Hair Growth Activity Research Articles

SOLID LIPID NANOPARTICLES OF FENUGREEK SEED EXTRACT IN A DERMATOLOGICAL BASE FOR ALOPECIA: AN IN VIVO STUDY

Objective: The objective of the study was to investigate the potential of Solid Lipid Nanoparticles (SLN) of Fenugreek Seed Extract (FSE) in a dermatological base for its hair growth activity in alopecia Methods: The optimized SLN formulation of FSE was loaded into neutralized Carbopol 934 gel, and its hair growth efficacy was studied in Wistar rats in terms of hair density and length. Alopecia was induced in the rats by administering cyclophosphamide at a dose of 40 mg/kg for three days. The formulations were applied to the skin for twenty-one days following the induction of the disease. The hair growth in FSE-SLN gel-treated groups were compared with disease control and other treatment groups using qualitative and quantitative assessments. Results: FSE-SLN gel reduced the hair growth completion time comparable to that of the standard (p<0.01). The increase in hair length was significantly (p<0.01) greater in FSE SLN groups compared to groups treated with conventional gels, oils, and marketed formulations, demonstrating the superior hair growth efficacy of the developed FSE SLN. Conclusion: SLNs can enhance the penetration of extract into the skin (stratum corneum) compared to oil and gels, thereby increasing treatment efficiency, targeting the epidermis, and reducing systemic absorption and related side effects. Consequently, the developed nanoformulation can be a substitute for in vivo hair growth activity.

Hair Growth Effect and the Mechanisms of Rosa rugosa Extract in DHT-Induced Alopecia Mice Model.

Rosa rugosa is a medicinal plant known for its potential anti-inflammatory, antioxidant, anti-cancer, and antimicrobial benefits. The pharmacological effects of Rosa rugosa extract on hair loss have not yet been documented. This research sought to assess the inhibitory effects and mechanisms of action of Rosa rugosa water extract (RWE) in a mouse model of dihydrotestosterone (DHT)-induced alopecia. The study was conducted using C57BL/6 mice, which were assigned to five groups: control, DHT-treated, Rosa rugosa water extract (RWE) at doses of 25 mg/kg and 100 mg/kg body weight, and bicalutamide-treated. To induce hair loss, dihydrotestosterone (1 mg/day per body weight) was administered via intraperitoneal injections, and dorsal hair removal was timed to align with the telogen phase. Each group received oral treatments for a period of 23 days. In this study, we assessed hair growth activity, examined histological changes, and performed immunoblot analysis. We noted improvements in hair length and thickness. Additionally, the protein expression of growth factors associated with hair growth, including vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1), showed significant increases in the group treated with RWE. Additionally, treatment with RWE suppressed the protein expression of hair growth inhibitory factors, including dickkopf WNT signaling pathway inhibitor 1 (DKK1) and interleukin (IL)-6. Moreover, hair growth regulatory pathway related factors, including ERK, AKT, and GSK-3β, were activated. These findings indicate that RWE could serve as a promising natural therapy for preventing hair loss by enhancing the production of factors that promote hair growth while inhibiting those that suppress it.

Formulasi Hair Tonic Ekstrak Etanol Kulit Buah Pepaya (Carica Papaya L.) dan Uji Efektivitas Pertumbuhan Rambut pada Marmut Jantan (Cavia porcellus)

Hair for humans has an important role, in addition to protecting the skin from adverse environments, hair also plays an important role in a person's appearance. Losing a lot of hair due to hair loss can cause a lack of self-confidence, disappointment, and frustration, so it must be addressed immediately so as not to worsen the condition that can cause baldness. This study aims to determine whether there is a content of secondary metabolite compounds in fresh herbal medicine, herbal medicine powder and ethanol extract of papaya fruit skin; ethanol extract of papaya fruit skin (Carica papaya L.) can be formulated in the form of hair tonic preparations; whether the difference in concentration of ethanol extract of papaya fruit skin (Carica papaya L.) provides different activities in triggering hair growth in guinea pigs. This research method uses a quantitative approach and is experimental with independent variables, namely papaya fruit skin, herbal medicine, and ethanol extract of papaya fruit skin with concentrations of 10%, 20%, 30%. Research stages: phytochemical screening of fresh papaya fruit peel, powdered simplicia and ethanol extract, hair tonic formulation containing ethanol extract of papaya fruit peel 10%, 20% and 30%, hair tonic evaluation includes: organoleptic, viscosity, stability, pH, irritation, and preference test. And hair growth activity test on male guinea pigs. The results showed that the ethanol extract of papaya fruit peel contains alkaloids, flavonoids, saponins, steroids, and glycosides, can be formulated into hair tonic (EEKP) that meets physical quality requirements. Hair tonic EEKP 30% is the best because it has high hair growth activity, which is an average of 16.375 mm where this EEKP 30% hair tonic has almost the same hair growth activity as the positive control, which is an average of 16.782 mm.

HAIR TONIC OF BROCCOLI (BRASSICA OLERACEA VAR. ITALICA) EXTRACT: FORMULATION, PHYSICAL CHARACTERISTIC, STABILITY AND HAIR GROWTH ACTIVITY TEST

Objective: Hair loss is a common scalp concern for both men and women. Broccoli (Brassica oleracea var. Italica) is a vegetable plant in the cabbage family or brassicaceae that has antioxidant activity and levels of flavonoids that can encourage hair growth. Hair tonic is one of cosmetic products that contain ingredients to nourish hair. The purpose of this study was to investigate the physical stability and activity of hair development in hair tonic preparations using broccoli stalks extracted at different concentrations of 30%, 35%, and 40%, followed by an examination of the hair growth activity test in an animal model. Methods: This study hair tonic was prepared with Broccoli (Brassica oleracea var. Italica), the sample used the broccoli stalks, which was taken by purposive sampling. Evaluation of the preparation includes physical characteristic observation, pH examination, homogeneity of the preparation, irritation test, and stability test of the preparation using a cycling test, and check the activity test of hair growth in rats’ skin. Results: The results showed that the hair tonic preparation containing broccoli stalks extracts was stable at a temperature (4 °C±40 °C) where the odor, color, shape, homogeneity, and pH remained constant. In statistical testing using ANOVA analysis, each group of the hair tonic formula of broccoli stalks extract showed hair growth activity in male white rats. The hair tonic formulation containing 40% broccoli stalks extract had better hair growth activity compared to the 30% and 35% formula groups. The hair tonic prepared from broccoli extract is confirmed safe for use after successfully passing the skin irritation test. Conclusion: The hair tonic of broccoli extracts had met the standard of physical characteristics during storage time and it has a good activity test on hair growth in male white rats. This study provides a novel formulation in hair growth.

FORMULATION OF HAIR TONIC COMBINATION OF ETHANOL EXTRACTS OF MORINGA OLEIFERA LAM LEAVES AND FRAGRANT PANDAN (Pandanus Amaryllifolius Roxb) AND ITS ACTIVITY ON HAIR GROWTH IN WHITE RATS

Hair is the crown for everyone because hair functions aside from providing warmth, protection, beauty, and support. Hair loss that can cause baldness is one of the most serious problems for everyone. This study aimed to determine the activity of Hair Tonic combination formulations of kelor leaf ethanol extract (Moringa oleifera Lam.) and ethanol extracts of Fragrant Pandan leaves (Pandanus amaryllifolius Roxb) on rat hair growth compared to single use. The method began with the maseration process of ethanol extract of kelor leaves and ethanol extract of Fragrant Pandan leaves, characterization of extracts (specific and non-specific parameters), chemical content testing of extracts and formulation of Hair Tonic preparations consisting of F1 (positive control), F2 (6% kelor leaf extract), F3 (6% ethanol pandan extract), F4 (combination of 2% kelor leaf extract with 4% ethanol pandan extract), F5 (3% kelor leaf ethanol extract and 3% ethanol pandan leaf extract), F6 (combination of 4% kelor leaf ethanol extract and 2% ethanol pandan leaf extract). The rat hair growth activity was divided into 8 groups: each rat's back was shaved to a size of 4 × 4 cm, the rats were left for 24 hours and then testing was carried out by spraying each formula, and hair growth was observed for 28 days. ...

RoPod, a customizable toolkit for non-invasive root imaging, reveals cell type-specific dynamics of plant autophagy

Arabidopsis root is a classic model system in plant cell and molecular biology. The sensitivity of plant roots to local environmental perturbation challenges data reproducibility and incentivizes further optimization of imaging and phenotyping tools. Here we present RoPod, an easy-to-use toolkit for low-stress live time-lapse imaging of Arabidopsis roots. RoPod comprises a dedicated protocol for plant cultivation and a customizable 3D-printed vessel with integrated microscopy-grade glass that serves simultaneously as a growth and imaging chamber. RoPod reduces impact of sample handling, preserves live samples for prolonged imaging sessions, and facilitates application of treatments during image acquisition. We describe a protocol for RoPods fabrication and provide illustrative application pipelines for monitoring root hair growth and autophagic activity. Furthermore, we showcase how the use of RoPods advanced our understanding of plant autophagy, a major catabolic pathway and a key player in plant fitness. Specifically, we obtained fine time resolution for autophagy response to commonly used chemical modulators of the pathway and revealed previously overlooked cell type-specific changes in the autophagy response. These results will aid a deeper understanding of the physiological role of autophagy and provide valuable guidelines for choosing sampling time during end-point assays currently employed in plant autophagy research.

Validation and responsiveness of the English version of the Chemotherapy-Induced Alopecia Distress Scale (CADS) in breast cancer patients

PurposeThis study aimed to validate the Chemotherapy-Induced Alopecia Distress Scale (CADS) in a diverse English-speaking population and patients with endocrine treatment-induced alopecia (EIA).ObjectiveChemotherapy and endocrine therapy commonly cause alopecia in breast cancer patients, leading to significant psychological and social challenges. The CADS was developed to assess the psychosocial impact of alopecia, but its generalizability beyond Korean patients requires further investigation.MethodsData from the CHANCE study (NCT02530177), which focused on non-metastatic breast cancer, was used. The cohort included 256 patients, and CADS data were collected at baseline, 6 months after chemotherapy completion, or 12 months after initiating endocrine therapy. The CADS questionnaire comprised 17 items covering physical and emotional health, daily activities, and relationships. Reliability was assessed using Cronbach’s alpha, and responsiveness was measured by effect size.ResultsThe CADS exhibited good reliability, with Cronbach’s alpha of 0.91 for the overall score, indicating acceptable internal consistency in both chemotherapy (0.89) and endocrine therapy (0.86) groups. Longitudinal responsiveness was supported by an effect size of 0.49 between decreasing satisfaction with hair growth and increasing emotional distress. Cross-sectional validity was confirmed, with effect sizes of 0.91 and 0.92 for satisfaction with hair growth and emotional and activity domains, respectively.ConclusionThe CADS is a valid and responsive tool for assessing the psychosocial impact of chemotherapy-induced alopecia and endocrine treatment-induced alopecia in a diverse Western patient population.

Formulasi Sediaan Pomade Ekstrak Daun Meniran (Phyllanthus niruri L.) Sebagai Perangsang Penumbuh Rambut Pada Kelinci

Background: Meniran petroleum ether extract 2% which contains lignans and terpenoids can increase hair growth activity by inhibiting 5α-reductase. In addition, the ethanol extract of candlenut seeds from the same family as meniran Flavonoids and phenols can also stimulate hair growth. Previous studies have also shown that quercetin also has activity to increase hair growth. Meniran is also known to contain the flavonoids quercetin, nirurin, rutin, quercitrin, astragalin and galocatechin. So meniran ethanol extract was chosen for this study. Based on a literature search about this research, the researcher was interested in formulating meniran leaf extract pomade (Phyllanthus niruri L.) as a hair stimulant in rabbits (Oryclolagus cunucilus). Purpose: To determine the formulation of meniran leaf extract pmade (Phyllanthus niruri L.) which is good in stimulating hair growth in rabbits. Methods: The method used was an experimental study to test the characteristics of pomade preparations with meniran leaf extract concentrations of 5%, 7.5% and 10% with organoleptic, homogeneity, spreadability, pH, irritation and freeze-thaw parameters. Results: From the results of the research on the characteristic test of pomade preparations on formulations of formula I, formula II and formula III it was found that they fulfilled the characteristics of pomade while F0 did not meet the spreadability requirements. Conclusion: Among the three formulations, it was found that pomade with formula III had the effectiveness of a good hair growth stimulant and could compete with the positive control.

Optimization of Bear Oil Extraction Process and Hair Growth Activity.

According to ancient Chinese books, bear grease has the effects of strengthening muscles and bones, which is beneficial for weakness, but there is relatively little research on it. Thus, the extraction of it is beneficial for compensating for research in this area. In this study, a uniform experimental design method was used to optimize the extraction process of bear grease by enzymatic hydrolysis extraction, and the extraction rate can reach 81.89% under optimized extraction conditions. Furthermore, the components of bear grease obtained by this study were analyzed by GC-MS, and the results showed that ursolic oil was rich in unsaturated fatty acids (67.51%), which was higher than that of the traditional method (66.92%). The composition of bear grease extracted by the enzymatic method was also better than that extracted by the traditional method. In addition, bear grease obtained in this study had the obvious activity of promoting hair growth. The length, weight, and number of hair follicles in the depilation area of mice in the high-dose group were significantly different from those in the blank group (p < 0.01). This study optimized the extraction process of bear grease and conducted a preliminary analysis of its fatty acid composition, which is expected to provide some reference for the development of the medicinal value of bear grease.

Preparation of herbal hair oil exploring the therapeutic benefits of herbs and its evaluation

Background: Herbal cosmetics, particularly herbal hair oils, have gained popularity due to their perceived efficacy and minimal adverse effects. This study aimed to formulate and evaluate herbal hair oils for promoting hair growth. Methodology: Various herbal extracts, including Coconut, Curry leaves, Amla, Fenugreek, and Onion, were prepared and incorporated into virgin Coconut oil. Phytochemical screening, total flavonoid content, organoleptic evaluation, chemical analysis (acid value, saponification value, specific gravity), stability studies, antioxidant assay (DPPH), antimicrobial assay, sensitivity studies, and in-vivo hair growth activity were conducted. Results: Organoleptic evaluation revealed characteristic odors and colors across formulations. Chemical analysis showed acceptable values for acid value (0.68 - 1.86 mg KOH/g), saponification value (117.87 - 224.27 mg KOH/g), and specific gravity (0.865 - 0.933 g/cm^3). Stability studies over 45 days demonstrated consistent physicochemical properties. The DPPH assay indicated dose-dependent antioxidant activity, with inhibition ranging from 13.8% to 66.5%. Antimicrobial assay showed inhibition zones ranging from 6 mm to 20 mm against Staphylococcus aureus. Sensitivity studies exhibited no adverse reactions. In-vivo hair growth activity demonstrated significant improvements compared to controls. Discussion: The formulated herbal hair oils exhibited promising physicochemical properties, antioxidant activity, antimicrobial efficacy, safety, and hair growth promotion in animal models. These findings suggest their potential as natural remedies for hair care. Conclusion: Herbal hair oils formulated in this study show significant potential for promoting hair growth and addressing hair-related concerns. Whereas, formulation F3 shown significant efficacy across multiple parameters among the formulations. Further research and clinical trials are warranted to validate their efficacy and safety for human use.

Cinnamic acid promotes elongation of hair peg-like sprouting in hair follicle organoids via oxytocin receptor activation

Considerable global demand exists for the development of novel drugs for the treatment of alopecia. A recent report demonstrated that oxytocin promotes hair growth activity in human dermal papilla (DP) cells; however, its application in drugs or cosmetic products is challenging because rapid degradation and relatively large molecular weight prevent long-term topical administration on the scalp. Here, we examined cinnamic acid, a small molecule activator for oxytocin receptor (OXTR) expression. Treatment with cinnamic acid led to upregulation of OXTR and trichogenic gene expression in human DP cells. Furthermore, inhibition of OXTR with an antagonist, L-371,257, suppressed hair growth-related gene expression in DP cells. These findings suggest that cinnamic acid enhances the hair growth ability of DP cells via oxytocin signaling. Additionally, we tested the hair growth-promoting effects of cinnamic acid using hair follicle organoids in vitro and observed that cinnamic acid significantly promoted the growth of hair peg-like sprouting. These promising results may be useful for developing hair growth-promoting products targeting oxytocin.

Formulation and In-vivo Assessment of Topical Polyherbal Hair Serum to Promote Hair Growth

Male pattern baldness, or androgenic alopecia, is a term used to describe hair loss that results from the underlying sensitivity of hair follicles to androgenic shrinkage. Alopecia is caused by environmental factors, hormone imbalances, and/or inherited factors, according to research studies, however, many etiologies are still unclear. The purpose of treating androgenic alopecia is to slow down the thinning of hair and promote hair growth. A small number of natural treatments, along from allopathic medicines, have demonstrated effectiveness in the decades-long combat over alopecia. The main object of the present study was to develop and in-vivo evaluation of hair growth serum. Crude herbs extract of Hibiscus rosa-sinesis, Glycyrrhizza glabra, Eclipta alba, Withania somnifera and Bacopa monnieria were specifically weighed to make polyherbal serum and animal models are rats with induced androgen test to access for hair growth promoting activity. To assess the serum’s potential against alopecia, it was applied topically. Testosterone had been administered subcutaneously for 20 days to cause alopecia in albino rats. Serums made of herbal extracts were administered concurrently to prevent androgen from having the ability to cause baldness. A positive control was provided with finasteride. At the conclusion hair growth activity was assessed visually, follicular density was measured using an ocular micrometer, and the anagen/telogen ratio was measured using an animal skin sample.

Nourishing Hair Oil

Hair plays a very important role in the personality of humans and for their cure by using lots of cosmetic products. Herbal formulations always have activity and comparatively lesser or no side effects with synthetic. Hair formulation of Embica officinalis (Fruits), Bacopa monnieri (Leaves), Trigonella foenum graecum (Seeds), Murraya koenigii (Leaf), Hibiscus rosasinensis (Flowers) in different concentrations in the form of herbal oil were studied for their hair growth activity, refractive index, acid value, saponification value.. Admirable results of hair growth were seen in formulation prepared by different methods of oils preparation technique.

Hair Growth Activity of Hair Tonic Preparations Containing Cinnamon Essential Oil (Cinnamomum burmanii) and Virgin Coconut Oil

Cinnamaldehyde in cinnamon essential oil, lauric acid, myristic acid, and oleic acid in virgin coconut oil (VCO) were used in hair tonic preparations as hair growth agents. This study aimed to evaluate particle size and obtain optimum formulation of the hair tonic preparation and to observe growth activity with various levels of cinnamon essential oil and VCO. This research an experimental with rabbit used 4 test were divided into 6 treatment groups: 1) positive control (Regrou®); 2) normal control (without treatment); 3) negative control (hair tonic basis); 4) F1(1% cinnamon essential oil: 2.5% VCO); 5), F2 (3% cinnamon essential oil : 2.5% VCO); and 6), F3 (5% cinnamon essential oil: 2.5% VCO). The particle size analysis selected in formula 2 that cycle stable during storage, and fall into the microemulsion size range of 20-200 nm and there was no difference cycle because the significance was &gt;0.05. The hair growth activity was determined using Average Growth Daily Gain.(AGD) formula and then.was analyzed.statistically using.One Way Anova. The results showed that had hair growth activity that was not significantly different. However, when compared with the positive control, formula 3 has hair growth activity above the positive control.

Formulation and Evaluation of Polyherbal Lotus Oil

Herbal formulations always have attracted considerable attention because of their good activity and comparatively lesser or nil side effects with synthetic drugs. The objective of present study involves preparation of herbal hair oil using amla, hibiscus, bhringraj, methi and its evaluation for increase in hair growth activity. Each drug was tested for their hair growth activity in a concentration range for 1-10% separately. Based on these results mixture of crude drugs fruits of Embelica officinalis, flowers of Hibiscus rosa sinensis, leaves of Bacopa monnieri and seeds of Trigonella techniques (direct boiling, paste and cloth method) and were tested for hair growth activity. The oil of different concentrations were characterized for proximate analysis including moisture content, total ash, acid insoluble ash, water soluble ash, water insoluble ash, sulphated ash. The formulations were also subjected to chromatographic determination and chemical tests to determine the presence of active constituents in the drugs. But looking towards the formulation viscosity the maximum concentration of combined drug was found to be 30% at their maximum level. The formulation containing 7.5% of each drug used for the study and showed excellent hair growth activity with standard (2% minoxidil ethanolic solution) by an enlargement of follicular size and prolongation of the anagen phase. It holds the promise of potent herbal alternative for minoxidil. Excellent results of hair growth were seen in formulation prepared by boiling method of oils preparation technique.

FORMULASI SEDIAAN HAIR TONIC EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lamk.) DAN DAUN RAMBUTAN (Nephelium lappaceaum L.) TERHADAP PERTUMBUHAN RAMBUT

Moringa olifera lamk and Nephelium lappaceaum L leaves are empirically widely used to stimulate hair growth. The purpose of this research is to combine the kelor leaf extract and rambutan leaf extract formulated in hair tonic. The hair tonic preparations made were evaluated by performing a physical test of the preparation and knowing the best concentration of kelor leaf extract and rambutan leaf extract on rabbit hair growth. The hair tonic were orepared in 3 formulations with the ratio of kelor leaf exract and rambutan leaf extract (1:3 , 1:1 and 3:1)% w/v. the positive control used is hair tonic Minoxidil 2%. Physical tests of the preparation include organoleptic test, pH test, homogeneity test and Viscosity test. Hair growth activity on the 3 rabbits shaved hair and hair growth measurements until the 28 st day. The data of growth measurements were tested statistically by ANOVA test metode. Based on the reseach result, in can be concluded that hair tonic combination of kelor leaf extract and rambutan leaf extract with ratio (1:1)%, has average hair length 3 cm and hair weight 0,236 g is the optimum concentration in stimulating hair growth aktivity with positive control has average hair length 2,025 cm and hair weigth 0,207 g. Test statistically there was no significant difference between extract and positif control (P &lt; 0,05).

Boosting hair growth through follicular delivery of Melatonin through lecithin-enhanced Pickering emulsion stabilized by chitosan-dextran nanoparticles in testosterone induced androgenic alopecia rat model

The strategy in this work was loading Melatonin (MEL), the powerful antioxidant photosensitive molecule, in novel Pickering emulsions (PEs) stabilized by chitosan-dextran sulphate nanoparticles (CS-DS NPs) and enhanced by lecithin, for treatment of androgenic alopecia (AGA). Biodegradable CS-DS NPs dispersion was prepared by polyelectrolyte complexation and optimized for PEs stabilization. PEs were characterized for droplet size, zeta potential, morphology, photostability and antioxidant activity. Ex-vivo permeation study through rat full thickness skin was conducted with optimized formula. Differential tape stripping trailed by cyanoacrylate skin surface biopsy was executed, for quantifying MEL in skin compartments and hair follicles. In-vivo evaluation of MEL PE hair growth activity was performed on testosterone induced AGA rat model. Visual inspection followed by anagen to telogen phase ratio (A/T) and histopathological examinations were conducted and compared with marketed 5% minoxidil spray “Rogaine ®”. Data showed that PE improved MEL antioxidant activity and photostability. Ex-vivo results displayed MEL PE high follicular deposition. In-vivo study demonstrated that MEL PE treated testosterone induced AGA rat group, restored hair loss and produced maximum hair regeneration along with prolonged anagen phase amongst tested groups. The histopathological examination revealed that MEL PE prolonged anagen stage, increased follicular density and A/T ratio by 1.5-fold. The results suggested that lecithin-enhanced PE stabilized by CS-DS NPs was found to be an effective approach to enhance photostability, antioxidant activity and follicular delivery of MEL. Thus, MEL-loaded PE could be a promising competitor to commercially marketed Minoxidil for treatment of AGA.

Protective Activity against Oxidative Stress in Dermal Papillae with Extracted Herbal Essential Oils

Dermal papilla cells (DPCs) are a rich source of nutrients and secrete multiple growth factors that can affect hair growth. As oxidative stress leads to hair loss in humans, it is considered to be one of the factors that can impair the function of DPCs. Herb-derived phytochemicals exhibit potent antioxidant activities; therefore, this study investigated whether a set of essential oils (lavender, lemongrass, rosemary, and chamomile oils) promote the hair-growth activity of DPCs. Intracellular reactive oxygen species (ROS) increased markedly in ultraviolet B-irradiated DPCs (50 mJ/cm2) and were efficiently blocked by essential oils. Essential oils upregulated the mRNA and protein levels of phase II enzymes (detoxifying and antioxidant), including heme oxygenase-1, NAD(P)H quinone oxidoreductase-1, and glutathione S-transferase pi. They also upregulated and activated nuclear factor E2-related factor 2, an essential transcription factor for phase II enzymes. Regarding biomarkers for hair growth, essential oils significantly increased vascular endothelial cell growth factor and insulin-like growth factor-1 mRNA levels. In conclusion, phytochemicals in essential oils enhance hair growth through ROS-scavenging activity in DPCs.

The activity of candlenut oil in the nanostructured lipid carrier system on hair growth in rats.

Candlenut oil (Aleurites moluccana L. Willd), which is also called Aleurites moluccana Seed (AMS) oil, is empirically effective as a hair growth agent, it is an unstable substance. Nanostructured Lipid Carrier (NLC) is a delivery system that is proven to increase the effectiveness and stability of the material, and the usage of solid lipid combination: beeswax-oleum cacao can produce good NLC characteristics. To determine the effectiveness of NLC_AMS oil with different combination of beeswax-oleum cacao (100:0; 50:50; 25:75; and 0:100) as a hair growth agent, using rats as subjects. NLC-AMS oil was made using 20% of total lipid with 5% AMS oil as liquid lipid and 15% solid lipid; combinations of beeswax-oleum cacao were of different ratios (100:0; 50:50; 25:75; and 0:100). NLC was made by High Shear Homogenization (HPH) method. Hair growth activity test carried out on male white rats using the research methods of Yoon (2010). The addition of oleum cacao as a solid lipid did not affect the pH, but increased the consistency and decreased the particle size of NLC- AMS oil. There was a relationship between the characteristic of NLC and the hair growth activity test: the small particle size and low viscosity had greater hair growth activity. The usage of solid lipid combination: beeswaxoleum cacao can produce better NLC characteristics and had higher hair growth activity than the formulas that used single lipid.

Hair Growth Test in Male Rabbits (Oryctolagus cuniculus) With Variations in The Concentration of Ethanol Extract Terentang (Campnosperma brevipetiolatum Volkens) Stem Barks

Terentang (Campnosperma brevipetiolatum Volkens) stem bark contains alkaloids, saponins, tannins, and flavonoids that can nourish hair and blacken and clean hair. This study aimed to determine male rabbits' hair growth activity from terentang stem bark extract and the most effective concentration in hair growth. This study used 6 treatments, namely: no treatment, negative control (aqua dest), positive control (Regrou), and terentang stem bark extract with a concentration of 0.5%, 1%, and 5%. Treatment was carried out every day with an application volume of 1 ml for 21 days. Hair length measurements were carried out on the 7th, 14th, and 21st days. Hair weight measurements were carried out on the 22nd day. Data obtained were processed statistically with the one-way ANOVA method. The results of one-way ANOVA statistical analysis showed significant differences in hair growth activity in each treatment with a value of P=&lt;0.00 (P&lt;0.05). Based on the results, it can be concluded that terentang stem bark extract with a concentration of 5% was the most effective concentration in growing hair of male rabbits.