited. This study aimed to quantify the dynamics of hypolithon formation in the pavements of the Namib Desert. We established replicate arrays of sterile rock tiles with varying light transmission in two areas of the Namib Desert, each with different annual precipitation regimes. These were sampled annually over 7 years, and the samples were analysed using eDNA extraction and 16S rRNA gene amplicon sequencing. Our findings revealed that in the zone with higher precipitation, hypolithon formation became evident in semi‐translucent rocks 3 years after the arrays were set up. This coincided with a Cyanobacterial ‘bloom’ in the adherent microbial community in the third year. In contrast, no visible hypolithon formation was observed at the array set up in the hyper‐arid zone. This study provides the first quantitative evidence of the kinetics of hypolithon development in hot desert environments, suggesting that development rates are strongly influenced by precipitation regimes.

Hypermethylation of BMPR2 and TGF-β Promoter Regions in Tibetan Patients with High-Altitude Polycythemia at Extreme Altitude.

Although the expression of many genes is associated with adaptation to high-altitude hypoxic environments, the role of epigenetics in the response to this harsh environmental stress is currently unclear. We explored whether abnormal DNA promoter methylation levels of six genes, namely, ABCA1, SOD2, AKT1, VEGFR2, TGF-β, and BMPR2, affect the occurrence and development of high-altitude polycythemia (HAPC) in Tibetans. The methylation levels of HAPC and the control group of 130 Tibetans from very high altitudes (> 4500m) were examined using quantitative methylation-specific real-time PCR (QMSP). Depending on the type of data, the Pearson chi-square test, Wilcoxon rank-sum test, and Fisher exact test were used to assess the differences between the two groups. The correlation between the methylation levels of each gene and the hemoglobin content was explored using a linear mixed model. Our experiment revealed that the methylation levels of the TGF-β and BMPR2 genes differed significantly in the two groups (p < 0.05) and linear mixed model analysis showed that the correlation between the hemoglobin and methylation of ABCA1, TGF-β, and BMPR2 was statistically significant (p < 0.05). Our study suggests that levels of TGF-β and BMPR2 methylation are associated with the occurrence of HAPC in extreme-altitude Tibetan populations among 6 selected genes. Epigenetics may be involved in the pathogenesis of HAPC, and future experiments could combine gene and protein levels to verify the diagnostic value of TGF-β and BMPR2 methylation levels in HAPC.

AcrR1, a novel TetR/AcrR family repressor, mediates acid and antibiotic resistance and nisin biosynthesis in Lactococcus lactis F44

Lactococcus lactis, widely used in the manufacture of dairy products, encounters various environmental stresses both in natural habitats and during industrial processes. It has evolved intricate machinery of stress sensing and defense to survive harsh stress conditions. Here, we identified a novel TetR/AcrR family transcription regulator, designated AcrR1, to be a repressor for acid and antibiotic tolerance that was derepressed in the presence of vancomycin or under acid stress. The survival rates of acrR1 deletion strain ΔAcrR1 under acid and vancomycin stresses were about 28.7-fold (pH 3.0, HCl), 8.57-fold (pH 4.0, lactic acid) and 2.73-fold (300 ng/mL vancomycin) greater than that of original strain F44. We also demonstrated that ΔAcrR1 was better able to maintain intracellular pH homeostasis and had a lower affinity to vancomycin. No evident effects of AcrR1 deletion on the growth and morphology of strain F44 were observed. Subsequently, we characterized that the transcription level of genes associated with amino acids biosynthesis, carbohydrate transport and metabolism, multidrug resistance, and DNA repair proteins significantly upregulated in ΔAcrR1 using transcriptome analysis and quantitative reverse transcription-PCR assays. Additionally, AcrR1 could repress the transcription of the nisin post-translational modification gene, nisC, leading to a 16.3% increase in nisin yield after AcrR1 deletion. Our results not only refined the knowledge of the regulatory mechanism of TetR/AcrR family regulator in L. lactis, but presented a potential strategy to enhance industrial production of nisin.

DIV1: The master of awakening seeds in harsh salinity stress.

DIV1: The master of awakening seeds in harsh salinity stress.

Application of Finite Element Algorithms for Formula Racing Steering Wheels

The steering wheel of a Formula Student car has an important influence on the driving and handling stability of the car. The steering wheel of the racing car must have good mechanical and stable performance, taking into account lightweight and economic performance. Due to the small riding space of the car, the size of the steering wheel is not required to be too large. In this case, it is very difficult to meet its harsh stress state and control its vibration and noise. Based on the general requirements of the competition rules of the Formula Student racing competition, this paper constructs the geometric model of the steering wheel of the racing car by using the computer 3D design software CATIA. ANSYS software was used to conduct finite element analysis of the mechanical properties of the metal skeleton of the steering disc. Magnesium alloy, a light material with excellent mechanical properties, was selected, and combined with the actual working conditions of the steering disc, especially the force under extreme conditions, the computer simulation results showed that the force distribution and displacement of the steering disc met the design requirements. Considering that the noise and vibration of the steering wheel will have a great impact on the racer, the modal analysis of the steering wheel is carried out by using ANSYS software, and the analysis results show that there is no resonance or instability.

Nutrient deprivation induces mouse embryonic diapause mediated by Gator1 and Tsc2.

Embryonic diapause is a special reproductive phenomenon in mammals that helps embryos to survive various harsh stresses. However, the mechanisms of embryonic diapause induced by the maternal environment is still unclear. Here, we uncovered that nutrient deficiency in uterine fluid was essential for the induction of mouse embryonic diapause, shown by a decreased concentration of arginine, leucine, isoleucine, lysine, glucose and lactate in the uterine fluid of mice suffering from maternal starvation or ovariectomy. Moreover, mouse blastocysts cultured in a medium with reduced levels of these six components could mimic diapaused blastocysts. Our mechanistic study indicated that amino acid starvation-dependent Gator1 activation and carbohydrate starvation-dependent Tsc2 activation inhibited mTORC1, leading to induction of embryonic diapause. Our study elucidates the essential environmental factors in diapause induction.

Deterioration modes, mechanisms, and effects of flexible landfill facilities disposing hazardous waste

Dynamic performance and lifespan predictions are essential for understanding the whole life-cycle emissions and environmental impacts of landfills. However, there are knowledge gaps regarding the major failure modes and mechanisms in flexible hazardous waste landfills (FHWLs) and their potential effects on the service life of landfills. These issues limit the advancement of related research. In this study, focusing on flexible landfills, failure mode, mechanism, and effects analysis was conducted to identify the major failure modes, mechanisms, and impacts that may occur in landfills under extremely harsh chemical and stress conditions. The results indicate that there are approximately 33 major components and materials in landfills, corresponding to 35 potential failure modes and more than 60 underlying failure mechanisms. Notably, the failure of the drainage media in the drainage system is concerning and challenging to recover from. In contrast, the failure of the high-density polyethylene geomembrane (HDPE GMB) in the liner system (LS) is considered serious and is prone to occur, but it is also difficult to detect. Therefore, these two failure modes were identified as critical factors affecting the degradation of the performance and longevity of FHWLs. Finally, due to their high probability of occurrence and severe impacts, stress damage (physical damage) and oxidative aging were identified as the main degradation modes of HDPE GMB, the core material in FHWLs. This study provides key insights into performance prediction targets, failure modes, and failure mechanisms for further research on long-term landfill performance and life prediction. It prompts the government and stakeholders to re-examine the rationality behind the positioning of landfills, such as the assumptions of unlimited service and solid waste management endpoints. Therefore, it calls for a reassessment and optimization of the full life cycle emissions of landfills and related waste.

The role of transcriptional regulators in metal ion homeostasis of Mycobacterium tuberculosis.

Metal ions are essential trace elements for all living organisms and play critical catalytic, structural, and allosteric roles in many enzymes and transcription factors. Mycobacterium tuberculosis (MTB), as an intracellular pathogen, is usually found in host macrophages, where the bacterium can survive and replicate. One of the reasons why Tuberculosis (TB) is so difficult to eradicate is the continuous adaptation of its pathogen. It is capable of adapting to a wide range of harsh environmental stresses, including metal ion toxicity in the host macrophages. Altering the concentration of metal ions is the common host strategy to limit MTB replication and persistence. This review mainly focuses on transcriptional regulatory proteins in MTB that are involved in the regulation of metal ions such as iron, copper and zinc. The aim is to offer novel insights and strategies for screening targets for TB treatment, as well as for the development and design of new therapeutic interventions.

Aspergillus fumigatus escape mechanisms from its harsh survival environments.

Aspergillus fumigatus is a ubiquitous pathogenic mold and causes several diseases, including mycotoxicosis, allergic reactions, and systemic diseases (invasive aspergillosis), with high mortality rates. In its ecological niche, the fungus has evolved and mastered many reply strategies to resist and survive against negative threats, including harsh environmental stress and deficiency of essential nutrients from natural environments, immunity responses and drug treatments in host, and competition from symbiotic microorganisms. Hence, treating A. fumigatus infection is a growing challenge. In this review, we summarized A. fumigatus reply strategies and escape mechanisms and clarified the main competitive or symbiotic relationships between A. fumigatus, viruses, bacteria, or fungi in host microecology. Additionally, we discussed the contemporary drug repertoire used to treat A. fumigatus and the latest evidence of potential resistance mechanisms. This review provides valuable knowledge which will stimulate further investigations and clinical applications for treating and preventing A. fumigatus infections. KEY POINTS: • Harsh living environment was a great challenge for A. fumigatus survival. • A. fumigatus has evolved multiple strategies to escape host immune responses. • A. fumigatus withstands antifungal drugs via intrinsic escape mechanisms.

Optical evaluation of the surfaces of the CuAlBe samples after the test of resistance to harsh wear stresses in an explosive atmosphere

A material with both non-sparking and good mechanical properties based on CuBe-Al elements was proposed for anti-sparking materials intended for use in potentially explosive atmospheres (design and manufacture of technical equipment, tools and implements intended for use in potentially explosive atmospheres). The alloy was obtained using a classical induction furnace from Cu-Be master alloy and pure Al. Using hot-rolled bands (heated to 800 °C and rolled from ingots with 10 mm diameter to 2 mm thickness plates) experimental devices were made to perform laboratory tests in explosive mixtures for the acceptance of spark arresting materials intended for use in potentially explosive atmospheres. A hard wear process by forcefully applying some steel elements to the experimental CuAlBe alloy was carried out in an enclosure with an explosive atmosphere consisting of an atmosphere with 10% H2. The main purpose of the tests is to verify whether or not the tested materials produce mechanical sparks. They are considered non-sparking materials and are checked/tested accordingly. Their appearance was not observed in the first 16000 friction tests between the two materials. The tests left deep traces of wear on the surface of the experimental alloys, leading to damage to their surface. The wear marks were analyzed macroscopically by optical microscopy (LOM). The test response after 16 000 frictions was negative to explosion and the material can be used as non spark alloy.

Fabrication of dry S/O/W microcapsule and its probiotic protection against different stresses.

Encapsulation is commonly used to protect probiotics against harsh stresses. Thus, the fabrication of microcapsules with special structure is critical. In this work, microcapsules with the structure of S/O/W (solid-in-oil-in-water) emulsion were prepared for probiotics, with butterfat containing probiotics as the inner core and with whey protein isolate fibrils (WPIF) and antioxidants (epigallocatechin gallate, EGCG; glutathione, GSH) as the outer shell. Based on the high viscosity and good emulsifying ability of WPIF, dry well-dispersed microcapsules were successfully prepared via the stabilization of the butterfat emulsion during freeze-drying with 30-50 g L-1 WPIF. WPIF, WPIF + EGCG, and WPIF + GSH microcapsules with 50 g L-1 WPIF protected probiotics very well against different stresses and exhibited similar inactivation results, indicating that EGCG and GSH exerted neither harm or protection on probiotics. This significantly reduced the harmful effects of antioxidants on probiotics. Almost all the probiotics survived after pasteurization, which was critical for the use of probiotics in other foods. The inactivation values of probiotics in microcapsules were around 1 log in simulated gastric juice (SGJ), about 0.5 log in simulated intestinal juice (SIJ), and around 1 log after 40 days of ambient storage. Dry S/O/W microcapsule, with butterfat containing probiotics as the inner core and WPIF as the outer shell, significantly increased the resistance of probiotics to harsh environments. This work proposed a preparation method of dry S/O/W microcapsule with core/shell structure, which could be used in the encapsulation of probiotics and other bioactive ingredients.

Identifying and Monitoring Gardens in Urban Areas Using Aerial and Satellite Imagery

In dry regions, gardens and trees within the urban space are of considerable significance. These gardens are facing harsh weather conditions and environmental stresses; on the other hand, due to the high value of land in urban areas, they are constantly subject to destruction and land use change. Therefore, the identification and monitoring of gardens in urban areas in dry regions and their impact on the ecosystem are the aims of this study. The data utilized are aerial and Sentinel-2 images (2018–2022) for Yazd Township in Iran. Several satellite and aerial image fusion methods were employed and compared. The root mean square error (RMSE) of horizontal shortcut connections (HSC) and color normalization (CN) were the highest compared to other methods with values of 18.37 and 17.5, respectively, while the Ehlers method showed the highest accuracy with a RMSE value of 12.3. The normalized difference vegetation index (NDVI) was then calculated using the images with 15 cm spatial resolution retrieved from the fusion. Aerial images were classified by NDVI and digital surface model (DSM) using object-oriented methods. Different object-oriented classification methods were investigated, including support vector machine (SVM), Bayes, random forest (RF), and k-nearest neighbor (KNN). SVM showed the greatest accuracy with overall accuracy (OA) and kappa of 86.2 and 0.89, respectively, followed by RF with OA and kappa of 83.1 and 0.87, respectively. Separating the gardens using NDVI, DSM, and aerial images from 2018, the images were fused in 2022, and the current status of the gardens and associated changes were classified into completely dried, drying, acceptable, and desirable conditions. It was found that gardens with a small area were more prone to destruction, and 120 buildings were built in the existing gardens in the region during 2018–2022. Moreover, the monitoring of land surface temperature (LST) showed an increase of 14 °C in the areas that were changed from gardens to buildings.

Mechanically Robust Nanoporous Polyimide/Silica Aerogels for Thermal Superinsulation of Aircraft

Polyimide aerogels (PIAs) are thermally insulating materials that possess various advantages, such as exceptionally high temperature resistance and low thermal conductivity, which make them great potential candidate materials to be applied in the area of thermal protection. However, under harsh mechanical stresses, the macro- and microscopic structural stability of PIAs may be compromised due to shrinkage/collapse, leading to performance degradation. Herein, we propose a homogeneous organic/inorganic hybrid formation strategy for constructing nanoporous polyimide/silica aerogels (PIAs-A) with exceptional mechanical strength and ultralow thermal conductivity. The results obtained indicate that PIAs-A have an optimal three-dimensional nanoporous network structure with a pore size distribution mainly within the range of 10–20 nm. Monitoring the temperature evolution of the material’s cold surface showed that it remained at a low temperature of 37.5 °C even when the material was placed against a hot surface of 150 °C. The residual mechanical strengths of the aerogels remained at a superior level (with strength degradation being less than 9%) after exposure to ultralow and high-temperature atmospheres. Furthermore, compressive stress values at 3% strain exceeded previously reported values for PIAs by 625 and 733% at −50 and 100 °C, respectively. Meanwhile, our aerogels displayed flame resistance even when exposed to a heat source of approximately 1200 °C, possessed favorable hydrophobic properties, and maintained dimensional stability up to 504 °C. The robust mechanical and thermal insulation properties of PIAs-A make them a promising substitute material for thermal superinsulation in aircraft.

Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities.

Lichens have developed numerous adaptations to optimise their survival under harsh abiotic stress, colonise different substrates, and reach substantial population sizes and high coverage in ice-free Antarctic areas, benefiting from a symbiotic lifestyle. As lichen thalli represent consortia with an unknown number of participants, it is important to know about the accessory organisms and their relationships with various environmental conditions. To this end, we analysed lichen-associated communities from Himantormia lugubris, Placopsis antarctica, P. contortuplicata, and Ramalina terebrata, collected from soils with differing deglaciation times, using a metabarcoding approach. In general, many more Ascomycete taxa are associated with the investigated lichens compared to Basidiomycota. Given our sampling, a consistently higher number of lichen-associated eukaryotes are estimated to be present in areas with deglaciation times of longer than 5000 years compared to more recently deglaciated areas. Thus far, members of Dothideomycetes, Leotiomycetes, and Arthoniomycetes have been restricted to the Placopsis specimens from areas with deglaciation times longer than 5000 years. Striking differences between the associated organisms of R. terebrata and H. lugubris have also been discovered. Thus, a species-specific basidiomycete, Tremella, was revealed for R. terebrata, as was a member of Capnodiales for H. lugubris. Our study provides further understanding of the complex terricolous lichen-associated mycobiome using the metabarcoding approach. It also illustrates the necessity to extend our knowledge of complex lichen symbiosis and further improve the coverage of microbial eukaryotes in DNA barcode libraries, including more extended sampling.

Coordinated and Distinct Roles of Peptidoglycan Carboxypeptidases DacC and DacA in Cell Growth and Shape Maintenance under Stress Conditions.

Peptidoglycan (PG) is an essential bacterial architecture pivotal for shape maintenance and adaptation to osmotic stress. Although PG synthesis and modification are tightly regulated under harsh environmental stresses, few related mechanisms have been investigated. In this study, we aimed to investigate the coordinated and distinct roles of the PG dd-carboxypeptidases (DD-CPases) DacC and DacA in cell growth under alkaline and salt stresses and shape maintenance in Escherichia coli. We found that DacC is an alkaline DD-CPase, the enzyme activity and protein stability of which are significantly enhanced under alkaline stress. Both DacC and DacA were required for bacterial growth under alkaline stress, whereas only DacA was required for growth under salt stress. Under normal growth conditions, only DacA was necessary for cell shape maintenance, while under alkaline stress conditions, both DacA and DacC were necessary for cell shape maintenance, but their roles were distinct. Notably, all of these roles of DacC and DacA were independent of ld-transpeptidases, which are necessary for the formation of PG 3-3 cross-links and covalent bonds between PG and the outer membrane lipoprotein Lpp. Instead, DacC and DacA interacted with penicillin-binding proteins (PBPs)-dd-transpeptidases-mostly in a C-terminal domain-dependent manner, and these interactions were necessary for most of their roles. Collectively, our results demonstrate the coordinated and distinct novel roles of DD-CPases in bacterial growth and shape maintenance under stress conditions and provide novel insights into the cellular functions of DD-CPases associated with PBPs. IMPORTANCE Most bacteria have a peptidoglycan architecture for cell shape maintenance and protection against osmotic challenges. Peptidoglycan dd-carboxypeptidases control the amount of pentapeptide substrates, which are used in the formation of 4-3 cross-links by the peptidoglycan synthetic dd-transpeptidases, penicillin-binding proteins (PBPs). Seven dd-carboxypeptidases exist in Escherichia coli, but the physiological significance of their redundancy and their roles in peptidoglycan synthesis are poorly understood. Here, we showed that DacC is an alkaline dd-carboxypeptidase for which both protein stability and enzyme activity are significantly enhanced at high pH. Strikingly, dd-carboxypeptidases DacC and DacA physically interacted with PBPs, and these interactions were necessary for cell shape maintenance as well as growth under alkaline and salt stresses. Thus, cooperation between dd-carboxypeptidases and PBPs may allow E. coli to overcome various stresses and to maintain cell shape.

Molecular Characterization of Dehydrin in Azraq Saltbush among Related Atriplex Species

Atriplex spp. (saltbush) is known to survive extremely harsh environmental stresses such as salinity and drought. It mitigates such conditions based on specialized physiological and biochemical characteristics. Dehydrin genes (DHNs) are considered major players in this adaptation. In this study, a novel DHN gene from Azrak (Jordan) saltbush was characterized along with other Atriplex species from diverse habitats. Intronless DHN-expressed sequence tags (495-761 bp) were successfully cloned and sequenced. Saltbush dehydrins contain one S-segment followed by three K-segments: an arrangement called SK3-type. Two substantial insertions were detected including three copies of the K2-segemnet in A. canescens. New motif variants other than the six-serine standard were evident in the S-segment. AhaDHN1 (A. halimus) has a cysteine residue (SSCSSS), while AgaDHN1 (A. gardneri var. utahensis) has an isoleucine residue (SISSSS). In contrast to the conserved K1-segment, both the K2- and K3-segment showed several substitutions, particularly in AnuDHN1 (A. nummularia). In addition, a parsimony phylogenetic tree based on homologs from related genera was constructed. The phylogenetic tree resolved DHNs for all of the investigated Atriplex species in a superclade with an 85% bootstrap value. Nonetheless, the DHN isolated from Azraq saltbush was uniquely subclustred with a related genera Halimione portulacoides. The characterized DHNs revealed tremendous diversification among the Atriplex species, which opens a new venue for their functional analysis.

К вопросу о судьбе военнопленных из сталинградского «котла»

Introduction. The successful counter-offensive of the Soviet troops near Stalingrad marked the beginning of the mass capture of enemy soldiers. Only after the liquidation of the Stalingrad cauldron, more than 90 thousand people were taken prisoner. Methods and materials. Historical facts are researched on the basis of the principles of historicism and objectivity. The article uses problem-chronological and comparative-historical methods. The article is based on the archival and published documents of the NKVD Secretariat, the Main Directorate for Prisoners of War Central Office and NKVD Internees and the Joint Archival Fund: Institutions for Prisoners of War and Internees of the Volgograd Region. Analysis. The captured enemy soldiers were exhausted and weakened to the limit, poorly dressed, all with lice, many sick and non-transportable. At the same time, it was impossible to create normal conditions in the destroyed city in a short period to accommodate captured enemy soldiers. A month-long stay in the frontline led to the fact that only 30% of the prisoners of war could be taken to the rear camps. In the special hospitals for prisoners of war created in Stalingrad and the region, the mortality rate until the beginning of June 1943 amounted to 63% of those admitted for treatment. But most of the prisoners sent to the rear could not move the road or died in the first days upon arrival at the camps. Results. About 80 thousand prisoners of war from the Stalingrad cauldron died already in 1943. The main reason for the high mortality among them was a long stay in harsh winter conditions and stress because of being in the cauldron without regular food. The low readiness of the NKVD camps and special hospitals to receive prisoners of war also had an effect.

The Tardigrade damage suppressor protein Dsup promotes DNA damage in neurons

Tardigrades are microscopic invertebrates, which are capable of withstanding extreme environmental conditions, including high levels of radiation. A Tardigrade protein, Dsup (Damage Suppressor), protects the Tardigrade's DNA during harsh environmental stress and X-rays. When expressed in cancer cells, Dsup protects DNA from single- and double-strand breaks (DSBs) induced by radiation, increases survival of irradiated cells, and protects DNA from reactive oxygen species. These unusual properties of Dsup suggested that understanding how the protein functions may help in the design of small molecules that could protect humans during radiotherapy or space travel. Here, we investigated if Dsup is protective in cortical neurons cultured from rat embryos. We discovered that, in cortical neurons, the codon-optimized Dsup localizes to the nucleus and, surprisingly, promotes neurotoxicity, leading to neurodegeneration. Unexpectedly, we found that Dsup expression results in the formation of DNA DSBs in cultured neurons. With electron microscopy, we discovered that Dsup promotes chromatin condensation. Unlike Dsup's protective properties in cancerous cells, in neurons, Dsup promotes neurotoxicity, induces DNA damage, and rearranges chromatin. Neurons are sensitive to Dsup, and Dsup is a doubtful surrogate for DNA protection in neuronal cells.

Shear Fatigue Analysis of SAC-Bi Solder Joint Exposed to Varying Stress Cycling Conditions

Sn–Ag–Cu (SAC) solder alloys are frequently used in electronic assemblies, and their fatigue resistance under various stress cycles is a significant factor that influences the reliability of electronics. Three distinct SAC-based solder joints (SAC305, SAC-Q, and SAC-R) were examined under various stress amplitude cycling and ambient temperature conditions. The stress on individual solder joints alternates between mild stress (MS) and harsh stress (HS) until complete failure. The hysteresis loop was investigated and compared under various stress amplitude cycles. In addition, the morphology and microstructure of the solder joints were analyzed. The results revealed that SAC-Q, with high Bi and Ag content, demonstrated superior fatigue resistance compared with SAC305 and SAC-R under varying stress amplitude conditions. The inelastic work increased with each change between the MS and HS cycles. SAC-Q exhibited solid solution and precipitate hardening mechanisms through the presence of bismuth (Bi) and silver (Ag) in the tin (Sn) lattice. At the same time, SAC305 showed Ag3Sn precipitates hardening, whereas SAC-R, with no Ag, demonstrated solid solution hardening exclusively. Different failure mechanisms were observed depending on the alloy composition and strain rate. SAC305 and SAC-R exhibited ductile failure. However, SAC-Q experienced an interfacial fracture. Excessive Bi may lead to the embrittlement of solder joints and a change in the failure mechanisms during mechanical testing.