Hand Hygiene Practices Research Articles

Perspectives on hand hygiene in Belizean healthcare facilities during the COVID-19 pandemic: a qualitative evaluation with healthcare workers

ABSTRACT The World Health Organization recommends healthcare workers (HCWs) practice hand hygiene (HH) while providing care. Making alcohol-based hand rub (ABHR) available at points of care is recommended during times of high patient volume, such as the COVID-19 pandemic. In low- and middle-income countries, such as Belize, there may be limited access to HH materials within healthcare facilities. This paper examines the motivators and barriers to HH among HCWs in the 11 largest public healthcare facilities in Belize and HCWs' experiences with an intervention. In 2021, focus group discussions (FGDs) gathered HCWs' HH perceptions and preferences. An intervention was then implemented to increase ABHR access and HH training for HCWs. Post-intervention endpoint FGDs in 2022 documented HCWs' experiences with interventions. Baseline FGDs revealed that self-protection and protection of one's household members from illness were key motivators for HCWs' HH practice. Insufficient time, inadequate access to HH supplies, and gaps in education were barriers to practicing HH. At endpoint, participants appreciated increased access to ABHR and its convenience but did not like ABHR's effect on hands. Experiences with the training were mixed. To improve HCWs' HH practices, HH interventions should be tailored to HCWs' context and learning preferences.

Prevalence of SARS-CoV-2 and Associated Factors Among Individuals During the Second Wave of Infection in Yaounde, Cameroon

Background: The first case of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported in Cameroon on March 5, 2020, in Yaounde. Since then, viral propagation occurred nationwide with a higher burden in urban settings and limited evidence on transmission patterns. Our objective was to determine the prevalence of SARS-CoV-2 and associated factors among individuals in urban settings of Cameroon during the second wave of the pandemic. Methods: a population-based cross-sectional survey was conducted from January 18 to April 26, 2021, among 807 consenting individuals living in four major districts of the city of Yaoundé. Out of 711 nasopharyngeal swabs collected, 671 tests were performed by SARS-CoV-2 ribonucleic acid (RNA) extraction and real time-polymerase chain reaction (RT-PCR) at the National Public Health Laboratory (NPHL). Data were analysed using SPSS v21.0, with p < 0.05 considered statistically significant. Results: overall SARS-CoV-2 RNA positivity rate was 11.62% (78/671), without any significant difference between males and females with 10.9% versus 12.20% respectively (OR = 0.87, p = 0.5). However, clinical status was associated with SARS-CoV-2 RNA positivity rate in symptomatic (26.3%) vs. asymptomatic (9.7%) individuals (Odds Ratio [OR] = 3.30, p = 0.0001). Other factors associated with SARS-CoV-2 RNA positivity include exposure to more than 15min with confirmed cases, wearing a non-conventional facemask (p ˂ 0.05), the practice of occasional hand hygiene before and after contact with patients (OR = 3.60, p < 0.0001) or always as recommended (OR = 0.28, p = 0.0001), hand hygiene before and after occasional contact with objects (OR = 2.05, p = 0.0043) or always as recommended (OR = 0.48, p = 0.0027), surgical mask (OR = 0.17, p = 0.013) and standard mask (p = 0.026). Conclusion: From this urban setting of Cameroon, coronavirus disease 2019 (COVID-19) reached an alert burden (10-20%) during the second wave of the pandemic at the district level. Interestingly, SARS-CoV-2 infection was driven by the presence of symptoms, close contact with confirmed cases, limited adherence to recommended barrier measures, and the use of non-conventional facemasks.

Evaluation of Hand Hygiene Beliefs and Practices Among Healthcare Workers in a Training and Research Hospital

Evaluation of Hand Hygiene Beliefs and Practices Among Healthcare Workers in a Training and Research Hospital

An agent-based modeling approach for infection prevention predesign: simulating the spread of pathogens between humans and the environment in an Intensive Care Unit

ABSTRACT This study aims to investigate infection prevention measures in healthcare environments, focusing on the role of surface materials and hand hygiene compliance. To address this goal, an Agent-Based Model was developed to examine the combined effects of human factors and environmental interventions on the transmission of three types of healthcare-associated bacteria in an Intensive Care Unit (ICU). The model evaluates five key prevention measures: surface material selection, daily and terminal cleaning, hand hygiene compliance, and the use of personal protective equipment (PPE). Hand hygiene was chosen as the primary variable for model validation due to the extensive computational and field research available in this area. It also serves as a bridge between material design and human factors in infection transmission risk. Simulation data were compiled from each run by gathering the average value for the number of infected patients and contaminated environment over different scenarios. After validating the model, patients infectivity and environmental contamination associated with commonly used surface materials; namely quartz, copper-impregnated surfaces, plastic laminate, and stainless steel; were assessed under two scenarios of baseline and enhanced hand hygiene compliance. The findings underscore the importance of surface material choices in healthcare settings, suggesting that the most effective infection control strategies combine improved hand hygiene practices with the strategic use of materials in areas prone to bacterial contamination. These insights advise healthcare designers to adopt strategies that integrate both behavioral interventions and environmental components to minimize the risk of contamination and pathogen spread within healthcare environments.

Study on hand disinfection in inpatient geriatric care on the superiority of cold plasma aerosol versus alcohol-based disinfection methods in a parallel group design

The introduction of fundamental hygiene protocols within the healthcare sector during the nineteenth century led to a significant reduction in mortality rates. Contemporary advancements, such as alcohol-based sanitizers, have further enhanced hand hygiene practices. However, these measures are often overlooked in nursing facilities, resulting in low staff compliance rates and increased cross-infection rates. Novel approaches, such as cold plasma hand disinfection, present promising alternatives due to their minimal skin damage and economic benefits. This study aims to compare the disinfectant efficacy of cold plasma aerosol under practical application conditions with an alcoholic hand disinfectant listed by the Association for Applied Hygiene. The microbial count on participants’ hands was measured, with particular attention paid to the spontaneous occurrence of fecal indicators and the presence of potentially infectious bacteria. A t-test for independent samples was conducted to determine whether there was a significant difference between the two cohorts regarding the research question. Statistical analysis revealed that the mean log colony-forming unit (CFU) values were significantly lower in the test cohort using only the cold plasma method for hand disinfection compared to the cohort using conventional alcohol-based hand disinfection. Moreover, it was demonstrated that, unlike alcohol-based hand disinfection, cold plasma application ensures the effective elimination of Staphylococcus aureus. The findings indicate that staff utilizing plasma disinfection have an average bacterial count that is 0.65 log units lower than those who regularly use alcohol-based hand disinfection. In addition to the efficacy of cold plasma disinfection, its superiority over alcohol-based hand disinfection was also established. Beyond offering economic and logistical advantages, cold plasma disinfection provides additional health benefits as it does not induce skin damage, unlike alcohol-based hand disinfection.

Clinical Effectiveness of a 3-Step Versus a 6-Step Hand Hygiene Technique: A Randomized Controlled Cross-over Study

Abstract Background The aim of this study is to assess the clinical effectiveness of the 3-step hand hygiene (HH) technique (3-HT) compared with the 6-step HH technique (6-HT; World Health Organization 6-step technique) using an alcohol-based hand rub. Methods A randomized controlled crossover trial was conducted from November to December 2023 in 10 wards of a tertiary A-level hospital according to CONSORT guidelines. The 240 healthcare workers (HCWs) were randomly divided into the 3-HT intervention group or the 6-HT control group. The trial was conducted in 2 stages, and the effectiveness of each indicator in the 2 groups was compared after a washout period of 2 weeks. Results Compared with the 6-HT, the 3-HT has demonstrated significant superiority in all indicators of HH compliance as well as the accuracy rate of HH practices. The total HH median times for the 3-HT and 6-HT were 16.00 (interquartile range, 15.00–20.00) and 32.50 (30.00–40.00) seconds, respectively (P < .05). The reduction factors for bacterial colony-forming unit counts did not differ and the colony-forming unit counts were not significantly different. Bacillus, Staphylococcus, and Micrococcus were detected before and after the use of hand rubs. HCWs preferred the 3-HT over the 6-HT. There were no significant difference in healthcare-associated infections rate between the 2 techniques. Conclusions The 3-HT was significantly superior to the 6-HT in terms of the HH eligibility rate, compliance, and HH time. The safety and feasibility of the 3-HT were verified by assessing microorganism count.

Assessment of Knowledge, Perception, and Compliance with Hand Hygiene Practice among Health Care Workers at National Liver Institute, Menoufia University

Assessment of Knowledge, Perception, and Compliance with Hand Hygiene Practice among Health Care Workers at National Liver Institute, Menoufia University

Food safety profile of university food handlers in Bangladesh: A multicenter cross-sectional study

The current study aimed to explore the food safety knowledge, attitudes, and practices of university food handlers in Bangladesh. To achieve this goal, a cross-sectional study was formulated to be carried out among food handlers at different public universities. Data collection was conducted through face-to-face interviews utilizing a structured questionnaire. A total of 788 food handlers participated in the study and were considered for performing all the statistical analyses (descriptive statistics, MannWhitney U test, KruskalWallis H test, multiple logistic regression, and Spearman rankorder correlation). According to the study findings, almost 20.7 % of food handlers lacked formal education, and only 22.8 % had participated in food safety training. Approximately 31.5 % and 28.4 % of the food handlers had good knowledge and good practices, respectively. The majority of the food handlers had poor knowledge about foodborne diseases and foodborne pathogens. Most food handlers had poor hand hygiene practices. Almost all food handlers did not wear caps, gloves, aprons, or masks while handling food. However, despite of having poor knowledge and practices, food handlers (69.8 %) had comparatively good attitudes toward food safety practices, which indicates that adequate support and motivation from food safety authorities can significantly improve preventive practices among them. Therefore, it is recommended that food safety authorities accelerate their activities and arrange hand-based training and educational programs for food handlers to enhance food safety knowledge and practices.

The Effect of Simulation-Based Training on the Hand Hygiene Knowledge and Practices of Palliative Caregivers:A Double-Blind, Randomized, Controlled Single-Center Study.

This study aimed to investigate the effect of simulation-based training on hand hygiene knowledge and practices among palliative caregivers. The study was conducted with 60 caregivers in a palliative care clinic between December 2022 and September 2023. The participants were divided into two groups by simple randomization. The intervention and control groups received the same hand hygiene theoretical education and demonstration. The intervention group also received additional simulation-based hand hygiene practices recommended by the World Health Organization. A pretest-posttest design was used to assess hand hygiene knowledge and practices. Data were collected with personal information, hand hygiene knowledge, and hand hygiene practice forms. Analysis of covariance was performed to compare posttest scores between the groups. Simulation-based hand hygiene training programs offer an effective and feasible strategy to improve the hand hygiene knowledge and practices of caregivers. It should be integrated into clinical areas to increase palliative caregivers' hand hygiene knowledge and practices. Evidence-based practices can be improved by increasing randomized controlled studies on the effectiveness of simulation-based hand hygiene training for caregivers. Trial Registration: The study was registered at ClinicalTrials.gov with registration number NCT05848596.

Impact of hand hygiene interventions on handwashing practices and microbial risk: A study in an orphanage-based school in Pakistan

Orphaned children are often deprived of quality care, making them more susceptible to diseases due to inadequate hand hygiene. The study aimed to assess the prevalence of hand hygiene practices and detect bacterial loads on children's hands before and after hygiene interventions in an orphanage school. The study enrolled all the orphan children registered with the Save Our Souls children's orphanage school in Pakistan. Handwashing practices and swab samples from the hand was collected to evaluate the impact of handhygiene practices on bacterial load. The Quantitative Microbial Risk Assessmentmodel was used to predict the health risk. The study identified the 2 most common bacteria: Staphylococcusaureus and Escherichiacoli. The bacterial contamination was significantly reduced after the intervention (Saureus 166 Colony-forming unit (CFU) /mL and Ecoli 185 CFU/mL). The higher bacterial ingestion rate was attributed to hand contamination and increased bacteria transfer from hand to mouth. The multicomponent hand hygiene intervention showed improvement in accessibility to hand hygiene resources and practices. The findings underscore the need for hygiene interventions in orphanage schools to improve health and educational outcomes.

Socioeconomic Risk Factors in Neurocysticercosis.

NCC is responsible for around 30% of active epilepsy in endemic countries such as India. Knowing socioeconomic factors associated with NCC can help in decreasing the incidence of this disease. We intend to study socioeconomic risk factors in NCC patients and how these factors are different from patients without NCC. Consecutive 100 adult patients of NCC presenting to neurology services with probable NCC (symptomatic or asymptomatic) along with age- and sex-matched controls were interviewed with preset proforma and then data was compiled and analyzed statistically. The NCC group had significantly more people with lesser education level (53%) as compared to control group (42%) (P = 0.002), lower socioeconomic status, poor hand hygiene practices, and use of contaminated vegetables (40% NCC group, 1% control group, P < 0.0001). Open defecation practice was also more among NCC cases. Lower education and socioeconomic status, poor hand hygiene, and eating undercooked contaminated vegetables are modifiable factors that are more prevalent in patients of NCC compared to controls. Social interventions aiming at these will help to reduce NCC patients in our country.

Health Risks Associated with Microphone Sharing: A Survey of Awareness, Perception and Preventative Actions among Christian Church Choir Members in Taraba State, North-East, Nigeria

The aim of this study was to investigate the awareness and health actions related to the risks of microphone sharing among Christian choir members in Taraba State, Nigeria. Grounded in the Health Belief Model, the study utilised a descriptive survey design, sampling 225 choir members from 10 randomly selected churches across Taraba State, Northeast Nigeria. Data were collected via online questionnaires and analysed using simple descriptive statistics. The findings reveal a low level of awareness about the health risks associated with sharing microphones. The respondents generally demonstrated a negative perception towards these risks, with many expressing disbelief in the potential for disease transmission through shared microphones. Additionally, there was a notable lack of adherence to precautionary measures. The study found that microphones were infrequently cleaned, microphone foams were rarely replaced, and hand hygiene practices—such as hand washing and the use of sanitiser—were not routinely observed. The use of face masks and shields among choir members was also reported as rare. These findings have significant implications for public health practices within religious communities. They underscore the urgent need for increased awareness and education about the potential health risks of microphone sharing. Thus, the study recommends implementing regular cleaning protocols, ensuring the replacement of microphone foams, and promoting personal hygiene measures, including hand sanitisation and the use of face masks or shields during group singing activities. These measures are essential for mitigating health risks and ensuring the well-being of choir members.

Trends in the hand hygiene practices using alcohol-based hand rubs in Japanese hospitals before and after the novel coronavirus pandemic: an observational study using national surveillance data

Healthcare-associated infections (HAIs) are a global concern in healthcare facilities, and hand hygiene (HH) using alcohol-based hand rubs (ABHR) is fundamentally crucial for their prevention. While previous studies report improvements in HH compliance amid the COVID-19 pandemic, the real situation in Japanese medical settings remains unclear. This observational study sought data from the Japanese national surveillance, focusing on ABHR use in hospitals before and after the COVID-19 pandemic. Data were retrieved from facilities certified to receive the Additional Healthcare Reimbursements for Infection Prevention and Control I. The study spanned five years (2019-2023), segmented quarterly, and employed Joinpoint regression analysis to assess the annual percentage change (APC). Overall, ABHR use per patient per day significantly increased both in critical care units and general wards amid the pandemic. However, the APC in the critical care units demonstrated a downward trend from Q4 of 2021 to Q1 of 2023, and ABHR use in general wards remained below the amount of WHO recommendations. This trend analysis highlighted recent patterns of ABHR use in Japanese hospitals by comparing pre- and post-COVID-19 periods. Although increases in ABHR use were observed over time, sustained efforts to promote HH compliance are necessary, particularly in general wards.

Determinants of hand hygiene compliance among healthcare workers in intensive care units: a qualitative study

BackgroundPracticing hand hygiene is a cost-effective method to decrease the occurrence of Healthcare-Associated Infections (HAIs). However, despite their simplicity, adhering to hand hygiene methods among healthcare workers (HCWs) can be highly challenging. We aim to examine the factors influencing hand hygiene compliance as perceived by HCWs working in the intensive care units (ICUs) at several major hospitals in Riyadh, Saudi Arabia.MethodThis qualitative study was conducted by adopting a content analysis to examine the interviews of HCWs who are currently working in the ICUs of various major hospitals located in the capital city of Riyadh, Saudi Arabia.ResultsWe interviewed 49 HCWs working in ICUs, with an average age of 38 and 8 years of experience. The HCWs comprised doctors (n = 12), anesthesiologists (n = 6), and nurses (n = 31). There were 34 females and 15 males among the participants. Our analysis revealed several factors that impact hand hygiene compliance, including individual, work/environment, team, task, patient, organizational, and management concerns. Several obstacles and possibilities for enhancement have been identified.ConclusionThe results of this study would enhance our comprehension of hand hygiene practices and serve as a foundation for creating future strategies and assessment methods to enhance compliance with hand hygiene protocols in ICUs.

A Review of the Difficulties Faced by Low and Middle-income Countries in Hygiene and Healthcare Practices.

Hygiene has been identified as an important step in anticipating health-related pol-lution, with conflicting reports about consistent sanitation standards in the nation. This narrative review of published studies has led to the discussion of hand hygiene and health practices, as well as the identification of necessary methods that are available in low and middle-income countries. An effective survey of accessible information is conducted in this way based on re-view questions. A number of 372 articles were found on the web, and 32 articles were used in the final analysis. Overall, the standard of hand hygiene is set at 20.49%. Difficulties identified for the people in low or middle-income countries are poor awareness and sound information about hand hygiene, lack of resources including water, soap, hand scrubbing gel, etc., as mis-interpretations related to hand hygiene practices. People are paying more attention to healthcare-related illnesses as it becomes increasingly clear that most of them may be pre-vented. The free evidence-based practice states that to lower the danger of contamination, hand hygiene should be properly adhered to. The difficulties recognized in this review are reliable, with the discoveries of studies that have been led somewhere else. By conducting a basic ex-amination of the "Clean Care is Safer Care" as an excellent arrangement of WHO's global initiative activity on patient well-being programs, developing nations will have more time to consider the essential approaches for the use of fundamental disease prevention exercises in our health care settings.

Evaluating the tolerability and acceptability of a locally produced alcohol-based handrub and hand hygiene behaviour among health workers in Sierra Leone: a longitudinal hospital-based intervention study

BackgroundAlcohol-based handrub (ABHR) is the gold standard for hand hygiene (HH) and is a cornerstone of infection prevention and control (IPC) strategies. However, several factors influence the efficient use of ABHR by health workers. This study evaluated the tolerability and acceptability of a locally produced ABHR product and HH behaviour among health workers.MethodsA longitudinal hospital-based intervention study was conducted in accordance with the WHO’s standardized protocol for evaluating ABHR tolerability and acceptability (Method 1). Sixty health workers across 4 hospitals in Sierra Leone were observed over a 30-day period at three separate visits (days 1, 3–5, and 30) by trained observers. The outcomes of interest included skin tolerability and product acceptabilityevaluated using subjective and objective measures.ResultsObjective and subjective evaluations demonstrated strong skin tolerability and high acceptability with the product. At all three visits, the skin tolerability score assessed by trained observers was < 2 in ≥ 97% of participants, exceeding the WHO benchmark score (BMS = < 2 in ≥ 75%). Participants’ self-evaluations of overall skin integrity were 97% (visit 2) and 98% (visit 3) for scores > 4 (BMS = > 4 in ≥ 75%). The primary acceptability criteria increased up to 95% (colour) and 88% (smell) at visit 3 (BMS = > 4 in ≥ 50%). Despite high acceptability, the product’s drying effect remained low at 52% and 58% during visits 2 and 3, respectively (BMS = > 4 in ≥ 75%). There were positive HH behaviours (n = 53, 88%), with more than half (n = 38, 63%) of them exhibiting HH at almost every HH moment. The mean ABHR was notably high (76.1 ml, SD ± 35), especially among nurses (mean = 80.1 ml) and doctors (mean = 74.0 ml).ConclusionThe WHO-formulated, locally produced ABHR was well tolerated and accepted by health workers. These findings support the continuous utilization of evidence-based, cost-effective hand hygiene interventions in resource-limited settings. High handrub consumption and frequent HH practices were noticeable HH behaviours. Further research is recommended to optimize product formulations for skin dryness and investigate the association between ABHR consumption and hand hygiene compliance.

Assessment of Knowledge, Attitude, and Practice of Hand Hygiene Among Medical Students in a Tertiary Care Hospital.

Healthcare-associated infections (HCAIs) and emerging multi-drug resistance in nosocomial pathogens are perceived as a serious public health threat. Hands of healthcare workers (HCWs) become routinely colonized during patient care, serving as vehicles for transmission and leading to HCAIs.Hand hygiene (HH) is a globally accepted tool to avoid the broadcast of dangerous microorganisms and prevent HCAIs. A cross-sectional study was carried out to evaluate the knowledge, attitude, and practice toward HH among the medical students at Saveetha Medical College, Chennai, India. A 25-item validated questionnaire survey was formulated and circulated to100 medical students of all four academic years. There were 100 responses to the survey, and 44 (44%) participants performed HH appropriately for 20 seconds with alcohol-based hand rub. In our study, 25％ of the participants revealed that HH practices were not followed during emergencies. Many participants (40%) stated that the lack of sink, soaps, alcohol-based sanitizers, paper towels, and water is the reason for not performing HH. The gross knowledge of HH of the participants is moderate, but there were gaps between the knowledge and practice. Hence, it is essential to conduct structured training sessions and surveillance programs for medical students to address these gaps in knowledge and the correct HH procedures.

Determination of the effects of hand hygiene education given to nursing students in intensive care unit on hand microbiota

Purpose: This study is conducted with the purpose of comparing fourth-year nursing students' hand hygiene practices and beliefs with their hand flora. Additionally, we aim to assess the effectiveness of hand hygiene education in this context. Material and Methods: This research has been conducted using a pre-test, post-test, and control group quasi-experimental design. The Hand Hygiene Application Inventory and the Hand Hygiene Belief Scale administered as pre-tests. Students underwent tape stripping and swab methods to collect samples for their hand flora before any interventions. A post-test was conducted for the experimental group. Results: The hand hygiene beliefs and practices of both groups were similar before training. When the Hand Hygiene Application Inventory and Hand Hygiene Belief Scale scores before and after the training were examined, an increase was observed in the scale scores after the training. In this study, the microorganisms present in the general hand flora were identified simplistically, and the changes were observed after hand hygiene education. Furthermore, the study shed light on the structure of hand flora after education in terms of microbial load. Conclusion: It has been experimentally determined that there is a significant decrease in hand microbiota load and pathogenic groups with hand hygiene education.

Effectiveness of sensing gloves–applied virtual reality education system on hand hygiene practice: A randomized controlled trial

We developed a virtual reality (VR) education system and evaluated its clinical utility for promoting hand hygiene practices. This prospective, 2-week, randomized controlled study conducted at Okayama University Hospital, Japan, from November 2023 to January 2024, involved 22 participants (18 medical students and 4 residents). A fully immersive 360° VR system (VIVE Pro Eye) using a head-mounted display and sensing gloves was used to develop 3 healthcare tasks in a virtual patient room-Environmental Cleaning, Gauze Exchange, and Urine Collection. After monitoring all participants' baseline usage data of portable hand-rubbing alcohol in the first week, we randomly assigned them into 1:1 groups (VR training and video lecture groups). The primary outcome was differences in hand-rubbed alcohol use before and after intervention. Before the intervention, alcohol use did not significantly differ between both groups. After the intervention, a significant increase in alcohol use was observed in the VR training group (median: 8.2g vs16.2g; P=.019) but not in the video lecture group. Our immersive 360° VR education system enhanced hand hygiene practices. Infection prevention and control practitioners and digital technology experts must collaborate to advance the development of superior educational devices and content.

KAP study on hand hygiene in the era post COVID-19 SARS among general population.

Background: Hand hygiene accounts one of the most effective ways of keeping diseases at bay. As of now, this practice stands as a key cornerstone post COVID-19 era, thus helping to break the chain of contact transmission of such deadly outbreak’s outcome. Aims and Objectives: To assess knowledge, attitude and practices (KAP) relating to hand hygiene among general population post COVID-19 era. Materials and Methods: A cross-sectional study was done among general population of our setup over a span of 2 months by distributing online a pre-designed, pre-validated and semi-structured Google Form survey questionnaire having questions based on knowledge (10), attitude (07) and practices (06) relating to hand hygiene post COVID-19 era. Individuals enrolled were tech-savvy with access to e-mail and internet facility, and able to understand English language. Results: Data from responses of 545 individuals among our general community was collected and analyzed, out of which majority (88.9%) responders already had a basic insight about hand hygiene. 91.2% responders clearly believed hand hygiene is important to prevent the infection spread from contact. While 25.7% responders answered 40-60 seconds as minimum time required for hand washing, comparable majority (48.9%) marked it as 20-40 seconds. 70.9% responders were strongly against use of gloves as a substitute for hand washing. Interestingly, many had a mixed say regarding time span for which an alcohol-based hand sanitizer be rubbed onto hands viz. 32.3% stating it as 10 seconds, 27.7% as 20 seconds, 21.2% as 5 seconds and 18.8% as 15 seconds, respectively. Majority (85.4%) voted hand washing as better and probably the best mode of maintaining hand hygiene. Majority (93.1%) responders felt the need of practicing hand hygiene possibly at all the times. While most of our study population practiced hand hygiene thoroughly, 77.5% responders said that it positively affected their life also. However, in contrast, only 57.4% responders washed their hands after using public transport/money exchange, while 39.3% practiced it only sometimes. Conclusion: Our study highlights that despite of a positive relation between the awareness among general masses with regard to positive attitude about hand hygiene post COVID era, there was a mixed response about practice of the same, thus stressing on the need to reinforce such practices with due clarity so that the message goes loud and clear among public at large for breaking the chain of COVID transmission till date. Further our study can aid decision-makers in forecasting the effectiveness of infection mitigation strategies or in defining valid changes based on the practices, attitudes, and understanding of the intended population.