Guinea Pig Peritoneal Exudate Cells Research Articles

Contact hypersensitivity to simple chemicals. Incubation of sensitized guinea pig peritoneal exudate cells.

SummaryThe incubation of peritoneal exudate cells in Hanks balanced salt solution at 37 C, causes the release of a factor into the solution capable of specific transfer of contact sensitivity to DNFB. The release of this factor from 17-day cells appears to be complete since neither whole cells nor sonic extracts will transfer following incubation. After incubation, 13-day whole cells will transfer, yet sonic extracts of 13-day cells fail to transfer before or after incubation. The transfer factor is also released from 13-day cells in the presence of the specific DNP-protein conjugate, and in this case again, the release appears complete. DNP-protein conjugate does not cause the release of transfer factor from 17-day cells, nor does incubation in the presence of the conjugate interfere with transfer capability of the whole cell or, possibly, of the sonic extract as seen in one experiment. Transfer factor, when released, is specific and does not possess reactive capacities associated with classical antibody.

MEASUREMENT OF IMMUNE CYTOLYSIS.

IMMUNE lysis of tissue cells is more difficult to measure than immune haemolysis because the material lost by lysing cells is not coloured, as haemoglobin is. Greon, Fleischer, Barrow and Goldberg1 among others have shown that during immune lysis of ascites tumour cells, cytoplasmic nucleic acid is released from the cells, mainly as nucleoprotein. We have found it practicable to use this to measure cell lysis, and it is much easier than direct microscopic examination of the cells for measurement of damage2. Using this method it is possible to titrate both complement and cytolytic antibody against guinea pig peritoneal exudate cells (chiefly mononuclear cells) and Ehrlich ascites tumour cells with little more difficulty than comparable titrations using haemolysin and red cells involve.