Coastal Waters Of Gulf Research Articles

Genomic diversity of Vibrio spp. and metagenomic analysis of pathogens in Florida Gulf coastal waters following Hurricane Ian.

Evidence suggests warming temperatures are associated with the spread of potentially pathogenic Vibrio spp. and the emergence of human disease globally. Following Hurricane Ian, the State of Florida reported a sharp increase in the number of reported Vibrio spp. infections and deaths. Hence, monitoring of pathogens, including vibrios, and environmental parameters influencing their occurrence is critical to public health. Here, DNA sequencing was used to investigate the genomic diversity of Vibrio parahaemolyticus and Vibrio vulnificus, both potential human pathogens, in Florida coastal waters post Hurricane Ian, in October 2022. Additionally, the microbial community of water samples was profiled to detect the presence of Vibrio spp. and other microorganisms (bacteria, fungi, protists, and viruses) present in the samples. Long-term environmental data analysis showed changes in environmental parameters during and after Ian were optimal for the growth of Vibrio spp. and related pathogens. Collectively, results will be used to develop predictive risk models during climate change.

Results of monitoring of microalgae of harmful algal bloom (HAB) complex in the Avachisky Gulf in 2022

The object of the research is microalgae of HAB complex (harmful algal “bloom”). The purpose of the work is to provide monitoring data on HABs and the microalgae habitat conditions near the eastern coast of Kamchatka, which is currently the focus of attention of the Kamchatka government in terms of development of recreational and tourist activities, one of the priority economic directions. Suface water samples were collected at the coastal transect, stretching along the whole coast of the Avachinsky Gulf in the process of the work from April to October. Hydrological data were collected with Rinko- Profi lerASTD-102 and YCI CastAway probes. In June, the data were collected as part of planned ichthyoplankton survey. As a result, a real database on taxonomic composition, quantitative vales and distribution of microalgae of the HAB complex was formed. Data on water temperature and salinity, biogenic background of the Avachinsky Gulf coastal waters were obtained.

Different Responses of Chlorophyll a to the Passage of the Tropical Storm Wipha (2019) in the Coastal Waters of the Northern Beibu Gulf

Tropical storms (TS) are important drivers of short-term changes and affects the coastal and marine environment. Based on in situ observational data from four locations in the coastal area of the northern Beibu Gulf and satellite data, we analyzed the changes in temperature, salinity, and turbidity during the transit of TS “Wipha” in 2019 and assessed the environmental factors controlling chlorophyll a concenteation (Chl-a) increases in the coastal area. Our results showed that in the coastal area, the growth of phytoplankton after the TS was mainly controlled by the nutrient and light availability. The increased input of freshwater by TS, including direct inputs from rainfall and increased river discharge, reduced the salinity. The decrease in salinity may indicate an increased input of nutrient-rich freshwater at all four stations (nutrients input: S1>S2>S3>S4). Nutrient concentration at S1, S2, and S3 implied by salinity was high, but that at S4 was limited. The shorter recovery time of turbidity after TS indicated the faster improvement of light conditions in this area [recovery time: S4 (2 days)< S1 (3 days)< S3 (5 days)< S2 (10 days)]. The high turbidity associated with poor light penetration was an important factor limiting phytoplankton growth at station 2, with a slow recovery of the turbidity to pre-TS levels. The rapid recovery of the turbidity to the pre-TS levels at S1, S3, and S4 suggested good light conditions soon after the TS, and probably led to a significant increase in Chl-a after the TS ([Chl-a]: S1>S3>S4). The less of an increase of Chl-a at S4 was not only related to nutrient restriction but also related to weak mixing of the water column, while the least significant decrease in the SST at S4 implied that the enhancing mixing after TS was limited.

Bioprospecting autochthonous marine microalgae strain from the Arabian Gulf Seawater, Kuwait for biofuel feedstocks

Bioprospecting programmes are the key to increasing the current portfolio of indigenous microalgal strains accessible for different applications in microalgal biotechnology. In this work, nine fastgrowing microalgal strains isolated from the Arabian/Persian Gulf coastal waters of Kuwait were evaluated for their potential as biofuel feedstocks. 18S rRNA gene sequencing showed that the strains belong to five different genera, namely, Chlorella, Nannochloris, Scenedesmus, Tetraselmis, and Nannochloropsis. In terms of the total lipid content, in comparison to the other strains, Tetraselmis sp. KUBS13G and Tetraselmis sp. KUBS16G displayed higher lipid contents of 29.56% dry weight (DW) and 26.13% DW, respectively, which were dominated by palmitic and oleic acids. Fuel properties calculated from the fatty acid methyl esters (FAMEs) by empirical equations were compared with EN14214 (European) and ASTM D6751-−02 (American) biodiesel standards. Multicriteria decision analysis (MCDA) methods, such as the Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE) and Graphical Analysis for Interactive Assistance (GAIA), were used to select suitable microalgae for biofuel feedstock based on their biodiesel fuel properties. Overall, the results suggested that the indigenous microalgal strain Tetraselmis, in particular Tetraselmis sp. KUBS37G, and Scenedesmus sp. KUBS17R are the most suitable strains for biofuel feedstock owing to their improved fuel properties, such as density (ρ) (0.88 g cm−3), low kinematic viscosity (3.1 mm2 s−1), high cetane number (54 and 56, respectively), high oxidation stability (14.6 hr and 14.8 hr), and cold-filter plugging point (1.0°C and -6.1°C).

Interference of CDOM in remote sensing of suspended particulate matter (SPM) based on MODIS in the Persian Gulf and Oman Sea

The spatial and temporal variability of suspended particulate matter (SPM) in the Persian Gulf and Oman Sea coastal waters has remained challenging to understand among researchers. Here, for the first time in the region, we parametrized SPM concentration in the study area utilizing derived remote sensing reflectance (Rrs) values from Moderate-resolution Imaging Spectroradiometer (MODIS), using 555 and 667 nm wavelengths. Likewise, the findings showed that the developed optical model based on the optical ratio of Rrs (667)/Rrs (555) was sensitive to the concentration of Chromophoric dissolved organic matter (CDOM) in the seawater, within the visible wavelengths less than 600 nm. Comparing the new estimates of the SPM concentration with in situ measurements by Spearman's Rank correlation for validation revealed that the association between estimated and measured SPM concentration would be considered statistically significant (ρ up to 0.86, p < 0.05). This study increased the average accuracy of the estimates up to 73%.

Red Tide: Why a Biologic, HDFx, and Magnesium Could be used to allay Inflammatory and Lung Problems Caused by Algal Blooms: Hypothesis

Over the past decade, there has been a growing concern over invasion of Florida Gulf Coastal Waters and beaches by several algal blooms created by “red tides”. These algal blooms create various toxins that pose high risks for marine life, livestock, birds, and humans [1, 2]. The algae become so numerous that they discolor gulf waters and beaches. Since the 1980’s, red tides have been seen in many countries throughout the globe (i.e., South America, Central America, Japan, caribbean, South Pacific regions, etc.).

Biodiversity and Abundance of Plankton Communities along the Coastal Waters of Gulf of Aqaba, Saudi Arabia

Biodiversity and Abundance of Plankton Communities along the Coastal Waters of Gulf of Aqaba, Saudi Arabia

Response of Corals Acropora pharaonis and Porites lutea to Changes in pH and Temperature in the Gulf

Coral reefs are harboring a large part of the marine biodiversity and are important ecosystems for the equilibrium of the oceans. As a consequence of anthropogenic CO2 emission, a drop in pH and an increase in seawater temperature is observed in the Gulf coastal waters that potentially threaten coral assemblages. An experimental study was conducted on two species of corals to assess the effect of ocean warming and ocean acidification on the net calcification rate. Two pH conditions 8.2 and 7.5 and three temperatures, 22.5, 27.5 and 32.5 °C, were considered. Net calcification rates were measured using 45Ca radiotracer. Both temperature and pH had a significant effect on net calcification rates following a similar pattern for both species. The highest calcification rate was observed at low temperature and high pH. Increased temperature and decreased pH led to a decrease in net calcification rates. An interactive effect was observed as the effect of pH decreased with increasing temperature. However, the two species of coral were able to calcify in all the tested combination of temperature and pH suggesting that they are adapted to short term changes in temperature and pH. Ability to calcify even at a high temperature of 32.5 °C that is identical to the summertime Gulf seawater temperature under both the ambient and low pH condition with no mortalities, raises a question: are these corals adapted to high seawater temperatures and low pH? More in-depth assessments will be required to confirm if this is an adaptation to higher temperatures in Persian Gulf corals.

Evaluating suitability of source water for a proposed SWRO plant location

SWRO membranes are characterized by their ability to reject particles of larger than 0.001 μm diameter and approximately 200 molecular weight. This indicates the selectiveness of the SWRO membranes and the relative ease by which they can be clogged or fouled. For this reason, membrane filtration needs the cleanest possible feed water. Without extensive feed water purification, the reverse osmosis (RO) technology of simple filtration under pressure could be fraught with problems. The quality of source water determines the pretreatment regimen and consequently success or failure of a SWRO plant. The present study evaluated the suitability of source water for a proposed SWRO plant on the Gulf coast of Saudi Arabia.Various physico-chemical and biological parameters were assessed: temperature, pH, salinity, conductivity, total dissolved solids (TDS), silt density index (SDI), turbidity, total suspended solids (TSS), total hardness, total alkalinity, total organic carbon (TOC), dissolved carbohydrates and proteins, dissolved oxygen (DO), biochemical oxygen demand (BOD), chlorophyll-a, bacterial count, major ions, and trace metals. With the exception of total suspended solids, chemical and physicochemical variables measured in this study had concentrations typical of the Arabian Gulf water and seawater in general. Average TSS values were slightly higher than those reported for Gulf coastal waters, and more importantly they were highly variable. This variability may result in episodes of filtration problems for the SWRO plant. The plant should seek deeper sea water a distance from shore as the location of its intake. The study allowed recommendations for treatment options to assure more successful operation of the plant.

Growth, mortality and exploitation of buccaneer anchovy (Encrasicholina punctifer Fowler, 1938) from the north Persian Gulf and Oman Sea

The population parameters of Encrasicholina punctifer (Buccaneer anchovy) from commercial fishery in the Persian Gulf and Oman Sea coastal waters were studied from October 2014 to September 2015. A total of 13951 length-frequency data collected monthly by pair-boat purse seine and beach seine methods. The asymptotic length (L∞) and Von Bertalanffy growth function (K) were 11.5 cm and K = 1.4 per year, respectively. The value of to was calculated as -0.14 year. The longevity estimated 2 years. The total length ranged from 2.9 to 10.1 cm, with average 6.5 cm. The probability of capture were calculated in total length as L25=6.05 cm, L50=6.48 cm and L75=6.93 cm. Four cohorts distinguished annually with mean length of 4.56, 5.77, 7.21 and 8.50 cm. The length-weight relationship was determined as W= 0.0051 L 3.1114. Total, natural and fishing mortality parameters were estimated, 6.15, 2.84 and 3.32 yr-1 respectively. The current rate of exploitation (E) was given as 0.54, the values of exploitation ratio was found reasonable for current fishing effort. From the yield-per-recruit analysis, Emax, was estimated as 0.91. The results indicated that the Buccaneer anchovy stock was not overexploited, since E<Emax, it confirmed the good potential of anchovy stock in the area.

Environmental Studies on the Aqaba Gulf Coastal Waters during 2011-2013

In order to assess the quality of the Aqaba Gulf coastal waters for the sustainable use and development, and consequently for the national income, four field campaigns were annually carried out during the period from 2011-2013 to monitor hydrographic variables, i.e. temperature, salinity, pH, etc., nutrients, some heavy metals, i.e. iron, lead, copper, cadmium, chromium, etc. in addition to petroleum hydrocarbons. Except for the area of Sharm El-Sheikh Harbor, the results for the density, composition, and distributions of beach litter cleared out that the Egyptian shoreline of the Aqaba Gulf is relatively not affected by man-made litter. The area of Sharm El-Sheikh Harbor was subjected to many factors which undoubtedly affected the rate of man-made litter ac-cumulation, especially, oil contamination (oil and old or new tar) which is a good es-timator of levels of oil contamination and an effective means of evaluating the poten-tial threat of oil on coastal resources. The results of the present study cleared out also that water temperature followed seasonal changes in air temperature. Aqaba Gulf water is characterized by its high salinity and the presence of well oxygenated waters. Minor changes in the distribution of pH, BOD, DOM and COD, revealed limited effects of human impacts. Low levels of Chl-a and TSM concentrations and high transparency revealed also negligible effects for human impacts. Significantly higher sea water temperatures, TSM, DO, BOD, DOM, COD, NH4, NO2, PO4 and TP were observed in summer season compared to their corresponding values in winter season. On contrast, higher values of chlorophyll-a, NO2, TN, and SiO4 were observed in winter compared to summer season. Based on the mean annual values, Aqaba Gulf coastal waters are classified as oligotrophic to mesotrophic state. The concentrations of dissolved inorganic nitrogen forms followed the following order: NH4= NO3＞NO2. In general, the majority of TN in winter was in the form of organic-N (96.3%) compared to 91.7% in summer season. On the meantime, TP exhibited more or less comparable organic-P percentage in summer (86.2%) and winter (81.2%) seasons. Moreover, the DIN/DIP ratio showed relatively higher values in winter season (14.2:1) compared to summer season (12.4:1). This indicates high nitrogen concentrations in comparison with that of phosphorous in winter; yet it reflects the generally lower nutrient levels present in the coastal waters of the Aqaba Gulf due to the lack of significant nutrient input or oceanic upwelling. Concentrations of heavy metals were relatively low signifying that Aqaba Gulf Coastal waters are not yet seriously threatened in spite of the rapid recreational and human developments that are considered a challenge to the safety and viability of Aqaba Gulf marine environment. Furthermore, concentrations of petroleum hydrocarbons were significantly higher in summer than in winter that are mainly attributed to the increase in the levels of oil pollution that most probably re-sulted from the increase in accidental, deliberate or operational discharges and spills of oil from ships. In general, the maximum concentration was much lower than the harmful concentrations reported for seawater. A stepwise multiple linear regression, analysis of variance (ANOVA), and principle component analysis (PCA) were applied.

Assessment of the Hydrochemical Characteristics of the Suez Gulf Coastal Waters during 2011-2013

The coastal region of the Suez Gulf, is one of the most densely industrialized zones in Egypt. In order to assess the quality of Suez Gulf coastal waters for the sustainable use and development, and consequently for the national income, 12 field campaigns were carried out in 3 years (2011-2013). The results of beach litter cleared out that the shoreline of the Suez Gulf, especially Ras Gharib area was subjected to many factors which undoubtedly affected the rate of man-made litter accumulation. The most abundant visible items were general litter as they represented 30.64% of the total items encountered. Oil, seaweeds as well as old and new tar constituted 15.41%, 14.85% and 11.84%, respectively. The increase on the levels of water temperature, Chl-a, TSM, DO, BOD, DOM, COD, ammonium ion, nitrite, nitrate, total phosphorus, silicate and total nitrogen developed a eutrophic region. In general, dissolved inorganic nitrogen (DIN) species were almost 9 times higher in the northern parts compared to the south-ern ones revealing that the northern parts of the Gulf were more affected by human impacts compared to the southern area. Moreover, concentrations of most studied metals such as Zn, Mn, Ni, Cu, Cr, Cd, Pb, and Hg were higher in Northern parts com-pared to southern parts of the Gulf. The concentrations of dissolved metals in the northern and southern Suez Gulf followed the following order: Fe ≥ Zn ≥ Cu > Pb > Mn > Ni > Cr > Cd ≥ Hg. Except for Hg, concentrations of metals were in the accepta-ble ranges recommended internationally. Hg concentrations especially in the northern Suez Gulf were slightly higher than the permissible levels. As the northern parts of the Gulf were more suffered from oil pollution, total petroleum hydrocarbons revealed higher concentrations in the northern parts compared to the southern parts of the Gulf. Seasonal variations, on the other hand, indicated higher levels of DO, BOD, DOM and COD, chloropyll-a, TSM, ammonia, nitrate, total nitrogen in winter compared to their corresponding values in summer. In general, the majority of TN and TP in winter were in the form of organic-N (87.2%) and organic P (94.1%). These percentages were 77.8% and 37.3%, respectively in summer. The surface coastal water of the Suez Gulf was principally, P-limited for phytoplankton growth in different investigated regions. Furthermore, the DIN/DIP ratio was higher (94.2:1) in winter season compared to summer season (6.3:1). Concentrations of Fe, Zn, Cd, and Hg in winter exhibited sig-nificantly higher values compared to their corresponding values in summer. On con-trast, Mn, Ni, Cu, Cr, and Pb exhibited significantly higher values in summer compared to their corresponding values in winter. Furthermore, concentrations of petroleum hy-drocarbons were significantly higher in winter than in summer which is mainly at-tributed to the increase in the rate of evaporation for petroleum hydrocarbons in summer due to the increase in water temperature and water salinity. In general, the maximum concentration was much lower than the harmful concentrations reported for seawater. A stepwise multiple linear regression, analysis of variance (ANOVA), and principle component analysis (PCA) were applied.

Arabian killifish (Aphanius dispar) embryos: A model organism for the risk assessment of the Arabian Gulf coastal waters.

Fish embryos are excellent models for studies aimed at understanding toxic mechanisms and indications of possible acute and chronic effects. For the past 3 yr, an Arabian killifish (Aphanius dispar) fish embryo test has been developed in the authors' laboratory as a routine ecotoxicological test that can be used to support risk assessment of potential contaminants in Arabian Gulf coastal waters. Tests were conducted with 3 reference toxicants (3,4-dichloroaniline [DCA], sodium dodecyl sulfate, and zinc sulfate [Zn]) and chlorine, a disinfectant used widely in industrial cooling systems around the Arabian Gulf region. The 50% effect concentration (EC50) for DCA was 0.47 mg/L and 1.89 mg/L for embryos exposed before 6 hpf and after 168 hpf, respectively. Sublethal effects were mainly observed at concentrations above 2.5 mg/L, the effects included severe pericardial edema and tail shortage. The sodium dodecyl sulfate ionic surfactant caused mortality at both early and late stages of embryo development; it caused coagulation, severe deformity, and hemolysis. Both the EC50 and the 50% lethal concentration (LC50) for sodium dodecyl sulfate were 9.37 mg/L. Salinity influenced the toxicity of Zn to killifish embryos: at 40 psu Zn was found not to be toxic, whereas at 20 psu toxicity had increased significantly (p < 0.05). Values of EC50 and LC50 were 2.5 mg/L and 4 mg/L, respectively. Concentrations above 15 mg/L in embryos were often accompanied by upper abdominal edema and inhibition of growth, especially evident in the tail. Chlorine caused mortality at a lower concentration; for example, at 0.05 mg/L 33% of embryos were found dead at the end of the experiment. The LC50 for chlorine was determined to be 0.08 mg/L. Examination of the existing literature showed similar results to the present study's findings. The results suggest a more comparable sensitivity of killifish embryos to that of other fish embryo test recommended species. The present study's findings support the ability of killifish to be an indicator organism for environmental risk assessments of Arabian Gulf waters. Benefits include sensitivity to a wide range of substances and conditions, animal alternative, ease of fish breeding, and clarity of the embryos.

Population structure, length-weight and length-length relationships of six populations of the Bartail Flathead Platycephalus indicus (Scorpaeniformes: Platycephalidae) along the Persian Gulf coastal waters

The size frequency distribution for age structure, length-weight (LWR) and length-length (LLR) relationships of six populations of Platycephalus indicus along the Persian Gulf coastal waters in Iran are reported. A total of 180 P. indicus specimens were studied from six localities including Charak, Bandar-Abbas, Shif, Motaf, Khur-Musa and Bahrekan. The maximum standard length recorded for the studied populations ranged between 300 to 510 mm and the b values of the length-weight relationships ranged between 3.0 and 3.2. The obtained results indicated isometric growth patterns for all these populations with the exception of the Bahrekan population which showed a positive allometric growth pattern. The LLR between the total and standard lengths in these populations were found to be highly significant. The results would be useful for further studies on population assessment and sustainable conservation of the fish along the Persian Gulf coastal waters.

Population structure, length-weight and length-length relationships of six populations of the Bartail Flathead Platycephalus indicus (Scorpaeniformes: Platycephalidae) along the Persian Gulf coastal waters

Population structure, length-weight and length-length relationships of six populations of the Bartail Flathead Platycephalus indicus (Scorpaeniformes: Platycephalidae) along the Persian Gulf coastal waters

Feeding habits of the king soldier bream, Argyrops spinifer (Forsskål, 1775) (Perciformes: Sparidae), in the northern Persian Gulf

Summary The feeding habits of the king soldier bream Argyrops spinifer (Forssk al, 1775), were examined in the northern part of the Persian Gulf (coastal waters of Bushehr, Iran). The stomach contents of 622 specimens, 13.5–64.2 cm in total length, collected by a trawl boat from June 2010 to May 2011, were analysed to assess the influence of size, season and sex on their diet composition. Amongst the examined stomachs, 57 were empty (vacuity index = 9.2%), which varied significantly (P < 0.05) throughout the year. According to the results, this species is a generalist carnivorous feeder with a diet consisting of 52 different prey items belonging to nine main categories. Bivalvia, Gastropoda, Malacostraca, Crustacea and Teleostei were the most important prey items. Fish size and gender showed no significant effects on diet composition. Moreover, in the main food catagories no seasonal variations were detected.

Natural and human influences on nutrient transport through a small subtropical Chinese estuary

Global understanding of land–ocean nutrient fluxes increasingly recognizes the disproportionate importance of small rivers. We studied nutrient fluxes from a small catchment in fast developing southern China to uncover effects of land-use. Water was sampled in the macro-tidal estuary of Nanliu River and adjacent Lianzhou Bay in spring and summer of investigate spatial and temporal variations of dissolved nutrients.High riverine concentrations of nitrate (NO3; up to 220μM) and phosphate (PO4; up to 3.7μM) mainly originated from agricultural fertilizer input. Riverine dissolved silica (Si; up to 47μM) increased in the oligosaline part of the estuary through human disturbance of bottom sediments. Dissolved organic nitrogen (DON; up to 194μM) and ammonium (NH4; up to 40μM) concentrations increased within the estuary due to inputs from livestock and mussel beds, respectively. Aquaculture ponds contained high concentrations of NH4 (up to 355μM) and DON (up to 151μM) but are not an important source to the estuary due to rare wastewater discharge and low absolute nutrient amounts relative to river export. Nutrient concentrations in Lianzhou Bay were low because tidal currents disperse land-derived nutrients offshore into the adjacent Beibu Gulf. A high proportion of regenerated nitrogen in the bay suggests that primary production is sustained by rapid in situ nutrient cycling between primary producers and benthic consumers. High nutrient export makes the Nanliu River an important nutrient source for the north-western South China Sea, despite its proportionately small size.Macro-tide induced short-term concentration changes exceed variability on seasonal and sub-seasonal scales. All nutrients vary inter-annually and between seasons, depending on precipitation-driven river runoff. Total nutrient export to Beibu Gulf coastal waters is stronger during the high discharge period in summer and autumn. In recent years changing nitrogen to phosphorus ratios have alleviated phosphorus limitation in Lianzhou Bay, permitting increased primary productivity.

The first report on some toxic effects of green scat, Scatophagus argus an Iranian Persian Gulf venomous fish

Green scat namely as Scatophagus argus is a venomous aquarium fish belonging to Scatophagidae family. It can induce painful wounds in injured hand with partial paralysis to whom that touch the spines. Dorsal and ventral rough spines contain cells that produce venom with toxic activities. According to unpublished data collected from local hospitals in southern coastal region of Iran, S. argus is reported as a venomous fish. Envenomation induces clinical symptoms such as local pain, partial paralysis, erythema and itching. In the present study green scat (spotted scat) was collected from Persian Gulf coastal waters. SDS-PAGE indicated 12 distinct bands in the venom ranged between 7 and 250 kDa. The crude venom had hemolytic activity on human erythrocytes (1%) with an LC100 (Lytic Concentration) of about 1.7 μg. The crude venom can release 813 μg protein from 0.5% casein. Phospholipase C activity was recorded at 3.125 μg of total venom. Our findings showed that the edematic activity remained over 24 h after injection. The results demonstrated that crude venom extracted from Iranian coastal border has different toxic and enzymatic activities. This study is pending to further investigation on animal model regarding protein purification and in vivo studies.

What are the potential drivers of blooms of the toxic dinoflagellate Karenia selliformis? A 10-year study in the Gulf of Gabes, Tunisia, southwestern Mediterranean Sea

We report on a 10-year study of the dynamics of toxic dinoflagellate Karenia selliformis at 20 stations along the nearshore of the Gulf of Gabes (Tunisia) in relation to environmental variables. Dinoflagellates and K. selliformis have dominated the phytoplankton community and K. selliformis blooms have taken place since 2001. Phytoplankton groups showed significant variability among sampling sites, but did not vary significantly from year to year. Redundancy analysis revealed that salinity, nitrate and phosphorus significantly explained the variability of the main phytoplanktonic groups namely, diatoms, dinoflagellates, cyanobacteria and euglenoids, while K. selliformis exhibited a specific pattern. Because of the wide variability in K. selliformis abundance, we tested the influence of environmental variables on its presence/absence using a generalized linear mixed-effect model (GLMM). K. selliformis occurrence had a positive relationship with nitrate and a negative one with total phosphorus. The different spatial gradients of these two chemical variables led to spatial differences in K. selliformis development prevalent near touristic areas. Temperature was also retained in the GLMM and since it rose over the 10 years of the study, it likely supports the expansion of K. selliformis. The discrepancies between model predictions and observed occurrences suggest that consideration of other sources of environmental forcing may improve our understanding of the determinism of K. selliformis dynamics. Our study may be useful in the management of this ecosystem so as to plan for the best disposal options in the treatment of urban and industrial wastes in the gulf's coastal waters.

Recruitment of marine biota onto hard and soft artificially created subtidal habitats in Sabah Al-Ahmad Sea City, Kuwait

Remediation of coastal habitats from impacts such as dredging and excavation in Gulf coastal waters is hampered by a lack of information on natural recolonisation rates and recruitment patterns of subtidal biota. For soft substrate habitats recovery information is only available for severely polluted sites where recovery takes many years (Jones et al., 2008).Construction of the Sabah Al-Ahmad Sea City provides a unique opportunity to follow benthic recruitment and community development on a range of artificially created benthic habitats over time. The three phases completed were each flooded by the sea separately and annual ecological surveys allow comparison of colonisation patterns and community development rates over time.Species diversity similar to that seen in comparable natural open sea habitats is reached within 2–5years for mixed sand/rock biota, but longer (2–6years) for sand biota. Biotic abundance exceeds open sea levels within 1–2years due to settlement of opportunistic species. Coral recruitment occurred within 3years. Present data provides a reference point for recovery rates into none polluted benthic habitats for the Gulf.