Gutta-percha Research Articles

Management of Non-Vital Open Apex in A Lateral Incisor Using Calcium Hydroxide and Reverse GP Cone Cold Lateral Compaction in A General Practice Setting

Although dental trauma is poorly managed by general dental practices, it is important to follow appropriate steps to achieve optimal results. This case report outlines the management of non-vital immature teeth with calcium hydroxide and reverse Gutta Percha (GP) cone cold lateral compaction to achieving endodontic success. Even though it is a disputed technique, if proper conditions are provided, the root development continues to preserve a functionally fit and sound tooth on the arch of a young adolescence, avoiding extensive surgical and prosthetic procedures to replace a potentially compromised tooth. This case report highlights the beneficial effects of both calcium hydroxide and Chlorhexidine-dygluconate (CHX), which are far to be obsolete in the field of apexification or even regenerative dentistry as a whole.The 11 years old male presents post-traumatic necrosis in the upper left lateral incisor (tooth 2.2) which is dressed with calcium hydroxide, achieving the disinfection of the root canal, remission of the apical periodontitis and complete root formation 1 year later. The importance of the use of biocompatible materials as calcium hydroxide and CHX are emphasized in this case in which, although, no modern regenerative procedures and materials were used, the results are more than satisfactory. This report will look into the management of a non-vital immature lateral incisor with a reverse GP cone lateral compaction to achieving a successful apical and canal seal radiographically.

Evaluation of Antibacterial Properties of Organic Gutta-percha Solvents and Synthetic Solvents Against Enterococcus faecalis.

ABSTRACTBackground:The use of solvents is necessary to remove remnants of filling materials within dentinal tubules to allow penetration of irrigating solution within the tubules.Aim and Objectives:We aimed at determining the antibacterial effect of various gutta-percha (GP) solvents against Enterococcus faecalis (E. faecalis).Materials and Methods:An in vitro study was conducted by measuring the zone of inhibition using the disk diffusion method. The test organism used for the study was E. faecalis, and the solvents were divided into five groups: eucalyptus oil, chloroform, turpentine oil, xylene, and orange oil. About 500 μL of the suspension was spread over the agar plates, and the empty sterilized disks were impregnated with 10 μL of pure essential oils (EOs). The inoculated plates were incubated at 37°C for 18 to 24 h. The antimicrobial activity of each solvent was expressed and measured in terms of the mean diameter of the zone of inhibition (in mm) produced by each solvent at the end of the incubation period. ANOVA was used for intergroup comparison. The P-value of <0.05 was considered significant.Results:The mean zone of inhibition for E. faecalis was 24.00 ± 1.21 for eucalyptus oil, 16.30 ± 0.92 for chloroform, 26.50 ± 1.24 for turpentine oil, 13.70 ± 1.26 for xylene, and 19.80 ± 1.32 for orange oil. The difference between the groups was statistically significant (P < 0.001).Conclusion:This study demonstrated that the use of turpentine oil during endodontic retreatment significantly reduced the levels of E. faecalis as compared with other solvents.

IN VITRO EVALUATION OF THE ANTIMICROBIAL EFFECT OF ENDOSEQUENCE BC AND AH PLUS ROOT CANAL SEALERS ON ENTEROCCOCUS FAECALIS

INTRODUCTION: Remaining microorganisms after chemo-mechanical cleaning and disinfection may be eliminated or rendered harmless by entombing them through complete obturation with gutta percha points and sealer. Adequate antimicrobial effect is of optimum importance to achieve root canal treatment success.OBJECTIVES: The aim of this study was to investigate the antimicrobial potential of two types of sealers against Enterococcusfaecalis present in dentinal tubules, over a 30 day period.MATERIALS AND METHODS: Cells of Enterococcus faecalis were introduced into the dentinal tubules by incubation in brain-heart infusion broth for 3 weeks. AH Plus and Endosequence BioCeramic sealer (BC sealer) were coated on the root canal wall of the dentin specimens and cold lateral obturation technique was preformed, specimens were then stored for one, seven, and 30 days at 37°C. At the end of each incubation period, dentin powder was collected by sequential removal of dentin by the use of peeso reamers throughout the sample thickness, total bacterial count was assessed by Colony forming units (CFU) count.RESULTS: No significant difference was observed in the total bacterial count among the three groups in day one. AH Plus showed statistically significant lower bacterial counts in comparison to the positive control group in day 7 and day 30, while Endosequence BC showed a statistically significant lower bacterial count in comparison to the positive control group at day 30.CONCLUSIONS: AH plus exhibited a stronger antibacterial affect after setting and therefore is more effective than Endosequence BC in eliminating Enterococcus faecalis from the root canal system. In addition, both sealers lose their antibacterial effect with time.

COMPARATIVE EVALUATION OF TENSILE BOND STRENGTH OF ENDODONTIC SEALERS TO DENTIN AND GUTTA PERCHA - AN IN-VITRO STUDY

Introduction: The root canal therapy is done with the intention to seal it off from any fluid leaks, thus enhancing the longevity of the therapy. The seal between the obturating material and the dentinal walls of the root surface is done by the endodontic sealer. The sealer is designed in such a way that it has to mould itself with the curves and follow the shapes of the root dentinal wall.&#x0D; Aims: To compare in-vitro the tensile bond strength of four different endodontic sealers to root dentin and Gutterpercha.&#x0D; Methods: Thirty six single ‑ rooted canine and premolar teeth with total apical formation were selected. Working length was determined after the crown portion was cut. BMP was done. Teeth were grouped as Group Ⅰ: MTA‑ based sealer, Group Ⅱ: Bioceramic Sealer, Group Ⅲ: Calcium based sealer, Group Ⅳ: Epoxy resin based (AH Plus sealer). Sealers were manipulated according to the manufacturer protocol. Obtuartion was done using 4% gutta ‑ percha. Later tensile strength test was done on the root slices of 2 mm thickness using universal testing machine. 6 mm in diameter Aluminum cylinders, were stabilized on the gutta–percha with small amounts of wax and were filled with one of the sealers. After setting each sealer, the drops of wax were removed and the tensile bond strengths of all the samples were measured using universal testing machine. The data was analysed using Tukey tests and ANOVA.&#x0D; Results: Among the 4 groups highest bond strength was found in Group Ⅱ (BioRootTMRCS) (P &lt; 0.05) and the least was Group Ⅰ (MTA Fill apex).&#x0D; Conclusion: Bioceramic sealer’s tensile bond strength was highest followed by AH26, Sealapex and MTA‑ based sealer.&#x0D; Keywords: Bond strength; BioRoot TMRCS Sealer; MTA Fill apex; AH Plus Sealer, Sealapex, tensile strength.

Graphene nanoplatelets embedded polymer: An efficient endodontic material for root canal therapy

The design and preparation of clinically relevant endodontic obturating material for root canal therapy is a great challenge. For the first time, we report a new polymer nanocomposite which was prepared by using reversible addition-fragmentation chain-transfer (RAFT) polymerization of methacrylic acid and methylene glycol dimethacrylate. The polymer was embedded with reduced graphene oxide nanoplatelets (rGO). These graphene nanoplatelets were embedded in the polymers (GNPs) have shown the tensile strength (27‐–36%) and the elongation at break 2.1 – 3.1% is quite similar to the commercial gutta percha (GP-C). Atomic force micrograph provided interesting information related to scattering of rGO flakes in GNPs and the surface of GNP contains crystalline spikes of height varied between 0.95 and 1.26 μm. These spikes improved the adhesion of GNPs to bio-interface. The GNPs were 95% more effective in inhibiting bacterial colonization without disturbing the nearby cell integrity compared to commercial GP. It was found that the GNPs after incubation of 24 h at 37 °C, the radius of the inhibition zone was 6.8 mm and 4.3 mm for E.coli and S. aureus, respectively indicating better effective antibacterial activity than the GP-C. This work offers biocompatible, better adhesive and antibacterial endodontic obturating material for future root canal therapy.

The Effect of Ultrasonic Activation of Two Different Sealers on the Fracture Resistance of Obturated Root Canals

The aim of the current study is to evaluate the effect of ultrasonic activation of two sealers: EndoSequenceBC and AH Plus on the fracture resistance of obturated roots.Materials and Method: Forty eight mandibular first premolars were decoronated to a length of 13 mmfrom the apex. The roots were divided into two groups (24 samples) to be obturated with gutta percha usingeither EndoSequence BC(Group I) or AH Plus (Group II) sealers. Each group was further subdivided intotwo subgroups (n=12). Subgroups IA and IIA were obturated without ultrasonic activation of the sealers.Subgroups IB and IIB were obturated with ultrasonic activation of the sealers. The activation of the sealerswas performed using size 15 ultrasonic K-file for a total of 20 seconds 2 mm short of the working length. Thesamples were subjected to fracture resistance test using a universal testing machine at a across head spead of0.5 mm/min. The data were analyzed using One way ANOVA and Tukey tests at a significant level of 0.05.Results: The fracture resistance mean values of the EndoSequence BC subgroups with and without activationwere significantly higher than those of AH Plus sealer (P<0.05).Conclusion: The ultrasonic activation of both sealers did not enhance the fracture resistance of obturatedroots.

Effect of Inlays, Onlays and Endocrown Cavity Design Preparation on Fracture Resistance and Fracture Mode of Endodontically Treated Teeth: An In Vitro Study.

To evaluate the fracture resistance and failure modes of endodontically treated mandibular molars restored with different designs of inlays, onlays and endocrowns. Extracted mandibular third molars (n = 180) were used. An access cavity was prepared on the occlusal surface of each tooth and the roots were obturated with gutta percha. All specimens were randomly divided into 6 groups (n = 30/group) according to the cavity design and the restoration material used. C: control group without access cavity preparation. IE: MOD inlay preparation with EverX Posterior (GC Europe) in the pulp chamber. IG: MOD inlay preparation with G-aenial Universal Flo (GC America) in the pulp chamber. OE: onlay preparation with EverX Posterior (GC Europe) in the pulp chamber. OG: onlay preparation with G-aenial Universal Flo (GC America) in the pulp chamber. EC: endocrown with an empty pulp chamber. All restorations were fabricated with CAD/CAM system using CERASMART® (GC Dental products Europe, Belgium) CAD/CAM blocks. Specimens were thermal-cycled and were subjected to a compressive load applied at 30° angle relative to the long axis of the tooth with a universal testing machine. Results were statistically analyzed by ANOVA followed by Tukey post hoc tests. Chi-square test and Fisher Exact tests were used for the comparisons among groups. The mean fracture strength was significantly different between the groups (p < 0.001); it was significantly highest for intact teeth, followed by endocrowns (p = 0.021). The strength was significantly lower for inlays (with G-aenial Universal Flo and EverX Posterior), intermediate for onlays with EverX Posterior followed by onlays with G-aenial Universal Flo. Endocrowns exhibited higher fracture resistance than other tested composite resin groups. Endocrowns and onlays showed a more favorable failure mode than inlay restorations.

Guttaflow Bioseal as Monocone Obturation Technique: A Scanning Electron Microscopy Study

INTRODUCTION: This study compared the obturated surface area, the extrusion of root filling material beyond the apical foramen and the duration of obturation procedure in single-rooted mandibular premolar using monocone obturation technique. MATERIALS AND METHODS: The root canal of twenty single-rooted mandibular premolars were prepared, and then divided into two groups; Group 1 (GuttaFlow Bioseal (GFB) and a gutta-percha (GP) cone) and Group 2 (RoekoSeal Automix root canal sealer and a GP cone). The obturation procedure was timed and the obturation radiograph was taken after the procedure. The roots were sectioned perpendicularly and the surfaces were observed under scanning electron microscope. The images were transferred to the SketchAndCalc Area Calculator software for evaluation of the obturated surface area. RESULTS: The median score of obturated surface area in Group 1 and 2 at the apical was 86.51 and 83.00, at the middle was 90.48 and 87.35 and at the coronal was 93.00 and 83.39, respectively. The extrusion of root filling material between two groups did not show statistically significant difference. The mean duration of obturation in Group 1 and 2 was 149.50 and 137.60, respectively. CONCLUSIONS: The obturated surface area at the apical and middle root regions in Group 1 and 2 was comparable but at the coronal region, Group 1 showed 11.5% better coverage. The extrusion of root filling material in Group 1 and 2 was equivalent. Obturation procedure in Group 1 took 8.6% longer than in the Group 2.

Micro-computed tomographic evaluation of single-cone obturation with three sealers.

ObjectivesThis study used micro-computed tomography (µCT) to compare voids and interfaces in single-cone obturation among AH Plus, EndoSequence BC, and prototype surface pre-reacted glass ionomer (S-PRG) sealers and to determine the percentage of sealer contact at the dentin and gutta-percha (GP) interfaces.Materials and MethodsFifteen single-rooted human teeth were shaped using ProTaper NEXT size X5 rotary files using 2.5% NaOCl irrigation. Roots were obturated with a single-cone ProTaper NEXT GP point X5 with AH Plus, EndoSequence BC, or prototype S-PRG sealer (n = 5/group).ResultsThe volumes of GP, sealer, and voids were measured in the region of 0–2, 2–4, 4–6, and 6–8 mm from the apex, using image analysis of sagittal µCT scans. GP volume percentages were: AH Plus (75.5%), EndoSequence BC (87.3%), and prototype S-PRG (94.4%). Sealer volume percentages were less: AH Plus (14.3%), EndoSequence BC (6.8%), and prototype S-PRG (4.6%). Void percentages were AH Plus (10.1%), EndoSequence BC (5.9%), and prototype S-PRG (1.0%). Dentin-sealer contact ratios of AH Plus, EndoSequence BC, and prototype S-PRG groups were 82.4% ± 6.8%, 71.6% ± 25.3%, and 70.2% ± 9.4%, respectively. GP-sealer contact ratios of AH Plus, EndoSequence BC, and prototype S-PRG groups were 65.6% ± 29.1%, 80.7% ± 25.8%, and 87.0% ± 8.6%, respectively.ConclusionsPrototype S-PRG sealer created a low-void obturation, similar to EndoSequence BC sealer with similar dentin-sealer contact (> 70%) and GP-sealer contact (> 80%). Prototype S-PRG sealer presented comparable filling quality to EndoSequence BC sealer.

Evaluation of surface analysis of gutta-percha after disinfecting with sodium hypochlorite, silver nanoparticles, and chitosan nanoparticles by atomic force microscopy: An in vitro study.

Aims and Objectives:The purpose of this study is to evaluate the surface topography of gutta-percha (GP) after disinfecting with sodium hypochlorite (NaOCl) (5.25%), silver nanoparticles (AgNPs) (70 µg/ml), and chitosan nanoparticles (ChNPs) (1.5 mg/ml) by atomic force microscopy (AFM).Materials and Methods:Forty GP cones were taken in this in vitro study. These samples were divided into four different groups such as Group I – control group (untreated GP Points) and Group II, III, and IV were treated with 5.25% NaOCl, 70 µg/ml AgNPs, and 1.5 mg/ml ChNPs, respectively. The surface topography analysis of the samples was performed using AFM.Statistical Analysis:Root mean square (RMS) and surface roughness parameters were used to compare the structure of GP points with contact mode imaging. These values were tested by IBM SPSS-20.0 version statistical software using one-way ANOVA and post hoc (Tukey's honestly significant difference) tests. They were considered statistically significant when P < 0.05.Results:The RMS and surface roughness values are significantly higher for NaOCl group (5.25% NaOCl) when compared with AgNPs group (70 µg/ml AgNPs) and ChNPs group (1.5 mg/ml ChNPs).Conclusion:This study has shown more surface topography deterioration of GP treated with NaOCl and lesser deterioration with AgNPs and ChNPs which affects postoperative prognosis.

Endodontic retreatment of a mandibular canine with two roots and two canals: a case report

Background: The knowledge of root canal anatomy is considered essential in the diagnoses and treatment of endodontic cases to assure successful outcomes. Although the mandibular canine is known to be a single- rooted tooth with a single canal, variations can be seen. However, the incidence of a mandibular canine having two roots and two canals is extremely rare. Case Presentation: This article reports a case of non-surgical retreatment of mandibular canine, with two roots and two canals, presented to the clinic with a periapical lesion. Chloroform and H-files were used to remove previous gutta percha, canals were shaped with a profile rotary system, and irrigated with 2.5% sodium hypochlorite. The canals were then obturated with gutta percha and AH Plus sealer. Conclusion: Clinicians must use all the available tools to help them detect anatomical variations to manage their cases successfully.

Antimicrobial efficacy of acacia Nilotica (Babul) extract and its effectiveness in disinfecting gutta percha cones - An in vitro study.

Gutta-percha cones used directly from the manufacturer's sealed packs harbour micro-organisms. During clinical use, the chances of contamination of gutta-percha increases. Hence, disinfection of gutta-percha cones before use is essential to prevent canal recontamination. To evaluate the antimicrobial efficacy of babul against chemical disinfectants, and its effectiveness in disinfecting gutta-percha cones. The extract of babul was used to assess its antimicrobial activity against E-faecalis, S. aureus and C. albicans using the agar diffusion method. A total of 120 cones acquired from freshly opened manufacturers' pack were cut into three equal parts and divided into group I which was directly placed into a nutrient broth, group II was clinically contaminated with gloves for 30 s and placed into a nutrient broth, and the third part was clinically contaminated with gloves and decontaminated for 5 min using the experimental disinfectants to check for the presence of turbidity. The data were analysed by an Unpaired t-test and Chi-square test at a significance level of P < 0.05 using software SPSS 20. Two percent chlorhexidine showed maximum antibacterial action against E. faecalis and S. aureus followed by babul extract. It was also found more efficacious in decontaminating gutta-percha cones followed by babul and 3% sodium hypochlorite in 5 min. Babul herbal extract is indeed effective in decontaminating gutta-percha cones and is a better alternative to chemical disinfectants.

Microleakage of different temporary luting agents used for cementing provisional restorations on custom cast posts and cores

This study aimed to compare the effect of four temporary luting agents on preventing the coronal microleakage of teeth restored with custom cast post and core. In this in vitro original study, after removing 9 mm of gutta percha from root canals of 32 single-canaled premolars, the acrylic resin patterns of the post and core were fabricated. Patterns were cast with Ni-Cr metal alloy and then cemented with glass ionomer. Using self-curing acrylic resin, provisional restorations were made. Then, teeth were randomly allocated to four groups for each temporary cement including zinc oxide eugenol (Temp Bond), zinc oxide noneugenol (Temp Bond NE), noneugenol resin-based temporary luting agent (Temp Bond Clear), and noneugenol urethane methacrylate polymer-based temporary luting agent (Dento temp). Teeth were subjected to thermocycling, immersed in silver nitrate for 6 h and then sectioned. The amount of dye penetration in the margin of provisional crown and into the coronal part of the root canals was measured using a stereomicroscope. Data were analyzed using the Kruskal-Wallis and Dunn tests with significance level of 0.05. All the luting agents showed some degree of microleakage (Group 1 showed the most microleakage). According to the pair comparison between the groups, Group 1 revealed a significant difference with others (P < 0.05). Furthermore, Group 2 showed more microleakage as compared to Group 4 (P = 0.037). Temp Bond showed the highest microleakage as compared to other luting agents. Furthermore, Temp Bond NE revealed a weaker sealability as compared to Dento temp.

Management of Open Apex - A Case Report

Introduction: Endodontic management of immature permanent teeth is a challenge for both clinicians and researchers. Open apex is an unusual wide apical foramen that results due to multiple reasons such as incomplete development, extensive apical resorption, caries leading to pulpal necrosis or trauma before complete root formation, root-end resection, or due to over instrumentation. Aims: To prevent the risk of subsequent microbial contamination. Case Report: This article is a case report showing the management of open apex with MTA plug and a thermoplasticized Gutta Percha technique for obturation. Discussion: Traditionally, calcium hydroxide was used for inducing the formation of an apical barrier. Conclusion: Currently, biomimetic materials like mineral trioxide aggregate (MTA) and biodentin have shown promising results for apexification procedures.

A Comparative Study of the Efficacy of WaveOne and NeoEndo Retreatment File System for the Removal of Gutta Percha from the Root Canal.

Background:Teeth with apical periodontitis that either persisted or developed after initial root canal treatment is most often indicated for retreatment procedure. The present study was conducted to compare the WaveOne and NeoEndo retreatment file system for the removal of gutta-percha from the root canal.Materials and Methods:Fifty extracted mandibular first premolars with single straight roots were divided into two groups, Group I-WaveOne and Group II-NeoEndo. All samples were scanned with cone-beam computed tomography after the root filling and retreatment procedures. The decrease in the volume of filling material after each retreatment protocol was measured.Results:The mean volume of gutta-percha in Group I before retreatment was 5.6 and in Group II was 5.3, after retreatment in Group I was 2.7, and in Group II was 4.2. The mean percentage reduction in Group I was 45.2% and in Group II was 23.8%. The difference was significant (P < 0.05).Conclusion:Both systems failed to completely remove gutta-percha. WaveOne was significantly more effective than the NeoEndo retreatment system.

Non-Surgical Endodontic Retreatment of Anterior Tooth with a Large Periapical Lesion and Extruded Guta Percha with 36 Months Follow-Up: A Case Report

Root canal treatment failure depends on many factors. Overfillings, insufficient fillings, missing canals, failure to provide a complete apical plug, and impermeability of coronal restoration are some of them. Failed root canal treatment may not always manifest itself immediately after treatment. Sometimes, root canal treatments, which have not been done well, can manifest themselves with extensive lesions and severe pain in the apical after a long time. Apical resection may be considered as a solution in lesions that are too large to be treated, but retreatment without surgery should be attempted beforehand. Thus, the patient may have recovered from unnecessary surgical procedures and treated with a more conservative method. This case presents the non-surgical retreatment of a left lateral tooth with a large periapical lesion with extruded gutta percha followed by a 36-month follow-up.

Discovery and rediscovery of gutta percha, a natural thermoplastic

Discovery and rediscovery of gutta percha, a natural thermoplastic

The effect of different sealer volume on bond strength of total fills BC obturating system

The bond strength of Total fill BC obturation system using single cone size 40 and size 50 at different time intervals; 1days and 7 days was evaluated in this vitro study. Twenty four single rooted single canal extracted human teeth were used in this study. All the teeth were instrumented with a crown down technique by Endo Star taper 4% to achieve size #50 apical preparation .irrigation was done using 10ml 2.25% Na(ocl), then 10ml of 17% EDTA and final rinse with 10ml sterile normal saline. Single cone technique was used for obturating all the groups as the manufacturer’s instructions .The samples were classified into two main groups according to the size of the obturating material (n=12); group “I” canals were obturated with Totalfill points size 50 and group “II” (canals were obturated with Totalfill points size 40. Each group was subdivided into 2 subgroups according to the time of the test (n=6); subgroup “1’ examined after 1 day and Subgroup “2” examined after 7 days. Each subgroup is tested using universal testing machine to evaluate the push out Bond strength. after 1 day; there was no statistically significant difference between the two groups, However After 7 days regardless of root level, the Group obturated with size 50 showed statistically significantly higher median push-out bond strength than Group obturated with size 40.it was concluded that The bond strength is higher with the large size of gutta percha core than the volume of sealer.

Comparative Assessment of Canal Transportation, Dentin Loss, and Remaining Root Filling Material by Different Retreatment Files - An In vitro Cross-Sectional Study

Aim:The purpose of this study was to evaluate the centering ability, canal transportation and efficacy of re-treatment rotary and hand files in removing Gutta Percha from root canals using Cone Beam Computed Tomography(CBCT).Materials and Methods:Sixty extracted human maxillary anteriors were obturated and randomly divided into three groups. Root fillings were removed with ProTaper Universal retreatment system, R-Endo system, and Hedstrom files. CBCT scans were taken.Statistical Analysis and Results:The data were analyzed using the Statistical Package for the Social Sciences (SPSS 15.0, IBM). The mean and standard deviation among the groups was calculated by one-way analysis of variance, Kruskal–Wallis, and Mann– Whitney U-tests, and the comparison among the various groups was done by post hoc Tukey's test. A statistically significant amount of remaining root canal filling material and canal transportation was noted (P < 0.05).Conclusion:No system completely removed the root filling material from root canals. Manual instrumentation resulted in more dentin loss and canal transportation than rotary file system.

Evaluation of Fracture Resistance of Mandibular Premolar Canals Filled with Four Different Obturation Techniques- An In vitro Study

Introduction: To prevent the ingress of microorganisms and their byproducts into root canal space is the primary goal of obturation. Endodontically treated teeth are weak and more susceptible to fracture than vital teeth. Therefore, to increase the strength of the root canal and increase root fracture resistance by adhesion and mechanically interlocking root canal filling material with radicular dentin is also one of the goal of obturation. Aim: To evaluate and compare the fracture resistance of mandibular premolar canals filled with four different obturation techniques. Materials and Methods: The in vitro study was conducted in Department of Conservative Dentistry and Endodontics, Krishna Institute of Medical Sciences (Deemed To Be University), Karad, Maharshtra, India, from August 2020 to December 2020. A total of 100 extracted human mandibular premolars with single canals were collected for the study and sectioned horizontally to obtain a standardised length of 14 mm. With the help of 15 K-file (Dentsply) working length was determined and root canals were prepared to an International Organisation for Standardisation (ISO) size 45 file at the apex and flared using a #4 Gates-Glidden drill (Mani, Japan). The teeth were then randomly divided into four experimental groups, Group I-Lateral condensation, Group II-ROEKO GuttaFlow® bioseal, Group III- Guttacore, Group IV-Thermoplasticised gutta percha (System B) and two control groups, they were Group V-(Negative control), instrumentation was done, but no obturation, Group VI-(Positive control), teeth were neither instrumented nor obturated. All the experimental teeth were filled as per the obturation technique. A universal testing machine was used for evaluating fracture resistance. The results were analysed using the One-way ANOVA test. The significance between the groups was tested with Scheffe’s post hoc test. Results: There were statistically significant difference among six group for mean fracture load (N) with p-value &lt;0.001. furthermore, pair wise comparison of fracture load (N) showed that the mean difference is significant at p-value &lt;0.05. The mean difference between Group IV and V was not significant (p-value=0.935) Conclusion: Under the limitation of this study, it was concluded that the resistance of the root to vertical fracture amongst the experimental group was maximum in Group III (Guttacore) and minimum in Group IV (Thermoplasticised gutta-percha, System B).