In 4 cats the electroencephalographic changes recorded in the cortex, the thalamus and the mesencephalic reticular formation during conditioning were studied. Conditioning was produced by coupling of an unconditioned light stimulus in the form of intermittent photic stimulation with a preceding conditional auditory stimulus in the form of a continuous sound. This study allows to draw the following conclusions: 1. 1. The results confirm certain findings obtained in investigations carried out in the recent years. The appearance of evoked potentials and of a desynchronization during the presentation of the conditioned stimulus characterizes the first stage of conditioning. During a second stage the desynchronization is replaced by hypersynchronous repetitive discharges which progressively tend to follow the frequency of the intermittent photic stimulation. The so-called stage of experimental neurosis is characterized by the alternation of rapid patterns with sleep patterns during which the conditioned electroencephalographic responses characterized by desynchronization or by specific rhythmic discharges disappear. 2. 2. The role played by the reticular formation in the elaboration of conditioning processes is again stressed: 3. (a) the conditioned specific responses are of earlier onset, higher amplitude and are more stable in the reticular formation; 4. (b) the daily repetition of the experiments progressively increases the relationships of phase, form and amplitude between the bursts and other electrographic elements recorded in the occipital cortex and in the reticular formation without any stimulation. 5. 3. A new finding was obtained in this, study: the spontaneous appearance without any stimulation of repetitive discharges at the frequency of the photic flashes and almost exclusively recorded from the reticular formation. These rhythmic discharges appear as soon as the animal is placed in the habitual conditions of the experiment. They could represent an elementary electrographic manifestation of a memory processes.
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