Gymnema Sylvestre Leaf Extract Research Articles

Effect of <i>Gymnema sylvestre</i> Extract on the Regulation of AMPK-GLUT4 Mediated Signaling Pathway on Insulin Resistance in a PCOS Rat Model

This study was intended to assess the effect of Gymnema sylvestre leaf extract on the AMPK-GLUT4 mediated signalling pathway on insulin resistance in polycystic ovarian syndrome-induced rats. For the induction of PCOS in rats, 1 mg/kg of letrozole was given orally for 21 days, after that metformin (300 mg/kg), Gymnema sylvestre leaf extract (200 mg/kg, and 400 mg/kg p. o.) were administered for further 28 days. Letrozole-induced PCOS rats illustrated significant estrus irregularity, and sex hormonal abnormality, and developed insulin resistance as indicated by increased fasting glucose levels, an increased rate of glucose clearance, and also decreases in GLUT4 and AMPK mRNA expression in ovarian cells. Ovarian histology in PCOS rats also showed many follicular cysts, atretic follicles, and the absence of the corpus luteum. These changes were significantly reversed by the treatment of G. sylvestre in a dose-dependent manner. It might be mediated through its insulin resistance modulating property by the activation of AMPK & GLUT4 expression on ovarian endometrial cells.

Cytotoxicity Study of Gold Nanoparticle Synthesis Using Aloe vera, Honey, and Gymnema sylvestre Leaf Extract.

Gold nanoparticles (AuNPs) have gained importance in the field of biomedical research and diagnostics due to their unique physicochemical properties. This study aimed to synthesize AuNPs using Aloe vera extract, honey, and Gymnema sylvestre leaf extract. Physicochemical parameters for the optimal synthesis of AuNPs were determined using 0.5, 1, 2, and 3 mM of gold salt at varying temperatures from 20 to 50 °C. X-ray diffraction was used to evaluate the crystal structure of AuNPs, which came out to be a face-centered cubic structure. Scanning electron microscopy and energy-dispersive X-ray spectroscopy analysis confirmed the size and shape of AuNPs between 20 and 50 nm from the Aloe vera, honey, and Gymnema sylvestre, as well as large-sized nanocubes in the case of honey, with 21-34 wt % of gold content. Furthermore, Fourier transform infrared spectroscopy confirmed the presence of a broadband of amine (N-H) and alcohol groups (O-H) on the surface of the synthesized AuNPs that prevents them from agglomeration and provides stability. Broad and weak bands of aliphatic ether (C-O), alkane (C-H), and other functional groups were also found on these AuNPs. DPPH antioxidant activity assay showed a high free radical scavenging potential. The most suited source was selected for further conjugation with three anticancer drugs including 4-hydroxy Tamoxifen, HIF1 alpha inhibitor, and the soluble Guanylyl Cyclase Inhibitor 1 H-[1,2,4] oxadiazolo [4,3-alpha]quinoxalin-1-one (ODQ). Evidence of the pegylated drug conjugation with AuNPs was reinforced by ultraviolet/visible spectroscopy. These drug-conjugated nanoparticles were further checked on MCF7 and MDA-MB-231 cells for their cytotoxicity. These AuNP-conjugated drugs can be a good candidate for breast cancer treatment that will lead toward safe, economical, biocompatible, and targeted drug delivery systems.

Evaluation of Gymnema sylvestre R. Br. against Letrozole Induced Polycystic Ovarian Syndrome in rats

Objectives: To evaluate the therapeutic potential of Gymnema sylvestre (GS) leaf ethanolic extract against letrozole induced polycystic ovarian syndrome (PCOS) in rats. Methods: Thirty female virgin rats (180 - 250g) with regular estrus cycles were used and divided into five groups of six animals each. Group-I serves as normal control receives 1ml of 0.5% carboxyl methyl cellulose orally; group-II to V were treated with letrozole (1mg/kg/b.w. p.o.) for 21 days to induce PCOS. Induction of PCOS was confirmed by measuring menstrual irregularity. After induction of PCOS, group-II serves as PCOS control, group-III receives metformin (20 mg/kg/b.w. p.o.) as standard, and group- IV to V treated with G. sylvestre ethanolic extract 200mg/kg/b.w. and 400mg/kg/b.w. p.o. respectively for 28 days. After completion of the treatment schedule oral glucose tolerance test was carried out, followed by serum lipid profile (LDL, VLDL, HDL, and TG), and hormonal analysis was carried out. Then the animals have sacrificed and the ovaries were then processed for histological examination. Results: The results of this study exhibited irregularity in the estrus cycle, a significant increase in serum blood glucose, and an altered serum lipid profile in PCOS control. In addition, the PCOS control rats showed a significant increase in serum luteinizing hormone, testosterone, and a decrease in estrogen, follicle-stimulating hormone, and progesterone. These changes were significantly reversed by G. sylvestre in a dose dependent manner. Histopathology of the ovary also showed an almost normal ovary in the treatment groups. Conclusion: In conclusion, the treatment of ethanolic leaf extract of Gymnema sylvestre reduces insulin resistance by improving glucose utilization, regularize menstrual irregularity and promote ovulation by correcting the hormonal irregularities and improve the development of follicles in PCOS condition.

Green facile synthesis of Ag-doped ZnO nanoparticles from Gymnema sylvestre leaf extract and investigation of their Antibacterial activity

The unique characteristics of the green synthesis pathway have opened the way for a new field of scientific investigation. The current study used green chemistry methodologies to produce pure zinc oxide (ZnO) (G1) and silver doped zinc oxide (Ag doped ZnO) (G2) nanoparticles (NPs) using Gymnema sylvestre (G. sylvestre) leaf extract as a reducing as well as capping agent. XRD, FESEM, EDAX, and FT-IR investigations were used to investigate the structural characterization of G1 and G2 NPs. The UV–Vis and PL analyses were used to examine the optical characteristics. The results clearly reveal that the leaf extract is the best possible stabilizing agent for the manufacture of G1 and G2 NPs, and that the generated nanoparticles operate as potent microbial agents, inhibiting the growth of a variety of dangerous microbes. Due to their eco–friendly and non–toxic compatibility, the prepared G1 and G2 NPs can be employed for therapeutic and other purposes.

Anti-Diabetic Potential of Gymnema Sylvestre: In Vitro and in Silico Analysis

Gymnema sylvestre (GS) is a powerful antidiabetic plant that has been utilized in ayurvedic, folk and homeopathic medicine for centuries. In this research, we evaluated the antidiabetic potential of methanolic leaf extract of Gymnema sylvestre. Fractionation was carried out using column chromatography and a total of twenty-eight (28) sub-fractions were obtained which were further screened and pooled into three (3) fractions (A, B and C) by thin layer chromatography based on their retention factor (Rf) values. The fractions were subjected to in vitro α-amylase and α-glucosidase inhibition activity. Some of the compounds identified from LC-MS were subjected to in silico analysis between all the ligands and the receptors with the aid of a docking software. Ligands were imported for visual screening into PyRx software while Biovia Discovery Studio Visualizer was used for protein preparation. Analysis of the properties of drug likeliness of the ligands was done via SwissADME online server according to Lipinski’s Rule of Five. Final docking analysis was done through AutoDockVina and Biovia Discovery Studio client 2020. Fraction C showed the best IC50 of 0.84µg/ml α-glucosidase inhibitory activity when compared with fraction A and B, 2.00µg/ml and 1.58µg/ml (α-glucosidase), fraction A produced the best α-amylase activity among the fractions with IC50 of 16.78µg/ml, fraction B with 23.17µg/ml and fraction C with 28.22µg/ml. Molecular docking analysis of the ligands Orcinol (-5.5 kcal/mol) showed strong binding interaction with α-amylase, followed by 3-hydroxy-3'-methoxyflavone and Curcumin (-7.1 kcal/mol and -7.6 kcal/mol respectively) compared to acarbose (-8.0 kcal/mol) and Glyinflanin A (-8.4 kcal/mol) interactions. The binding affinity of Orcinol, 3-hydroxy-3'-methoxyflavone, Curcumin and Glyinflanin A (-5.7 kcal/mol, -8.0 kcal/mol, -7.6 kcal/mol and -9.1 kcal/mol respectively) were lower compared to acarbose (-9.7 kcal/mol) interaction with α-glucosidase. Thus, compounds identified from Gymnema sylvestre were found to have antidiabetic potentials with Orcinol displaying the most effective binding affinity in potential for drug development.

Protective effect of Gymnema sylvestre leaf extract against uranium toxicity in human peripheral blood mononuclear cells

BackgroundUranium is a toxic heavy metal, naturally present in soil and water, that causes several health effects due to free radical generation and damage to DNA. Gynmenma sylvestre is a pharmacologically important medicinal plant that has been used in the treatment of diabetes and several other ailments. Accordingly, in this analysis, we intended to examine the protective effect of G. sylvestre leaf extract (GSE) against uranium toxicity. MethodsAqueous leaf extract of Gymnema sylvestre was prepared and its qualitative and quantitative phytochemical analysis was carried out. The antioxidant potential of the extract was analysed. Human lymphocytes isolated from freshly collected blood samples were treated with uranyl nitrate and GSE independently and in combination. The toxic effects of uranium and the protective effect of the leaf extract have been analyzed by MTT assay, micronucleus and γH2AX DNA damage response detection. ResultsPhytochemical analysis revealed the presence of major and important phytochemical groups such as alkaloids, flavonoids, saponins, tannins, etc. 15 μg/mL of GSE significantly reduced toxicity in the 3 different uranium concentrations (0.25, 0.50 & 0.75 mM). Cell viability percentage increased from 90.44 to 94%, 80.40 to 86% and 72.03 to 77.40% in the GSE and uranium co-exposure groups. GSE treatment led to statistically significant reduction in the average number of micronuclei observed per 1000 binucleate cells in all three groups with values 30.66, 40 and 48.33 micronuclei respectively. GSE was successful in reducing the γH2AX percentages in the treated cell groups with statistically significant protection with values 22.11 ± 1.56, 48.43 ± 0.83 and 51.14 ± 1.78 respectively. ConclusionThis study is the first to report the in vitro toxic effects of uranium in human peripheral blood mononuclear cells as well as the protective effect of Gymnema sylvestre against uranium induced damages. GSE could alleviate DNA damage in cells treated with uranium in a dose-dependent way. Together these findings emphasize that GSE could be a promising protective agent against uranium-induced damages and should be further evaluated for the identification of active principle(s).

Formulation of Silver Nanoparticles using Gymnema sylvestre Leaf Extract and In-vitro Anti-diabetic Activity

Aim: Formulation of Silver Nanoparticles Using Gymnema sylvestre Leaf Extract and In-Vitro Anti-diabetic Activity.
 Place and Duration of the Study: The present work has been carried out between April-2021 to July-2021 and at Mandasur University Mandsaur, Madhya Pradesh-458001.
 Methodology: A versatile technique was implemented for the synthesis of silver nanoparticles using aqueous and alcoholic extract of leaves of Gymnema sylvestre. The Ag-NP was subjected to UV, FTIR, XRD, and particle size analysis. The silver nanoparticles Gymnema sylvestre evaluated its anti-diabetic activity by inhibiting the enzymes α-amylase.
 Results: The reduction of silver nitrate to silver nanoparticles was confirmed by UV-Vis spectrophotometer showing a typical resonance (SPR) at about 400 nm which is specific for Ag-NPs. In FT-IR analysis the strong band peak at 3355 cm-1 in the extract is vibration bands, which may be due to the overlapping of amine N-H stretching bands. SEM and EDX images reveal that the particles are spherical and the relatively uniform shape of the silver nanoparticles was confirmed in the range of 70-100 nm in extract, whereas DLS showed good zeta potential and less particle size.
 Conclusions: The silver nanoparticles that were biologically synthesized from Gymnema sylvestre were economical, non-toxic, and environmentally benign. Due to the reducing and capping nature of the bioactive Phyto-compounds, present in the aqueous extracts. This extract is found to be suitable for the green synthesis of silver nanoparticles. Synthesized silver nanoparticles exhibit the highest level of anti-diabetic activity by inhibiting carbohydrate metabolizing enzymes such as α-amylase. Therefore, synthesized nanoparticles can be a good therapeutic agent for controlling diabetes by inhibiting enzymes that hydrolyze carbohydrates.

Effect of gamma sterilization on Gymnema sylvestre leaf extract fused Polycaprolactone nanofiber for effective wound dressing applications

Nanomaterials have been proven to speed up wound healing compared to other wound healing materials. In the present study, Gymnema sylvestre leaf extract was incorporated into polycaprolactone (PCL) nanofiber, gamma sterilized and used for efficient wound dressing applications. The synthesized nanofibers (NFs) were beadless, hydrophilic, and continuous, exhibited surface mediated antibacterial activity against wound pathogens and enhanced proliferation of fibroblast cells. The fabricated NFs can be constructively used for wound care application.

Evaluation of antimicrobial activity of leaf extract of Gymnema sylvestre

The present study was designed with the main aim to evaluate antimicrobial activity of aqueous leaf extract of Gymnema sylvestre against pathogenic microorganisms. Leaves of G. sylvestre was subjected to successive solvent extraction by continuous hot extraction (Soxhlet) with water. The extracts were diluted in dimethyl sulfoxide (DMSO) before testing the antimicrobial activity. The antimicrobial activity of extract for B. subtilis, K. aerogenus, S. aureus, E. coli, A. niger and P. chrysogenium was determined by agar well diffusion technique. The results of the present study revealed that aq. leaf extract of G. Sylvestre exhibited zones of inhibition at 100 mg/ml concentration. Aq. leaf extract of G. sylvestre exhibited zone of inhibition zone bacterial species like B. subtilis (10.55 mm), K. aerogenus (11.00 mm), S. aureus (8.50 mm), E. coli (12.50 mm), and against fungal species like A. niger (10.00 mm). In conclusion, present preliminary study delineated that the aqueous extract of G. sylvestre possesses considerable antimicrobial activities against B. subtilis, S. aureus, E. coli, K. aerogenes and A. niger. Hence, further experiments like isolation and purification of phytoactives of G. sylvestre responsible for antimicrobial activities could be recommended to carry out to further enhance the antimicrobial activities of phytoactives present in aq. leaf extract of G. sylvestre.

Green mediated synthesis of palladium nanoparticles using aqueous leaf extract of Gymnema sylvestre for catalytic reduction of Cr (VI)

Palladium nanoparticles (PdNPs) were synthesised using a microwave technique using Gymnema sylvestre (GSE) leaf extract. PdNPs were tailored to different PdCl2 and plant extract concentrations. Using XRD the crystalline existence of GSE-PdNPs has been established, confirming the formation of face-centered cubic structure. UV–Vis spectra of PdNPs confirmed the reduction of Pd2+ to Pd0. The FTIR spectra indicate the existence of functional groups which are responsible for nanoparticles reduction and progression. The SEM discloses quasi-sphere nanoparticles without agglomeration or aggregation which TEM confirms further. The reduction of Cr (VI) was performed with PdNPs (10 mg/L) and achieved a reduction of 96% within 30 min. In order to investigate the efficient reduction of chromium from Cr (VI) to Cr (III), different reaction system parameters have been optimised.

Ethanolic Leaf Extract of Gymnema sylvestre Ameliorates Hyperglycemia and Pancreatic Oxidative Stress in Alloxan induced Diabetic Rats

Diabetes mellitus (DM) is a serious metabolic disorder with altered carbohydrate, fat, and protein metabolism. In the last four decades, India has emerged as an epicenter of the global diabetes mellitus pandemic. Rapid changes in the developmental scenario, demographic changes, and living style in the Indian subcontinent have led to the explosive increase in diabetes. Present research probes with ethanolic extract of Gymnema sylvestre (500 mg/kg.b.w) for treatment of hyperglycemia and related oxidative stress caused by Alloxan (100 mg/kg.b.wt), as a diabetogenic agent. 25 rats were included in the research divided into 5 groups, each containing 5 rats. Group 1 (normal rats), Group II (Diabetic rats (DM), Group III (DM+ treated for 10 days), Group IV (DM+20 days treated), Group V (DM+30 days treated). Blood samples and pancreatic tissues were collected at each interval of time. The blood sample was used for biochemical parameter and tissues were used for the anti-oxidant assay. Gymnema sylvestre extract (GSE) showed glucose-lowering property meanwhile, insulin secretion also increased as compared to Diabetic rats. Other tests like amylase, lipase, ALT, and AST also showed significant recovery after the extract administration. Oxidative stress was found in the Diabetic group, but after extract treatment concentration of superoxide dismutase, Glutathione-S-transferase, catalase, Glutathione peroxidase, Glutathione, and Total thiol was regained. Imbalance in serum electrolyte recovered and dysregulated hematological parameters due to stress and hyperglycemia showed convincing results. The finding suggests Gymnema sylvestre could be used as a hypoglycemic as well as an anti-oxidant agent in diabetes.

Effect of Gymnema sylvestre leaf extract on Streptozotocin induced diabetic rats

Use of traditional plant as remedies to treat Diabetes mellitus plays a major role in the inexpensive alternative medicine with fewer or no side effects. Gymnema sylvestre is an herbal plant used by Indian natives for several years to treat Diabetes mellitus. The aim of the present study was to evaluate the anti-diabetic activity of Gymnema sylvestre leaf extract. The anti-diabetic activity was studied using Streptozotocin induced diabetic wistar albino rats. The preliminary phytochemical analysis of the extract of Gymnema sylvestre shows the presence of phytoconstituents such as alkaloids, saponins, phenolic compound, tannins, flavonoids, terpenoids, cardiac glycosides and absence of carbohydrates, steroids. Methanolic leaf extract did not produce mortality or morbidity while acute oral toxicity studies in wistar albino rats at 500mg/Kg body weight. The anti-diabetic activity of Gymnema sylvestre leaf extract was confirmed by oral administration of the extract to a Streptozotocin induced diabetic rat.

A green route to phytofabricated synthesis of Gymnema sylvestre conjugated silver nanoparticles (GS-AgNPs) and its antimicrobial activity

The plant-derived chemicals present in the leaf extract of Gymnema sylvestre (LEGS) commonly known as "Gurmer" has been utilized for the synthesis of Gymnema sylvestre conjugated silver nanoparticles (GS-AgNPs) at room temperature without any auxiliary reducing and capping agents. Spherical shaped GS-AgNPs of 26.6 nm average diameters were observed. A selective dose dependent antimicrobial activity of the freshly synthesized GS-AgNPs on both gram-positive Staphylococus aureus and gramnegative Proteus vulgaris strain showed the same minimum inhibitory concentration (MIC) value at 50 µg/mL, as revealed by in-vitro MIC assay. Zone inhibition study carried out by Disk agar diffusion (DAD) test showed that the GS-AgNPs is sensitive on both the bacteria cell having a comparatively better antimicrobial activity towards Gram-negative over Gram-positive bacteria. The synthesized GS-AgNPs showed very low toxic effect in normal human cells. Dichlorodihydrofluorescein diacetate (DCDHFDA) and ethidium bromide-acridine orange (EB-AO) staining studies revealed that the GS-AgNPs mainly produces Reactive Oxygen Species (ROS) by altering its electron transport chain (ETC) system and it leads to cell death or necrosis through apoptosis of bacterial cells.

Preparation of herbal curd with Gymnema sylvestre and its characterization for the treatment of liver cancer

AbstractHerbal curd was prepared by adding the Gymnema sylvestre leaf extract during fermentation and its activity against liver cancer was investigated on HepG2 cell lines by MTT (3‐[4,5‐dimethylthiazole‐2yl]‐2,5‐diphenyltetrazolium bromide) assay. The prepared herbal curd was characterized with the help of qualitative phytochemical analysis, Fourier transform infrared (FT‐IR) spectroscopy and gas chromatography‐ mass spectrometry (GC–MS). The sensory attributes and stability of the herbal curd were also determined. The qualitative phytochemical analysis of the herbal curd showed the transfer of phenolic compounds from the plant extract to herbal curd. The FT‐IR analysis exhibited that the Anhydride group present in the plant extract was infused into the herbal curd in addition to its other functional groups such as Alcohol, Alkyne, Alkene, Amide, Sulfate, Ether and Esters. The GC–MS analysis revealed the presence of 10 new compounds which were not present in curd or plant extract. The results of MTT assay showed that the herbal curd had significantly reduced the growth of HepG2 cells. The sensory evaluation proved that the herbal curd was delicious than the plant extract. Hence, it can be used as a potential food supplement for the treatment of liver cancer.Practical ApplicationsFermented milk products have been used for the prevention and treatment of various diseases including cancer and diabetes. Recently, the usage of medicinal plants for the treatment of cancer and other diseases has gained incredible attention due to their higher activity and minimal side effects. Gymnema sylvestre is a pharmacologically significant medicinal plant, used in different kinds of poly‐herbal formulations for the treatment of various diseases. In the present study, it was used for the preparation of herbal curd that will serve as an alternative remedial measure for treating liver cancer.

Green synthesis of silver nanoparticles using Gymnema sylvestre leaf extract and evaluation of its antibacterial activity

In this work, an eco-friendly and cost effective green synthesis has been employed to the fabrication of silver nanoparticles (Ag NPs) from the non-toxic aqueous Gymnema sylvestre leaf extract as a reducing agent. The surface plasmon resonance band at 442 nm in the UV–visible absorption spectra strongly affirms the development of Ag NPs in the aqueous medium. X-ray diffraction spectra confirm that the synthesized samples have face centre cubic structure of Ag NPs and the EDX analysis also supports the formation of Ag metal NPs by the energy peak at 3 keV. TEM study discloses that most of the synthesized Ag NPs are spherical in shape and the average particle size falls in the ranging between 20 and 30 nm. Gymnema sylvestre plant extracts biomolecules involved in the reduction process was determined by FT-IR analysis. The synthesized Ag NPs showed good antibacterial activity on the growth of both Staphylococcus aureus and Escherichia coli.

Anti-cancer effect of Gymnema sylvestre Leaf Extract against MG63, Human Osteosarcoma cell line - An in vitro analysis

Anti-cancer effect of Gymnema sylvestre Leaf Extract against MG63, Human Osteosarcoma cell line - An in vitro analysis

Protective role of Gymnema sylvestre leaf extract on high sucrose diet-induced diabetic like phenotype, oxidative stress, reproductive fitness and longevity in Drosophila melanogaster

Protective role of Gymnema sylvestre leaf extract on high sucrose diet-induced diabetic like phenotype, oxidative stress, reproductive fitness and longevity in Drosophila melanogaster

Identification of bioactive compounds from the methanolic leaf extract of Gymenema Sylvestre

This study was aimed to analysis of potential bioactive phytoconstituents from the methanolic leaf extract of Gymnema sylvestre. Qualitative phytochemical screening of this plant confirms the presence of various secondary metabolites like steroids, alkaloids, saponins, tannins, ester, phenol and flavonoids. Gas chromatography mass spectroscopy analysis (GC-MS) also confirmed the presence of various phytochemical compounds with different chemical structure.

Silver nanoparticles from methanolic stem extract of <i>Gymnema sylvestre</i> and its characterisation studies

In this approach silver nanoparticles (AgNPs) were synthesised from Gymnema sylvestre stem extract. Gymnema sylvestre herb exhibits wide range of therapeutic properties. Phytochemical studies of this plant extract revealed the presence of alkaloids, flavonoids, tannins and glycosides. These phytoconstituents were responsible for the reduction of silver ions to silver nanoparticles. The bioreduced silver nanoparticles were characterised by UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), x-ray powder diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM). The free radical scavenging activity of stem extract of Gymnema sylvestre was also evaluated using DPPH assay. The free radical scavenging capacity of plant extract as well as silver nanoparticles derived from same plant extracts was done. DPPH radical scavenging analysis of Gymnema sylvestre stem extract was found to be around 56.25% which was lower than the leaf extract of Gymnema sylvestre. DPPH radical scavenging ability of Gymnema sylvestre stem extract was found to be 56.25%. Antimicrobial activity of these silver nanoparticles was examined through disc diffusion method. Zone of inhibition formed against silver nanoparticles at different concentrations 30, 60, 100 μg/μl was studied. Zone formation was found to be higher in gram negative microorganism at concentration of 100 μg/μl. This study showed that the less used stem part of Gymnema sylvestre which has an extensive therapeutic value can be effectively used for green synthesis of silver nanoparticles.

Silver nanoparticles from methanolic stem extract of <i>Gymnema sylvestre</i> and its characterisation studies

In this approach silver nanoparticles (AgNPs) were synthesised from Gymnema sylvestre stem extract. Gymnema sylvestre herb exhibits wide range of therapeutic properties. Phytochemical studies of this plant extract revealed the presence of alkaloids, flavonoids, tannins and glycosides. These phytoconstituents were responsible for the reduction of silver ions to silver nanoparticles. The bioreduced silver nanoparticles were characterised by UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), x-ray powder diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM). The free radical scavenging activity of stem extract of Gymnema sylvestre was also evaluated using DPPH assay. The free radical scavenging capacity of plant extract as well as silver nanoparticles derived from same plant extracts was done. DPPH radical scavenging analysis of Gymnema sylvestre stem extract was found to be around 56.25% which was lower than the leaf extract of Gymnema sylvestre. DPPH radical scavenging ability of Gymnema sylvestre stem extract was found to be 56.25%. Antimicrobial activity of these silver nanoparticles was examined through disc diffusion method. Zone of inhibition formed against silver nanoparticles at different concentrations 30, 60, 100 μg/μl was studied. Zone formation was found to be higher in gram negative microorganism at concentration of 100 μg/μl. This study showed that the less used stem part of Gymnema sylvestre which has an extensive therapeutic value can be effectively used for green synthesis of silver nanoparticles.