Aim- To evaluate the removal of gutta percha by hand file system, rotary file system and reciprocating file system from root canals by using CBCT. Materials and Method: Root canals of 42 extracted human mandibular premolars were prepared using the ProTaper Universal rotary system and then obturated. Root filling quality was confirmed on CBCT images. Specimens were divided into 3 groups (n = 14) according to the system used for filling removal, group 1: H File, group 2: MTwo rotary retreatment file, and group 3: WaveOne file. Each specimen was sent for CBCT Analysis. Statistical Analysis Used: Evaluation of data was done statistically using One-way Analysis of Variance and Tukey’s post hoc test. Results: There was statistical difference in all the groups (P>0.05). All systems demonstrated presence of remnants of filling material. Mean reduction in the volume of GP after retreatment of Group I was 0.006mm3, Group II was 0.002mm3 Group III was 0.003mm3. Conclusion: The system responsible for a better removal of root canal filling material was the MTwo Retreatment file system, followed by the WaveOne system and H File.