Background Automated touchless dispensing and closed-refill systems minimize touch points that help prevent cross contamination events and reduce dispenser contamination with clinically significant pathogens in healthcare settings. Additionally, most consumers prefer touchless dispensing when using public restrooms. However, many healthcare settings still utilize manual dispensers for soap and sanitizer. Some even have open, bulk, refillable dispensing systems and manual pumps and dispensers. A finding of a clinical in-use study revealed a significant reduction in the incidence of healthcare associated infections (HAIs) following the conversion to automated touchless soap and sanitizer dispensing systems with premeasured dosage amounts in a long-term care (LTC) care facility. Methods A LTC facility was qualified as a testing site for clinical in-use study of hand hygiene (HH) products’ impact on healthcare workers’ (HCWs) hands. The facility replaced all HH manual dispensing systems and locally purchased hygiene products with automated touchless dispensers, closed-refill systems, and quality formulations. In addition to the biophysical measurements of HCWs hands using bioengineering instrumentation, the baseline incidence of HAIs was recorded prior to the intervention and surveillance continued from 2014 to 2018. HAI rates provided by the testing site were compared to the baseline data collected in 2014 with specific emphasis on the infection outbreak season from October to December in each year. Results Descriptive data analysis of HAI incidence rates revealed significant and sustained reduction in HAI rates from 6.7% in October through December of 2014 to 1.6 % the same period in 2015, 2.8 % in 2016 and 2.4 % in 2017. Conclusions Converting the HH framework from manual dispensing to automated touchless dispensing with closed refill systems during a clinical-in-use study resulted in a sustained reduction of HAI rates over a 3-year period. Increased awareness of HH, touchless hygienic dispensing systems, and HCW improved skin integrity are variables to consider for HAI reduction. Automated touchless dispensing and closed-refill systems minimize touch points that help prevent cross contamination events and reduce dispenser contamination with clinically significant pathogens in healthcare settings. Additionally, most consumers prefer touchless dispensing when using public restrooms. However, many healthcare settings still utilize manual dispensers for soap and sanitizer. Some even have open, bulk, refillable dispensing systems and manual pumps and dispensers. A finding of a clinical in-use study revealed a significant reduction in the incidence of healthcare associated infections (HAIs) following the conversion to automated touchless soap and sanitizer dispensing systems with premeasured dosage amounts in a long-term care (LTC) care facility. A LTC facility was qualified as a testing site for clinical in-use study of hand hygiene (HH) products’ impact on healthcare workers’ (HCWs) hands. The facility replaced all HH manual dispensing systems and locally purchased hygiene products with automated touchless dispensers, closed-refill systems, and quality formulations. In addition to the biophysical measurements of HCWs hands using bioengineering instrumentation, the baseline incidence of HAIs was recorded prior to the intervention and surveillance continued from 2014 to 2018. HAI rates provided by the testing site were compared to the baseline data collected in 2014 with specific emphasis on the infection outbreak season from October to December in each year. Descriptive data analysis of HAI incidence rates revealed significant and sustained reduction in HAI rates from 6.7% in October through December of 2014 to 1.6 % the same period in 2015, 2.8 % in 2016 and 2.4 % in 2017. Converting the HH framework from manual dispensing to automated touchless dispensing with closed refill systems during a clinical-in-use study resulted in a sustained reduction of HAI rates over a 3-year period. Increased awareness of HH, touchless hygienic dispensing systems, and HCW improved skin integrity are variables to consider for HAI reduction.