Background: The implementation of routine fixed-time artificial insemination programs can generate significant waste and costs, which good management practices can help mitigate. The objective of the study was to assess the effects of second- and third-use progesterone devices on estrus characteristics and pregnancy rates in synchronized crossbred Gyr cows. Methods: Ten cows were synchronized with first-use Bovine Intravaginal Progesterone Devices (DIB), nine with second-use DIB and ten with third-use DIB. All cows were ³90 days postpartum, had an average milk production of 6.9±1.9 L/day and were aged 4-10 years. Estrus signs were recorded at insemination and pregnancies were confirmed at 60 days by ultrasonography. Result: No significant associations were found between DIB use intensity and estrus detection patch response, mucus color, body condition score (CC), calvings, or breed purity (p greater than 0.05). Body condition was associated with uterine turgor tone (p less than 0.05) and 100% of cows with CC 3.0 showed turgor tone 3. The highest pregnancy rate was found in second-use DIB (55.6%) and CC 2.8 (47.1%), though neither association was significant (p greater than 0.05). DIBs may be used up to a second-use, as cervical mucosal stimulation and pregnancy rate remain unaffected.
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