Habitat Quality Research Articles

Biodiversity and plant-insect interactions in fragmented habitats: A systematic review

Abstract Fragmentation is threatening insect biodiversity and intricate interactions in various ecosystems. Ecological interactions – especially those involving plants and insects – are significantly impacted by fragmented habitats. Because of fragmentation, edge effects and reduced habitat connectivity and quality affect insect species diversity, abundance, behavior, movement, life cycles, and interactions with plants, e.g., pollination, herbivory, and seed distribution. To a large degree, ecosystem services or processes are mediated by these interactions. While fragmented habitats create suitable conditions for invasive alien plants (IAPs), such invasions modify native plant composition and herbivorous insect communities because they cause a decline or loss in insect biodiversity. A systematic review was conducted by reviewing eighty-eight (88) articles to gather evidence for fragmentation effects on insect biodiversity, insects’ behavior and adaptations, plant-insect interactions (i.e., pollination, herbivory, and seed dispersal), and its influence on IAP invasions. This review deduced that any change in insect community composition and diversity due to fragmentation can have cascading effects on ecosystem processes within habitats. It further contends that successful conservation and management of fragmented habitats requires an understanding of the intricate dynamics of plant-insect interactions. However, the long-term resilience and health of ecosystems can be guaranteed by supporting sustainable land use, improving connectivity, and restoring habitats. These actions may help stop and/or reduce the effects of fragmentation on insect biodiversity and support the livelihoods and well-being of millions of people.

Macrophyte Diversity and Ecosystem Services in Wetlands of Eastern Ranchi: A Multi-site Analysis of Abundance, Frequency, and Importance Value Index Patterns

Macrophytes, visible aquatic and semi-aquatic plants, are integral components of wetland ecosystems, playing crucial roles in maintaining biodiversity, providing ecosystem services, and ensuring ecological stability. This research examines the distribution, abundance, frequency, diversity, and ecosystem services of macrophytes in several wetlands (W1-W7) located in the eastern part of Ranchi district, Jharkhand, India. The study sites include Bundu Lake, Hindalco Pond, Choga Bada Talab, Raja Bandh, Kita Uparbandh, Tamar Bada Talab, and Rukka Dam. A quantitative assessment using quadrat sampling and phytosociological methods was conducted from September 2022 to March 2023 to ensure accurate data collection and analysis. The study documented 78 macrophyte species belonging to 33 families and 58 genera. Emergent macrophytes were found to be the dominant life form, constituting 69% of the recorded species. Several species demonstrated high frequency and abundance across multiple study sites, including Pontederia crassipes Mart., Alternanthera philoxeroides (Mart) Griseb., Hydrilla verticillata (L.f.) Royle, Ipomoea aquaticaForssk., and Nymphoides hydrophyllum (Lour.) kuntze. To assess the diversity and ecological characteristics of the macrophyte communities, various diversity indices were calculated. These included the Shannon-Wiener index, Simpson's index, Margalef index, and Pielou's Evenness index. The results of these analyses revealed high levels of macrophyte diversity and evenness within the studied wetlands. Twenty-one macrophytes were identified as key species and nine species as rare species based on their IVI (Importance Value Index) scores. The key species were found to provide essential ecosystem services, including erosion control, water quality improvement, nutrient cycling, carbon sequestration, and habitat provision for other organisms. This research contributes significantly to our understanding of macrophyte diversity patterns and traits-based ecosystem services in wetland ecosystems. The findings of this study have important implications for the development and implementation of effective conservation strategies for these vital wetland ecosystems in the eastern Ranchi (W1- W7).

Cougar spatiotemporal response to human activities in a multi‐use forest landscape on southern Vancouver Island

Recreational activities have been shown to induce fear responses in carnivores, causing both spatial and temporal displacement that can have cascading effects on the wider ecological community. Cougars Puma concolor are one species that are particularly sensitive to human disturbances as they have been found to be impacted by various forms of recreational activities. This research aimed to quantify the spatial and temporal responses of cougars to human activities on southern Vancouver Island in British Columbia, Canada. Fifty camera traps were deployed in a systematic probabilistic sampling design and data from the summer season of 2022 was used for analyses. We modeled cougar weekly occurrence frequency in relation to human (hikers and bikers) occurrence frequency, vehicle occurrence frequency, and landscape features, using generalized linear mixed models. Temporal responses of cougars were investigated using activity pattern analysis to compare activity patterns within a recreation area and a restricted public access reserve. Cougars avoid occurring at sites where recreationalists frequently visited, but not sites frequented by vehicles on unpaved roads. We observed minimal influence of habitat features or prey availability on cougar occurrence frequency, as measured by information loss (AICc scores). Activity pattern analysis did not demonstrate significant differences in cougar daily activity patterns between the recreation area and no‐entry reserve, suggesting a lack of temporal response. These results suggest recreational activities on southern Vancouver Island are impacting cougar spatial – but not temporal – behavioral dynamics. Insights on how cougars respond to recreational activities can help inform landscape management and policies by providing information that can be used to mitigate negative impacts and thus ensure that recreational activities are remaining compatible with conservation efforts.

Unveiling the Secrets: How Landscape Patterns Shape Habitat Quality in Northeast China Tiger and Leopard National Park

The Northeast China Tiger and Leopard National Park (NCTLNP) is a critical habitat for the endangered Amur tiger and Amur leopard, making it a global biodiversity hotspot. This study explores how changes in landscape patterns have influenced habitat quality in the park, aiming to develop strategies for enhancing biodiversity conservation and ensuring the park’s long-term sustainability. From 2012 to 2017, habitat quality in the NCTLNP experienced a significant decline; however, the launch of the national park pilot program in 2017 resulted in improvements, particularly in core protected areas, where habitat quality increased and landscape fragmentation decreased. These findings indicate that the national park initiative reduced the degradation of habitat quality. Key landscape metrics, especially the Shannon Diversity Index (SHDI), were found to significantly affect habitat quality. Additionally, the interaction between SHDI and landscape contagion (CONTAG) played a pivotal role in shaping habitat quality over time. Areas with high SHDI and low CONTAG showed declines in habitat quality, pointing to the need for focused conservation efforts. This study offers valuable insights for policymakers seeking to improve habitat quality through targeted landscape management practices.

Assessment of exurban expansion on water quality in Rock Creek of the Yellowstone River in southcentral Montana, USA

Exurban expansion can degrade significantly stream water quality. This study was conducted on Rock Creek of the Clark’s Fork of the Yellowstone River in southcentral Montana, USA. The impact from recent exurban expansion was evaluated by a baseline study measuring total suspended solids (TSS), total phosphorus (TP), total nitrogen (TN) and nitrites (NO2) and nitrates (NO3) together. This was part of a larger study by Montana Department of Environmental Quality to assess water quality, identify sources of pollution and develop total maximum daily loads (TMDL). Water samples were measured for these analytes at nine (2022) and 11 (2023) locations with different land-uses over seven periods (both years). Seasonal spikes, corresponding to runoff (2022, late May), occurred in TSS, TP and TN. Data from 2023 showed similar but reduced levels occurring in June for TSS and TP. Total suspended solids, TP, NO2 and NO3 concentrations increased by location year over year, 2022 to 2023. The high relative concentrations of NO2 and NO3 in Rock Creek are primarily associated with exurban expansion, a significant threat to stream quality and wildlife habitat in Montana. The results will assist county planners in land use decisions.

A multi-objective approach for timber harvest scheduling to include management of at-risk species and spatial configuration objectives.

Sustainable forestry typically involves integration of several economic and ecological objectives which, at times, may not be compatible with one another. Multi-objective prioritization via harvest scheduling programs can be used to elucidate these relationships and explore solutions. One such program is a spatially explicit harvest scheduler that adopts the Metropolis-Hastings algorithm to iteratively find management solutions to achieve multiple objectives (Habplan). Although this program has been used to address forest management scheduling and simulation-based tasks, its utility is constrained by time-intensive data preparation and challenges with incorporating spatial configuration objectives. To address these shortcomings, we introduce an open-source software package, HabplanR, streamlines data preparation, sets parameters, visualizes results, and assesses spatial components of ecological objectives. We developed four example objectives to incorporate into a multi-objective management problem: habitat quality indices for three species "types" (open, closed, and intermediate-canopy-associated species), and harvested pine pulpwood (revenue). We demonstrate the utility of this package to find management schedules that can accommodate potentially conflicting habitat needs of species, while achieving economic targets. We produced 100 software runs and prioritized individual objectives to select four management schedules for further comparisons. We compared outcome differences of the four schedules, including a spatial comparison of two high performing schedules. The software package makes costs and benefits of different schedules explicit and allows for consideration of the spatial configuration of management outcomes in decision-making.

Limited evidence for the influence of the physical structure and floristics of habitat on a boreal forest bird community

Studies conducted at regional and continental scales show that avian richness, community composition, and abundance are associated with variation in the vegetation physical structure and community composition (floristics) within broad habitat types. The relative contributions of physical structure and floristics are contested, and relationships are often taxon-specific. We used 110 microphone arrays deployed across 90 sampling locations and two breeding seasons to survey an avian community in the boreal forest in Labrador, Canada. Our objectives were (1) to describe the avian community of an underrepresented portion of the boreal forest, (2) to estimate the relationships between avian species richness and habitat characteristics, (3) to estimate if species detection at a given location was related to local habitat characteristics, and (4) to investigate the spatial and temporal patterns of the avian community composition. We detected 32 species at our sampling locations; physical structure and floristics were not related to avian species richness, although estimates of richness were higher on warmer days and lower on windier days. Habitat characteristics were associated with the detection of Boreal Chickadee (Poecile hudsonicus), Yellow-rumped Warbler (Setophaga coronata), American Robin (Turdus migratorius), and Black-throated Green Warbler (Setophaga virens). Finally, avian community composition was only moderately consistent among three categorical forest types and between audio sampling periods in two consecutive breeding seasons. Overall, we show that the structural and floristic traits measured at our study site are not related to the detection of most avian species or to species richness.

Impact of Climate and Vegetation Dynamics on the Ecosystem Services of Subtropical Forests—A Case Study of Baishanzu National Park Area, China

Forest ecosystems, as the primary component of terrestrial ecosystems, provide essential ecosystem services (ESs) critical for sustainable human development. However, changes in climate and vegetation can alter these forest ESs. Understanding the complex relationships between regional climate, vegetation, and ESs is key to ensuring the sustainable management of forest ESs. Therefore, this study, using Baishanzu National Park as a case example, analyzed the impacts of regional climate and vegetation dynamics (vegetation coverage, forest type, and forest structure) on forest ESs, specifically water yield (WY), soil conservation (SC), net primary productivity (NPP), and habitat quality (HQ). The results indicate that from 2000 to 2020, the forest Composite Index of Ecosystem Services (CIES) in Baishanzu National Park increased. Climate and vegetation dynamics have significant effects on forest ESs. Specifically, changes in WY and SC are primarily influenced by climate change, while changes in NPP and HQ are mainly affected by changes in forest type and structure. Complex trade-offs and synergies exist among different ESs, and the driving mechanisms of climate and vegetation changes on ES variations are also complex, involving both direct and indirect effects, with significant spatial heterogeneity. This study provides important references for the sustainable management and appropriate restoration of regional forest ESs.

Local biodiversity actions for sustainable development goal 15 (life on land) in China

AbstractAchieving sustainable development goal (SDG) 15 (life on land) depends on local conservation efforts. However, the lack of species data and inadequate ecological corridor designs significantly hinder conservation efficiency. This study focused on Sanmenxia City in China and utilized the Integrated Valuation of Ecosystem Services and Trade‐offs model, the GeoDetector model, and the Minimum Cumulative Resistance model to identify potential habitats, their influencing factors, and ecological corridors. We then adopted a new corridor feasibility model to compare the impacts of different ecological resistance, thereby identifying key biodiversity spaces. Results indicated a decline in habitat quality since 2000, primarily influenced by climate, topography, and land use. Simulation‐derived corridors using the habitat quality method were more effective than those based on expert experience. We identified 2808.45 km2 of potential habitats and 20 critical corridors for land animals. This study deepens the understanding of ecological resistance impacts on corridors and provides new insights into local actions for SDG 15.

Mapping and Identification of Ecosystem Services Hotspots in the Brazilian Pampa Biome.

The intensification and expansion of changes in land use and land cover (LULC) can reduce the availability and quality of natural habitats and ecosystem services (ES). These changes have generated environmental damage in different parts of the world, especially in biomes more susceptible to modifications, such as the Pampa biome in the extreme south of Brazil. The Pampa biome has been neglected by environmental protection laws, despite its broad ecosystem and social importance. In this study, we used InVEST models to map and quantify five ES provided (i.e., water supply, carbon stock, groundwater stock, sediment retention and habitat quality) by 14 watersheds distributed in the Brazilian Pampa biome as well as determine ES hotspots by summing the areas with high provision of ES. We identified low availability of water supply and groundwater stock in practically the entire study area. High sediment retention and carbon stock were reported in areas with the presence of native vegetation. In addition, despite the large degraded areas, we observed high habitat quality associated with native vegetation in all studied watersheds. The hotspots varied spatially in the study area according to the proposed scenarios, the supply and the overlap of ES. Scenario 1 (>50% of ES) presented a larger area of high provision of ES. In contrast, scenario 2 (>75% of ES) presented smaller patches of areas with high provision of ES distributed across different watershed. We observed that large territorial extensions with high and medium provision of ES are vulnerable to the negative effects of LULC. Our study presented scenarios that indicate areas of high provision of ES, contributing to a more practical application, being a simplified and useful tool that can assist conservation and sustainable policies.

Hymenochaete ametzii sp. nov. (Hymenochaetales, Basidiomycota), an endangered bark-dwelling species inhabiting old Quercus pyrenaica trees from the Iberian Peninsula

Bark-dwelling fungi represent a group of ecologically highly specialized organisms. This study deals with an undescribed species of Hymenochaete characterized by specifically inhabiting the bark of Pyrenean oak (Quercus pyrenaica), and producing effuse-reflexed basidiomata and mainly globose to subglobose basidiospores. Maximum Likelihood and Bayesian analyses of the nuclear ITS-LSU regions revealed that the sequences of H. ametzii form a monophyletic group with a low intraspecific variation and substantially different from closest taxa, further supporting its recognition as a species. The seven localities H. ametzii is known from are old forests with a long ecological continuity, containing large old Q. pyrenaica trees, and are located in the supramediterranean belt of the Mediterranean biogeographical region. Based on habitat availability and field counts of colonized trees, the global population of H. ametzii is estimated at 8,670 mature individuals. Considering that the traditional use of old Q. pyrenaica stands ceased several decades ago in the Iberian Peninsula, with a consequent decline in habitat quality and availability, it is concluded that H. ametzii is Endangered (EN) according to the IUCN criteria.

Restoring historical moose densities results in fewer wolves killed for woodland caribou conservation

AbstractWoodland caribou (Rangifer tarandus caribou) are declining across much of their distribution in Canada in response to habitat alteration, leading to unsustainable predation, particularly by wolves (Canis lupus). Habitat alteration can benefit the primary prey species of wolves (moose [Alces alces] and deer [Odocoileus spp.]) by creating early seral conditions that contain more of their preferred food types. This increase in primary prey populations results in elevated wolf abundance and heightened predation pressure on caribou. In response to the elevated wolf populations and the risks to caribou, managers have reduced wolf abundance in key areas. Ecological theory suggests that reducing wolf abundance would release moose from the top‐down effects of wolf predation, potentially allowing moose populations to grow. Elevated moose abundance thus has the potential to cause wolf populations to rebound quickly each year following reductions, suggesting a possible link between moose abundance and the number of wolves killed for caribou conservation. To test this idea we used a unique management situation in British Columbia and Alberta, Canada, where lethal wolf removals were annually conducted across specific southern mountain caribou population ranges and, in some places, moose populations were concurrently reduced via liberalized hunting. We used indices of moose abundance and wolf removal data to test the hypothesis that reducing moose populations to a historical abundance target by hunting leads to fewer wolves killed for caribou conservation. After controlling for habitat quality, wolves removed per km2 was 3.2 times lower in areas with reduced moose density ( = 1.55 wolves/1,000 km2 ± 0.33 [SE]) than in those without reduced moose density ( = 5.02 wolves/1,000 km2 ± 0.52). However, the average number of wolves removed per year decreased under both conditions. After 9 years, there was a 35% reduction in the predicted difference in the annual removal between areas with and without moose reduction. Our results suggest that policies that do not reduce or stabilize moose abundance will result in the removal of more wolves to increase caribou abundance. Like wolf reductions, moose reductions can also be controversial and affect local harvesters. Thus, understanding the consequences of actions that support caribou recovery is essential to supporting evidence‐based policy discussions.

Benthic habitat mapping of the glass sponge (Vazella pourtalesii), and associated community composition on Sambro Bank, Scotian Shelf, Canada

Sponges have been identified as ecosystem engineers, providing habitat structure for many other benthic organisms, but are vulnerable to the impacts of bottom contact fishing. The Sambro Bank Sponge Conservation Area (SBSCA) protects a globally unique aggregation of the glass sponge (Vazella pourtalesii) off the east coast of Canada. Here, we present the first comprehensive, high-resolution mapping study of this site. Multispectral multibeam echosounder (MBES) mapping at three operating frequencies and benthic drop camera surveys were conducted at the SBSCA in 2022. Video data were analysed to quantify the abundance and location of V. pourtalesii and all associated benthic fauna and sediment characteristics. Using the MBES data as predictor variables, the study created a generalized linear mixed model of V. pourtalesii presence in the SBSCA, with 90.1% accuracy. Four seafloor benthoscape classes were identified from the video imagery, with statistically distinct benthic macrofaunal communities associated with each class. The study creates a baseline assessment of the current community composition and habitat characteristics of the site, which can be used for future ecological monitoring and management.

Nature-Based Solution for Climate Change Adaptation: Coastal Habitats Restoration in Xiamen Bay, China

Nature-based solutions (NbSs) of biodiversity conservation and ecosystem restoration have been paid increasing attention as an essential approach to slow down climate change. However, to what degree an NbS approach will contribute to the combined effects of human intervention and climate change has not been well studied. From a habitat quality perspective, we set four NbS scenarios to analyze whether the NbS—mangrove restoration in particular—will be enough for climate change in Xiamen Bay of Fujian Province, China. The habitat quality module of the InVEST model (InVEST-HQ) and the Sea Level Affecting Marshes Model (SLAMM) were used to simulate the spatial-temporal changes in habitat types and habitat quality. Results show that (1) rising sea levels will cause coastal squeeze effects, impacting habitat quality due to erosion and inundation in the study area; (2) mangrove restoration is an effective way to mitigate climate change effects and to increase habitat quality; and (3) further analysis of the effectiveness of mangrove restoration shows that the consideration of mangrove fragmentation effects and sea-use impacts are necessary. The findings in this study will enrich the international discussion of NbSs to climate change in coastal areas.

How to Achieve the Ecological Sustainability Goal of Ecologically Fragile Areas on the Qinghai-Tibet Plateau: A Multi-Scenario Simulation of Lanzhou-Xining Urban Agglomerations

The future of the ecologically fragile areas on the Qinghai-Tibet Plateau (QTP) is a matter of concern. With the implementation of the Western Development Strategy, the Lanzhou-Xining Urban Agglomeration (LXUA) has encountered conflicts and compromises between urban expansion, ecological protection, and farmland protection policies in the rapid development of the past 2 decades. These deeply affect the land use layout, making the ecological sustainable development of the ecologically fragile areas of the QTP a complex and urgent issue. Exploring the impact of different policy-led land use patterns on regional ecosystem services is of great significance for the sustainable development of ecologically fragile areas and the formulation of relevant policies. Following the logical main line of “history-present-future”, the Patch-level Land Use Simulation (PLUS) model, which explores potential factors of historical land use, and the Integrated Valuation of Ecosystem Services and Trade-offs (InVEST) model were used to construct three future scenarios for the modernization stage in 2031 dominated by different land use policies in this study. These scenarios include the Business-as-Usual Scenario (BS), the Cropland Protection Scenario (CP), and the Ecological Protection Scenario (EP). The study analyzed and predicted land use changes in the LXUA from 2001 to 2031 and assessed carbon storage, habitat quality at different time points, and water yield in 2021. The results indicated that land use changes from 2001 to 2021 reflect the impacts and conflicts among the Western Development Strategy, ecological protection policies, and cropland preservation policies. In 2031, construction land continues to increase under all three scenarios, expanding northwards around Lanzhou, consistent with the actual “northward expansion” trend of Lanzhou City. Changes in other land uses are in line with the directions guided by land use policy. By 2031, carbon storage and habitat quality decline under all scenarios, with the highest values observed in the EP scenario, the lowest carbon storage in the BS scenario, and the lowest habitat quality in the CP scenario. Regarding water yield, the LXUA primarily relies on alpine snowmelt, with construction land overlapping high evapotranspiration areas. Based on the assessment of ecosystem services, urban expansion, delineation of ecological red lines, and improvement of cropland quality in the LXUA were proposed. These findings and recommendations can provide a scientific basis for policy makers and planning managers in the future.

Assessment and Multiscenario Simulation of Land Use and Ecosystem Services Interactions in Inner Mongolia

ABSTRACTBalancing land development and ecological protection poses significant challenges for sustainable development in arid and semi‐arid regions like Inner Mongolia. This study uses the integrated valuation of ecosystem services (ESs) trade‐offs model and patch‐generated land‐use simulation model to analyze the impacts of land‐use/cover (LULC) changes on ESs from 2005 to 2020 and simulate scenarios for 2035. The results show that (1) ecological governance efforts improved the comprehensive ecological index (CEI), habitat quality, and soil conservation capacity but led to a decrease in the annual water yield (AWY) in the eastern region. Additionally, the conversion of grassland and forest land to construction land and farmland caused slight declines in carbon sequestration and increased nitrogen export. (2) The external driving factors of different ESs vary, and the internal relationships within ESs have also changed. Notably, changes in LULC transformed the synergy between AWY and habitat quality into a trade‐off relationship after 2010 due to increased forest and grassland. (3) Among the five land‐use scenarios, the comprehensive development scenario combined with zonal management strategies can achieve the sustainable development goals of economic growth, ecological protection, and food security. This study clarifies the internal and external factors affecting ESs in Inner Mongolia and provides a scientific basis for future LULC management and ecological governance policies. It fills a gap in the spatiotemporal simulation of ESs under multiple scenarios in this region. These findings offer valuable references for promoting sustainable development in similar arid and semi‐arid regions, highlighting the importance of integrating multi‐objective strategies and adaptive management practices to balance ecological and economic objectives.

Characteristics, impacts and future research directions of Mongolian peatlands

Mongolia is an important peatland distribution area in the world. Over the past few decades, Mongolian peatlands have undergone significant degradation due to climate change and human activities, yet there remains substantial uncertainty about the impacts of Mongolian peatland changes on regional environment and human society. Here, compiling the data of peatland distribution, climate, human activity, as well as the permafrost, we systematically review the distribution, changes, and the ecosystem service values of the Mongolian peatlands in the face of climate warming and intensifying human activities. The current data show that the total area of peatland in the Mongolian is 15500~27000 km2, and most of the peatlands are distributed in permafrost regions, while the accuracies of these maps were not assessed. In addition to climate warming, overgrazing, mining activities, and transportation, we suggest permafrost degradation also poses significant threatens on the peatlands. Although the importance of Mongolian peatlands has been recognized, the ecosystem service values, including water provision, habitat quality, carbon fixation, soil conservation, and wind erosion prevention largely remain unknown. Currently, efforts have been made to protect the Mongolian grassland, but there are no specific measures to combat peatland degradation. To better understand the changes and roles of peatland ecosystems in regulating regional development, we propose three research directions for future studies: 1) produce a detailed map of peatland distribution based on field survey data, new remote sensing data, and new algorithms; 2) Unveil the mechanisms underlying the interaction of peat, vegetation, and permafrost; 3) Evaluate the ecosystem services of Mongolian peatlands. These knowledges are the scientific foundation to propose and implement measures to protect, maintain, and sustainably utilize peatlands in Mongolia.

Spatial and temporal partitioning between eastern gray and fox squirrels in a Central Hardwood forest

Abstract Congeneric fox squirrels (Sciurus niger) and eastern gray squirrels (S. carolinensis) compete for resources within North American temperate forests. Both species exhibit regional variation in morphology and behavior—potentially due to differences in geography, community composition, or ecological pressures between forested regions. While many have studied these species in other forested regions of the United States, recent assessments of partitioning between these species in Central Hardwood forests remain scarce. We investigated spatial and temporal partitioning between squirrel species using photographic captures from camera traps at 2 scales (i.e., camera location and camera cluster) across a 16,058-km2 region of southern Illinois, United States, during January to April 2008 to 2010. We fitted single-season single-species and co-occurrence occupancy models to assess spatial partitioning at both scales and used kernel density analysis to assess temporal partitioning. We recorded 3,044 photographic captures of focal species (n = 918 fox squirrels and 2,126 eastern gray squirrels). Fox Squirrel occupancy was 0.26 ± 0.09 (SE) and 0.50 ± 0.17 at the camera location and camera cluster scales, respectively. Eastern Gray Squirrel occupancy was 0.47 ± 0.07 and 0.84 ± 0.23 at the camera location and camera cluster scales, respectively. Fox Squirrel occupancy increased with further distances to roads and had scale-dependent relationships to forest structure. Eastern Gray Squirrel occupancy increased with more hardwood basal area. Co-occurrence was influenced by distance to road at the camera location scale. We found a moderate level of activity overlap between species (Δ = 0.63, CI = 0.60 to 0.67); however, no evidence of temporal partitioning was observed. Habitat characteristics and spatial scale appear more influential in partitioning eastern gray and fox squirrels in Central Hardwood forests than peak activity.

Дополнение к лихенофлоре России IV. Bactrospora patellarioides и заметки по российским видам рода Bactrospora (Arthoniales, Ascomycota)

During the study of the lichen flora of the Utrish Nature Reserve (Abrau Peninsula, Krasnodar Territory), a Mediterranean-Atlantic lichen species Bactrospora patellarioides was identified for the first time for Russia and the Caucasus based on morphological and anatomical characteristics. This species grows on the trunk of Juniperus excelsa in the sub-Mediterranean xerothermic undisturbed pistacia-juniper community on the Black Sea coast. The species was previously known from the Mediterranean regions of Europe, Western Asia, North Africa, and is also known from Macaronesia and Western North America. Detailed description, habitat characteristics, ecology, and general distribution of species are presented. Notes on the genus Bactrospora are given, and a key to the Russian species (B. brodoi, B. dryina and B. patellarioides) is provided.

A spatial matrix factorization method to characterize ecological assemblages as a mixture of unobserved sources: An application to fish eDNA surveys

Abstract Understanding how ecological assemblages vary in space and time is essential for advancing our knowledge of biodiversity dynamics and ecosystem functioning. Metabarcoding of environmental DNA (eDNA) is an efficient method for documenting biodiversity changes in both marine and terrestrial ecosystems. However, current methods fail to detect and display the biodiversity structure within and between eDNA samples limiting ecological and biogeographical interpretations. We present a spatial matrix factorization method that identifies optimal eDNA sample assemblages—called pools—assuming that taxonomic unit composition is based on a fixed number of unknown sources. These sources, in turn, represent taxonomic units sharing similar habitat properties or characteristics. The method aims to reduce the multi‐taxa composition structure into a low number of dimensions defined by these sources. This method is inspired by admixture analysis in population genetics. Using a marine fish eDNA survey on 263 sampling stations detecting 2888 molecular operational taxonomic units (MOTUs), we apply this method to analyse the biogeography and mixing patterns of fish assemblages at regional and large scales. At large scale, our analysis reveals six primary pools of fish samples characterized by distinct biogeographic patterns, with some mixtures between these pools. We identify pools composed of unique sources, corresponding to distinct and more isolated regions such as the Mediterranean and Scotia Seas. We also identify pools composed of a greater mix of sources, corresponding to geographically connected areas, such as tropical regions. Additionally, we identify the taxa underpinning the formation of each pool. In the regional analysis of Mediterranean eDNA samples, our method successfully identifies different pools, allowing the detection of not only geographic gradients but also human‐induced gradients corresponding to protection levels. Spatial matrix factorization adds a new method in community ecology, where each sample is considered as a mixture of K unobserved sources, to assess the dissimilarity of ecological assemblages revealing environmental and human‐induced gradients. Beyond the study of fish eDNA samples, this method has the potential to shed new light on any biodiversity survey and provide new bioindicators of global change.