An assessment to determine habitat selection by large mammals was carried out in South Luangwa National Park, eastern Zambia. We divided Habitat types into five groups based on physiognomic classification as: savannah wood-land, back swamp, Combretum thicket, Mopane woodland, and Terminalia-Erythrophleum. Road count method was used to sight animals across the four identified habitat types. Results showed that puku, impala, elephant, and zebra were highly selective of habitat types. Puku occurred mainly in back swamp habitat (86.6%) followed by water buck (75.0%), elephant (72.3%) and zebra (65.7%). Impala were more abundant in Terminalia-Erythrophleum woodland (69.50%) while buffalo was mainly sighted in Mopane-woodland (45.07%). Other species were more cosmopolitan. It was suggested that foraging behaviour, season and security with respect to predation were the factors singularly or collectively that influenced the observed pattern of habitat selection by large mammals.