Gutta-percha Cones Research Articles

Influence of different disinfection protocols on gutta-percha cones surface roughness assessed by two different methods

The objectives of this study were to evaluate how different disinfection protocols affect the surface roughness of gutta-percha (GP) cones used for the dental root canal filling using DIN 4768 standard and another alternative process for assessing roughness of small surface area (multiple profile), comparing both methods in order to identify similarities. The GP cones used were the conventional (C) and a new one impregnated with zirconia oxide, known as the coated cone (CC). Samples were distributed for each group and they were immersed in the correspondent chemical solution as follows: Group 1 (G1), sodium hypochlorite (NaOCl) at 5.25% for 1min; Group 2 (G2), sodium hypochlorite at 2.5% for 10min; and Group 3 (G3), chlorhexidine gluconate (CHX) at 2% for 5min, as recommended by dentistry protocols. The averages and standard deviations of the surface roughness parameters—average roughness (Ra) and root mean square deviation roughness (Rq)—were calculated. Statistical analysis was made before and after immersion by paired t-test. Results showed a statistically significant difference for C GP cones after immersion in 2% CHX and 2.5% NaOCl (p<0.01). No difference was found in CC GP cones. DIN 4768 standard and multiple profile measurements showed similar trends and behavior.

Accuracy of two different voxel sizes for presurgical evaluation of mandibular osteotomy

The aim of this study was to compare the accuracy of two different voxel resolutions for the preoperative assessment of mandibular osteotomies. The study was conducted on 37 dry adult human mandibles. To obtain measurement standardization, heated gutta-percha cones were placed on the dry mandibles to mark 20 anatomical points. These cones were used for all measurement groups. Cone beam computerized tomography (CBCT) scans of the mandibles were made using 0.200mm3 and 0.400mm3 voxel sizes (Planmeca Promex-3D Helsinki, Finland). The results obtained from these two groups were compared with physical measurements obtained using a digital calliper, in order to analyze their predictive value. In the study, one voxel size did not have supremacy over the other in terms of accuracy. For mandibular osteotomies, 0.400mm3 voxel size can be preferred because of lower radiation dose.

Sealability of Different Root Canal Nanosealers: Nano Calcium Hydroxide and Nano Bioactive Glass

Background: The success of the endodontic treatment is largely dependent on the sealing achieved by root canal obturation. The application of sealer fills imperfections and increases adaptation of the root filling to the canal walls. Aim: To evaluate the sealability of experimental nanosealers (nano calcium hydroxide and nano bioactive glass) and to compare it with the commercial zinc oxide eugenol sealer using a dye penetration method. Materials and Methods: Sixty single-rooted mandibular premolars were selected. The tooth crowns were removed so as to obtain standardized 15-mm-long root specimens. The root canal was instrumented with Protaper Ni-Ti rotary file and the final file size was up to # F4/.06 (in vitro study). They were then randomly allocated into 3 groups of 20 specimens each (n=20) according to the sealer used for obturation, and all samples were filled with single cone gutta-percha (#40/06) and one of the tested sealers. All teeth were coated with nail polish and then suspended in 2% methylene blue dye for 7 days. Stereo-microscope (x10) was used to evaluate the sealability of newly introduced nanosealers. The data were statistically analyzed by ANOVA test followed by post hoc analysis (P &lt; 0.05). Results: Significant improvement shown by the presented study suggests that nano calcium hydroxide sealer showed significantly less dye leakage than nano bioactive glass sealer and zinc oxide eugenol sealer. Conclusion: The results of this study showed that the synthesized nano-powder sealers are suitable for use in root canal therapy to prevent leakage. The root canal can be sealed better by using smaller nano-powder particle sizes. In addition, the two groups exhibited significant differences in leakage in comparison with commonly used ZOE sealer.

Ultrasound Examination to Visualize and Trace Sinus Tracts of Endodontic Origin

IntroductionThe detection of a tooth with a sinus tract (ST) of endodontic origin and its pathway are conventionally assessed with a periapical radiograph and a gutta-percha cone introduced into its stoma. The aim of this study was to evaluate the possibility to detect STs and trace their route using ultrasound real-time examination. MethodsTwo calibrated examiners performed echography on 10 patients who had a lesion of apical periodontitis (AP) and ST and 10 patients in the control group with AP without an ST recruited in 2 endodontic practices. They also traced the pattern of the STs with a computer program. The images were then submitted to 2 calibrated and blinded observers who were asked to describe the presence of AP and ST and to trace it with the same program. The data obtained were compared with the clinical and radiographic diagnosis of ST. For sensitivity, specificity, accuracy, and positive and negative predictive values, the receiver operating characteristic curve and Fisher exact test were used (P < .05). ResultsInterobserver agreement was high as was the diagnostic accuracy of the ultrasound examination of STs (mean value = 97.5%), and the Fisher exact test showed statistical significance (P < .05). High sensitivity and a negative predictive value and 100% specificity and a positive predictive value were also obtained. The application of the 3-dimensional mode further enabled the reconstructions of the more complex paths, and the implementation with color power Doppler disclosed the vascularity surrounding the STs. ConclusionsThe ultrasound examination is a technique feasible to describe and trace the STs of endodontic origin.

The single cone obturation technique with a modified warm filler

ObjectivesThe aim of this study was to develop a new method of obturation by warm sealer in conjunction to single cone gutta-percha and evaluate the suitability of this technique to obturate complex root canal systems. MethodologyThree root canal sealers namely, AH Plus, BioRoot RCS, GuttaFlow and a prototype sealer composed of tricalcium silicate and 30% zirconium oxide mixed with water and water-soluble polymer were investigated. The sealers were tested for flow, film thickness, setting time and radiopacity following ISO 6876 (2012) recommendations at room temperature and following heat application at 100°C to change the sealer properties. All the test sealers were characterized by scanning electron microscopy and energy dispersive spectroscopy. The volume of voids when used with a single cone obturation technique both unmodified and modified by heat was evaluated using microcomputed tomography. ResultsAlthough the prototype sealer was designed to be similar to the BioRoot, its physical properties were found to be different. All sealers tested were affected by the heat and exhibited a change in the physical properties mainly the setting time, flow, film thickness and void volume. ConclusionsThe application of heat affected the sealer properties and void volume. The single cone obturation technique may not be suitable for complex canal anatomy and furthermore, AH Plus should not be subjected to high temperatures as its properties deteriorate and void volume increases.

Tannik asitle irrigasyonun kök kanal dentininin mikrosertliğine ve epoksi rezin esaslı patın bağlanma dayanımına etkisi

Background:To investigate the effects of final irrigation protocols with tannic acid (TA) on dentin microhardness and push-out bond strength of an epoxy resin based sealer to root canal dentin.Methods: The 50 root halves were embedded in an acrylic resin for the microhardness measurement. After the initial baseline microhardness measurement, the root halves were randomly divided into 5 groups according to the final irrigation protocol used (n=10): distilled water (control), 10% TA for 2 min, 10% TA for 5 min, 20% TA for 2 min, and 20% TA for 5 min. After surface treatment, dentin microhardness values were recorded at close proximity to the initial indentation areas. For push-out bond strength test the root canals of 75 single-rooted mandibular premolars were instrumented and the final irrigation protocols were applied as described above (n = 15). Following root canal obturation with single cone technique using matched-taper gutta percha cones and an AH Plus- epoxy resin based root canal sealer, 1 mm-thick slices were obtained from the middle third of the root canals. Push-out bond strength test was applied. The data were analyzed using One way ANOVA and Tukey HSD tests (p=0.05).Results:In TA-treated groups, there was a significant increase in the microhardness values compared with the control group (p&amp;lt;0.05). Regarding the push-out bond strength test, there was no significant difference between the bond strength values of 10% TA for 2 min and the control group (p&amp;gt;0.05). The remaining groups presented higher bond strength values than the control group (p&amp;lt;0.05).Conclusions: TA increased dentin microhardness and the bond strength of the epoxy resin based sealer to root canal dentin. The effect on the bond strength was time and concentration dependent. Keywords: bond strength, dentin, microhardness, epoxy resin based sealer, tannic acid

SINGLE VISIT ENDODONTIC TREATMANT ON LEFT MAXILLARY FIRST MOLAR WITH RECIPROCAL SYSTEM

Root canal treatment is a step in order to maintain and preserve the tooth. The success rate of root canal treatment depend on the degree of severity illness and treatment techniques are performed. Reciprocal system is a techniques which use rotary one file endodontic. Single visit endodontic on this treatment was chosenbecause this techniques provide distinct advantages, more economical cost and risk contamination can be reduced.Female 25 years-old with irreversible pulpitis in maxillary left first molar, on the X-ray picture of the root canal looks normal without any narrowing. Root canal treatment performed with anesthetic infiltration pehacain 2 %, using rubber dam during treatment to prevent bacterial contamination and access opening. Tooth preparation using rotary one file reciprocal system R#25, previously done by determining work length with apex locator and confirm with X-ray picture. Obturation with single cone gutta percha R#25 and top seal paste, cotton pellet and temporary material filling. Control after 24 hours, percussion is negative, patient feel little ofpain.Success of endodontic is depend on eliminate bacterial product. One visit endodontic with reciprocal technique is a relatively reliable and simple technique for dentist.

Evaluation of the Effect of Irrigating Solutions on Push out Bond Strength of Bio-ceramic and Resin Based Endodontic Sealers

Purpose: This study was designed to assess the impact of irrigating solutions on push out bond strength of Bio- ceramic and Resin Based Endodontic Sealers. Materials and Methods: sixty extracted human teeth with straight single root and canal were prepared with RACE FKG rotary files till size 35 taper 06. The samples were dispensed into two experimental groups according to root canal sealer used (30 samples each). group1 Total Fill Bio-ceramic sealer after irrigation with, and Group 2: AH Plus sealer. The specimens were further randomly divided into two sub groups (15 samples each) according to the final irrigation solution applied. Group (a): irrigated with 17%EDTA, Group (b): irrigated with 2% CHX. The samples were obturated by single cone gutta-percha with vertical condensation technique. Results: regarding the groups. The type of irrigating solution affects the bond strength of total fill bio ceramic sealer. Final irrigation with EDTA result the highest bond strength of bio ceramic sealer to root dentine which was scientifically different from irrigation with CHX. Bond strength values was as follows: EDTA>CHX. Conclusion: the type of final irrigation affects the adhesion of total fill bio ceramic and AH plus sealers to root canal dentine

Influence of Two Root Canal Obturation Techniques with Resin Based Sealer to Enterococcus faecalis Penetration

&#x0D; Introduction: Root canal treatment is done to maintain the teeth to last as long as possible in the oral cavity. This can be done with three main stages, such as biomechanical preparation, sterilization and root canal filling. The hermetic seal of root canal filling has an important role in the success of root canal treatment.&#x0D; &#x0D; Objective: The aim of this in-vitro study is to compare the microbial leakage of root canals filled with RealSeal sealer/Resilon Points and AH Plus sealer/conventional gutta-percha points using single cone obturation technique and warm vertical compaction technique.&#x0D; &#x0D; Methods: Thirty-two extracted human mandibular premolars with single canals were decoronated to a standardized root length of 15 mm and prepared using crown-down technique to a master apical file size 30/.09. Teeth were divided into four experimental groups (n = 7 each group) and controls (n = 1 each group). Teeth in the first group were obturated using single master gutta percha cone and AH Plus sealer and second group were obturated with single Resilon gutta percha cone using RealSeal sealer. Third experimental group was filled with warm vertical condensed gutta-percha using AH Plus sealer and fourth group was filled with warm vertical condensed Resilon gutta-percha using RealSeal sealer. The coronal chamber of each sample were inoculated with Enterococcus faecalis.&#x0D; &#x0D; Results: There were no significant differences between the four experimental groups (P = 0.182). The differences occurred only in the survival time from each group. Group 4 (Warm Vertical Condensation - RealSeal) has the lowest leakage rate among other groups and the fastest leakage occurred in group 1 (Single Cone - AH Plus).&#x0D; &#x0D; Conclusion: The single-cone techniques does not insure durable apical seal against bacterial leakage. Warm vertical compaction technique using Resilon gutta-percha and RealSeal sealer appears to be more effective in minimizing bacterial leakage than gutta-percha and AH Plus sealer.

Influence of Two Root Canal Obturation Techniques with Resin Based Sealer to Enterococcus faecalis Penetration

Introduction: Root canal treatment is done to maintain the teeth to last as long as possible in the oral cavity. This can be done with three main stages, such as biomechanical preparation, sterilization and root canal filling. The hermetic seal of root canal filling has an important role in the success of root canal treatment. Objective: The aim of this in-vitro study is to compare the microbial leakage of root canals filled with RealSeal sealer/Resilon Points and AH Plus sealer/conventional gutta-percha points using single cone obturation technique and warm vertical compaction technique. Methods: Thirty-two extracted human mandibular premolars with single canals were decoronated to a standardized root length of 15 mm and prepared using crown-down technique to a master apical file size 30/.09. Teeth were divided into four experimental groups (n = 7 each group) and controls (n = 1 each group). Teeth in the first group were obturated using single master gutta percha cone and AH Plus sealer and second group were obturated with single Resilon gutta percha cone using RealSeal sealer. Third experimental group was filled with warm vertical condensed gutta-percha using AH Plus sealer and fourth group was filled with warm vertical condensed Resilon gutta-percha using RealSeal sealer. The coronal chamber of each sample were inoculated with Enterococcus faecalis. Results: There were no significant differences between the four experimental groups (P = 0.182). The differences occurred only in the survival time from each group. Group 4 (Warm Vertical Condensation - RealSeal) has the lowest leakage rate among other groups and the fastest leakage occurred in group 1 (Single Cone - AH Plus). Conclusion: The single-cone techniques does not insure durable apical seal against bacterial leakage. Warm vertical compaction technique using Resilon gutta-percha and RealSeal sealer appears to be more effective in minimizing bacterial leakage than gutta-percha and AH Plus sealer.

Management of Traumatic Inflammatory External Root Resorption

Inflammatory external root resorption is one of the major complications after traumatic dental injury. It is characterized by the loss of mineralized dental tissue which may, ultimately, result in loss of the tooth. However, with appropriate treatment, prognosis for these teeth may be greatly improved, by preventing or arresting resorption. This is a case report of an 18-year-old male patient presented with root canal treated maxillary central incisors associated with pain; with history of trauma. Radiographic examination revealed periradicular lesion and associated pathologic resorption of the apical region of the root with respect to 11 and calcification of the root canal with inadequate obturation with respect to 21. After removal of the root canal filling, 11 was disinfected with intracanal double antibiotic paste. After 2 weeks, antibiotic dressing was removed, and apical third was sealed with mineral trioxide aggregate (MTA). Canal was obturated with custom-fit gutta-percha and accessory cones with AH plus sealer and restoration was done with composite resin. Retreatment was also done with respect to 21 and obturated with custom-fit gutta-percha. Both teeth were restored with E-max crown. No clinical symptoms were apparent, significant osseous healing of the periradicular region was observed with arrest of external root resorption with respect to 11 and no periapical changes were apparent with respect to 21 on subsequent follow-up.

Effectiveness of three chemical solutions on gutta-percha cones by rapid sterilization technique: A scanning electron microscope study

Objectives: It is prudent to produce most sterile root canal system and to maximize the most hermetical seal so gutta percha cones are used gutta-percha (GP) cones are used to obliterate the root canal space, prevention of contamination becomes a problem. Hence, the aim of this study was to identify the presence of surface topography, crystallization, and subsequent removal of sodium hypochlorite, glutaraldehyde, and aloe vera crystals on GP cones after rapid sterilization technique – this was a scanning electron microscope (SEM) study. Materials and Methods: GP points of size 80 and 2% taper were divided into four groups depending on the type of agents used for sterilization. Four groups were as follows: Group 1 – control group, Group 2 – aloe vera, Group 3 – glutaraldehyde, and Group 4 – NaOCL. About 10 GP points of each group were immersed for 2 min in different concentrations and observed under SEM to evaluate surface topography and presence of chloride crystals. Statistical analysis was carried out using Chi-square test, Kruskal–Wallis test and Mann–Whitney U-test to compare the results between the groups. All the data were expressed as mean ± standard deviation, and the difference of P Results: Aloe vera found to be an effective medium in decontaminating GP cones compared to glutaraldehyde and NaOCL (sodium hypochlorite). Conclusion: The use of sterile GP cones for the obliteration of root canals is of prime importance, and aloe vera group shows no changes in surface topography and the presence of chloride crystal formation compared to the control group. Hence, it is prudent to produce the most sterile root canal system, maximizing most hermetical seal possible.

Comparative evaluation of silver nanoparticles and 5.25% sodium hypochlorite for rapid chairside decontamination of artificially infected gutta-percha with Escherichia coli: An In vitro Study

Introduction: The aim of this in vitro study was to evaluate the efficacy of 50 ug/ml silver nanoparticle (AgNPs), 70 ug/ml AgNPs and 5.25% sodium hypochlorite (NaOCl) as a final irrigant for rapid chair-side decontamination of artificially infected gutta-percha (GP) with Escherichia coli. Materials and Methods: A series 120 GP cones size 40 were taken from new batch. The GP cones were divided into six experimental group: Group I - Evaluation of the contamination of GP cones in manufacturer's Box-(20 GP cones), Group II - Evaluation of GP artificially contaminated with E. coli(100 GP cones), Group III - Evaluation of the effectiveness of 50 ug/ml of AgNPs for decontamination of the cones contaminated with E. coli(n = 20 GP infected cones from Group II), Group IV - Evaluation of the effectiveness of 70 ug/ml of AgNPs for decontamination of the cones contaminated with E. coli(n = 20 GP infected cones from Group II), Group V - Evaluation of the effectiveness of 5.25% NaOCl for decontamination of the cones contaminated with E. coli(n = 20 GP cones infected from Group II). Aliquots from the experimental GP cones were plated on brain heart infusion agar (HiMedia Lab, Mumbai, India), and the colony-forming units were evaluated under colonimeter. Results: 70 ug/ml of AgNPs, 5.25% NaOCl exhibited similar antimicrobial effect (P = 1). Highly significant difference were found when 50 ug/ml compared with 70 ug/ml and 5.25% NaOCl (P < 0.0001). Conclusion: 70 ug/ml of AgNPs (0.007%) proved to be highly effective against E. coli and showed similar antimicrobial efficacy as 5.25% NaOCl at 750 times lesser concentration.

Evaluation of Apical Dimension, Canal Taper and Maintenance of Root Canal Morphology Using XP-endo Shaper

To evaluate the shaping ability of the XP-endo shaper file system in maxillary molars, representing root canals with variation in morphology. Twenty maxillary molars were instrumented according to recommended protocols. Pre- and postoperative microcomputed tomography (CT) scans were performed and the root canals classified according to canal type and curvature. The volume change, number of strokes needed to prepare the canals and the size of the 0.04-tapered gutta-percha cone that was adapted in the canal were recorded. XP-endo shaper created a significant change in volume after instrumentation in all the canals, with the biggest change found in the DB canals, followed by the MB1 and the P canals, both at full length and in the apical 4 mm. The number of strokes needed to achieve working length and final shape did not differ between the various root canals. Although not significant, the number of strokes needed to prepare the root canal increased with severity of the curvature, but the severity of the curvature did not result in increased removal of dentin in the apical 4 mm. It was possible to achieve a final root canal size where a 0.04-tapered gutta-percha cone could be adapted. The XP-endo shaper was a safe and effective instrument to achieve a root canal preparation of at least size 30 and a 0.04 taper. The clinical performance of XP-endo shaper was to some extent dependent on preoperative volume and curvature of the root canal.

A case report analysis of the radix entomolaris of 12 years old patient

Endodontic treatment requires a thorough knowledge of the anatomy of the teeth and their surrounding structures. Root canals may be left without treatment during endodontic therapy if the dentist doesn't know the anatomic variations for each tooth. Usually, the mandibular first molars would have two roots with three canals (mesiobuccal, distal and mesiolingual) but in few of the teeth, the number of canals, and the roots will vary. An additional lingual root otherwise referred to as a mandibular first molar Radix Entomolaris and an extra Buccal root, also called a Radix Paramolaris with two distal roots is an exciting example of anatomic variation. This paper expounds upon the case report of a mandibular first molar with three roots and four canals [Radix Entomolaris (RE)]. A 12-year-old male patient, was diagnosed with Radix entomolaris on radiographic examination and treated in the Department of Conservative Dentistry and Endodontics. Mesiobuccal and mesiolingual and the radix canals were enlarged up to F2 ProTaper files. As the distal root was curved, it was enlarged only up to F2 ProTaper file. The root canals were medicated with a calcium hydroxide dressing, and the access cavity was temporarily sealed with a temporary restoration. One week later the root canals were obturated. Initially, the corresponding sized gutta-percha master cones for the four canals were selected. The selected gutta-percha cone was coated with AH Plus sealer and placed in the canal. The System B Heat Source was adopted to remove the coronal portion of the gutta-percha cone. The Obtura II system was used for the back-filling with the application of gutta-percha in small increments. The access cavity was sealed with miracle mix (silver alloy admix GIC), and the root canal filling was evaluated radiographically.

Assessment of the Ability of Different Cleaning Protocols to Remove Eugenol-based Endodontic Sealer from the Root Dentin

The aim of this study is to assess three protocols for removing a eugenol-based filling endodontic sealer from root dentin and the bond strength of fiberglass posts luted with resin cement. Sixty single-rooted bovine teeth were prepared and filled with the Endofill endodontic sealer and gutta-percha cones. After 7 days, 9 mm of the filling was removed and divided into five groups (n = 12) according to the cleaning protocol: G1 (negative control), unfilled; G2 (positive control), saline solution; G3, 95% alcohol; G4, amyl acetate; and G5, Largo bur. The canals were hybridized and fiberglass posts were luted with resin cement. They were cross-sectioned in slices of 1 mm and subjected to the push-out test. The other samples were sectioned longitudinally and visualized in a scanning electron microscope (SEM), and the existing chemical elements were quantified by energy dispersive spectroscopy (EDS). The statistical analysis used variance analysis (ANOVA)-one way and Fisher's test, at a 5% significance level. The 95% alcohol was more effective for cleaning the canal, resulting in a clean dentinal surface and bond strength statistically similar to the negative control. The Largo bur was also statistically similar to the negative control, with only a few debris impregnated on the wall. Amyl acetate showed more sealer residues on the canal walls, with a consequent lower bond strength value than the other groups, only higher than the positive control. The 95% alcohol and Largo burs may be used after removing the canal filling, so that the bond strength is improved when using the eugenol-based sealer.

An in vitro evaluation of antimicrobial efficacy of 5% sodium hypochlorite, 2% chlorhexidine, and herbal extracts of neem and Aloe vera in disinfection of gutta-percha cones

Aim: An in vitro evaluation of the antimicrobial efficacy of 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and herbal extracts of neem and Aloe vera in disinfection of gutta-percha cones for 3 min. Materials and Methods: Ninety gutta-percha cones were used for the study. They were divided into four groups (Group A, Group B, Group C, and Group D) based on the decontaminant used. Each group had 20 cones which were further divided into two subgroups. The microbial species used were Enterococcus faecalis and Staphylococcus aureus. Ten cones in each subgroup were contaminated with E. faecalis and S. aureus, respectively. From the remaining 10 cones, five were contaminated with E. faecalis and five with S. aureus and were disinfected with normal saline which was used as a control. These concentrated extracts were obtained using Soxhlet extraction procedure and were tested for their antimicrobial efficacy by counting the colony-forming units (CFU). The count was taken on day 0 (immediately after disinfection) and day 7 (after incubation for 7 days) for each organism. Results: The number of CFU on day 0 was not more than five for both E. faecalis and S. aureus for all the disinfectants used. However, on day 7 after incubation, only the neem group showed full growth of colonies for the E. faecalis. Conclusion: Long-term effects of A. vera were found to be superior and comparable to 5% NaOCl and 2% CHX. However, the long-term effect of neem as a disinfectant for gutta-percha needs further investigation.

Comparing the Efficacy of Berberine against Sodium Hypochlorite and Chlorhexidine Cetrimide as a Chairside Disinfectant of Gutta Percha Cones

Comparing the Efficacy of Berberine against Sodium Hypochlorite and Chlorhexidine Cetrimide as a Chairside Disinfectant of Gutta Percha Cones

Comparison of the fitness of matching single gutta-percha cones in canals prepared with Waveone and Waveone Gold systems

Comparison of the fitness of matching single gutta-percha cones in canals prepared with Waveone and Waveone Gold systems

Comparative Evaluation of Depth of Penetration of Root Canal Irrigant After Using Manual, Passive Ultrasonic, and Diode Laser-Assisted Irrigant Activation Technique.

AIM:The aim of this study was to compare and evaluate the penetration depth of irrigant after using manual, passive ultrasonic, and diode laser–assisted irrigant activation technique.Materials and Methods:Ninety extracted single-rooted human mandibular premolars were selected (N = 90). Teeth were decoronated and working length was standardized for 12 mm. Root canals were shaped using ProTaper Universal F3. Saline was used as an irrigant in between every successful instrumentation. After completion of chemomechanical preparation, root canals were filled with Rhodamine-B-labeled sodium hypochlorite (3%). Teeth samples were divided into three experimental groups. In Group 1 (n = 30), manual irrigant activation was performed for 30 seconds with master cone gutta-percha (F3) in gentle up and down motion. In Group 2 (n = 30), ultrasonic irrigant activation was performed using passive ultrasonic endo tips for 30 seconds. In Group 3 (n = 30), laser activation was performed using diode laser for 30 seconds with 200-µm tips. Transverse sections were made at 2, 5, and 8 mm from the root apex and were observed under confocal laser scanning microscopy. Maximum penetration depth of irrigant was recorded and statistically analyzed.Result:In the all three groups, coronal third (sections at 8 mm from root apex) showed the highest penetration depth of irrigant. Laser activation (Group 3) showed the highest penetration depth in all the three sections when compared with manual and passive ultrasonic irrigant activation. One-way analysis of variance and post hoc test showed there were significantly high differences among all the three groups and also at all three levels (P < 0.05).Conclusion:Diode laser–assisted irrigant activation technique had better penetration depth in all the three aspects of root dentin.