Biocontrol agent, Nematodes, Pseudomonas sp. Biological control is considered as new efficient method that becomes widely used for controlling plant parasitic nematodes, as aim to decrease the extent of environment degradation and the effect of the excessive toxic nematicides. So, this study was done to investigate the role of some bacterial genera as biocontrol agent against Meloidogyne incognita. A number of bacterial species, isolated from root-knot nematodes conductive soil of different localities Guilan Province in Iran, were evaluated for suppression of Meloidogyne incognita. Nine isolates bacterial isolates significantly reduced nematode larvae population in soil belonging to Meloidogyne spp. Death percentage of nematode larvae ranged from 20.7% to 76.25%. Five potent bacterial isolates with higher nematocidal activity were selected and identified as Pseudomonas aeroginosae and Pseudomonas fluorescens using morphological and biochemical diagnosis tests The study indicated that Pseudomonas aeroginosae and Pseudomonas fluorescens were potent as bio-control agents for root-knot nematodes, the production of local Iran inoculums of both bacterial species as a safe bio-control agents for the root-knot disease