Hand Emasculation Research Articles

Use of Cytoplasmic Male Sterility, Compared to Hand Emasculation, and Translocations for Chromosome Analysis in Sorghum vulgare Pers1

Results obtained after use of a cytoplasmic male‐sterile restorer gene system in testcrosses with translocations for chromosome analysis were compared with those from testcrosses after hand emasculation. The use of male‐sterile cytoplasm was relatively easy and less time consuming, thus allowing production of large numbers of progeny and an accurate estimate of linkage intensity.This variation in methodology may have application with other self‐pollinated crops where cytoplasmic male‐sterile restorer gene systems and transloeation stocks exist.Using this scheme the linkage groups T231(13.06±l.1)‐ Dd and T396(5.0±0.6)‐Yy were found, and the possible location of these markers was discussed. The establishment of the chromosome complement and male sterility of MSCK60 as a standard for chromosome analyses in sorghum was proposed.

Studies of chemical emasculation in pepper. I

In an attempt to eliminate the trouble of hand emasculation from the hybridization work in pepper, possibility of emasculation with a gametocide was examined. Mie Midori, a cultivar of sweet pepper, was used in this experiment. Anther dehiscence was completely suppressed by foliage spray of 250 and 1250 ppm aqueous solutions of sodium 2, 2-dichloropropionate (Dalapon). Suppression of anther dehiscence started about two weeks after treatment. Toxic effects to foliage and to plant growth were not severe while abnormal expansion of ovaries and bending of styles were accompanied with the suppression of anther dehiscence in a considerable number of flowers. The implications of these functional and morphological changes for hybrid seed production are discussed.

Effectiveness of Hand vs. Scissor Emasculation and Different‐Aged Pollen in Wheat Hybridization

Effectiveness of Hand vs. Scissor Emasculation and Different‐Aged Pollen in Wheat Hybridization