Harmful Radiation Research Articles

An Updated Review on the Role of Phytoconstituents in Modulating Signalling Pathways to Combat Skin Ageing: Nature’s Own Weapons and Approaches

Abstract: Various geographical areas exhibit varying degrees of prevalence and severity of dermatological issues. The most commonly observed skin issues among adolescents during their growth period on a global scale encompass dry skin, dyspigmentation, wrinkles, fungal infections, as well as benign and malignant tumors. These conditions arise as a consequence of diminished functional capacity and heightened skin susceptibility. The primary manifestation of the whole process of skin ageing is its visual presentation, which encompasses changes in both the structure and function of the skin. The look and function of human skin exhibit particular variations as individuals age, representing a time-dependent phenomenon. This review article primarily examines the discussion surrounding the diverse phytoconstituents and their impact on signalling pathways in cellular metabolism, as well as their interaction with environmental factors and xenobiotic agents that contribute to skin aging. Ultraviolet (UV) light induces the rapid formation and subsequent accumulation of reactive oxygen species (ROS) within skin cells, hence accelerating oxidative stress and the ageing process of the skin. One effective approach to addressing age-related skin disorders entails the utilization of exogenous supplementation through the consumption of dietary antioxidants, as well as the application of antioxidant-based lotions to the skin prior to sun exposure. Several plant species include phenolic components, including ascorbic acid, ellagitannins, and carotenoids, which have the ability to protect the skin from harmful UV radiation, reduce inflammation and oxidative stress, and influence several survival signalling pathways. This comprehensive study elucidated multiple processes by which phytoconstituents exert their effects for intervention purposes. Additionally, it highlighted the ability of these phytoconstituents to modulate the NF-κB signalling pathway, MAPK signalling, Nrf2 signalling, and other pathways, hence demonstrating their potential anti-aging properties.

Bamboo-Based Carbon/Co/CoO Heterojunction Structures Based on a Multi-Layer Periodic Matrix Array Can Be Used for Efficient Electromagnetic Attenuation

With the popularization of wireless communication, radar, and electronic devices, the hidden harm of electromagnetic radiation is becoming increasingly serious. The design of green biomass carbon-based interface heterojunctions based on lightweight porous materials can effectively protect against electromagnetic radiation hazards. In this work, we constructed an anisotropic heterojunction interface with magnetic and dielectric coupling based on a honeycomb-like periodic matrix multi-layer array repeating unit. The removal of lignin components from bamboo through oxidation enriches the impregnation pores and uniform adsorption sites of the magnetic medium. Further, in situ pyrolysis promotes the formation of a large number of electric dipoles at the interface between the magnetic medium and dielectric coupling inside the periodic cell carbon skeleton, enhancing interface polarization and relaxation. Local carrier traps and uneven electromagnetic density enhance dielectric and hysteresis losses, resulting in excellent impedance matching. Therefore, the obtained bamboo-based carbon multiphase composite absorbent has satisfactory electromagnetic loss characteristics. At a thickness of 1.55 mm, the effective absorption bandwidth reaches 5.1 GHz, and the minimum reflection loss (RL) value reaches −54.7 dB. In addition, the far-field radar simulation results show that the sample has an excellent RCS (radar cross-section) reduction of 33.3 dB·m2. This work provides new directions for the diversified development of green biomass and the optimization of the design of magnetic and dielectric coupling in periodic array structures.

Nanofiberous composite with CuO-Ag0 supported on bamboo/lycra for protection against transmittable infectious diseases

The transmittable infectious diseases, detrimental environmental contaminants and harmful UV radiations remained constant threat to human civilization. Cold, pneumonia, tuberculosis bacteria, rubella virus, RSV and COVID19, then urban and industrial emissions, volcanic eruptions, pollen, aerosols on one hand and skin cancer, sunburn, premature ageing and skin tanning on the other hand are some of the examples of serious risks to health, safety and well-being. The WHO reported over 7million worldwide deaths due to hazardous air contaminants. Exploiting the tremendous features of nanofiberous structure and excellent microbiocidal properties of Ag0 and CuO nanoparticles, a sustainable and stable nanofiber based composite Bmb-nanoNP was developed. Utilization of biocompatible, biodegradable, nontoxic and environmental friendly polymers enabled the composite as sustainable source for protective clothing purposes. The microparticulate filtration of Bmb-nanoNP was 99.13 % and 96 % with and without air suction respectively. The microbiocidal affect against E. coli and S. aureus strains was excellent(5.9 % and 8.86 % cell viabilities respectively). Similarly, the Bmb-nanoNP showed calculated UV protection factor (UPF) of 7954 (50 + rating).These characteristics demonstrated potential candidacy of the Bmb-nanoNP composite for multi-functional safety clothing applications.

Monolithically integrated neuromorphic electronic skin for biomimetic radiation shielding.

Melanogenesis, a natural responsive mechanism of human skin to harmful radiation, is a self-triggered defensive neural activity safeguarding the body from radiation exposure in advance. With the increasing significance of radiation shielding in diverse medical health care and wearable applications, a biomimetic neuromorphic optoelectronic system with adaptive radiation shielding capability is often needed. Here, we demonstrate a transparent and flexible metal oxide-based photovoltaic neuromorphic defensive system. By using a monolithically integrated ultraflexible optoelectronic circuitry and electrochromic device, seamless neural processing for ultraviolet (UV) radiation shielding including history-based sensing, memorizing, risk recognition, and blocking can be realized with piling the entire signal chain into the flexible devices. The UV shielding capability of the system can be evaluated as autonomous blocking up to 97% of UV radiation from 5 to 90 watts per square meter in less than 16.9 seconds, demonstrating autonomously modulated sensitivity and response time corresponding to UV environmental conditions and supplied bias.

Food Polyphenols in Radiation-Related Diseases: The Roles and Possible Mechanisms.

As science and technology continue to evolve, the potential harm of radiation to the human body cannot be overlooked. Radiation has the capacity to inflict cellular and body-wide damage. Polyphenols are a group of naturally occurring compounds that are found in an array of plant foods. Scientific studies have demonstrated that these compounds possess noteworthy anti-radiation efficacy. Furthermore, they have been observed to be less toxic at higher doses. In the present review, we discussed the mechanisms of ionizing radiation damage and the progress in the research on the radiation resistance mechanism of polyphenol compounds, to provide guidance for the prevention and treatment of radiation related diseases. Food polyphenols can reduce the oxidative damage caused by ionizing radiation, clear free radicals, reduce DNA damage, regulate NF-KB, MAPK, JAK/STAT, Wnt and other signaling pathways, improve immune function, and have significant protective effects on radiation-induced inflammation, fibrosis, cancer and other aspects. In addition, it also has significant dual effects on radiation sensitization and radiation protection. Food polyphenols come from a wide range of sources, are abundant in daily food, and have no toxic side effects, demonstrating that food polyphenols have great advantages in preventing and treating radiation-related diseases.

Cinnamon and Eucalyptus Extracts: A Promising Natural Approach for Durable Mosquito-Repellent Fabrics with Multifunctionality.

Mosquitoes are highly important carriers of diseases, such as malaria, dengue, chikungunya, and various other life-threatening illnesses. Traditionally, many chemicals, such as plant extracts, oils, and smoke, have been employed for the purpose of repelling mosquitoes. Various plants possess essential oils and chemicals that have been proven to be good insect repellents and are commonly regarded as weeds. The present study focused on the development of eco-friendly, nonhazardous mosquito-repellent fabrics using cinnamon and eucalyptus extracts. First, eucalyptus and cinnamon extracts were produced separately using ethanol and water as solvents with and without heating. Forty-eight different fabric samples were prepared by applying these extracts at three levels of process application temperature. A steam dye bath sampling machine was utilized to execute the extraction application process on fabric samples. The mosquito repellency performances of all of the samples were evaluated using the cage test method. The cage test revealed that all of the samples of eucalyptus and cinnamon extract-applied fabrics showed mosquito repellency performance at some level. However, the fabric samples treated with the heated extract of eucalyptus ethanol (EE-H) at 60 °C showed the best results in terms of mosquito repellency (85.56%) among all combinations. In addition to repellency, the impact of washing durability, UV shielding, and antibacterial performance was also evaluated. This research demonstrated a new method for creating a fabric that repels mosquitoes and has effective antibacterial properties as well as promising ultraviolet protection factor (UPF) rating. This fabric protects the wearer from the significant health risks posed by mosquitoes and harmful UV radiation while also maintaining its cleanliness. Moreover, the utilization and implementation of plant-derived coatings on textiles contribute to the advancement of sustainable methods (SDG 9 and SDG 12) in the chemical processing industry of textiles, ultimately leading to a reduction in their environmental footprint.

Aerospace Medicine – An Evolving Field to Mitigate and Treat Organ Dysfunction Partly Caused by Premature Aging in Low Microgravity

Space medicine is a branch of aerospace medicine, which is a crucial field, albeit with minimal research. Research at the International Space Station and other endeavors has provided insights into how loss of gravity could affect the physiological functions of humans. Organs such as the heart could begin to deteriorate with lack of resistance from the planet. The musculoskeletal system, which is the anatomical backbone, may struggle to support the individual’s structure. The cardiovascular system will be inefficient with respect to circulation, leading to other organ dysfunction. Radiation in space, also known as cosmic radiation, is caused by many different celestial bodies such as stars, sun, supernovae, and black holes. The Earth’s magnetic field is a crucial component to protect humans from harmful radiation. However, once humans venture beyond the protective confines, the body becomes exposed to dangerous elements that pose a significant threat to safety. Living in microgravity also has detrimental effects on organisms’ brains. These include but are not limited to sustained effects on circadian rhythm, emotional dysregulation, and cognitive dysfunction. This article discusses the threat of premature aging during long- and short-term times in space due to high risks associated with space travel. Improvement of clinical intervention is hindered by a lack of research and development, which is needed to fundamentally address these risks.

Environmental Ethics in Islāmic Perspective: Relevance and Application

God has created all living and non-living things with a distinctive purpose. As the Sustainer of all, He has also arranged for each living organism a desirable environment that provides sustainable items. So, each life requires a sound environment for sustainability. Ecological studies help us understand organisms’ relationship to the environment around them. As God’s highest creation, humans have a close relationship with Nature to maintain and sustain their lives in vibrant caravans. The philosophical interpretations do accept the intimate relationship between humanity and Nature. However, our lifestyle is based on the rich consumption of resources, the development of new technologies and sciences for processing natural resources, burning fossil fuels, and discharging harmful radiation, which has alienated Nature from humanity. We can also point out other causes responsible for a radical break between human society and Nature. The Qur’ān and the Sunnah of the Prophet of Islām are the primary sources of all arts and thoughts. They may lead us to develop an Islāmic perspective on ecological crises and help us solve them. It is attempted here to understand some of the vital environmental phenomena in the light of these sources of Islām. There is intrinsic goodness, beauty, harmony and orderliness in the creation of God; everything in it, the sky and earth, including the sun, moon, stars, water, air, land and a thousand other things have been created for the benefit of man. The Creator created the universe with a Divine trust, which he had accepted and blessed to live in. He thanks God for all bestowed gifts practical in his entire gamut of human emotional life. From the Islāmic perspective, environmental ethics has become intrinsically relevant to dealing with all issues related to ecological crises. In the present paper, we are attempting to briefly discuss the Islāmic perspective on environmental ethics with particular reference to the Qur’ān and the traditions of Prophet Muhammad. It is assumed that the Islāmic framework on the said topic can also pave the way to bring out its relevance and application in crucial times. Philosophy and Progress, Vol#73-74; No#1-2; Jan-Dec 2023 P 1-20

Exploring Patient Preferences for Information About CT Radiation Exposure: Bridging the Gap Between Patient Preference and Physician Practice.

CT scan utilizes ionizing radiation poses a danger to the patient's health. Thus, telling the patient about ionizing radiation would be critical in promoting shared decision-making and improving patient-doctor communication. However, few studies have examined this topic broadly. The study was conducted to identify the frequency of physicians informing patients about the radiation risk before ordering a CT scan, as well as to examine the association between patients' demographic characteristics and their awareness of the radiation risks associated with CT scans. A cross-sectional study was conducted among 387 patients who had undergone CT scans at a tertiary hospital in Riyadh, Saudi Arabia. Data were collected via phone interviews using a structured questionnaire. Chi-squared tests were employed to assess associations between patients' demographic characteristics and their awareness of CT scan radiation risks. When examining knowledge, 58% of patients knew that CT involves harmful radiation. This knowledge was significantly associated with higher education level and previous experience with CT scans. Regarding doctors' practice of providing information to patients about the scan, 344 (88.9%) patients indicated that their doctor had explained to them why they needed the scan. Only 28 (7.2%) patients stated that their doctor had mentioned the amount of radiation, and 74 (19.1%) patients indicated that doctors mentioned the risks associated with the radiation of the scan. Almost all patients (96.9%) preferred to be told about why they needed a CT scan. The vast majority of patients who underwent CT scans did not receive enough information about the harm of the scans. However, most of them preferred to know about this harm.

Radiation shielding calculation for interventional radiology: An updated workload survey using a dose monitoring software

Shielding design is an essential aspect of radiation protection. It is necessary to ensure that barriers safeguard workers, patients, the general public, and the environment from the harmful radiation emitted by X-ray machines. The National Council on Radiation Protection and Measurements (NCRP) 147 method is widely accepted within the radiation protection experts’ (RPEs) community for structural shielding design for medical X-ray imaging facilities. However, these indications are based on data collected in 1996. In recent years, interventional radiology procedures have seen significant developments. Therefore, it is important to evaluate whether updating the data on workload in the different specialities is necessary.We extracted all interventional radiology exposure data parameters from three angiographs from two vendors using dose monitoring software for 3066 procedures and 214,697 individual exposures. The workload distribution as a function of the kVp for five interventional rooms was calculated by summing all exposures and then normalising them by the number of patients.Analysing this data, we obtained new transmission curves through lead, concrete and gypsum wallboard, finding the parameters (α, β, and γ) in the Archer equation for the secondary radiation. Finally, our aim was to share an example of shielding calculations for haemodynamics and neuroangiography rooms to illustrate the impact of updated transmission data.

Effects of UV-B and UV-C Spectrum Supplementation on the Antioxidant Properties and Photosynthetic Activity of Lettuce Cultivars.

Indoor farming systems enable plant production in precisely controlled environments. However, implementing stable growth conditions and the absence of stress stimulants can weaken plants' defense responses and limit the accumulation of bioactive, health-beneficial phytochemicals. A potential solution is the controlled application of stressors, such as supplemental ultraviolet (UV) light. To this end, we analyzed the efficiency of short-term pre-harvest supplementation of the red-green-blue (RGB, LED) spectrum with ultraviolet B (UV-B) or C (UV-C) light to boost phytochemical synthesis. Additionally, given the biological harm of UV radiation due to high-energy photons, we monitored plants' photosynthetic activity during treatment and their morphology as well as sensory attributes after the treatment. Our analyses showed that UV-B radiation did not negatively impact photosynthetic activity while significantly increasing the overall antioxidant potential of lettuce through enhanced levels of secondary metabolites (total phenolics, flavonoids, anthocyanins), carotenoids, and ascorbic acid. On the contrary, UV-C radiation-induced anthocyanin accumulation in the green leaf cultivar significantly harmed the photosynthetic apparatus and limited plant growth. Taken together, we showed that short-term UV-B light supplementation is an efficient method for lettuce biofortification with healthy phytochemicals, while UV-C treatment is not recommended due to the negative impact on the quality (morphology, sensory properties) of the obtained leafy products. These results are crucial for understanding the potential of UV light supplementation for producing functional plants.

Investigation of radiation knowledge and awareness among healthcare professionals in Mogadishu, Somalia: a single-center survey study

Objective: The aim of this study was to evaluate the radiation knowledge and awareness levels of healthcare workers working in a single center in Mogadishu, Somalia, in terms of age, gender and education levels of the participants. Materials and methods: The research involved 274 healthcare professionals, comprising volunteer physician and other healthcare personnel working at the Somali-Turkey Recep Tayyip Erdoğan Training and Research Hospital in Mogadishu. The face-to-face questionnaire consists of 13 questions covering radiation-sensitive or resistant tissues, radiation status of medical imaging devices, biological effects of radiation, ionizing and non-ionizing radiation symbols and radiation protection principles. The study was limited to the health personnel in the hospital. The survey had 274 participants, comprising 126 (46%) women and 148 (54%) men. Of these, 82 (29.9%) were physicians and 192 (70.1%) were other healthcare professionals. Results: It was found that health workers under the age of thirty demonstrated a significantly higher level of knowledge about radiation protection principles than their counterparts over the age of thirty (p = 0.003). Similarly, it was observed that the level of awareness regarding the extent of knowledge about radiation protection was higher in health workers under thirty years of age than in those over thirty years of age (p: 0.001). It was found to be statistically significant that males were more aware of the potential harms of radiation than females (p: 0.004). It was found to be statistically significant that men were more aware of the radiation content of mammography and X-ray imaging methods than women (p: 0.002 and p: 0.029, respectively). Conclusions: To enhance the number of healthcare professionals in Mogadishu, Somalia, who are aware of the risks associated with radiation and the methods for its mitigation, it was postulated that a comprehensive radiation information and awareness training program could be beneficial.

Challenges in illumination analysis and design for Martian surface-level habitation

It is needless to say that travel to and settlement on Mars are associated with extreme levels of scientific and engineering issues. This will only be amplified with the long-term duration of the mission, not only due to scarcity of resources, but also as the psychological aspects of the dynamics among the crew increase drastically. It should be emphasized that this is a scientific crew, who have undergone high levels of confinement during space travel to Mars, O (102 Earth days), are living in semi-solitude and partial confinement conditions for durations of O (103 Earth days), and even at the nominal termination of the mission, foresee a high-risk and arduous travel time of O (102 Earth days) back to the Earth. The mental weight of the described mission with its slow pace and tardy episodes, puts the crew under severe psychological issues. Minimal and conservative design of spaces, lack of constant access to the exterior, and social solitude are among major factors contributing to the psychological well-being of the crew. Furthermore, the overall lower levels of natural light, accompanied by the minimum possible area of transparent facades, protecting the crew from harmful radiations and cold exterior, burden the mental conditions of the crew even more. Given the limited availability of data from the surface of Mars, study of the effects linked to the lighting and illumination design of the habitats is challenging. The current manuscript hopes to shed light on the illumination and lighting design and simulation procedure, required data, assumptions, and final results for the surface-level habitats on Mars.•Mars / Sub orbital configuration allows for limited natural lighting, however, upon site-specific analysis, it might be considerable as a base passive source.•Current simulation tools are design based on Earth-bound design requirements. These need to be re-oriented to match available planetary data.

Externally supplied ascorbic acid moderates detrimental effects of UV-C exposure in cyanobacteria.

The defensive role performed by exogenously supplied ascorbic acid in the cyanobacterium Nostoc muscorum Meg1 against damages produced by UV-C radiation exposure was assessed in this study. Exposure to UV-C (24mJ/cm2) significantly enhanced reactive oxygen species (ROS) (50%) along with peroxidation of lipids (21%) and protein oxidation (22%) in the organism. But, addition of 0.5mM ascorbic acid prior to UV-C exposure showed reduction in ROS production (1.7%) and damages to lipids and proteins (1.5 and 2%, respectively). Light and transmission electron microscopic studies revealed that ascorbic acid not only protected filament breakage but also restricted severe ultrastructural changes and cellular damages in the organism. Although the growth of the organism was repressed up to 9% under UV-C treatment within 15days, a pre-treatment with ascorbic acid led to growth enhancement by 42% in the same period. Various growth parameters such as photo-absorbing pigments (phycoerythrin, phycocyanin, allophycocyanin, chlorophyll a, and carotenoids), water splitting complex (WSC), D1 protein, RuBisCO, glutamine synthetase and nitrogenase activities in the UV-C treated organism were seen to be relatively intact in the presence of ascorbic acid. Thus, a detailed analysis undertaken in the present study was able to demonstrate that ascorbic acid not only act as first responder against harmful UV-C radiation by down-regulating ROS production, it also accelerated the growth performance in the organism in the post UV-C incubation period as an immediate response to an adverse experience presented in the form of UV-C radiation exposure.

Green Nanoengineered Fabrics: Waste-Derived Polyphenol-Zinc@ Silica Core-Shell Reactive Janus Nanoparticles for Functional Fabrics.

Fabricating Janus nanoparticle-functionalized fabrics with UV protection, strength enhancement, self-cleaning properties, and wash durability, with a biocompatible nature, is crucial in modern functional fabrics engineering. Particularly, tailoring multifunctional nanoparticles capable of exhibiting several distinct properties, utilizing low-cost raw materials, and adhering to green chemistry principles is pivotal. A fabrication strategy for developing multifunctional reactive Janus nanoparticles, utilizing waste-derived natural polyphenol (quercetin-3-glucuronide, myricetin-3-galactoside, gossypin, phlorizin, kaempferol, myricetin-3-arabinoside)-integrated zinc-silica core-shell Janus nanoparticles with UV protection, strength enhancement, and self-cleaning properties, is proposed. Polyphenols were utilized as sustainable precursors for synthesizing zinc-polyphenol complexes, which were then encapsulated within a silica shell to form a core-shell structure. Furthermore, Janus particles were created by introducing a bifunctional layer with half amine/carboxylic acid and half methyl terminals, imparting reactive hydrophilic and hydrophobic properties. Janus-coated textiles and leather exhibited significant attenuation of harmful UV radiation, with water contact angle measurements confirming improved water repellency. The coexistence of natural phenols and bifunctional groups within a material bolstered textile strength, fostering superior adhesion and markedly enhancing wash durability. This eco-friendly approach, utilizing waste-derived materials, presents a promising solution for sustainable textile engineering with enhanced performance in UV protection and water resistance, thereby contributing to the advancement of green nanotechnology in textile applications.

Biosynthesis of secondary metabolites in aromatic and medicinal plants in response to abiotic stresses: A review

Climate change has massive consequences on non-living factors in the environment, resulting in irregular precipitation, fluctuating atmospheric temperature, and variations in humidity. These changes cause biotic and abiotic stresses; plants must have defense mechanisms to survive. Therefore, plants divert some synthesized energy towards producing numerous plant secondary metabolites (PSMs), viz., flavonoids, alkaloids, and essential oils. These compounds act as protections for the plants, helping them to survive under stressful conditions. Medicinal and aromatic plants (MAPs) are sessile organisms that are not immune to harmful consequences of various abiotic stresses in which the PSMs have an important role in acting against the adverse effects. In this regard, the MAPs have a coherent defense mechanism for abiotic stresses. The secondary metabolites produced by these plants are useful as medicines and aromatic products for humans. However, not all stresses produce high secondary metabolites, as their production is highly specific to certain stresses. This review provides a comprehensive understanding of secondary metabolite production under various stressful conditions, including extreme temperature, drought, water logging, salinity, harmful radiation, elevated levels of ozone and CO2, heavy metals, and agrochemicals on MAPs. Additionally, the production of these compounds can be modified by subjecting plants to various stressors. Many authors have reported on PSMs in MAPs, which need to be well documented and exploited for humankind.

Know Your Sunscreen

Ultraviolet(UV) radiation from the sun has significant adverse effects on the skin, including sunburns, premature aging (photoaging), tanning, and many skin cancers. Sunscreens protect from harmful UV radiation. This article delves into the fundamentals of solar radiation, focusing on the UV spectrum, its effect on the skin, various types of sunscreens, and their mechanism of action to offer protection from harmful rays. UV exposure depends on factors like geographic location, altitude, time of day, season, and surface reflectivity. Sunscreen efficacy is measured by the Sun Protection Factor (SPF), which indicates how well a sunscreen can protect against UVB-induced sunburn. SPF ratings of 15, 30, and 50+ correspond to 93%, 96%, and 98% UVB blockage, respectively. In contrast, UVA protection lacks a standardized measure, with various in vitro and in vivo indices used globally. Sunscreens are categorized as organic (chemical) or inorganic (physical), each with distinct mechanisms and properties. Proper application of sunscreen, involving adequate coverage and reapplication, maximizes its protective benefits, significantly reducing the risk of UV-induced skin damage and cancers. Despite minor side effects like potential allergies, the advantages of sunscreen use, including prevention of sunburn, tanning, premature aging, and skin cancers, far outweigh the drawbacks. UV radiation from the sun poses significant risks to skin health. Understanding the nature and effects of UV radiation is crucial in appreciating the importance of protective measures such as sunscreen. The role of sunscreens in providing comprehensive protection cannot be overstated.

Cyclo(l-Pro-l-Tyr) Isolated from the Human Skin Commensal Corynebacterium tuberculostearicum Inhibits Tyrosinase.

Melanin is produced by melanocytes to protect human skin from harmful ultraviolet radiation. During skin cell renewal, melanin and dead skin cells are disposed of. However, prolonged exposure to ultraviolet rays or aging can disturb this cycle, leading to skin hyperpigmentation due to melanin accumulation. Tyrosinase is a crucial enzyme involved in melanin biosynthesis. Although various compounds, including tyrosine inhibitors, that counteract melanin accumulation have been reported, some, such as hydroquinone, are toxic and can cause vitiligo. Meanwhile, the skin is the largest organ and the outermost layer of the immune system, containing a diverse range of bacteria that produce low-toxicity compounds. In the current study, we aim to identify metabolites produced by skin microbiota that inhibit tyrosinase. Specifically, mushroom tyrosinase served as the study model. Following commensal skin bacteria screening, Corynebacterium tuberculostearicum was found to inhibit tyrosinase activity. The active compound was cyclo(l-Pro-l-Tyr); commercially available cyclo(l-Pro-l-Tyr) also exhibited inhibitory activity. Docking simulations suggested that cyclo(l-Pro-l-Tyr) binds to the substrate-binding site of mushroom tyrosinase, obstructing the substrate pocket and preventing its activity. Hence, cyclo(l-Pro-l-Tyr) might have potential applications as a cosmetic agent and food additive.

Model‐Based Systems Engineering Approach for Designing an Artificial Magnetic Field Generator System for Spacecraft Radiation Protection

AbstractThe hazardous environment of space, dominated by cosmic and solar radiation, poses a significant threat to spacecraft and their occupants. Traditional radiation shielding methods, like passive materials, have limitations in weight and effectiveness. An artificial magnetic field generator system emerges as a promising solution to replicate Earth's magnetosphere, providing a protective magnetic shield against harmful radiation. (Dick, Launius, 2007) This paper presents a Model‐Based Systems Engineering (MBSE) approach to the holistic modeling and design of such a system.Utilizing the MBSE approach, this study models the system's components, their interactions, and the offered services, incorporating Radiation Monitoring, Magnetic Shielding, Power Management, System Health & Diagnostics, and Crew Communication services. The conceptual data model captures key entities and their relationships, ensuring a coherent integration of the system's parts. The activity diagram illustrates the operational flow, providing clarity on the system's dynamic behavior under varying radiation conditions and power reserves. A services taxonomy is developed to hierarchically categorize and ensure the comprehensive functionality of the system.The application of MBSE methodology provides numerous advantages, including a unified visualization of the system's complexities, enhanced stakeholder communication, and a streamlined validation and verification process. Furthermore, the flexibility inherent in the MBSE approach ensures that the system can be easily updated or scaled based on advancements in technology or changing mission requirements. (ECLIPSE Suite, 2022) The MBSE approach's application to the design of an artificial magnetic field generator system for spacecraft presents a robust and systematic method to ensure optimal performance, safety, and adaptability in the perilous realm of space.

Combined use of versatile peroxidase and aryl alcohol oxidase of Pleurotus eryngii to decolorize melanin on the skin

Melanin plays an important role in protecting skin cells from harmful UV radiation, but its uneven pigmentation in the skin sometimes demands cosmetic resolution. As a safe and effective way of evening the skin tone, enzymatic decolorization of melanin at the stratum corneum has been proposed. In this regard, the use of lignin peroxidase, in the presence of hydrogen peroxide and veratryl alcohol, an electron mediator, has been explored. Here, we first show that versatile peroxidase (VP) and aryl alcohol oxidase (AAO) purified from the Pleurotus eryngii liquid culture can be used to decolorize melanin without exogenous hydrogen peroxide. This resembles the oxidative degradation pathway of lignin in nature; AAO generates hydrogen peroxide from veratryl alcohol, and VP utilizes generated hydrogen peroxide while using veratryl alcohol as a mediator. We further explored the use of POX_Pe, the crude peroxidase preparation of the Pleurotus eryngii liquid culture, for melanin decolorization because of its better stability. Using POX_Pe and veratryl alcohol, over 60 % of melanin decolorization was obtained in 1 h in the absence of exogenous hydrogen peroxide addition. Furthermore, POX_Pe could decolorize melanin in a 3D human pigmented epidermis model, demonstrating its possible applications in cosmetics.