Objective To establish the diversification nursing education mode, provide standardized training for rotation nurses and evaluate its effects in rotation nurses training, so as to improve the integral level of nursing team. Methods A total of 36 rotation nurses trained in Department of Internal Medicine of the Fourth People′s Hospital of Nantong were selected as the research object from July 2011 to January 2016 by cluster sampling method. The standardized training was carried out by focusing on training excellent clinical teachers, unifying theory textbooks for new nurses, formulating standardized teaching and assessment plan, implementing the teaching of cases combined with theoretical knowledge, teaching nurses to set up noble values, structuring a harmonious team atmosphere. And then, the self-efficacy, core competence, theory, operation, clinical working ability and anxiety of rotation nurses were compared before and after training. Results The score of self-efficacy of rotation nurses after training (2.41±0.13) was higher than that before training (1.85±0.15) with a significant difference (t=19.002, P<0.01) . The score of core competence significantly increased from (121.90±5.07) before training to (145.67±7.57) after training (t=19.794, P<0.01) . The scores of theory, operational skill, clinical working ability significantly increased from (77.86±4.40) , (79.47±4.99) and (68.58±3.19) before training to (88.28±5.44) , (90.92±4.75) and (83.31±5.20) after training with significant differences (t=9.564, 10.732, 12.618; P<0.01) . The score of anxiety after training (30.94±4.85) was lower than that before training (41.75±12.11) with a significant difference (t=-7.401, P<0.01) . Conclusions The diversification nursing education can improve the comprehensive ability of rotation nurses by training excellent clinical teachers, unifying theory textbooks for new nurses, formulating standardized teaching and assessment plan and so on. It is worthy of popularization and application in clinical teaching. Key words: Nurses; Standardized training; Diversification nursing education; Rotate; Clinical teaching