You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II1 Apr 2017MP19-09 DAILY HYDROGEN SULFIDE THERAPY DURING PROLONGED URETERIC OBSTRUCTION ENABLES EARLY RENAL RECOVERY FOLLOWING DECOMPRESSION Shouzhe Lin, Dameng Lian, Weihua Liu, Aaron Haig, Ian Lobb, Ahmed Hijazi, Matthew Whiteman, and Alp Sener Shouzhe LinShouzhe Lin More articles by this author , Dameng LianDameng Lian More articles by this author , Weihua LiuWeihua Liu More articles by this author , Aaron HaigAaron Haig More articles by this author , Ian LobbIan Lobb More articles by this author , Ahmed HijaziAhmed Hijazi More articles by this author , Matthew WhitemanMatthew Whiteman More articles by this author , and Alp SenerAlp Sener More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.3251AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Renal injury acquired during prolonged ureteral obstruction (UO) can lead to permanent renal dysfunction. Treatments aimed at mitigating renal injury during UO are limited. Hydrogen sulfide (H2S), an endogenous gasotransmitter, has been shown to ameliorate tissue injury. We have previously demonstrated that daily H2S treatment can reduce the histopathological markers of renal injury following 30 days of unilateral ureteral obstruction (UUO). The current study employs UUO followed by reimplantation to investigate the effects of daily H2S on renal function following the relief of obstruction. METHODS The left ureter of male Lewis rats were ligated at the ureterovesical junction. Starting on post-operative day (POD) 1 and every day for 13 days, rats received either daily intraperitoneal (IP) injection of phosphate buffered saline (PBS) vehicle or 200µmol/kg of GYY4137 (slow-releasing H2S donor) in PBS. On POD 14, the ligature was removed and the left ureter was reimplanted into the bladder, and a right nephrectomy performed so that the rat is solely dependent on the left kidney. Urine and serum samples were collected to monitor renal function. On POD 30, the left kidney was removed and tissue sections were stained with hematoxylin and eosin (H&E) to measure cortical thickness. RESULTS 67% of control rats, compared to 100% of H2S-treated rats, lived until POD 30. Histological analysis showed a significant decrease in cortical thickness (P<0.0001) in control rats when compared to Sham rats, which was significantly rescued upon treatment with H2S (P<0.0001). Serum creatinine (SCr) in control rats, but not H2S-treated rats, was significantly increased when compared to Sham animals on POD 3 and 7 (P<0.05). Following reimplantation (PODs 17, 21, 24 and 30), SCr in both control and treatment groups were significantly elevated when compared to Sham. Interestingly, H2S treatment drastically decreased SCr levels when compared to control animals on all PODs after reimplantation. Proteinuria continued to be higher in both control and treatment groups following reimplantation compared to Sham (P<0.05), however, H2S treatment group showed markedly reduced proteinuria compared to the control group. CONCLUSIONS We show, for the first time, that daily H2S therapy rescues renal function and preserves renal architecture following UO. H2S may one day become a clinically viable pre-emptive therapy against renal injury associated with UO and contribute to improved renal function in patients with UO. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e232-e233 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Shouzhe Lin More articles by this author Dameng Lian More articles by this author Weihua Liu More articles by this author Aaron Haig More articles by this author Ian Lobb More articles by this author Ahmed Hijazi More articles by this author Matthew Whiteman More articles by this author Alp Sener More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...