Hamelia Patens Research Articles

RETRACTED: Green and efficient synthesis of cellulose nanocrystals from Hamelia patens leftover via hydrolysis of microwave assisted-ionic liquid (MWAIL) pretreated microcrystalline cellulose

The efficient bioconversion of the lignocellulosic agro-waste has immense importance in biorefinery processing in extracting the cellulose and saccharide fractions. To achieve this, a series of chemical pretreatments is employed, thus concerning environmental threats limit its use. Therefore, an ionic liquid is employed for pretreatment before sustainable extractions owing to its safe manipulation, recycling, and reusability. Specifically, microwave-assisted ionic liquid (MWAIL) pretreatment has significant importance in extracting high cellulose yield at less thermal power consumption. In this study, the leftover stalks of Hamelia patens were subjected to MWAIL pretreatment at 60, 70, 80, and 90 °C to extract microcrystalline cellulose (MCC). Subsequently, the MCC was fabricated into cellulose nanocrystals (CNC) through hydrolytic treatment using acidic and ionic liquids and denoted as CNC-AH and CNC-ILH. Thus obtained CNC was characterized by FTIR, FESEM, XRD, and TGA to investigate the influence of solvent on its morphology, crystallinity, and thermal stability of CNC. The results support that the CNC-ILH has comparatively more thermal and dispersal stability with a reduced crystallinity index than CNC-AH. The surprising results of CNC-ILH signify its utilization in diverse applications in the food and industrial sectors.

Induction of Monoterpenoid Oxindole Alkaloids Production and Related Biosynthetic Gene Expression in Response to Signaling Molecules in Hamelia patens Plant Cultures.

Hamelia patens (Rubiaceae), known as firebush, is a source of bioactive monoterpenoid oxindole alkaloids (MOAs) derived from monoterpenoid indole alkaloids (MIAs). With the aim of understanding the regulation of the biosynthesis of these specialized metabolites, micropropagated plants were elicited with jasmonic acid (JA) and salicylic acid (SA). The MOA production and MIA biosynthetic-related gene expression were evaluated over time. The production of MOAs was increased compared to the control up to 2-fold (41.3 mg g DW-1) at 72 h in JA-elicited plants and 2.5-fold (42.4 mg g DW-1) at 120 h in plants elicited with SA. The increment concurs with the increase in the expression levels of the genes HpaLAMT, HpaTDC, HpaSTR, HpaNPF2.9, HpaTHAS1, and HpaTHAS2. Interestingly, it was found that HpaSGD was downregulated in both treatments after 24 h but in the SA treatment at 120 h only was upregulated to 8-fold compared to the control. In this work, we present the results of MOA production in H. patens and discuss how JA and SA might be regulating the central biosynthetic steps that involve HpaSGD and HpaTHAS genes.

Volatile organic composition of five Rubiaceae species: Insights into their phytochemical diversity

Natural volatile organic compounds (VOCs) obtained from five Rubiaceae plants native to south China, namely Damnacanthus indicus, Hamelia patens, Exallage chrysotricha, Psychotria serpens, and Spermacoce pusilla, were isolated and characterized in this study. The analysis revealed that each plant contained 71, 57, 80, 73, and 70 volatile organic chemical compounds, respectively. The main VOCs shared by five plants was palmitate (10.20%–36.26%). Methyl salicylate, caryophyllene oxide, tetradecanoic acid, 6,10,14-trimethyl-2-pentadecanone, pentadecanoic acid, pentacosane, pentadecanal, nonanal, hexadecanoic acid methyl ester, phytol, linalool, and (E)-β-ionone were shared by at least 4 Rubiaceae plants in this study. Besides, each of the five plants has its main components as follows: 12.81% ethyl linoleate and 6.13% (E)-13-octadecenoic acid for D. indicus; 35.17% methyl salicylate for H. patens; 9.50% (Z, Z)-9,12-octadecadienoic acid and 7.56% endo-borneol for E. chrysotricha; 13.93% methyl salicylate for P. serpens; 6.19% humulene and 6.08% humulene oxide II for S. pusilla. This research provides insights into the phytochemical diversity and classification of Rubiaceae plants, laying a solid foundation for future natural product development in the Rubiaceae family.

Identifying pollution scavenging potential of perennial plants growing in Maitreyi College Campus, University of Delhi

Aim: Air Pollution Tolerance Index (APTI) represents the potential of a plant to combat air pollution. In the present study, APTI value of 77 perennials growing in the Maitreyi College Campus, New Delhi was calculated, and these plants were classified into tolerant and sensitive species. Methodology: APTI was calculated by assessing four physiological and biochemical parameters, pH, Relative Water Content (RWC), Total Chlorophyll (TCh), and Ascorbic Acid (AA). Results: The highest APTI was observed in Musa sp. (10.52) indicating it to be the most tolerant, while the lowest in Aegle marmelos (1.93), showing it to be the most sensitive for pollution. Results showed Musa sp., Salmelia sp., Terminalia arjuna, Murraya exotica, Hamelia patens, Ravenala madagascariensis, Ficus racemosa, Cascabela thevetia, Eugenia uniflora, Nyctanthes arbortristis to have good potential in reducing air pollution in a sustainable manner. Species such as Aegle marmelos, Plumeria rubra, Dracaena, Carissa carandas, Eucalyptus sp. were sensitive to air pollution. Interpretation: Analysis of results suggested the importance of APTI analysis in a campus for cataloging the pollution tolerant and sensitive plants. The pollution tolerant plants could be used for the green belt formation, while plants, which are sensitive to pollution can be used as an indicator of pollution. Moreover, mapping and compilation of information of trees (deciduous and evergreen) and shrubs growing in the campus helped in documenting the rich flora of the campus with pollution tolerance potential. Key words: Air Pollution, Air Pollution Tolerance Index, Air quality, Trees

Biosynthesis of kratom opioids.

Mitragynine, an analgesic alkaloid from the plant Mitragyna speciosa (kratom), offers a safer alternative to clinical opioids such as morphine, owing to its more favorable side effect profile. Although kratom has been traditionally used for stimulation and pain management in Southeast Asia, the mitragynine biosynthesis pathway has remained elusive. We embarked on a search for mitragynine biosynthetic genes from the transcriptomes of kratom and other members of the Rubiaceae family. We studied their functions invitro and invivo. Our investigations led to the identification of several reductases and an enol methyltransferase that forms a new clade within the SABATH methyltransferase family. Furthermore, we discovered a methyltransferase from Hamelia patens (firebush), which catalyzes the final step. With the tryptamine 4-hydroxylase from the psychedelic mushroom Psilocybe cubensis, we accomplished the four-step biosynthesis for mitragynine and its stereoisomer, speciogynine in both yeast and Escherichia coli when supplied with tryptamine and secologanin. Although we have yet to pinpoint the authentic hydroxylase and methyltransferase in kratom, our discovery completes the mitragynine biosynthesis. Through these breakthroughs, we achieved the microbial biosynthesis of kratom opioids for the first time. The remarkable enzyme promiscuity suggests the possibility of generating derivatives and analogs of kratom opioids in heterologous systems.

Primary and secondary metabolites identification in Hamelia patens leaves

The aim of the present study was to investigate the presence of phytochemicals in leaves extracts (aqueous, methanol, ethanol) of Hamelia patens, the selected medicinal plant. Ethanolic and methanolic (organic) extract showed good number of compounds compared to aqueous extracts. Phyto-chemical analysis of organic extracts of Hamelia patens has shown the biological compounds like protein and amino acids, carbohydrates, cardiac glycosides, tannin, phenol, alkaloids, steroids, terpenoids, flavonoides and saponin. Quinones, phlobatannins, coumarins, emodins and fatty acids were absent in both the aqueous and organic extracts.

Growth characteristics and post trimming regrowth potential of tropical and sub-tropical landscape hedge plants in response to spacing

Spacing of landscape plants is one of the factors determining the outcome of a design in terms of canopy formation which is one of the variables for aesthetic appearance in terms of shapes and forms. This study was conducted between September 2016 and September 2017, to determine the appropriate intra-row spacing for five plant species in hedge formation. The plant species used were Duranta erecta, Hamelia patens, Ficus retusa, Buxus sempervirens and Acalypha wilkesiana. Transplanting was done at intra-row spacing of 30, 40, 50 and 60cm and a constant inter-row spacing of 100cm. The experiment was a Randomized Complete Block Design in split plot arrangement with three replicates. Plant height, number of leaves, number of branches and canopy space, as indices of species suitability for use as hedge, were measured fortnightly. Plant regrowth and aesthetic characteristics were assessed after periodic trimming. H. patens and B. sempervirens species had the tallest plants. B. sempervirens and D. erecta produced the highest number of leaves while D. erecta and H. patens had the highest number of branches. D. erecta had significantly (p<0.05) the fastest post-trimming shoot regrowth rate relative to other species following the 1st and 2nd trimming (25 and 28 WAT respectively). The effect of spacing on shoot regrowth length was significant after the 3rd, 4th and 5th trimmings (31, 34 and 37 WAT respectively). The highest shoot regrowth height was recorded at 40cm spacing followed by 30cm intra row spacing. All species responded best at 50cm intra-row spacing for the number of branches and canopy space as well as early hedge formation. The study concluded that B. sempervirens was suitable for ideal hedging at 40cm intra-row spacing while A. wilkiesiana and H. patens were only suitable as informal hedges. Hence, B. sempervirens species is recommended as the ideal hedge plant choice based on its early regrowth after trimming, high leaf and branch production as well as overall speed in hedge formation at 31WAT.

The Ultrasound-Assisted Extraction of Polyphenols from Mexican Firecracker (Hamelia patens Jacq.): Evaluation of Bioactivities and Identification of Phytochemicals by HPLC-ESI-MS.

The objective of the present work was to optimize the extraction of phytochemicals from Hamelia patens Jacq. by ultrasound-assisted extraction. Taguchi L9 orthogonal array was used to evaluate the factors solid/liquid ratio (1:8, 1:12, and 1:16), extraction time (10, 20, and 30 min), and ethanol concentration (0, 35, and 70%). Total polyphenols were the response variable. Chromatographic fractionation using Amberlite XAD-16 was carried out and the total polyphenols, flavonoids, and condensed tannins were quantified. The redox potential, the reduction of the 2,2-diphenyl-1-picrylhydrazyl (DPPH), and the lipid oxidation inhibition were determined. Anti-bacterial activity was evaluated. The phytochemicals were identified by liquid chromatography coupled to mass spectrometry. Optimal extraction conditions were a solid/liquid ratio of 1:16, ethanol of 35%, and 10 min of ultrasound-assisted extraction. Maximum polyphenol content in the crude extract was 1689.976 ± 86.430 mg of gallic acid equivalents (GAE)/100 g of dried plant material. The purified fraction showed a total polyphenols content of 3552.84 ± 7.25 mg of GAE, flavonoids 1316.17 ± 0.27 mg of catechin equivalents, and condensed tannins 1694.87 ± 22.21 mg of procyanidin B1 equivalents, all per 100 g of purified fraction. Its redox potential was 553.93 ± 1.22 mV, reducing 63.08 ± 0.42% of DPPH radical and inhibiting 77.78 ± 2.78% of lipid oxidation. The polyphenols demonstrated antibacterial activity against Escherichia coli, Klebsiella pneumonia, and Enterococcus faecalis. The HPLC-ESI-MS analysis revealed the presence of coumarins, hydroxycinnamic acids, and flavonoids.

First record of the Sparkling-tailed Hummingbird (Tilmatura dupontii, Trochilidae) for Sierra de Huautla Biosphere Reserve, Morelos, Mexico

Primer registro de colibrí cola pinta (Tilmatura dupontii, Trochilidae) para la Reserva de la Biosfera Sierra de Huautla, Morelos, México.En agosto de 2005 observamos y fotografiamos un macho de colibrí cola pinta (Tilmatura dupontii) que forrajeaba sobre flores del arbusto Hamelia patens (Rubiaceae). Esta observación representa el primer registro de esta especie en la Reserva de la Biosfera Sierra de Huautla, en el sur de Morelos, México, ca. 50 km al sureste de registros anteriores en Morelos.

Performance of Dye-Sensitized Solar Cell using Hamelia Patens leaves as natural dye

Dye-Sensitized Solar Cells (DSSC), a 3rd generation solar cell has gained huge attention in the field of solar photovoltaics as a substitute for the silicon solar cell. The performance of the DSSC mainly depends on all the important components such as transparent conductive oxide substrate, cathode, photo-anode, dye, and electrolyte. Additionally, sensitizing dyes also perform a vital role in the absorption of light and the conversion of solar energy into electrical energy in DSSC. However, its efficiency is low and it consumes good amount of energy in manufacturing. In this respect scientists and researcher are more focus on how to improve its efficacy and production cost to make it economically viable. For this purpose, in this present work we have the natural dyes from Hamelia patens plant’s leaf which is available throughout the year for the fabrication of DSSC at low temperature. Semiconductor film electrode, ZnO has been synthesized via. chemical bath deposition process, which is anchored on the FTO glass substrate. Various characterizations techniques such as UV-vis absorption, FTIR were done for the extracted natural dye. XRD and FE-SEM were also performed for crystallinity and morphological analysis of ZnO material. XRD data reveals the formation of hexagonal ZnO (JCPDS card no. 01-082-9744 where 2θ° at 31.799, 34.454, 36.289, 47.586 corresponds to (100), (002), (011), (012) respectively). Using the ZnO and natural dyes, a sandwiched type DSSC has been fabricated and various parameter such as power conversion efficiency, Voc (open circuit voltage), Isc (short circuit current) has also been measured for continuous 3 days. DSSCs which is fabricated by using natural dye extracted from Hamelia patens leaves (sample 1) shows Voc = 132.2 and Isc = .065 mA.

Resource Partitioning in the Sunbird Species Found in Federal College of Forestry, Jos, Plateau State

Competition occurs when individuals or different species are vying for the same resource which is in limited supply. Two complete competitors cannot coexist indefinitely, therefore competitors must differ to some degree in their resource use. The research was carried to investigate how resources are partitioned among the species of sunbirds found in Federal College of Forestry, Jos. Opportunistic sightings along a 200m transect was used for observation. Sunbirds were observed using binoculars between 6:30 – 8am and by 4:30 – 6pm. Six transects were purposively selected in the study area. Sightings along a 200m transect was used to observe; species of sunbird, sex of individual sunbird species, species of flowering plant visited by the sunbird species, activity or behaviour performed by the sunbird (probing, insect hunting, roosting, perching etc.) and duration of activity. Descriptive statistics, ANOVA and post hoc test was used to analyse the data obtained. Results showed that forty-three (43) plant species were utilised by the four (4) sunbird species found in the study site with Corymbia torelliana and Hamelia patens having the highest number of visit. These four sunbird species observed were; Scarlet-chested sunbird, Variable sunbird, Green-headed sunbird and the Copper sunbird. Mean number of plants visited shows that Green-headed sunbird visited more plants than the other three species (28.89) which was highly significant (p=0.001). Copper sunbird spent the highest foraging time (67.71) which showed no variation with the other species (p=0.516). Females of the sunbird species visit more plants species and spent more time foraging as compared to the males although there was no variation (p= 0.984 and p= 0.906 respectively). The activities of the males (perching, hovering, probing, feeding) was higher than that of females and had a high level of significance (p=0.001). Callistemon citrinus, Corymbia torelliana, Eucalyptus camaldulensis, Hamelia patens, Parkia biglobosa and Ziziphus mucronata were utilised by all the sunbird species. Scarlet-chested sunbirds spent more time probing which aids pollination of plants in the study site and hence ecologically important to the plants found in the study site.

Beneficial Insects Visiting Florals of Hamelia patens Rubiaceae (Jacq.) at a University Landscape in Nigeria

Aim: The study was carried out to ascertain how Hamelia patens would be valuable in sustaining diversity of beneficial insects.
 Study Design: Investigative cross-sectional study.
 Place and Duration of Study: The study was carried out at University of Port Harcourt, Nigeria. The insects and plants were processed for identification at the Laboratories of the Department of Animal and Environmental Biology and Plant Science and Biotechnology, University of Port Harcourt, Nigeria. Identification and curation of the insects was done at Insect Museum, Ahmadu Bello University Zaria, Nigeria. The study started in June and ended in September 2018.
 Methodology: The heights and canopy sizes of the Hamelia patens were measured with range pole and measuring tape. Insects associated with the floral parts of Hamelia patens were collected in the morning (08:00-10:00 am) and in the evening (4:00-06:00 pm) hours, with a sweep net. They were knockdown by pyrethrum insecticide and preserved in a bottle containing 70% ethanol. They were taxonomically grouped and sent to a taxonomist at Insect Museum, Nigeria for species identification.
 Results: Fifteen (15) insect species were collected on the Hamelia patens; Megachile mephistrophelica (Grib.), Megachile cinta (Fab.), Braunisca bilunta (Enderloein.), Pterandus sp., Lilioceris sp. and Virachola antalus (Hoph.) restricted their visitation on the plants only in the morning hours, Chelonus bifoveolatus (Szepg.) and Chrysolagria nairobana (Borch.) restricted their visitation in the evening hours. The remaining species were continuous on the plants. There was no significant difference (P=.05) between the number of insect species collected on taller plants and shorter ones. There was a significant difference between the insects that visited the plants in the morning and evening hours.
 Conclusion: The arrival of the insects on the Hamelia patens varied but some were time dependent. The clipping of the plant’s twigs affected the abundance of insects that visit the plant.

Facile synthesis of self-assembling silver spheres and evaluation of their catalytic properties in organic dyes degradation

Hierarchical self-assembly nanostructures have an important interest in nanoscience and nanotechnology. This research reports a facile method for self-assembly of silver spheres using the Hamelia patens plant extract as a reducing agent. Furthermore, the degradation of organic dyes assesses the catalytic properties of Ag nanostructures. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), UV–Vis, and Fourier transform infrared techniques characterized the Ag spheres’ morphology and structure. SEM and TEM images revealed porous Ag assemblies with spherical morphologies of an average size of ~2 µm integrated by 20 nm thick petals. Also, more compact Ag spheres with an average size of around 3 µm, containing nanorods of around 100 nm on their surface, were obtained. Energy dispersive spectrometer and XRD techniques determined the chemical composition and crystal structure of the spheres. UV–Vis spectra show bands ranging from 400 to 500 nm, confirming quantum confinement in the material. Silver spheres with petals showed the best degradation performance of organic dyes such as methylene blue, methyl orange, and rhodamine B.

Novel anticancer drug delivery system based on zeolite encapsulating Hamelia patens leaf and flower extracts

Nanotechnology had placed an impact in improving the antiproliferative activity of crude leaf (CL) and flower (CF) extracts of Hamelia patens on the liver (HEPG2) and breast (MCF7) carcinoma cell lines, after their encapsulation onto zeolite (ZSM-5) nanopores, which acts as a drug delivery system (DDS). The cytotoxicity of the new formulas showed remarkable improvement in the activities on HEPG2. CL had low activity on HEPG2, at the tested concentrations, but the ZSM-5 loaded one (CLZ) showed enhanced action with IC50=42 μg/mL. Also, the cytotoxicity of CF improved after Zeolite incorporation, where the IC50 has been changed from 48 μg/mL for CF to 25 μg/mL for CFZ. Moreover, the polar fraction of leaves extracted by methanol (Me.L) showed improvement on the cytotoxicity on MCF7 for zeolite loaded formula (Me.Z) (IC50 had changed from 65 to 44.4 μg/mL). Quantitative estimations for polyphenolic contents as well as the phenolic profiles for CF and CL extracts were inspected using HPLC-DAD to explore the bioactive phytochemical compounds. Acute toxicity test showed that LD50 was 1500 and 2333 mg/kg b.wt. for CL and CF, respectively. The cytogenetic study was also done to detect the chromosome aberrations in somatic and germ cells after CL and CF administration to mice. DPPH antioxidant capacity assay revealed that CL has high redox-active effect than CF. This study is a contribution to the development of a creative new DDS that help in cancer treatment.

Preliminary phytochemical analysis and antibacterial study of crude extract from Hamelia patens stems

The present study aimed to analyse the phytochemical and antibacterial activity of different extracts of the tropical plant Hamelia patens Jacq. (Rubiaceae). The antimicrobial activity was carried out using agar well diffusion method. Hexane, petroleum ether, ethanol and chloroform stem extracts (100 μL of 50 mg/mL each) were introduced into the wells separately and allowed it to undergo incubation. After the incubation time was over, the plates were observed for zones of inhibition and compared with positive control ciprofloxacin at a concentration of 30 μM. These results provided evidence that the tested plant extracts possess antimicrobial properties which can be tested further in the development of novel antimicrobial agents.

Phytochemical, antimicrobial and antioxidant studies of the leaf extract of Hamelia patens

This study aimed to characterise the different extracts of the leaves of Hamelia patens Jacq. (Rubiaceae) and to evaluate their antibacterial and antioxidant activities in vitro. Phytochemical screening of the leaf extract revealed the presence of alkaloids, flavonoids, sterols, tannins and carotenoids. The extracts were further characterised with the help of FTIR and UV-VIS spectroscopy. The antibacterial activity was evaluated by agar well diffusion method and the zone of inhibition was measured. 1,1-diphenyl-2- picrylhydrazyl (DPPH) assay was used to evaluate the antioxidant capability of the extract. The extract was found effective in inhibiting the growth of both E. coli and S. aureus at 5 μg/mL concentration. The methanolic extract was also found to scavenge DPPH radicals. Although the extract is found effective in the present study, however, further research is required to evaluate the biological activity in higher experimental models.

A Phytochemical and Ethnopharmacological Recapitulation on Hamelia patens

The world health organization reports that 80% of the population living within the developing countries depends basically on traditional medicine for his or her primary health care. Quite half the entire world's population still depends entirely on plants for medicines, and plants provides the active ingredients to the most traditional medical products. Hamelia patens that belong to the family Rubiaceae, is mainly found in tropical and sub-tropical areas. It is used in folk medicine against a wide range of diseases such as athlete's foot, skin problems, insect sting, psychiatric disorder, rheumatism, headache, asthma, dysentery, menses, ovarian and uterine disorders. Hamelia consists of an important bioactive constituent such as oxindole alkaloids, flavonoids, and phenolic content. Due to the presence of chlorogenic acid and epicatechin constituent in the methanolic extract of Hamelia patens, there is a noticeable anti-hyperglycemic activity as well as it possesses antioxidant activity. Acute and sub-acute toxicity study has been performed on Hamelia patens which shows that it is safe and can be used for humans. Against fungi and bacteria, the ethanol leaf extract of Hamelia has a maximum inhibitory effect. The plant has various therapeutic effects like anti-inflammatory, analgesic, anti-diarrheal, anthelmintic, antidepressant, hepatoprotective, antiurolithiatic, diuretic, wound healing, and many others. In this article, we have discussed chemical constituent, pharmacological activity and traditional use of Hamelia patens.

Biodiversity of pollinators in four bee-friendly plant species

Bees are the primary pollinators of many important agricultural crops. Enhancing the suitability of farm landscapes for native pollinators by growing flowering non crop plants is necessary for in-situ conservation of bee pollinators. A study has been conducted to find the role of four different plants, viz., Hamelia patens, Ocimum basilicum, Asystesia sp. and Jacquemontia sp. in the conservation of native bee pollinators. The different species of bees visiting the flowers were Apis cerana, A. florea, Hoplonomia sp., Amegilla zonata, A. confusa and Ceratina hieroglyphica . The diversity indices were higher during morning hours than the afternoon. The number of bees visited per flower, time spent and numbers of flowers visited on Jacquemontia sp. were more compared to other plant species. Biodiversity indices were calculated by using Insect Biodiversity Analysis Portal, which is an online tool to carry out biodiversity analysis and hosted at https://www. nbair.res.in/Biodiversity. The planting of bee-friendly plant species as identified in this study will help support healthy, diverse pollinator and other beneficial insect communities.

Propagation and seedling growth of some species used as ornamental hedges in landscape design

Propagation and seedling growth characteristics of five plant species: Duranta repens (Yellow bush), Hamelia patens (Fire bush), Ficus retusa (Yellow ficus), Buxus sempervirens (Boxwood / West indies) and Acalypha wilkesiana (Copper leaf), were examined in the nursery as indices of suitability for use as hedge plants in the landscape. The experiment was laid out in a Completely Randomized Design (CRD) with four replicates where the treatments were the plant species. Cuttings were cultivated in polyethylene pots filled with top soil medium. Data collected included: time to rooting, rooting percentage, number of leaves/plant, seedling height and stem girth. They were subjected to analysis of variance and means separated using Least Significant Difference (LSD) at 5% probability level. It took Acalypha wilkesiana , Duranta repens and Hamelia patens species shortest time (21 days) to root while Buxus sempervirens and Ficus retusa took the longest time (28 days) for rooting. Duranta repens species produced the highest rooting percentage (80.5%) at 28 days after planting and the highest number of leaves/plant (15leaves). This study revealed Duranta repens as the easiest to propagate among these species. All plant species evaluated except Ficus retusa are easily propagated in the nursery thus indicating their potential suitability for commercial production in the nursery and use as hedge plants. Duranta repens and Buxus sempervirens are recommended as hedge plant choices based on their ease of propagation and early establishment.

Green Synthesis and Characterization of Ag@Au Core-shell Bimetallic Nanoparticles using the Extract of Hamelia patens Plant

Journal Article Green Synthesis and Characterization of Ag@Au Core-shell Bimetallic Nanoparticles using the Extract of Hamelia patens Plant Get access K Chavez, K Chavez Instituto de investigación en Metalurgia y Materiales, UMSNH, Morelia, México Corresponding author: kchavez@umich.mx Search for other works by this author on: Oxford Academic Google Scholar G Rosas G Rosas Instituto de investigación en Metalurgia y Materiales, UMSNH, Morelia, México Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 25, Issue S2, 1 August 2019, Pages 1102–1103, https://doi.org/10.1017/S143192761900624X Published: 01 August 2019