Harmful Food Research Articles

Associations of various healthy dietary patterns with biological age acceleration and the mediating role of gut microbiota: results from the China Multi-Ethnic Cohort study.

To investigate the associations between dietary patterns and biological aging, identify the most recommended dietary pattern for coping with biological aging and explore the potential mediating role of gut microbiota in less-developed ethnic minority regions (LEMRs). This prospective cohort study included 8288 participants aged 30-79 years from the China Multi-Ethnic Cohort study (CMEC). Anthropometric measurements and clinical biomarkers were utilized to construct biological age based on Klemera and Doubal's method (KDM-BA) and KDM-BA acceleration (KDM-AA). Dietary information was obtained through the baseline food frequency questionnaire (FFQ). Six dietary patterns were constructed: plant-based diet index (PDI), healthful plant-based diet index (hPDI), unhealthful plant-based diet index (uPDI), healthy diet score (HDS), Dietary Approaches to Stop Hypertension (DASH), and alternative Mediterranean diets (aMED). Follow-up adjusted for baseline analysis were employed to assess the associations between dietary patterns and KDM-AA. Additionally, quantile G-computation was utilized to evaluate the significant beneficial and harmful food groups. In the subsample of 764 participants with gut microbiota data obtained through 16S rRNA gene sequencing, we used causal mediation model to explore the mediating role of gut microbiota in the associations between dietary patterns and KDM-AA. The results showed that all dietary patterns were associated with KDM-AA. Transitioning from non-compliance to compliance, DASH exhibited the strongest negative association with KDM-AA [β = -0.91, 95%CI (-1.19, -0.63)]. The component analyses revealed that tea and soybean products were the significant beneficial food groups, while salt, preserved vegetables, red and processed meats were identified as the major harmful food groups. In mediation analysis, the decreased abundance of Synergistetes phylum and Pyramidobacter genus possibly mediated the negative associations between plant-based diets and KDM-AA (5.61%-9.19%). Overall, healthy dietary patterns, especially DASH, are negatively associated with biological aging in LEMRs. The Synergistetes and Pyramidobacter may mediate the associations between plant-based diets and biological aging. Developing appropriate strategies may promote healthy aging in LEMRs.

Method validation for the detection of formaldehydein fish using ultra performance liquid chromatography

Formaldehyde, the simplest aldehyde, tops the list of illegal and harmful food contaminants. Recently, formaldehyde has been used indiscriminately to preserve the freshness and quality of fish. Numerous incidents of formalin (a solution of formaldehyde) misuse during the storage and transportation of fish have been reported in several parts of India. In this context, it is pertinent to study formalin contamination in fish and shellfish. Protocol for detection of free and bound formaldehyde by ultra-performance liquid chromatography (UPLC) was attempted in the present study. Formaldehyde peak was observed in the expected retentiontime and standardisation of the protocol for formaldehyde determination was then carried out. UPLC method was developed and validated for specificity, repeatability, accuracy, and linearity. The average correlation coefficient (R2) was 0.99. The recovery rates of free and bound formaldehyde were 90.83 to 116.43% and 88.76 to 132.98%, respectively. The limit of detection (LOD) was found to be 0.5 ppm. The study investigated free and bound formaldehyde levels in commonly traded fish species across five major fish markets inTamil Nadu, India. Sphyraena barracuda had the highest recorded concentrations of free and bound formaldehyde at 9.8 and 6.45 mg kg-1, respectively, while Lethrinus lentjan exhibited the lowest concentrations at 0.8 mg kg-1 for free formaldehyde and 0.5 mg kg-1 for bound formaldehyde. Keywords: Formalin abuse, Limit of detection, Method validation, Retention time UPLC

Adverse Food Reactions: Physiological and Ecological Perspectives.

While food is essential for survival, it can also cause a variety of harmful effects, ranging from intolerance to specific nutrients to celiac disease and food allergies. In addition to nutrients, foods contain myriads of substances that can have either beneficial or detrimental effects on the animals consuming them. Consequently, all animals evolved defense mechanisms that protect them from harmful food components. These "antitoxin" defenses have some parallels with antimicrobial defenses and operate at a cost to the animal's fitness. These costs outweigh benefits when defense responses are exaggerated or mistargeted, resulting in adverse reactions to foods. Additionally, pathological effects of foods can stem from insufficient defenses, due to unabated toxicity of harmful food components. We discuss the structure of antitoxin defenses and how their failures can lead to a variety of adverse food reactions.

Pyridaben inhibits cell cycle progression and delays early embryonic development in zebrafish (Danio rerio)

Pyridaben is a broad-spectrum, contact-killing acaricide that can be used to control a variety of harmful food and plant mites. Pyridaben displays cardiotoxicity and liver toxicity toward fish, but the effects on fish embryonic development have not been characterized. We exposed early zebrafish embryos to 20, 30, and 40 μg/L concentrations of pyridaben. The exposure caused developmental abnormalities, including delayed embryonic shield formation, yolk sac resorption, decreases in body length, reduced pigmentation, and delays in hatching. Pyridaben caused a significant increase in the transcription level of the endoderm marker foxa2, but the transcription levels of the ectoderm development marker foxb1a and the mesoderm development marker snaila were not significantly altered. The transcription levels of the genes SOX17 in early embryos were significantly reduced. After exposure to pyridaben, catalase (CAT) activity and glutathione (GSH) content were increased, and cyclin D1, that is involved in early embryonic development, was abnormally expressed. This study shows that pyridaben causes anomalous development in zebrafish embryos by interfering with the cell cycle order of early embryonic development and inducing excessive oxidative stress. Colivelin, an agonist of the STAT3 signaling pathway, acted as a salvage drug to restore the cell cycle order during embryonic development following exposure to pyridaben. Thus, the toxic effects may be caused by pyridaben’s regulation of the STAT3 signaling pathway.

Hubungan Pengetauan Siswa terhadap Ketaatan Perilaku Konsumsi Jajanan Sehat di SDN 03 Sumber Salak Ledokombo Jember

School children are susceptible to various snacks sold both at school and outside school. Lack of attention to the consumption of healthy snacks, resulting in the impact of decreased health, ranging from poisoning due to unhygienic storage and presentation processes to the risk of cancer due to the use of harmful food additives (Anak et al., 2019). This study aims to determine the relationship between students' knowledge of the observance of healthy snacks consumption behavior in sdn 03 source salak ledokombo jember, This type of research is a quantitative descriptive research, the sample in this study is students with a total of 40 students. Two variables, namely the relationship between students' knowledge of the observance of healthy snack consumption behavior in sdn 03 source salak ledokombo jember, the instrument used is using a questionnaire, based on table 4.6, the results obtained are categorized as good (22.8%) as many as 29 students, categorized as moderate (7.2%) as many as 11 students and categorized as less none. Based on the results of research that has been conducted by researchers regarding the relationship between students' knowledge of eating healthy snacks, it can be concluded as follows: the relationship between students' knowledge of eating healthy snacks is categorized as good there are 29 students (22.8%) and categorized as moderate as 11 students (7.2%).

Review on novel approaches for controlling aflatoxin B1: Harnessing nature's defenses against food toxins

Aflatoxin B1 is the most harmful food toxin for humans and animals. According to the International Agency for Research on Cancer, it is ranked as one of the most carcinogenic substances in the world, directly connected to hepatocarcinoma in both humans and animals. Aflatoxin B1 is also involved in the occurrence of pathologies, and aflatoxicoses. Aflatoxin B1 can be produced by fungi from the genus Aspergillus section Flavi in a variety of foods during storage. Once aflatoxins are present, food processing cannot eradicate them. The damage and lack of complete elimination of aflatoxins from foodstuffs make them the most monitored and controlled substances in the world. Given the high-risk of Aflatoxin B1 on both animal and human health, it is important to study their worldwide distribution and existing control methods through a systematic review. Articles published from 1945 to 2022 found on several databases as Scopus, PubMed, Dimensions, Google Scholar and an extraction sieve was used to select the relevant articles. Of the two hundred and twenty-eight (228) French and English scientific articles on aflatoxins identified, forty of the most relevant original articles were selected for inclusion in this review following a rigorous selection process. Several genes are involved in the synthesis of aflatoxin B1. Moreover, certain environmental conditions, in particular oxidative stress are propitious for fungus by over-expressing aflatoxins. However, the fungi defences can be controlled by several methods. Articles showed efficiency of various of them. Good cultural practices and awareness raising are part of the preventive control. Synthetic chemicals such as insecticides and fungicides are chronically used in chemical control of fungal growth and prevent aflatoxins from being produced. Biological control is based on allelic recombination between toxic and atoxic strains. The use of microbial competition is focused on the natural predators of aflatoxic molds, most often lactic acid bacteria, and the natural control relies on the use of natural plant substances. Natural substances like aqueous or organic plant extracts that contain proteins, polyphenols, tannins, antioxidants, flavonoids, terpenes, and chelating ion as well as caffeic acid, gallic acid and ascorbic acid can be used to control fungal contamination currently. These molecules interfere with free radicals to slow down or even inhibit the production of reactive oxygen species significantly reducing the production of aflatoxins making them inactive. Implementation of the means of controlling fungal growth and producing aflatoxins will help make agriculture globally competitive and ensure food security. Key words: Natural substances, Aflatoxin B1, food poisoning, alternative control, biocontrol

Mosquitoes do not Like Bitter.

Chemical repellents play a crucial role in personal protection, serving as essential elements in reducing the transmission of vector-borne diseases. A biorational perspective that extends beyond the olfactory system as the classical target may be a promising direction to move. The taste system provides reliable information regarding food quality, helping animals to discriminate between nutritious and potentially harmful food sources, often associated with a bitter taste. Understanding how bitter compounds affect feeding in blood-sucking insects could unveil novel molecules with the potential to reduce biting and feeding. Here, we investigated the impact of two naturally occurring bitter compounds, caffeine and quinine, on the feeding decisions in female Aedes aegypti mosquitoes at two distinctive phases: (1) when the mosquito explores the biting substrate using external taste sensors and (2) when the mosquito takes a sip of food and tastes it using internal taste receptors. We assessed the aversiveness of bitter compounds through both an artificial feeding condition (artificial feeder test) and a real host (arm-in-cage test). Our findings revealed different sensitivities in the external and internal sensory pathways responsible for detecting bitter taste in Ae. aegypti. Internal detectors exhibited responsiveness to lower doses compared to the external sensors. Quinine exerted a more pronounced negative impact on biting and feeding activity than caffeine. The implications of our findings are discussed in the context of mosquito food recognition and the potential practical implications for personal protection.

Ants evade harmful food by active abandonment

Invasive ants, such as the Argentine ant, pose a severe economic and ecological threat. Despite advancements in baiting techniques, effectively managing established ant populations remains a daunting challenge, often ending in failure. Ant colonies employ behavioural immunity against pathogens, raising the question of whether ants can collectively respond to toxic baits. This study investigates whether ant colonies actively abandon palatable but harmful food sources. We provided two sucrose feeders, each generating a new foraging trail, with one transitioning to offering toxic food. Six hours later, ant activity on that path decreases, while activity on the non-toxic food and the trunk trail remains unaffected, excluding factors like population decline or satiation as reasons for the activity decline. Laboratory experiments confirmed that ants remained alive six hours after ingesting toxic food. Ant presence remains low on the toxic food path for days, gradually decreasing along the nearest section of the trunk trail. This abandonment behaviour minimises the entry of harmful food into the nest, acting as a protective social mechanism. The evasion of toxic bait-treated areas likely contributes considerably to control failures. Understanding the behavioural response to toxic baits is essential for developing effective strategies to combat invasive ant species.

Edukasi Zat Pewarna Pangan Berbahaya Di SMA Negeri 1 Sukadana

Introduction: In general, food coloring can be obtained from natural dyes and safe synthetic food dyes. Natural dyes are sometimes considered less stable and less durable than synthetic food dyes. Although there are synthetic dyes that are safe for food, there are still people who use materials that are not suitable for use in food. The use of harmful food dyes must be monitored and recognized for their existence. Harmful food dyes that are commonly found are textile dyes such as Rhodamine B or methanyl yellow. Because it is not a proper food coloring, consumption of foods containing this ingredient can cause a series of health problems for those who consume it, especially in a relatively long period of time. This education aims to allow students to distinguish foods that use natural coloring agents and artificial coloring agents. The results of this activity students can find out the characteristics of foods that use harmful dyes and know the prevention that can be done.
 Objective: The purpose of this service is for SMAN 1 SUKADANA students to know the dangers of using hazardous coloring agents in food.
 Method: The method used is providing education by providing material to students, tests before and after material on the Use of Harmful Coloring Substances in Food.
 Result: Increased knowledge and attitudes of students are tested through pre-test and post-test. There has been an increase in knowledge in students about the dangers of using harmful dyes in food in general by 20%, so that the realization of the purpose of this service is expected to be able to distinguish food ingredients that contain harmful coloring agents in food and those that do not contain harmful coloring agents in food. Basically, knowledge and attitudes also depend on the high or low level of education of students. Therefore, the existence of this counseling can help students in adding insight into the dangers of using harmful dyes. The implementation of this activity can run well due to positive support in the implementation process, where support from various parties and there are no obstacles whatsoever.
 Conclusion: The socialization activity on the use of dangerous coloring substances in food to students at SMAN 1 Sukadana received a good response from the school and also students. With outreach activities regarding this, students will understand and be more careful in choosing food to avoid these chemicals.

Acrylamide increases and furanoic compounds decrease in plant-based meat alternatives during pan-frying

Plant-based meat alternatives are gaining popularity as protein sources. However, pan-frying may lead to the formation of potentially harmful food contaminants. We investigated the formation of acrylamide and furanoic compounds in four different plant-based meat alternatives and two meat burger patties during pan-frying at 160 and 200 °C. The highest acrylamide contents (72. ± 7.7 and 69.2 ± 9.5 µg/kg, respectively) were found in soy flour- and sunflower-protein based patties fried at 200 °C. Unprepared pea and soy protein-based burger patties contained the highest furfural amounts (2832.8 ± 576.2 and 2683.0 ± 868.5 µg/kg, respectively). Furfuryl alcohol content was highest in soy flour-based patties and increased temperature-dependently up to 1120.9 ± 383.4 µg/kg. Based on the tolerable intake calculated by the EFSA Scientific Panel on Contaminants in the Food Chain, these amounts do not pose a health risk. Nevertheless, since plant-based novel food are being increasingly consumed, further investigations into the formation of food contaminants in novel processed foods are warranted.

Exploring Various Photochemical Processes in Optical Sensing of Pesticides by Luminescent Nanomaterials: A Concise Discussion on Challenges and Recent Advancements.

Food safety is a burning global issue in this present era. The prevalence of harmful food additives and contaminants in everyday food is a significant cause for concern as they can adversely affect human health. More particularly, among the different food contaminants, the use of excessive pesticides in agricultural products is severely hazardous. So, the optical detection of residual pesticides is an effective strategy to counter the hazardous effect and ensure food safety. In this perspective, nanomaterials have played a leading role in defending the open threat against food safety instigated by the reckless use of pesticides. Now, nanomaterial-based optical detection of pesticides has reached full pace and needs an inclusive discussion. This Review covers the advancement of photoprocess-based optical detection of pesticides categorically using nanomaterials. Here, we have thoroughly dissected the photoprocesses (aggregation and aggregation-induced emission (AIE), charge transfer and intramolecular charge transfer (ICT), electron transfer and photoinduced electron transfer (PET), fluorescence resonance energy transfer (FRET), hydrogen bonding, and inner filter effect) and categorically demarcated their significant role in the optical detection of pesticides by luminescent nanomaterials over the last few years.

Provenance blockchain for ensuring IT security in cloud manufacturing

Provenance blockchain is an evolving concept for protection of production, logistics, and supply chain networks from rogue Industrial Internet of Things (IIoT) devices. Such rogue IIoT devices are a recognized threat in the cloud manufacturing networks. In extreme cases, they can be used to cause industrial accidents. A brief version of provenance is about end-to-end tracking and tracing of data and the nodes involved in creating, modifying, transmitting, storing, and deleting it at specific times and locations. It provides an end-to-end verifiable and controlled computation for ensuring trustworthiness, quality, reliability, and validity of data. Provenance has existed in computing using logging software systems. This research is focused on threats to food supply chains between two countries. A scenario for protecting food supply chain from India to UAE has been modeled. This research recognized the threat of harmful food items getting mixed with flow of genuine products in a supply chain. The IIoT devices used to control the flow can be authenticated using the evolving provenance blockchain technology. With the help of recent design recommendations in the literature, a model design has been created and simulated in this research. Observations from the simulation revealed that TCP congestions and unpredictable turnaround time for assigning cryptographic keys to IIoT device sessions may have to be explored in future. A collaborative design between the two nations has been proposed. All IIoT devices not supporting cryptography will be eliminated from the cloud manufacturing and supply chain networks. Currently, this design may be used for one time registration only. Future studies may provide improved versions in which, repeated authentication and keys replacements may be implemented.

Bitter taste receptors of the zebra finch (Taeniopygia guttata).

Despite the important role of bitter taste for the rejection of potentially harmful food sources, birds have long been suspected to exhibit inferior bitter tasting abilities. Although more recent reports on the bitter recognition spectra of several bird species have cast doubt about the validity of this assumption, the bitter taste of avian species is still an understudied field. Previously, we reported the bitter activation profiles of three zebra finch receptors Tas2r5, -r6, and -r7, which represent orthologs of a single chicken bitter taste receptor, Tas2r1. In order to get a better understanding of the bitter tasting capabilities of zebra finches, we selected another Tas2r gene of this species that is similar to another chicken Tas2r. Using functional calcium mobilization experiments, we screened zebra finch Tas2r1 with 72 bitter compounds and observed responses for 7 substances. Interestingly, all but one of the newly identified bitter agonists were different from those previously identified for Tas2r5, -r6, and -r7 suggesting that the newly investigated receptor fills important gaps in the zebra finch bitter recognition profile. The most potent bitter agonist found in our study is cucurbitacin I, a highly toxic natural bitter substance. We conclude that zebra finch exhibits an exquisitely developed bitter taste with pronounced cucurbitacin I sensitivity suggesting a prominent ecological role of this compound for zebra finch.

Dietary preferences and analysis of the "plant-based food basket" among Ukrainian preteens and teenagers

The aim. The aim of the study was to analyze aspects related to a healthy diet and lifestyle, more specifically to the consumption of plant-based food among Ukrainian preteens and teenagers aged 10 to 17 years.
 Materials and methods. 231 individuals aged 10 to 17 participated in the study, 85 of them were boys, 146 were girls, all of them were residents of Kharkiv city at the time of participation in the study. The respondents were presented with a questionnaire regarding their attitude to a healthy lifestyle, in particular, rational nutrition. The χ2 test was used to analyze the qualitative data.
 Results and discussion. In the work, it was found that more than half of the respondents led only a partially healthy lifestyle, 60.3 % and 54.1 % among girls and boys, respectively. Only 11.6 % and 18.8 % of girls and boys followed the diet, although the majority of preteens and teenagers did not skip breakfast (80.8 % of girls and 89.4 % of boys). Unfortunately, the vast majority of subjects (58.9 % of girls and 56.5 % of boys) sometimes indulged in "harmful food". Also, the majority of preteens and teenagers consumed food between principal meals (83.6 % of girls and 72.9 % of boys), which, taking into account current ideas, should be considered an alarming trend. The main motivation for choosing food among preteens and teenagers was their own food tastes and family traditions, while food advertising was taken into account by just over 1 % of preteens and teenagers. Self-assessment of the health state shows that only 2/3 to ¾ of preteens and teenagers had no complaints about their health. No sex differences were observed for all the indicated characteristics. The most popular vegetable product among Ukrainian preteens and teenagers was potato, it was consumed by more than ¾ of preteens and teenagers, its popularity grew with age, which can be considered a negative trend (fast food). The least popular plant-based product among preteens and teenagers was seeds, consumed by only 19.9 % of girls and 8.2 % of boys. It was the only plant-based product, for which a statistically significant difference in consumption was found (2.4 times more common among girls than among boys). With age, the frequency of coffee consumption as an "adult drink" increased among preteens and teenagers, while a "leap" in the prevalence of its consumption can be seen when passing from 14-15 years to 16-17 years. Therefore, most of the results obtained should be considered as concerning ones.
 Conclusions. The consumption of plant-based food among Ukrainian preteens and teenagers is almost not associated with sex, while the consumption of some types of plant-based food is more closely related to the age of preteens and teenagers, which may indicate the continuation of the process of forming food habits in this ontogenesis period. The practical value of the work lies in potential corrective recommendations from doctors, valeologists, nutritionists, psychologists and other related specialists

Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component.

There has been an increase in the use of in vivo and in vitro intestinal models to study the pathophysiology of inflammatory intestinal diseases, for the pharmacological screening of potentially beneficial substances, and for toxicity studies on potentially harmful food components. Of relevance, there is a current demand for the development of cell-based in vitro models to substitute animal models. Here, a protocol for a basic, "healthy tissue" three-dimensional (3D) intestinal equivalent model using cell lines is presented with the dual benefit of providing both experimental simplicity (standardized and easily repeatable system) and physiological complexity (Caco-2 enterocytes with a supporting immune component of U937 monocytes and L929 fibroblasts). The protocol also includes paraffin embedding for light microscopic evaluation of fixed intestinal equivalents, thereby providing the advantage of analyzing multiple visual parameters from a single experiment. Hematoxylin and eosin (H&E) stained sections showing the Caco-2 columnar cells forming a tight and regular monolayer in control treatments are used to verify the efficacy of the model as an experimental system. Using gluten as a pro-inflammatory food component, parameters analyzed from sections include reduced monolayer thickness, as well as disruption and detachment from the underlying matrix (H&E), decreased tight junction protein expression as shown from occludin staining (quantifiable statistically), and immune-activation of migrating U937 cells as evidenced from the cluster of differentiation 14 (CD14) staining and CD11b-related differentiation into macrophages. As shown by using lipopolysaccharide to simulate intestinal inflammation, additional parameters that can be measured are increased mucus staining and cytokine expression (such as midkine) that can be extracted from the medium prior to fixation. The basic three-dimensional (3D) intestinal mucosa model and fixed sections can be recommended for inflammatory status and barrier integrity studies with the possibility of analyzing multiple visual quantifiable parameters.

Investigation of some basil genotypes in terms of their effect on bacterial communication system, and antimicrobial activity

The exponential growth of multiresistant bacterial strains creates the need to explore new or combined strategies to combat bacterial resistance. Medicinal plant-derived compounds against pathogenic bacteria may provide new, simple approaches to developing more environmentally friendly antimicrobial agents. Many researchers focus on exploring novel or combined strategies for combating bacterial resistance. Aromatic plants containing essential oils, such as basil, are often used as therapeutic agents in the pharmaceutical industry. Recent research has shown that basil is effective against certain harmful food phytopathogenic bacteria and has antimicrobial and anti-quorum sensing properties, which were investigated in this study. Our results have shown that the essential oil and ethanol extract of basil exhibits both antibacterial activity and anti-quorum sensing activity against some Gram-negative and Gram-positive bacterial species. It has also been found to have antifungal effects on C. albicans. Among the tested microorganisms, the genotypes of PI 531396, PI 296390, PI 414199, PI 253157, PI 296391, PI 652071, midnight, and Dino cultivars have been found to be more effective than other genotypes. The highest effect on quorum sensing system was found in Moonlight and Dino cultivars, PI 296391, PI 414199, PI 652070, PI 172997 and PI 190100 genotypes. Dendrogram analysis has shown that there is a relationship between different genotypes depending on microorganisms and anti-quorum sensing activity. Ames 29184, PI 207498, and PI 379412 genotypes were in the same group. Biplot analyses were performed to determine the relationship between the studied properties, and the results showed that more than 47% of the total variation was in all forms.

Bacteria and poisonous plants/fungi were the primary causative hazards of foodborne disease outbreaks: A five-year survey from Guangzhou, Guangdong

The burden of foodborne diseases is of serious concern. More effective and localized intervention policies for outbreak prevention and management are required; however, policy modification is hampered due to a lack of information on the epidemiological characteristics of outbreaks in Guangzhou. We collected data from 182 foodborne disease outbreaks reported in Guangzhou, China from 2017 to 2021 to investigate the epidemiological characteristics and associated factors. Nine outbreaks were serious enough to be labelled as level IV public health emergencies, all of which were associated with canteens. In terms of the number of outbreaks, morbidity and clinical medical needs, bacteria and poisonous plants/fungi were the primary causative hazards of outbreaks, and were found mostly in foodservice establishments (96 %, 95/99) and private homes (86 %, 37/43) respectively. Surprisingly, Vibrio parahaemolyticus was primarily identified in meat and poultry products rather than in aquatic products in these outbreaks. Patient specimens and food samples were among the most common sources of detected pathogens in foodservice establishments and private homes. Cross-contamination (35 %), improper processing (32 %) and equipment/utensil contamination (30 %) were the top three risk factors for outbreaks related to foodservice establishments, while accidental ingestion of poisonous food (78 %) was the most common risk factor in private homes. Based on the above epidemiological characteristics of the outbreaks, key foodborne disease intervention policy points should be to raise public awareness of harmful food and avoid risk behaviour, improve handler hygiene training, and strengthen the hygiene management and supervision of kitchens, especially canteens in collective units.

Perspective of Undergraduate Students of University in Edo State on the Side Effect of Food Additive on Human Health

This study examined the perspectives of undergraduate students on the side effects of food Additives to human health. Two research questions were raised to guide the study. The population of this study comprised of all undergraduates students in Edo State. The study adopted the descriptive design. The sample size for this study comprised one hundred undergraduate students in Ambrose Alli University Ekpoma, Edo state, Nigeria. using random sampling techniques. Questionnaire was used to collect data for this study, the questionnaire was made up of two parts, section A and B, section A contains demographic information of the respondents, section B contains research area of investigation, the instrument was validated by experts in Home Economics Education, department of vocational and technical education, AAU. Split half method was used to obtain reliability coefficient of 0.76 . The researcher personally administered the copies of the questionnaires to the respondents. It contains twenty items. The questionnaires were collected immediately to avoid loss of data’s. The findings of this study revealed that most undergraduate students are not aware of side effect of food additives to human health, it was also revealed that the respondents consumed some harmful additives in large quantity, which is dangerous to human health, it was recommended among others that Government should create awareness on the side effect of food additive to human health. The public should avoid harmful food additives and reduce consumption of processed food. Keywords : Food Additives, Types of Food Additives, Effects of Food Additives, Undergraduate Students. DOI: 10.7176/JEP/14-15-06 Publication date: May 31 st 2023

KONSUMSI AIR PUTIH DAN MINUMAN MANIS, SOFT DRINK/MINUMAN BERKARBONASI, MINUMAN BERENERGI, MIE INSTAN DAN MAKANAN OLAHAN PADA REMAJA

The increasing prevalence of non-communicable diseases from year to year is related to the consumption of food and drinks. Drinks are anything that can be consumed and can quench thirst, usually in liquid form. Instant noodles are types of food that contain high fat and sodium, while processed foods (meat, chicken, fish) are foods that contain a lot of harmful food additives. The purpose of this study was to describe the consumption of water, sweet drinks, soft drinks/carbonated drinks, energy drinks, instant noodles and processed foods (meat, chicken, fish). This type of research is descriptive research, the data is collected using a semi FFQ (Food Frequency Questionnaire) questionnaire. Data analysis was univariate, namely the percentage and average weight of adolescent food and beverage consumption, then categorized. The results of this study are more water consumption in the less category, sweet drinks, soft drinks/carbonated drinks, energy drinks, instant noodles and processed foods (meat, chicken, fish) are in the rare category.

Knowledge of Subang salted fish sellers and prevention strategies for formalin abuse in salted fish

Fish is a perishable commodity. Producers can process fish into salted fish to make it more durable, but producers still add harmful food additives to increase the product’s shelf life. The government prohibits the use of formalin as a food additive. This study aimed to identify salted fish traders’ knowledge of formalin abuse and obtain a strategy to minimize formalin abuse in salted fish. The research method was carried out by giving questionnaires, interviews and using the Strategic Assumption Surfacing and Testing (SAST) method. The results showed that the seller’s knowledge was still low regarding the salted fish products sold, food additives, food product preservation, the lack of socialization about food safety, and the characteristics of formalin products. The chosen strategy is based on the level of importance and the level of certainty to minimize the misuse of formalin, namely socialization to sellers and buyers regarding formalin, the dangers and how to use it and the manufacture of portable detection kits/tools that are easy to use and accurate results.