Background: Bisphenol-A (BPA) is the building block of polycarbonate plastics, a hard plastic used to make numerous consumer products, including most baby bottles and water bottles. Objective: Assessing the adverse effect of oral administration of BPA on liver and kidney of adult male albino rats and evaluation of the role of vitamin C and thymoquinone (TQ) in alleviating the possible detrimental effects of BPA. Material and Methods: Fifty six adult male albino rats of local strain were housed in 14 suitable metal cages(20 ×32× 20 cm for every 4 rats).They were divided into seven equal groups : The 1st group was served as a control group, the 2nd group was treated with bisphenol-A, the 3 rd group was normal and treated with vitamin C alone ,the 4 th group was normal and treated with thymoquinone alone, the 5 th group was administered by bisphenol-A and treated with vitamin C, the 6 th group was administered by bisphenol-A and treated with thymoquinone and the 7 th group was administered by bisphenol-A and treated with both vitamin C and thymoquinone. All animals were treated for four weeks. Results: Administration of bisphenol-A (50mg/Kg body weight orally) to rats resulted in an increase in alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea , creatine, and hepatic and renal MDA level, and decreased reduced glutathione (GSH) and superoxide dismutase (SOD) contents of the liver and kidney as compared to control group. In contrast, the administration of vitamin C (500 mg/ kg body weight) and/ or thymoquinone(10 mg /kg body weight) to bisphenol-A treated rats attenuated the toxicity of bisphenol. This alleviation was more pronounced in the bisphenol-A group treated with both vitamin C and thymoquinone. Conclusion: BPA has detrimental effect on liver and kidney of adult male albino rats. It supported the possibility that the synergistic effect of thymoquinone and vitamin C played a notable role in protecting the liver and kidney against BPA-induced toxicity and oxidative stress in male rats.