Geological and structural influences on groundwater flow and quality were evaluated in the present study in the hardrock regions of Tirunelveli District, southern India. Groundwater is a major source of freshwater in this region to cater to the requirements of domestic and agricultural activity, as there are no surface water resources. Geologically, the area is characterized by charnockites and garnetiferous biotite gneiss. Groundwater in this region is found to occur in the weathered portion under unconfined condition and in fractured/fissured portions under unconfined to semi-confined condition. Existence of deep-seated fractures are minimal. Lineaments/dykes play a major role in the occurrence and movement of groundwater in the region. Lineaments/dykes of the study area can be broadly divided into two types: north–south and west–east oriented structures. Analysis and field observations revealed that the north–south dykes act as a barrier of groundwater while the west–east oriented structures behave as a carrier of groundwater. Both quality and quantity of groundwater is different on the upstream and downstream sides of the dyke. Hence, it is conclusive that the west–east oriented dykes in this region are highly potential and act as a conduit for groundwater movement from recharge areas to the discharge area.