Abstract: BACKGROUND: Hamstring tightness is a common issue affecting individuals, leading to discomfort, pain, and increased risk of injury. The Bowen technique, a gentle myofascial release technique, and retro-walking have been suggested as potential interventions to improve hamstring flexibility and dynamic balance. This study aimed to assess the impact of combining the Bowen technique with retro-walking on hamstring flexibility and dynamic balance in college students. METHODOLOGY: A pre–post-experimental study was conducted among 50 college students aged 17–25 years. Participants were randomly allocated to either the experimental group (n = 25) receiving Bowen technique and retro-walking intervention or the control group (n = 25). Hamstring flexibility was assessed using the active knee extension test, and dynamic balance was evaluated using the Y-Balance test. The data were analyzed using paired and unpaired t-tests. RESULTS: Intragroup analysis revealed significant improvements in hamstring flexibility and dynamic balance posttreatment in the experimental group (P < 0.05), with no significant changes observed in the control group (P > 0.05). Intergroup analysis demonstrated statistically significant improvements in hamstring flexibility (P < 0.05) and dynamic balance (P < 0.05) between the experimental and control groups postintervention. DISCUSSION: The results indicate that the combination of the Bowen technique and retro-walking effectively improves hamstring flexibility and dynamic balance in college students. The Bowen technique likely enhances muscle extensibility and tissue repair, while retro-walking optimizes muscle length utilization and proprioception. These findings underscore the potential utility of these interventions in clinical practice for managing hamstring tightness and improving overall musculoskeletal health. CONCLUSION: The integration of the Bowen technique with retro-walking holds promise as a noninvasive and effective approach to enhance hamstring flexibility and dynamic balance in college students.