This paper describes in-flight and wind tunnel research into laminar-turbulent transition. Measurements were carried out with a laminar wing glove for a glider (Twin II Grob G103), which could also be used in the large laminar wind tunnel at the Institute for Aerodynamics and Gasdynamics in Stuttgart. The central aspect of the investigation was the survey of the temporal–spatial development and propagation of natural as well as controlled generated waves. For the experiments performed, varied sensor arrays were used which allowed the two-dimensional acquisition of flow information on the glove (surface hot-wire and piezo foil sensors). Thus the amplification and the spatial distribution of the disturbances could be measured and compared in flight as well as in the wind tunnel, beginning with the very early linear amplification stage to the early non-linear stage of transition. For the investigation of controlled transition, multiple spanwise adjacent harmonic point sources were used which were operated independently.