Gy Of Gamma Radiation Research Articles

Radioprotective potential of pomegranate peel extract against gamma irradiation-induced hazards

BackgroundWhile gamma irradiation’s damaging biological effects are well-established, the natural radioprotective agents from agricultural waste remain an underexplored area of significant potential.Aim of studyThis study was to investigate the novel use of pomegranate peel ethanol extract (PE) as a radioprotective agent against gamma radiation damage.MethodsWe pretreated Wistar rats with PE (100 mg/kg) for 14 days prior to 6 Gy gamma irradiation. We analyzed blood biochemicals, oxidative stress, and inflammatory markers. These included tests of red cell membrane integrity, lipid and protein oxidation, antioxidant enzyme levels, and cytokine profiles.ResultsThe study showed that PE demonstrated remarkable radioprotective effects across multiple parameters. Antioxidants were significantly enhanced, as evidenced by increased glutathione peroxidase activity (87.00 ± 6.11 mg/ml in PE-treated irradiated rats compared to 26.40 ± 1.21 mg/ml in irradiated controls). Oxidative damage was markedly reduced, with MDA levels dropping from 9.59 ± 0.24 nmol/ml in irradiated controls to near-control levels in PE-treated rats. Notably, PE treatment resulted in unprecedented maintenance of red blood cell membrane integrity post-irradiation. Furthermore, PE exhibited novel modulation of inflammatory cytokines, effectively reducing pro-inflammatory markers IL-6 and TNF-α while simultaneously boosting anti-inflammatory IL-4 and IL-10 levels. These multifaceted protective effects highlight PE’s potential as a comprehensive radioprotective agent.ConclusionThis study presents PE as an effective new natural radioprotective agent. Its protective effect is due to its high polyphenol content, which enhances antioxidant defenses, reduces oxidative damage, and prevents inflammation. The findings open new avenues for sustainable, cost-effective radioprotection strategies and demonstrate the potential for repurposing agricultural byproducts for critical health applications.

Protective role of melatonin against radiation-induced disruptions in behavior rhythm of zebrafish (danio rerio)

Ionizing radiation, as an increasingly serious environmental pollutant, has aroused widespread public concern. Melatonin, as an indole heterocyclic compound, is known to have anti-inflammatory and antioxidant effects. However, few studies have considered the comprehensive impact of melatonin on radiation damage. In this study, we used zebrafish as experimental materials and employed methods such as acridine orange staining, enzyme-linked immunosorbent assay (ELISA), video tracking for automated behavior analysis, microscope imaging, and real-time fluorescence quantitative analysis. Zebrafish embryos at 2 h post-fertilization (hpf) were treated under four different experimental conditions to assess their growth, development, and metabolic consequences. Our findings indicate that 0.10 Gy gamma radiation significantly augments body length, eye area, spine width, and tail fin length in zebrafish, along with a marked increase in oxidative stress (P < 0.05). Moreover, it enhances cumulative swimming distance, time, and average speed, suggesting elevated activity levels. We observed circadian rhythm phase shifts, peak increases, and cycle shortening, accompanied by abnormal expression of genes pivotal to biological rhythms, exercise, melatonin synthesis, apoptosis/anti-apoptosis, and oxidation/antioxidant balance. The inclusion of melatonin (1 × 10-5 mol/L MLT) ameliorated these radiation-induced anomalies, while its independent effect on zebrafish was negligible. Melatonin can regulate oxidative stress responses, hinders apoptosis responses, and reprogramming the expression of rhythm-related genes in zebrafish embryos after reprogramming radiation stimulation. Overall, our research highlights melatonin's critical role in countering the biological damage inflicted by gamma radiation, proposing its potential as a therapeutic agent in radiation protection.

Cognitive Effects of Simulated Galactic Cosmic Radiation Are Mediated by ApoE Status, Sex, and Environment in APP Knock-In Mice.

Cosmic radiation experienced during space travel may increase the risk of cognitive impairment. While simulated galactic cosmic radiation (GCRsim) has led to memory deficits in wildtype (WT) mice, it has not been investigated whether GCRsim in combination with genetic risk factors for Alzheimer's disease (AD) worsens memory further in aging mice. Here, we investigated the central nervous system (CNS) effects of 0 Gy (sham) or 0.75 Gy five-ion GCRsim or 2 Gy gamma radiation (IRR) in 14-month-old female and male APPNL-F/NL-F knock-in (KI) mice bearing humanized ApoE3 or ApoE4 (APP;E3F and APP;E4F). As travel to a specialized facility was required for irradiation, both traveled sham-irradiated C57BL/6J WT and KI mice and non-traveled (NT) KI mice acted as controls for potential effects of travel. Mice underwent four behavioral tests at 20 months of age and were euthanized for pathological and biochemical analyses 1 month later. Fecal samples were collected pre- and post-irradiation at four different time points. GCRsim seemed to impair memory in male APP;E3F mice compared to their sham counterparts. Travel tended to improve cognition in male APP;E3F mice and lowered total Aβ in female and male APP;E3F mice compared to their non-traveled counterparts. Sham-irradiated male APP;E4F mice accumulated more fibrillar amyloid than their APP;E3F counterparts. Radiation exposure had only modest effects on behavior and brain changes, but travel-, sex-, and genotype-specific effects were seen. Irradiated mice had immediate and long-term differences in their gut bacterial composition that correlated to Alzheimer's disease phenotypes.

Long-Term, Sex-Specific Effects of GCRsim and Gamma Irradiation on the Brains, Hearts, and Kidneys of Mice with Alzheimer's Disease Mutations.

Understanding the hazards of space radiation is imperative as astronauts begin voyaging on missions with increasing distances from Earth's protective shield. Previous studies investigating the acute or long-term effects of specific ions comprising space radiation have revealed threats to organs generally considered radioresistant, like the brain, and have shown males to be more vulnerable than their female counterparts. However, astronauts will be exposed to a combination of ions that may result in additive effects differing from those of any one particle species. To better understand this nuance, we irradiated 4-month-old male and female, wild-type and Alzheimer's-like mice with 0, 0.5, or 0.75 Gy galactic cosmic ray simulation (GCRsim) or 0, 0.75, or 2 Gy gamma radiation (wild-type only). At 11 months, mice underwent brain and heart MRIs or behavioral tests, after which they were euthanized to assess amyloid-beta pathology, heart and kidney gene expression and fibrosis, and plasma cytokines. Although there were no changes in amyloid-beta pathology, we observed many differences in brain MRIs and behavior, including opposite effects of GCRsim on motor coordination in male and female transgenic mice. Additionally, several genes demonstrated persistent changes in the heart and kidney. Overall, we found sex- and genotype-specific, long-term effects of GCRsim and gamma radiation on the brain, heart, and kidney.

Phenotypic characterization and genetic variability of Muthu Mullai mutants ofJasminum auriculatum for induction of homeotic mutants

An experiment was conducted during 2021–23 at Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu to create genetic variation in Jasminum auriculatum (Vahl.) by inducing mutation using physical and chemical means, through gamma rays and Ethyl Methane Sulphonate (EMS). The present study was designed to evaluate the M1V3 generation putative mutants of J. auriculatum ecotype Muthu Mullai generated through physical and chemical mutation with various dosages of gamma radiation and EMS. The study revealed that among the mutagen treated population, the plants subjected to 15 Gy gamma irradiation and 5 mM EMS recorded the maximum vegetative growth parameters including number of primary and secondary branches, internodal length, number of leaves, leaf length and leaf width and flower quality parameters namely days to flowering, number of flowering cymes/branch, flower bud length, corolla tube length, bud girth, flower diameter and 100 flower bud weight than control. Homeotic mutants were identified and isolated from the mutated population and this study further explored the reliability of the observed traits for making effective selection for crop improvement.

GSK-3β/Notch-1 Activation Promotes Radiation-Induced Renal Damage: The Role of Gallic Acid in Mitigation of Nephrotoxicity.

Despite the therapeutic advances in treating malignancies, the efficient radiotherapeutic approaches with deprived adverse reactions still represent a potential clinical inquiry. The current study aims to elucidate the role of gallic acid (GA) in modifying the hazardous renal cytotoxicity induced by acute exposure to radiation. The MTT test was used to evaluate the viability of Vero cells exposed to 2 Gy gamma radiation with or without incubation of GA. In an in vivo model, male Wistar rats were divided into four experimental groups (n = 6): Control, Irradiated (IRR, 5 Gy), GA (100 mg/kg, i.p.) + IRR, and Glycogen synthase kinase inhibitor (GSKI, 3 mg/kg, i.p.) + IRR. Based on the MTT toxicity assay, from 0 and up to 5 μM dosages of GA did not demonstrate any cytotoxicity to Vero cells. The optimal GA dose that could protect the cells from radiation was 5 μM. Furthermore, GA exerted a protective effect from gamma radiation on renal tissue as indicated by corrected renal functions, decreased LDH level in serum, and balanced oxidative status, which is indicated by decreased tissue contents of NOx and TBARS with a significant increase of reduced GSH. These outcomes were inferred by the upregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) expression. The overall molecular impact of radiation in damaging the renal tissue may be explained by modifying the upstream AKT activity and its downstream targets GSK-3β/Notch-1. Here, we concluded that the anticipated adverse reaction in the course of radiation exposure could be protected by daily administration of GA.

Gamma irradiation-engineered macrophage-derived exosomes as potential immunomodulatory therapeutic agents.

The modulation of macrophage polarization is a promising strategy for maintaining homeostasis and improving innate and adaptive immunity. Low-dose ionizing radiation has been implicated in macrophage immunomodulatory responses. However, studies on the relationship between exosomes and regulation of macrophage polarization induced by ionizing radiation are limited. Therefore, this study investigated the alterations in macrophages and exosomes induced by gamma irradiation and elucidated the underlying mechanisms. We used the mouse macrophage cell line RAW 264.7 to generate macrophages and performed western blot, quantitative reverse transcription-PCR, and gene ontology analyses to elucidate the molecular profiles of macrophage-derived exosomes under varying treatment conditions, including 10 Gy gamma irradiation. Exosomes isolated from gamma-irradiated M1 macrophages exhibited an enhanced M1 phenotype. Irradiation induced the activation of NF-κB and NLRP3 signaling in M1 macrophages, thereby promoting the expression of pro-inflammatory cytokines. Cytokine expression was also upregulated in gamma-irradiated M1 macrophage-released exosomes. Therefore, gamma irradiation has a remarkable effect on the immunomodulatory mechanisms and cytokine profiles of gamma-irradiated M1 macrophage-derived exosomes, and represents a potential immunotherapeutic modality.

Single-dose Administration of Recombinant Human Thrombopoietin Enhances Survival and Hematopoietic Reconstruction in Canines Irradiated with 3 Gy Gamma Radiation.

We conducted this study to investigate the radioprotective effects of recombinant human thrombopoietin (rhTPO) on beagle dogs irradiated with 3.0 Gy 60Co gamma rays. Fifteen healthy adult beagles were randomly assigned to a control group with alleviating care, and 5 and 10 μg/kg rhTPO treatment group. All animals received total-body irradiation using 60Co γ-ray source at a dose of 3.0 Gy (dose rate was 69.1 cGy/min). The treatment group received intramuscular injection of rhTPO 5 and 10 μg/kg at 2 h postirradiation, and the control group was administrated the same volume of normal saline. The survival rate, clinical signs, peripheral hemogram, serum biochemistry, and histopathological examination of animals in each group were assessed. Single administration of 10 μg/kg rhTPO at 2 h postirradiation promoted the recovery of multilineage hematopoiesis and improved the survival rate of beagles irradiated with 3 Gy 60Co γ rays. The administration of 10 μg/kg rhTPO alleviated fever and bleeding, reduced the requirement for supportive care, and may have mitigated multiple organ damage.

Protective Effects of Alpha-lipoic Acid, Resveratrol, and Apigenin Against Oxidative Damages, Histopathological Changes, and Mortality Induced by Lung Irradiation in Rats.

This study investigated the protective effects of three antioxidants on radiationinduced lung injury. Oxidative stress is one of the key outcomes of radiotherapy in normal tissues. It can induce severe injuries in lung tissue, which may lead to pneumonitis and fibrosis. Recently, interest in natural chemicals as possible radioprotectors has increased due to their reduced toxicity, cheaper price, and other advantages. The present study was undertaken to evaluate the radioprotective effect of Alpha-lipoic Acid (LA), Resveratrol (RVT), and Apigenin (APG) against histopathological changes and oxidative damage and survival induced by ionizing radiation (IR) in the lung tissues of rats. First, the lung tissue of 50 mature male Wistar rats underwent an 18 Gy gamma irradiation. Next, the rats were sacrificed and transverse sections were obtained from the lung tissues and stained with hematoxylin and eosin (H and E) and Mason trichrome (MTC) for histopathological evaluation. Then, the activity of Glutathione peroxidase (GPx), Superoxide Dismutase (SOD), and Malondialdehyde (MDA) was measured by an ELISA reader at 340, 405, and 550 nm. Based on the results of this study, IR led to a remarkable increase in morphological changes in the lung. However, APG, RVT, and LA could ameliorate the deleterious effects of IR in lung tissue. IR causes an increase in GPX level, and APG+IR administration causes a decrease in the level of GPX compared to the control group. Also, the results of this study showed that RVT has significant effects in reducing MDA levels in the short term. In addition, compared to the control group, IR and RVT+IR decrease the activity of SOD in the long term in the lung tissues of rats. Also, the analysis of results showed that weight changes in IR, LA+IR, APG+IR, and control groups were statistically significant. APG and RVT could prevent tissue damage induced by radiation effects in rat lung tissues. Hence, APG, LA, and RVT could provide a novel preventive action with their potential antioxidant anti-inflammatory properties, as well as their great safety characteristic.

Exploring of Lethal Dose (LD50) Using Gamma Rays and Its Impact on Seed Germination and Seedling Survival in Stevia rebaudiana Bertoni cv. CIM-Madhu

Stevia rebaudiana Bertoni an natural alternative to sugar and is known for production of sweet glycosides. The impact of gamma radiations in developing new stevia mutants was carried out at Department of Horticulture, University of Agricultural Sciences, GKVK, Bengaluru. Seeds of stevia cv. CIM-Madhu were collected and irradiated with various dosages of gamma rays viz. 50, 100, 200, 300, 400, 500 and 600 Gy to assess the LD50 value. The maximum germination (65%) was seen in the non-mutagenized control group followed by 61 percent in treatment comprising of 50 gy gamma irradiation with 6.15 per cent reduction in germination over the control. The maximum survival percentage was recorded in non-mutagenized control group (89%). Non-mutagenized control group took significantly lesser number of days (12 days) for germination and the treatment comprising of 200 Gy and 500 Gy took maximum number of days for germination (20 days). LD50 a dose that causes 50% mortality to the seeds was found at 150 Gy. This LD50 dose can be used as reference for initiating mutation breeding in other cultivars of stevia.

Comparative Analysis of Physical and Chemical Mutagenesis in Chrysanthemum cv. 'Candid': Assessing Genetic Variation and Breeding Potential.

In this study, we developed a mutagenesis protocol specifically designed for chrysanthemum cv. "Candid" in order to introduce genetic variation. By subjecting chrysanthemum shoots to different doses of physical and chemical mutagens, we successfully generated a total of 24 mutants, each with unique genetic compositions. We observed that the mortality rate was lowest when the shoots were exposed to 10 Gy gamma irradiation and 1.00% EMS. To assess the diversity and relatedness among the mutants, we employed RAPD and SSR markers. The combination of these markers allowed us to construct a dendrogram that effectively categorized the mutant population into distinct clusters based on the specific mutagen treatments. Interestingly, the mutants induced by 10 Gy gamma irradiation exhibited greater genetic diversity in terms of flower colors. On the other hand, mutants created with 1.00% EMS displayed a higher level of variation and yielded more viable mutants. To determine the optimal markers for studying genetic diversity, we analyzed the polymorphic information content (PIC) of different markers. Among the tested markers, OPA-07 (RAPD) and JH47 (SSR) showed the highest PIC values, indicating their effectiveness in capturing genetic variability within the mutant population. Conversely, the PIC values of OPD-07 and JH20 demonstrated the lowest among the markers tested. Our results revealed a percentage of polymorphism ranging from 81.81% to 100% for RAPD markers and 66.66% to 100% for SSR markers. These findings indicate that physical mutation induced by 10 Gy gamma irradiation can be clearly distinguished from chemical mutation induced by EMS at concentrations of 1% and 0.75% in chrysanthemum cv. "Candid.″ Overall, this study provides valuable insights into the genetic composition of the generated mutants and highlights their potential for enhancing chrysanthemum-breeding programs. The identified markers, particularly, OPA-07 and JH47, can serve as valuable tools for future studies aimed at exploring and exploiting the genetic diversity within the chrysanthemum population.

Profiles of Semi-Polar Metabolites from Leaves of In Vitro- Derived Plants of Indonesian Pepper Varieties (Capsicum annuum) after Gamma Irradiation Treatments

Gamma irradiation has been widely utilized for mutation breeding as it induces random mutations in plant cells. Due to limitations on other breeding approaches, including cross-breeding and transgenic plants, gamma irradiation-induced mutation breeding has regained its popularity among breeders and scientists. Untargeted metabolomics analysis can be used to profile any perturbation between untreated samples and treated sample groups without having prior knowledge of particular metabolites, including semi-polar metabolites, which cover phenolic acids, flavonoids, glycosylated steroids, alkaloids, and other glycosylated species. This study aimed to profile semi-polar metabolite modification in leaves of plants derived from in vitro cultures after gamma irradiation treatments in Indonesian chili pepper varieties. Seeds of Laris and Kopay were exposed to gamma-ray doses (0, 100, 200, 300, 400, and 500 Gy), after which they were germinated and cultivated in vitro. Plantlets were acclimated in the greenhouse and leaves were collected for metabolite analysis at 80 days after the germination of irradiated seeds. Semi-polar metabolites from leaves were extracted using methanol and the extracts were subjected to LC-MS analysis. Results showed that the composition and levels of semi-polar metabolites of Laris 100, 200, 300, 400, and 500 Gy were similar to Laris 0 Gy (control; unirradiated seeds), although several abundancies of that of 200 Gy gamma irradiation dose were different than control. Except at dose 400 Gy, Kopay at 0 Gy, 200 Gy, and 300 Gy also showed similar metabolite profiles, indicating that gamma-ray doses did not induce mutation at genes regulating metabolite biosynthetic pathways. In contrast, Kopay 400 Gy showed low levels of terpenoids and flavonoids, indicating that a 400 Gy dose of gamma ray may affect the upstream part of the shikimate biosynthetic pathway, resulting in low levels of precursors at the upstream biosynthetic pathway of terpenoids and flavonoids. Hence, the accumulation of terpenoids and flavonoids was very low. These findings provide insights into the effect of gamma irradiation for mutation breeding that may be important for future pepper breeding programs.

Study of The Structural, Optical, and Morphological Properties of Sno2 Nanofilms under the Influence of Gamma Rays

This study reports the fabrication of tin oxide (SnO2) thin films using pulsed laser deposition (PLD). The effect of 60Co (300, 900, and 1200 Gy) gamma radiation on the structural, morphological, and optical features is systematically demonstrated using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and ultraviolet-visible light analysis (UV-Vis), respectively In XRD tests, the size of the crystallites decreased from 45.5 to 40.8 nm for the control samples and from 1200 Gy to 60Co for the irradiated samples. Using FESEM analysis, the particle diameter revealed a similar trend to that attained using XRD; in particular, the average diameters were 93.8 and 79.9 nm for the samples mentioned above. A similar profile was observed for the AFM analysis in which an increase in the radiation dose from 300 to 1200 Gy resulted in a decrease in the RMS values from 74.6 to 32.25 nm. Contrariwise, the calculated optical band gap demonstrated an increasing profile where optical band gaps of 3.08 and 3.18 eV were acquired for control and samples irradiated with 900 Gy. However, the attained optical band gap was further increased to 3.24 eV due to the 60Co gamma radiation increment to 1200 Gy.

Effect of ionizing radiation on thermal and mechanical properties of filled-epoxy adhesives

Stave assemblies at the Large Hadron Collider and other high-energy physics experiments require radiation-resistant adhesives composed of light elements with high thermal conductivity and low interfacial resistance. Porous carbon foams are currently bonded to carbon fiber faceplates with graphite-filled epoxy. Low-viscosity epoxies are used to increase the amount of filler in order to double the thermal conductivity without impacting the properties of the material under irradiation. Promising new adhesive formulations showed no degradation in thermal conductivity after exposure to a fluence of 1016 n/cm2 (>0.1 MeV neutrons) or 10 Gy gamma radiation, but showed variable degradation, compared to the currently used adhesive, after exposure to a fluence of 1015 p/cm2 (1 MGy, 67.5 MeV protons). Adhesives with more than double the irradiated thermal conductivity of the baseline material showed marked improvement in thermal transport in graphite/epoxy/foam/epoxy/graphite structures based on thermal diffusivity measurements. Silicon carbide particles can be added to graphite to stiffen the modulus of the filled epoxy. When spherical AlN was used as a filler, the degradation in thermal conductivity under proton irradiation varied based on the size of the ceramic filler. Options for improving the thermal performance of irradiated adhesives for high-energy physics experiments are discussed.

New quinazoline sulfonamide derivatives as potential anticancer agents: Identifying a promising hit with dual EGFR/VEGFR-2 inhibitory and radiosensitizing activity

Herein, we report the synthesis of a series of new quinazoline sulfonamide conjugates 2–16 and their evaluation as potential anticancer agents via dual targeting of EGFRT790M and VEGFR-2. The newly synthesized compounds were designed based on the structure requirements of the target receptors and were confirmed using spectral data. The compounds were evaluated for their cytotoxicity against four cancer cell lines (HepG2, MCF-7, HCT116 and A549) using MTT assay. The most active compounds were further evaluated for their inhibitory activity against EGFRT790M and VEGFR-2. Compound 15 showed the most significant cytotoxic activity with IC50 = 0.0977 µM against MCF-7 and the most potent inhibitory activity against both EGFR and VEGFR with IC50 = 0.0728 and 0.0523 µM, respectively. Compound 15 was able to induce apoptosis in MCF-7 cells and cell cycle arrest at the G2/M phase. The relative safety profile of 15 was assessed using HEK-293 normal cell line and an ADMET profile was carried out. Radiosensitizing evaluation of 15 proved its significant ability to sensitize the cancer cell to the effect of radiation after being subjected to a single dose of 8 Gy gamma irradiation. Molecular docking studies revealed that 15 could bind to the ATP-binding site of EGF and VEGF receptors, inhibiting their activity.

Reparative potential of mesenchymal stem cells and platelet-rich plasma on irradiated submandibular glands of male albino rats

ObjectiveTo appraise and compare the reparative role of bone marrow-mesenchymal stem cells (BM-MSCs) and platelet-rich plasma (PRP) against irradiation damage on albino rats’ submandibular gland. DesignSeventy four male albino rats were used, one for BM-MSCs harvesting, 10 for PRP preparation, seven as control group (Group 1). The remaining 56 rats were subjected to single dose (6 Gy) gamma irradiation and were divided into equal four groups; (Group 2): received no treatment, (Group 3): each rat was injected with 1 × 105 BM-MSCs, (Group 4): each rat was injected with 0.5 ml/kg PRP, and (Group 5): each rat was injected with 1 × 105 BM-MSCs and 0.5 ml/kg PRP. Each group was further subdivided into two subgroups in which rats sacrificed after one and two weeks from irradiation. Any structural changes were examined histopathologically, immunohistochemically using proliferating cell nuclear antigen (PCNA) and CD31 primary antibodies and histochemically using picrosirius red (PSR) stain, then analyzed statistically. ResultsHistopathological examination of Group 2 showed atrophied acini, with nuclear changes and signs of degeneration in duct systems. Treated groups revealed signs of regeneration in form of uniform acini and regenerated duct systems especially in Group 5 and in a time depended manner. Immunohistochemical examination revealed increased immunoexpression of PCNA and CD31, while histochemical examination showed decreased PSR in all treated groups in relation to the irradiated group and this was proved statistically. ConclusionsBM-MSCs and PRP are effective as treatment for irradiation-induced submandibular gland damage. However, the combined therapy is recommended over each one separately.

Different damaging effects of volatile anaesthetics alone or in combination with 1 and 2 Gy gamma-irradiation in vivo on mouse liver DNA: a preliminary study.

As the number of radiotherapy and radiology diagnostic procedures increases from year to year, so does the use of general volatile anaesthesia (VA). Although considered safe, VA exposure can cause different adverse effects and, in combination with ionising radiation (IR), can also cause synergistic effects. However, little is known about DNA damage incurred by this combination at doses applied in a single radiotherapy treatment. To learn more about it, we assessed DNA damage and repair response in the liver tissue of Swiss albino male mice following exposure to isoflurane (I), sevoflurane (S), or halothane (H) alone or in combination with 1 or 2 Gy irradiation using the comet assay. Samples were taken immediately (0 h) and 2, 6, and 24 h after exposure. Compared to control, the highest DNA damage was found in mice receiving halothane alone or in combination with 1 or 2 Gy IR treatments. Sevoflurane and isoflurane displayed protective effects against 1 Gy IR, while with 2 Gy IR the first adverse effects appeared at 24 h post-exposure. Although VA effects depend on liver metabolism, the detection of unrepaired DNA damage 24 h after combined exposure with 2 Gy IR indicates that we need to look further into the combined effects of VA and IR on genome stability and include a longer time frame than 24 h for single exposure as well as repeated exposure as a more realistic scenario in radiotherapy treatment.

Brain DNA damaging effects of volatile anesthetics and 1 and 2 Gy gamma irradiation in vivo: Preliminary results

Although both can cause DNA damage, the combined impact of volatile anesthetics halothane/sevoflurane/isoflurane and radiotherapeutic exposure on sensitive brain cells in vivo has not been previously analyzed. Healthy Swiss albino male mice (240 in total, 48 groups) were exposed to either halothane/sevoflurane/isoflurane therapeutic doses alone (2h); 1 or 2gray of gamma radiation alone; or combined exposure. Frontal lobe brain samples from five animals were taken immediately and 2, 6, and 24h after exposure. DNA damage and cellular repair index were analyzed using the alkaline comet assay and the tail intensity parameter. Elevated tail intensity levels for sevoflurane/halothane were the highest at 6h and returned to baseline within 24h for sevoflurane, but not for halothane, while isoflurane treatment caused lower tail intensity than control values. Combined exposure demonstrated a slightly halothane/sevoflurane protective and isoflurane protective effect, which was stronger for 2 than for 1gray. Cellular repair indices and tail intensity histograms indicated different modes of action in DNA damage creation. Isoflurane/sevoflurane/halothane preconditioning demonstrated protective effects in sensitive brain cells in vivo. Owing to the constant increases in the combined use of radiotherapy and volatile anesthetics, further studies should explore the mechanisms behind these effects, including longer and multiple exposure treatments and in vivo brain tumor models.

Selection of M3 mutant strains from 200 gy gamma irradiation on Cempo Ireng

Climate change is a serious issue that can disrupt crop production and pest resistance, especially in rice crops. To tackle climate change, plant breeding can be employed to develop new characteristics that are resistant, using physical mutagenesis like gamma irradiation. Cempo Ireng is one type of rice in Indonesia with plant properties such as tall plant, long harvesting periods, and susceptibility to brown planthopper attacks. The research aims to obtain information and select M3 mutant strains with properties of shorter plants, early harvesting, and brown planthopper resistance. The study was conducted from February-July 2022 in Klaten Regency, Central Java, Indonesia. The research material consisted of 3 mutant strains of black rice M3(GH8'), M3(GH51'), and M3(GH52'), with the control variety of Cempo Ireng. The experimental design used was a Randomized Complete Block Design without replication. Data were analyzed descriptively and used t-test, at a significance level of 5%. The results showed that M3 mutant black rice had better growth and yield components than the control plant. Brown planthopper attacks of M3 mutant strains decreased to 24% compared to the control.

Evaluation of the optimum threshold of gamma-ray for inducing mutation on Polianthes tuberosa cv. double and analysis of genetic variation with RAPD marker

Purpose This experiment aimed to investigate the effect of gamma irradiation on morpho-physiological characteristics and molecular-induced variations in Polianthes tuberosa L. Methods Experiments were designed according to a completely randomized design with eight different gamma-ray doses (0, 20, 30, 40, 50, 60, 70, and 80 Gy) via a source of cobalt-60 with three replications. Some morpho-physiological characteristics of tuberoses were screened and evaluated at the end of the flower growth and development phases. The RAPD-PCR molecular marker technique was further used to identify the mutants of phenotypic variation flowers. Results Results indicated that the effect of different levels of γ-rays on some morphological and physiological traits was significant as the gamma-ray level was increased up to 50 Gy. The doses higher than 50 Gy were found to cause stand or no growth. The 50 Gy gamma irradiation reduced germination by 70.59%, germination rate by 66.36%, dry weight by 88.15%, fresh weight by 87.41%, flowering stem height (cm) by 69.22%, leaf area (cm2) by 57.35%, leaf number by 34.41%, chlorophyll content (mg g−1 FW) by 44.79%, number of florets by 92.57%, spike height (cm) by 27.80%, bulblet number by 32.57%, and bulblet diameter (mm) by 30.21%. On the contrary, gamma radiation at 50 Gy increased relative water content (%) and electrolyte leakage (ds m−1) by 41.27 and 237.65%, respectively. The results also showed that bulbs treated with 20 Gy gamma ray had the highest germination percentage and dry weight. The RAPD analysis indicated that among 10 primers tested, nine primers showed clear bands as the highest number of amplified fragments (90) was related to the OPM13 primer and the lowest number (40) to the OPM10 primer. However, the DNA polymorphism was dose-dependent. Conclusion Overall results showed that although the plant morphology was changed with gamma-ray level, no changes occurred in tuberose color.