This research is carried out within two caves with underground river and one karst spring, which is located in Gunung Sewu karst area, Gunung Kidul. The objective of this research is to recognize some flood hydrograph properties of karst aquifer characterization in order to compare the comportment of aquifer to release its flow components between underground river and karst spring. Water level data loggers are installed within two caves and one karst spring represent upper, middle, and lower elevation of karst topography, which are Beton, Seropan, and Toto Cave, respectively. In addition, time series discharge measurement in correspond to minimum, average, and maximum flow events is conducted to formulate the Annual Stage Discharge Rating Curve. Thereby, the full year river hydrograph may be defined. Afterwards, by selecting several flood hydrograph events, the recession constant in each cave including diffuse flow (Kb), and fissure flow (Ki), and conduit flow (Kc) recession constant, is then calculated and compared in every location. The result shows that Beton Spring that represents the upper part of research area confirms the quickest respond towards rainfall events. Also, this point specifies the medium number of diffuse recession constant (Kb=0.983), compared to Seropan (0.996), and Toto (0.937), means that the aquifer surrounding Toto Cave release its groundwater storage faster than Beton Spring and Seropan Cave. On the contrary, Seropan Cave performs the highest value of diffuse recession constant (0.996) and this situation is confirmed by its dry season discharge that still above 810 lt/second.