Guano Accumulation Research Articles

Time-transgressive microbial diversity in a tropical bat guano accumulation, Deer Cave, Mulu, Borneo

Bat guano accumulations in caves can constitute large deposits of organic material, particularly chitin and its degradation products, that host a diverse microbiome. Previous microbiological studies have not addressed the stratified nature of these deposits in the context of the timeframe of the decomposition process. We present such data (temperature, pH, DNA sampling of eight carbon-dated strata) for a 75 cm-deep tropical guano accumulation that undergoes complete decomposition over a timescale of ~120 years. The aerobic, surface layer of freshly-fallen guano has the greatest diversity of taxa, with Bacteroidota co-dominant with Pseudomonadota, and includes many taxa unique to this zone (including those from guanophagic invertebrates, bat guts, and insects). The ~20-40-year-old, more anaerobic, middle layer of increasing acidity and temperature has fewer taxa, with Pseudomonadota dominant and Bacteroidota almost disappearing, and many taxa tolerant of high temperatures and anaerobic conditions. The ~60-120-year-old, deepest, zone has the lowest diversity with a single genus of Actinomycetota (Mycobacterium) taking over as dominant. We also present data on the significantly different microbiomes of guano falling into aerobic versus water-logged, anaerobic conditions at the surface, the anaerobic having almost twice the diversity of the aerobic, including many taxa that are unique to this sample. Taxa of especial interest include methanogens, sulphur reducers and oxidizers, chitin decomposers, halophiles, acidophiles, and extremophiles.

Chemical and Physical Characterization of Swift Guano in Quartzitic Karst Landscape in Brazil

The Private Reserve of Natural Patrimony (RPPN) of the Santuário of Caraça is found in a mountain region within the Quadrilátero Ferrífero geological province in the State of Minas Gerais. In this region, intense metamorphic processes shaped their siliciclastic rocks, enabling the formation of fractures and diaclasis, the favourite routes of weathering during the development of karst landscapes. The natural cavities thus formed, mainly in the RPPN sector called “Pico do Inficionado”, provide shelter for countless swifts of the species Streptoprocne zonaris and S. biscutata, responsible for the accumulation of guano on the floor. However, guano is a substance rich in nutrients, mainly phosphates and nitrates, and this is the main nutrient supply in permanently dry caves. This work studied the physical (density) and chemical (organic carbon, nitrogen, potassium, phosphorus, sodium, calcium, magnesium, aluminium and pH) characteristics of 21 guanos layers, correlating their contents with the depth of seven deposits and separating the results into factors. Considering that, knowing that the guano deposits contain crucial elements such as Ca, Mg, and especially P and N, which act as an energy source for many chemotrophic organisms, the subdivision of the studied elements allowed us to defer three factors: the first one features the concentrations of Al, P, K, Na and CO, the second one was determined for N, pH and density. Finally, the third factor was based on Ca and Mg. The quartzitic material influences the chemical and physical composition of the deepest guano. As the rock changes, elements like Al and K are released and enrich the deeper layers, as well as increasing density due to the presence of sand in the material.

Soil-landscape interplays at Harmony Point, Nelson Island, Maritime Antarctica: Chemistry, mineralogy and classification

Soils and landforms of Nelson Island remain one of the least studied in the South Shetlands Archipelago, despite that it is one of the oldest ice-free areas and is strongly vegetated. In this paper, we examine the main processes and factors of soil formation at Harmony Point and the relation of soils to landforms, vegetation and lithology.To achieve the goals, 26 pedons were collected and studied from a 4 km2 ice-free area at Harmony Point (S62°18′; W059°10′) on the southern area of Nelson Island (Maritime Antarctica). The soils were sampled on all representative local landforms, including three levels of uplifted marine terraces up to cryoplanated plateau, waterlogged depressions, rock felsenmeer, debris slopes and patterned ground, and a paraglacial border of the ice cap (270 m a.s.l). Sampling along the cryoplanated plateau was carried out along a gradient extending inland from the margins of the Ice Cap; and sampling of the marine terraces was performed along a chronosequence under varying bird-nesting influence and age. The main pedogenetic processes observed in this area are marked phosphatization, melanization from the accumulation of organic matter, and cryoturbation. Soil development varies from weakly developed, shallow, stony and cryoturbated to well-developed and organic-rich, phosphate soils with colors ranging from grayish to brown. The mineralogical composition of the clay fraction contains secondary minerals, indicating the active role of chemical weathering. Ornithogenic soils have mature phosphate minerals such as vivianite and taranakite, as well as poorly crystalline leucophosphite. Intensively cryoturbated soils are underlain by permafrost and are classified as Typic Haploturbels; polygonal soils are widespread on the cryoplanated plateau. Areas without permafrost were classified as Typic Gelorthents. Phosphatization is a dominant soil-forming process in this area and is associated with past and present-day guano accumulation by bird nesting and has led to the the development of deeper Ornithogenic Haplorthels. The ornithogenic soils occur at different topographic levels on the cryplanated platform and marine terraces. High P concentrations can be used as a proxy of the past nesting birds' activities, with far-reaching implications, especially with regards to vegetation growth and microbial activity and diversity.

Microbial biomass has a key role in immobilization of organic nitrogen in Russão II cave (Goiás, Brazil).

Trophic gradient (dissolved organic carbon (DOC); total nitrogen (TN)) and carbon and nitrogen of microbial biomass (MBC and MBN) were analyzed in Russão II cave (Brazil - 14º05'05.3"S 46º23'07.1"W). Two areas inside of the cave (Twilght Zone – TZ; Dark Zone –DZ) and one in surface (Epigean – Epg) were sampling. Microbial assimilation was evaluated by microbial quotient (MBC/DOC = qMic) and C/N microbial biomass ratio. Student’s t test was applied to verify significant difference data. There was a significant difference in DOC, TN and MBC in the three areas. The DOC ranged from 74.25 to 165.27 g kg-1, while TN ranged from 0.10 to 0.15 g kg-1. The highest DOC values were observed in Epg followed by TZ and DZ areas, respectively, evidencing a trophic gradient. DZ area showed the highest TN values, probably due to guano accumulation inside the cave. The most abundant element in guano is nitrogen (about 12%). Higher MBC values in Epg (812.63 mg Kg-1) and MBN values in DZ (83.25 mg Kg-1) were observed. However, there was not a significant difference in relation to MBN at the studied areas. The qMic and C/N were lower in DZ area. Vegetation presence all year in Epg influences the yield and quality of litter, thereby contributing to higher MBC levels. Microbial biomass is an important source of organic nitrogen which has been demonstrated by small variation among MBN and TN. qMic reflects the relation between microbial and organic carbon, suggesting a lower immobilization of DOC in microbial biomass and a higher rate of carbon mineralization. A lower immobilization rate of MBC in relation to MBN is also evidenced by the lower C/N. Microbial biomass is an important pool of organic nitrogen considering this lower ratio between C / N biomass and the largest amount of MBN inside the cave.

Biogeochemistry of ornithogenic soils in Coastal Antarctica

Ornithogenic soils are usually considered to be formed as a result of breeding activities by sea birds. These soils are widespread in polar regions and in Coastal Antarctica in particular. It is believed that the most important impact of birds on soil formation in such environments is accumulation of guano – an important source of chemical elements and energy. In this paper we discuss an alternative point of view. We hypothesized that not only and not so much accumulation of guano, but also other bird-formed products significantly affect soil formation in Coastal Antarctica. An intensive biogenic flux of calcium from marine to terrestrial ecosystems in the food-chain: plankton + microbenthos → Nacella concinna → Larus dominicanus → guano + pellets (Nacella concinna shells) → soil strongly influences soil formation in Argentina islands by significant increase of soil pH values. The role of coral algae as an important source of calcium for terrestrial ecosystems of the Coastal Antarctic was shown. Further promising research priorities in the field of calcium biogeochemistry in polar environments were described.

Guano core evidence of palaeoenvironmental change and Woodland Indian inhabitance in Fern Cave, Alabama, USA, from the mid‐Holocene to present

Bat guano cores have been used as a source of palaeoenvironmental information to aid in the reconstruction of past climates and vegetation. We collected a 104‐cm‐long (43 cm compacted) guano core from Fern Cave, Alabama, USA, that provided a c. 6000‐year record of guano accumulation. Pollen, nutrients (C, N, P) and stable isotopes (δ13C, δ15N) were measured on the guano core with the objective of reconstructing the environmental history of the area from the mid‐Holocene to present. Our data indicate that bats have utilized Fern Cave for at least 6000 years and that Woodland Indians also utilized the cave for a short period. A 3‐cm charcoal layer was dated to 2720±30 cal. a BP and inferred to be Woodland Indian in origin from microscopic inspection and thickness. Pollen and geochemical data showed that bat diets changed in the late Holocene possibly linked to food supply and climate changes. These results demonstrate that guano cores are a useful tool of palaeoenvironmental reconstruction when other forms of palaeorecords do not exist and can add to local archaeological information.

Windows on Antarctic soil–landscape relationships: comparison across selected regions of Antarctica

Abstract This paper brings together topographic cross-section ‘windows’ from across Antarctica to illustrate soil–landscapes from the margins of the polar plateau in the Transantarctic Mountains and McMurdo Dry Valleys, through East Antarctic coastal areas, to the northern Antarctic Peninsula Region. Soils identified range from Gelisols in the Ross Sea Region, through Gelisols and Entisols in coastal East Antarctica, to a mixture of Gelisols, Entisols, Spodosols and Inceptisols in the northern Antarctic Peninsula Region where permafrost is not ubiquitous. The relative impacts of the soil-forming factors are considered. At a continental scale climate is the main driver of the differences observed between soils in different areas. At local scales strong soil–topographic relationships are observed. Organisms, time and parent material are dominant influences on soil properties only in relatively localized situations. Organisms dominate in areas of organic matter or guano accumulation and time is a dominant influence on exceptionally old upland surfaces in the McMurdo Dry Valleys. The US Department of Agriculture’s Soil Taxonomy gives a useful overall appraisal of Antarctic soils; however, for detailed work, there is a need to introduce some new categories at subgroup level to better capture the range of soils described.

Implicações geomorfológicas e paleogeográficas das crostas fosfáticas do Arquipélago de São Pedro e São Paulo, Atlântico Norte

No Arquipélago de São Pedro e São Paulo, a deposição de excrementos da avifauna foi responsável pela fosfatização de suas rochas. Como feição mais característica, formaram-se crostas fosfáticas. Com base em metodologias pertinentes ao trabalho de campo (observação, descrição e coleta) e de laboratório (análises geoquímicas, mineralógicas e microscópicas), esse trabalho buscou caracterizar tais crostas e compreender suas implicações geomorfológicas e paleogeográficas. As crostas foram definidas como feições de acumulação de excrementos, ricos em fósforo, mineralizados sobre rochas homogêneas (peridotitos milonitizados). Depois de formadas, o comportamento hidrofóbico de tais crostas protegeu as rochas contra o avanço acelerado do perfil de alteração e da atuação de processos erosivos, tornando as encostas em que são encontradas mais íngremes e convexizadas. Ao contrário, onde a formação da crosta não foi verificada, as encostas encontram-se concavizadas e a rocha mais profundamente alterada. A presença de tais crostas também indica a existência de ambientes áridos pretéritos, diferentes das condições climáticas atuais, onde foi possível a acumulação do guano sobre a superfície.

A radiocarbon dated bat guano deposit from N.W. Romania: Implications for the timing of the Little Ice Age and Medieval Climate Anomaly

There is considerable interest in the potential of bat guano as an alternative record of palaeoclimate in regions that are devoid of more commonly utilised archives. In this study, designed originally to evaluate the potential of cave hosted bat guano to preserve temporal variations in the flux of cosmogenic 36Cl, it was found that the guano depositional history is strongly linked to climatic conditions. Radiocarbon measurements on a 2.7 metre long core of bat guano from Măgurici Cave, N.W. Romania indicate a maximum depositional age of 1195 AD for the base of the core. Deposition of the lowermost portion of the accumulation occurred during the Medieval Climate Anomaly. The cave roost was subsequently devoid of bats during a regional cold phase linked to the Little Ice Age, with bats returning when local temperatures increased. The rate of guano accumulation then appears to increase in tandem with anthropogenic warming. This indicates that bat occupation at this roost site in Măgurici Cave is strongly linked to regional climate variability, with habitation during warm periods, possibly associated with the abundance of insects upon which the bats feed. Comparison of large peaks in anthropogenic 14C and 36Cl production associated with nuclear weapons testing indicates downward migration of 36Cl, probably reflecting post-depositional migration within the guano deposit. Elevated 36Cl/Cl at the top of the core in comparison with modern atmospheric values may indicate recycling of bomb 36Cl in vegetation. Therefore, we show that while bat guano contains abundant atmospherically-derived chloride it has severe limitations as a potential archive of atmospherically-derived 36Cl (a solar proxy), because of post-depositional mobility. However, separation of organically bound chloride, or the use of an alternative cosmogenic isotope 10Be, in bat guano, may offer an unexploited solar proxy that contains contemporaneous environmental signals, such as stable isotopes (e.g. δ 13C) and pollen, in association with radiocarbon dating.

10.1007/s11491-008-1011-9

A detailed investigation of microstructures of the phosphorites from Christmas Island under a scanning electron microscope coupled with an analysis of their chemical composition revealed that both their structure and composition are quite similar to those of the granular and microgranular phosphorites on present-day continents, as well as those of the phosphorites on some of the Pacific guyots. Their composition, together with their geological position and interrelationships with the surrounding rocks, proves that the ornitogenic hypothesis based on the presumed guano accumulation followed by its transformation into phosphatic rock is not compatible with the field observations. Meanwhile, the problem of the source of the Fe and Mn impregnation in the phosphorites remains unresolved and needs further investigations.

On the origin of phosphorites from Christmas Island in the Indian Ocean

A detailed investigation of microstructures of the phosphorites from Christmas Island under a scanning electron microscope coupled with an analysis of their chemical composition revealed that both their structure and composition are quite similar to those of the granular and microgranular phosphorites on present-day continents, as well as those of the phosphorites on some of the Pacific guyots. Their composition, together with their geological position and interrelationships with the surrounding rocks, proves that the ornitogenic hypothesis based on the presumed guano accumulation followed by its transformation into phosphatic rock is not compatible with the field observations. Meanwhile, the problem of the source of the Fe and Mn impregnation in the phosphorites remains unresolved and needs further investigations.

Phosphogenesis on selected low atolls in the near-equatorial part of the Western Indian Ocean

The compositions, structures, and microstructures of different types of phosphorites and poorly phosphatized rocks from low atolls in the near-equatorial part of the Western Indian Ocean are described. The rocks were examined under optical and scanning microscopes using microprobing techniques and etching of selected samples with weak solvents as well as with the help of chemical analyses. It is proved that phosphorites have been formed owing to the uneven phosphatization of the primary carbonate rocks; the degree of their phosphatization ranges from traces to 40% P2O5. In the phosphorites, numerous organic remains were encountered; they included fragments of plankton, debris of tortoise shells, and coccoidal and filamentous bacteria-like formations. It is suggested that the phosphorites were formed due to the high local biological productivity over the outer edges of coral reefs and are not related to guano accumulation or to endoupwelling.

Micromorphology and site formation at Die Kelders Cave I, South Africa

The deposits of Die Kelders I were previously described and studied by Tankard & Schweitzer (1974, 1976) from the standpoint of classical granulometric analysis of sand from a coastal cave in order to infer the geological history of the cave and its environs. This paper supplements these earlier works by taking a more holistic approach toward site formation processes by including investigation of the biogenic and anthropogenic influences on the cave deposits and history. The study employs the technique of soil micromorphology, which is the study of resin-impregnated, undisturbed blocks of sediment and petrographic thin sections, in which sediments from all areas of the cave were examined. The study showed that diagenesis of the deposits in the eastern areas of the excavation resulted in decalcification, which in turn brought about slumping and compaction. Equivalent stratigraphic layers exposed in the western and central areas were only mildly decalcified and consequently, these sediments contain limestone rock fall and relatively abundant marine and terrestrial mollusks, the latter not dissimilar to the Late Stone Age (LSA) midden which covers these deposits. Thus, in spite of lowered and more distant shorelines, marine resources were exploited during Middle Stone Age (MSA) times. Observations from these calcareous units also clearly demonstrates that previously recognized “occupational horizons” (e.g. Layers 6, 8 and 10) can be resolved micromorphologically into several ephemeral events, such as burning/fire, redistribution of ashes by wind and water, and non-deposition; the latter is shown by phosphatic alteration of sediments exposed on former surfaces and accumulation of guano, or the presence of millimeter-thick truncation surfaces below which aeolian dust infiltrated. Both field and microscopic observations illustrate that the deposits in caves are highly variable from wall to center, and that excavations should not be localized in just one microenvironment of the cave site.

Carbon and Nitrogen Isotopic Signatures of Bat Guanos as Record of Past Environments.

Carbon and nitrogen isotope ratios were measured for various ecogeochemical samples relevant to bat guano ecosystems. In particular, ca. 800-year-old subfossil guano from Jackson's Bay Cave Compex, Jamaica, yielded ratios similar to the modern guano from other Jamaican bat caves but quite different from modern guano of the same area. Diagenetic change and differences in bat food habits were unlikely explanations for the observation. Instead, insects that feed on C4 and CAM plants were the main prey for the bats in present Jackson's Bay area, while the ultimate source of organic matter for bats in other Jamaican caves and for the bats that deposited guano in Jackson's Bay Great Cave ca. 800 years ago were C3 photosynthesis. We suggest that the isotopic data indicate that the surrounding environment experienced a significant mesic episode in the recent past. This mesic climate would have supported a large population of bats, which in turn would have accumulated significant quantities of guano. The subsequent return to the more xeric conditions prevailing today would have caused a drastic reduction in bat population size and effectively ended significant guano accumulation. Fossil guano from Carlsbad Caverns, New Mexico, U.S.A., suggested that native C3 plants might have been more abundant in Wisconsinan than today. Isotope analyses of old guanos from Bat Cave in Grand Canyon National Park, Arizona, U. S. A., found a possible implication that C4 photosynthesis might have had contributed a little more to the bats' diet in the cave before the construction of Glen Canyon Dam.