The Woodleigh multi-ring structure, buried by Cretaceous and, at its centre, Lower Jurassic lacustrine sediments, east of Hamelin Pool, Carnarvon Basin, Western Australia, is identified as an impact structure, the largest discovered to date on the Australian continent. An impact origin is indicated by: a central core of uplifted granitoid basement probably less than 25 km in diameter, which displays shock-induced planar deformation features in quartz, pervasive diaplectic vitrification of feldspar and penetrative pseudotachylite veining; and an inner ring syncline containing a ∼70 m thick thermally modified diamictite overlain by ∼380 m of Lower Jurassic lacustrine deposits. An outermost diameter of 120 km, defined by gravity, magnetic and surface drainage, indicates a ring fault that sharply intersects the NS-striking regional structure. At the centre of the basement uplift shock metamorphosed granitoid was intersected at a depth of 171 m, at least 1800 m higher than the gravity-modelled level of regional basement. Pseudotachylite vein systems within the shocked granitoid are strongly enriched in Al, Ca, Mg, Ni, Co, Cr, V and S, and depleted in K and Si, suggesting chemical fractionation attendant on shock volatilisation, enrichment by an injected and volatilised meteoritic component, and potentially of sulfide mineralisation. The impact age is constrained by overlying Lower Jurassic strata, reworked Early Permian palynomorphs in the Jurassic lacustrine section, and deformed Lower Devonian and older units. A regional thermal event identified by apatite fission track at 280–250 Ma hints at a possible Permian–Triassic boundary age for the impact, although the lack of Triassic fossils in the crater fill favours a late Triassic age.