Titanium alloys are widely used in aerospace, chemical, biomedical and other important fields due to outstanding properties. The mechanical behavior of Ti alloys depends on microstructural characteristics and type of alloying elements. The purpose of this study was to investigate the effects of different Cu contents (2.5 wt.%, 7 wt.% and 14 wt.%) on mechanical and frictional properties of titanium alloys. The properties of titanium alloy were characterized by tensile test, electron microscope, X-ray diffraction, differential scanning calorimetry, reciprocating friction and wear test. The results show that the intermediate phase that forms the eutectoid structure with α-Ti was identified as FCC Ti2Cu, and no primary β phase was formed. With the increase of Cu content, the Ti2Cu phase precipitation in the alloy increases. Ti2Cu particles with needle structure increase the dislocation pinning effect on grain boundary and improve the strength and hardness of titanium alloy. Thus, Ti-14Cu shows the lowest elongation, the best friction and wear resistance, which is caused by the existence of Ti2Cu phases. It has been proved that the mechanical and frictional properties of Ti-Cu alloys can be adjusted by changing the Cu content, so as to better meet its application in the medical field.