Blood GSH-Px Activity Research Articles

Effect of selenium and vitamin E on oxidative stress in lambs experimentally infected with Haemonchus contortus

The objective of this study was to evaluate oxidative stress in lambs experimentally infected with Haemonchus contortus and supplemented with selenium and vitamin E. Twenty male Corriedale lambs were divided into four experimental groups with five animals each: G1 consisted of animals infected and supplemented with 0.2 mg/kg of live weight (LW) sodium selenite by intramuscular injection (IM); G2 consisted of animals infected with larvae and supplemented with 0.2 mg/kg LW sodium selenite IM and 2000 IU per animal of Vitamin E IM; G3 consisted of animals infected with larvae and supplemented with 2000 IU per animal of Vitamin E IM; and G4 consisted of animals infected with larvae. The animals were infected orally with 500 H. contortus larvae (L3) every 48 hours for 20 days. For biochemical analyses and eggs per grams of feces (EPG) evaluation, blood and feces were both collected at zero (T0), 20 (T1), 40 (T2) and 60 (T3) days. The weight of the animals was also measured at these times. Lower TBARS values were observed in the supplemented animals compared to the control group. The groups supplemented with Selenium exhibited blood GSH-Px activity higher than that of non-supplemented animals. Supplementation with selenium provided greater antioxidant protection against oxidative stress generated from experimental infection of lambs with H. contortus.

Study on Hepatotoxicity on rats caused by crude extracts of totalsaikosaponins `and correlation with oxidative damage mechanism

To observe the degree of hepatotoxicity damage to rats and the correlation with oxidative damage mechanism after taking a large dose of crude extracts of total saikosaponins for a long time. According to 45-day toxicity experimental methods, rats were successively administrated with crude extracts of total saikosaponins. High, middle and low dose were respectively 100, 50, 10 mg x kg(-1) calculated by total saikosaponins. The general conditions were observed and the related index of liver function, the metabolism condition of lipids, the glycometabolism, the level of TBIL were determined and the histopathologic examination of hepatic tissue were examined. The content of total-SH in serum, the level of MDA, the activity of SOD and the content and activity of GSH and GSH-Px in serum and liver tissue were detected. The crude extracts of total saikosaponins can increase the activity of ALT and AST in blood, increase liver weight and the ratio of liver to body, increase the content of TG in blood and liver. The effect on CHO in blood and Gn in liver is not obvious. Only high dose group's effect on TBIL can be observed. The hepatic cell damage is obvious in the histopathologic examination of hepatic tissue. The crude extracts of total saikosaponins can increase the content of total SH in blood, the content of MDA and GSH in blood and liver, while decrease the activity of SOD and GSH-Px in blood and liver. The above-mentioned changes gradually aggravates with dose increasing, and is obvious compared with distilled water control group. The crude extracts of total saikosaponinscan induce hepatotoxicity damage, and the mechanism of hepatic damage is related with oxidative damage mechanism.

Selenium and iodine status of sheep in the Markazi province, Iran

Summary The aim of this study was to provide preliminary quantitative information on selenium and iodine status of sheep in Markazi province, Iran. Selenium and iodine status of grazing sheep were measured for 57 different flocks in 14 regions over one year. The districts were selected to represent major sheep growing areas in the province. Blood samples were collected from 2 to 3-year-old ewes during summer, autumn and winter. Samples were analyzed for blood glutathione peroxidase (GSHpx) activity and plasma inorganic iodine. There were significant differences between regions and seasonal differences in terms of blood GSHpx activity and plasma inorganic iodine concentration (P<0.01). Low levels of GSHpx activity (<60 IU/gHb) and plasma inorganic iodine (<5 µg/dl) in some regions or some seasons indicated the need for dietary supplementation of these minerals.

Role of Selenium on Calcium Signaling and Oxidative Stress-induced Molecular Pathways in Epilepsy

Epilepsy is one of the oldest neurological conditions known to humankind. It is known that oxidative stress and generation of reactive oxygen species are a cause and consequence of epileptic seizures. Although recent years have seen tremendous progress in the molecular biology and metabolism of selenium, we still know little about the cell type-specific and temporal pattern of selenium and its derivatives in the brain of epileptic humans and experimental animals. It has been suggested that some antiepileptic drug therapies such as valproic acid, deplete the total body selenium level and selenium-dependent glutathione peroxidase (GSH-Px) activity although therapy with a new epileptic drug, topiramate, activated GSH-Px activity in epileptic animals and humans. An observation of lower blood or tissue selenium level and GSH-Px activity in epileptic patients and animals compared to controls in recent publications may support the proposed crucial role of selenium level and GSH-Px activity in the pathogenesis of epilepsy. Selenium is incorporated into an interesting class of molecules known as selenoproteins that contain the modified amino acid, selenocysteine. There are signs of selenium and selenoprotein deficiency in the pathogenesis of epilepsy. In conclusion, there is convincing evidence for the proposed crucial role of selenium and deficiency of GSH-Px enzyme activity in epilepsy pathogenesis. Blood GSH-Px activities could be a reliable indicator of selenium deficiency in patients with epilepsy.

Selenium metabolism in goats - maternal transfer of selenium to newborn kids

The aim of our study was to compare the concentration of selenium (Se) and the activity of glutathione peroxidase (GSH-Px) in the whole blood of goats and their newborn kids. The experiment involved 25 gravid, clinically healthy goats of the white shorthaired breed. On the day of delivery, we took whole blood from the mother and her newborn kid before the kid drank the first colostrum. In mothers, the measured average concentration of Se in whole blood was 149.60 ± 45.01 &amp;mu;g/l, the average concentration of Se in kids was 87.91 ± 29.66 &amp;mu;g/l. Average activity of GSH-Px in the blood of mothers was 938.46 ± 341.09 &amp;mu;kat/l, and in the blood of kids 658.20 ± 339.13 &amp;mu;kat/l. Regression and correlation analysis produced regression line formulas and correlation coefficients that revealed a close, statistically significant relation (&lt;I&gt;P&lt;/I&gt; &lt; 0.01) between the concentration of Se in the blood of mothers and their kids and the activity of GSH-Px in mothers and their kids. The relation between the concentration of Se in the blood (&amp;mu;g/l) of mothers and kids was &amp;gamma;= 0.484&lt;I&gt;x&lt;/I&gt; + 15.55; &lt;I&gt;r&lt;/I&gt; = 0.73, the relation between the activity of GSH-Px in blood (&amp;mu;kat/l) of mothers and their kids was &amp;gamma; = 0.809&lt;I&gt;x&lt;/I&gt; – 101.27; &lt;I&gt;r&lt;/I&gt; = 0.80. The concentration of Se and activity of GSH-Px was lower in newborn kids than in their mothers, reaching approximately 60–70% of the mother’s levels. The relation between the concentration of Se and activity of GSH-Px in the blood of goats was &amp;gamma; = 4.23&lt;I&gt;x&lt;/I&gt; + 276.31; &lt;I&gt;r&lt;/I&gt; = 0.64 (&lt;I&gt;P&lt;/I&gt; &lt; 0.01) and the relation between the concentration of Se and the activity of GSH-Px in the blood of kids was &amp;gamma; = 6.556&lt;I&gt;x&lt;/I&gt; + 64.70; &lt;I&gt;r&lt;/I&gt; = 0.83 (&lt;I&gt;P&lt;/I&gt; &lt; 0.01). It follows that a Se concentration of 100 &amp;mu;g/l corresponds to a GSH-Px activity of 699.51 &amp;mu;kat/l in the blood of mothers and 720.34 &amp;mu;kat/l in the blood of kids. The results show the need to provide for a sufficient Se saturation of goats with a view to preventing Se deficiency in kids and that the Se concentration in the blood of newborn kids is physiologically about 40% lower in comparison with the Se concentration in the blood of their mothers. This should be taken into account when interpreting the results and assessing the reference values of Se concentration in the blood of kids.

Evaluation of the influence of melatonin implants during the gestation period in sheep from a selenium-deficient region

To evaluate the possible effect of melatonin implants on blood glutathione peroxidase (GSHPx) activity and in the prevention of selenium (Se)-responsive disorders in sheep from an Se-deficient region. Randomized controlled clinical trial. 100 Merino ewes. Ewes of the same age, parity, body weight, body condition, and reproductive and health history were randomly allotted to 1 of 2 groups (control and implanted) of 50 sheep each. Treatment consisted of implants of melatonin (18 g) administered SC in the pinna of the right ear 6 weeks prior to introduction of rams. The control group did not receive implants. Hematologic and serum biochemical analyses were performed at various points before and after treatment, in addition to determinations of erythrocyte mean corpuscular fragility (MCF) and blood GSHPx activity. The incidence of Se-responsive disorders in lambs was recorded in both groups. Hematologic and serum biochemical analyses yielded values within respective reference ranges for both groups. Significant differences between groups were evident in MCF at early mating (lower in the implanted group vs the control group) and in blood GSHPx activity at early mating, gestation, and early lambing (higher in the implanted group vs the control group). There were significantly fewer lambs with nutritional myodystrophy in the implanted versus the control group. Use of melatonin implants in sheep may improve reproductive performance and yield an earlier start of breeding season. The stimulating effect of melatonin on GSHPx activity may protect against oxidative damage during the first stage of gestation.

Effect of dietary selenium and vitamin E supplementation on productive and reproductive performance in rams

The studies were carried out on 32 rams of the ossimi breed (270 days of age). Sixteen rams (group 1) received a ration containing 0.2 ppm. Se , and 30 mg vit.E, the other 16 rams (group 2) received 0.5 ppm. Se, and 50 mg vit.E/kg feed mixture .The feeding test was conducted from 270 days until 360 days of age. During the experiment the rams were subjected to live evaluations, i.e. testes circumference, libido level, semen characteristics, as well as selenium concentration and glutathione peroxidase (GSH-Px) activity in blood serum and seminal plasma. Routine macroscopic and microscopic analyses of semen quality were accompanied by measurement of Se content and GSH-Px activity in blood and semen. The Se concentration in blood plasma, seminal fluid and spermatozoa was measured by fluorometric method, while the GSH-Px activity by method based on NADPH - coupled reaction .Comparing the results in animals of both groups , it was clear that the all were in good health conditions as ascertained by clinical examination. No significant variation was detected in body weight of animals of both groups. Significant elevation (p<0.05) in the values of semen conc., semen conc./ ejac , semen motion , sperm motility / ejac.as well as total sperms count were observed in animals of group 2 as compared to those of group 1 .

Activity of glutathione peroxidase (GSH-Px) in the blood of ewes and their lambs receiving the selenium-enriched unicellular alga Chlorella

The effect of supplementation of selenium inorganic and organic forms on the activity of glutathione peroxidase (GSH-Px) was investigated for 7–8 months in three groups of ewes (by five animals each) and in born lambs. The basal feed ration contained 55 &amp;mu;g Se, the ewes of experimental group E1 received a supplement of 180 &amp;mu;g Se in sodium selenite, and the ewes of experimental group E2 were administered a supplement of 180 &amp;mu;g selenium bound in the biomass of the alga &lt;I&gt;Chlorella&lt;/I&gt;. Control group C was without selenium supplement. The ewes were in the stage of non-pregnancy, pregnancy and lactation during the experiment. The average number of lambs born per ewe was 1.0 in groups C and E1 and 1.8 in group E2. Both forms of selenium supplementation resulted in higher activity of GSH-Px in the whole blood, higher content of Se in the blood serum (&lt;I&gt;P&lt;/I&gt; &lt; 0.001) and milk of ewes (&lt;I&gt;P&lt;/I&gt; &lt; 0.001). Average activity of GSH-Px in the whole blood of ewes was as follows: C 697.9 ± 179.3; E1 1 147.4 ± 181.5; E2 1 056.1 ± 267.5 U/g Hb (&lt;I&gt;P&lt;/I&gt; &lt; 0.001). It reached the highest values in the 5&lt;sup&gt;th&lt;/sup&gt; to 6&lt;sup&gt;th&lt;/sup&gt; month of Se supplementation while the activity decreased after parturition in connection with lactation. Higher utilisation of Se from the organic form, compared to the inorganic form, was reflected in a higher content of Se in the milk of group E2 ewes (&lt;I&gt;P&lt;/I&gt; &lt; 0.05). The positive effect of selenium supplementation of ewes contributed to higher activity of GSH-Px in the whole blood of their lambs (&lt;I&gt;P&lt;/I&gt; &lt; 0.001) and higher Se concentration in the blood serum (&lt;I&gt;P&lt;/I&gt; &lt; 0.01). The organic form of Se (group E2) was more efficient in this case. Average activity of GSH-Px in the whole blood of lambs was as follows: C 434.1 ± 70.6; E1 1 031.6 ± 172.3 and E2 1 055.6 ± 235.1 U/g Hb.

Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on selenium tissue distribution and meat quality in beef cattle1

The objective was to determine the concentration of total Se and the proportion of total Se comprised as selenomethionine (SeMet) and selenocysteine (SeCys) in postmortem tissues of beef cattle offered diets containing graded additions of selenized enriched yeast (SY; Saccharomyces cerevisiae CNCM I-3060) or sodium selenite (SS). Oxidative stability and tissue glutathione peroxidase (GSH-Px) activity of edible muscle tissue were assessed 10 d postmortem. Thirty-two beef cattle were offered, for a period of 112 d, a total mixed ration that had been supplemented with SY (0, 0.15, or 0.35 mg of Se/kg of DM) or SS (0.15 mg of Se/kg of DM). At enrollment (0 d) and at 28, 56, 84, and 112 d following enrollment, blood samples were taken for Se and Se species determination, as well as whole blood GSH-Px activity. At the end of the study beef cattle were killed and samples of heart, liver, kidney, and skeletal muscle (LM and psoas major) were retained for Se and Se species determination. Tissue GSH-Px activity and thiobarbituric acid reactive substances were determined in skeletal muscle tissue (LM only). The incorporation into the diet of ascending concentrations of Se as SY increased whole blood total Se and the proportion of total Se comprised as SeMet, as well as GSH-Px activity. There was also a dose-dependent response to the graded addition of SY on total Se and proportion of total Se as SeMet in all tissues and GSH-Px activity in skeletal muscle tissue. Furthermore, total Se concentration of whole blood and tissues was greater in those animals offered SY when compared with those receiving a comparable dose of SS, indicating an improvement in Se availability and tissue Se retention. Likewise, GSH-Px activity in whole blood and LM was greater in those animals offered SY when compared with those receiving a comparable dose of SS. However, these increases in tissue total Se and GSH-Px activity appeared to have little or no effect in meat oxidative stability.

Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs

Thirty-two wether lambs of Tan sheep were randomly assigned into four dietary treatment groups (eight per group) for an 8-wk study and then fed a basal diet deficient in Se (0.06 mg/kg) or diets supplemented to provide 0.10 mg/kg Se from sodium selenite, selenized yeast, and seleniumenriched probiotics, respectively. Blood samples were collected at d 0, 28, and 56 of the experiment and tissue samples were collected at experiment termination. Tissue and blood Se concentrations, blood glutathione peroxidase (GSH-Px) activities, and plasma interleukin levels were analyzed. The results showed that the concentrations of Se in the kidney, liver, and muscle increased in all of the supplemented groups (p < 0.01) compared with the control group. However, the Se concentrations in the kidney, liver, and muscle in the groups supplemented with Se yeast and Se-enriched probiotics were higher than those in the group supplemented with sodium selenite (p < 0.01). The activities of GSH-Px and the concentrations of Se in blood also increased in all of the supplemented groups during the period of supplementation (p < 0.01) compared with the control group. The activities of GSH-Px and the concentrations of Se in the whole blood of the lambs fed with selenized yeast and Se-enriched probiotics were higher than those of lambs fed with sodium selenite (p<0.01 or p<0.05). The concentrations of interleukin-1 and interleukin-2 in plasma significantly increased in all of the supplemented groups during the entire period of experiment (p<0.01) compared with the control group, but had no significant differences among all of the supplemented groups. In conclusion, a diet supplemented with Se for finishing lambs was able to increase the concentrations of Se in tissue and blood, activities of GSH-Px in blood, and levels of interleukins in plasma. Organic Se sources (selenized yeast and Se-enriched probiotics) were more effective than the inorganic Se source (sodium selenite) in increasing tissue and blood Se concentrations and blood GSH-Px activities of lambs. However, there were no significant differences in plasma interleukin levels of lambs between organic and inorganic Se sources.

Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs

Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs

Effects of Se supplementation on growth rate and blood parameters in lambs

Forty-eight Appenninica lambs 30 d old received during a 63 d period the same diet (dry unifeed) supplied 4 different mineral premixes differing for their Se sources and levels to obtain the following 4 treatments (on an as fed basis): Control group (T1) - 0mg/kg Se; T2 – 0.30mg/kg Se as sodium selenite; T3 – 0.30mg/kg and T4 – 0.45mg/kg Se as Se yeast (Saccharomyces cerevisiae). Biweekly all the animals were weighed and feed consumption recorded. Moreover, at start (t0) and successively each two weeks (t14, t28, t42, t56), all the animals were blood sampled in order to evaluate plasma and whole blood Se concentration and blood glutathione peroxidase (GSH-Px) and creatine phosphokinase (CPK) activities. Daily weight gain (averaging 166±6g/d) never differed between treatments. Se supplementation increased Se concentration in blood in relation to dietary level (p<0.05) and, compared to selenite, Se yeast seemed more effective to obtain higher concentration in whole blood (P<0.05) but not in plasma. Blood GSH-Px activity was higher in lambs receiving Se supplementation but was not influenced by Se dietary level or source. No effects could be find on blood CPK activity.

Effects of Tongluo Recipe on experimental diabetic peripheral neuropathy in rats

To investigate the effects of Tongluo Recipe (TLR), a compound Chinese herbal medicine, on experimental diabetic peripheral neuropathy in rats. Diabetes was induced by streptozocin in rats. Eight weeks later, the diabetic rats were assigned to 3 groups according to different body weights: untreated group, low-dose TLR-treated group and high-dose TLR-treated group. Eight normal rats were assigned to normal control group. Systemic and sciatic nerve intrinsic antioxidant enzyme activities, lipid peroxide levels, hemorheology and sciatic nerve conduction velocity and ultrastructure changes were evaluated. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were lower, and lipid peroxide levels were higher in plasma and sciatic nerve of the untreated group than those in the normal control group (P<0.05). Blood glucose and viscosity in the untreated group were higher than those in the normal control group. Sciatic nerve showed demyelination changes and decrease of conduction velocity. Vasa nervorum expanded and erythrocyte deposited in the capillary in the untreated group. TLR obviously improved sciatic nerve conduction velocity and ameliorated morphology changes, decreased blood viscosity, improved SOD and GSH-Px activities. TLR can obviously improve sciatic nerve function and ameliorate morphology changes in rats with diabetic peripheral neuropathy, probably through depleting free radical production, improving endothelial cell function and ameliorating hemorheology changes.

Oxidant/Antioxidant Status of Placenta, Blood, and Cord Blood Samples From Pregnant Women Supplemented With Iron

In this study, it was aimed to investigate oxidant/antioxidant status in placenta and in blood and cord blood samples from pregnant women supplemented with iron during pregnancy. For this purpose, 27 pregnant women at admission for delivery participated in the study. Fifteen of them did not take iron tablets and the others took oral iron supplements during pregnancy. Following delivery, part of the placenta and blood and cord blood samples were taken from the mothers. In these samples, oxidant parameters (malondialdehyde [MDA] levels and xanthine oxidase [XO] activities) and antioxidant parameters (antioxidant potential [AOP] values, superoxide dismutase [SOD], catalase [CAT] and glutathione peroxidase [GSH-Px] activities) were studied. It was found that MDA level and SOD activities increased significantly in the placentas from the iron-supplemented group as compared with those from the control group. We also observed that activities of SOD and XO enzymes in maternal erythrocytes, XO in cord blood erythrocytes and GSH-Px activities in cord blood plasma decreased significantly. However, activities of CAT and GSH-Px enzymes in cord blood erythrocytes and MDA levels in maternal plasma increased in the iron-supplemented group as compared with those from the control group. Increased MDA levels in the maternal plasma and the placenta in the iron-supplemented group suggests that iron supplementation may contribute to increased oxidative stress in women taking iron supplements during pregnancy.

Actividad sanguínea de superóxido dismutasa y glutatión peroxidasa en novillas a pastoreo

El objetivo de este estudio fue determinar el balance metabólico nutricional de Cu, Zn, Fe, Mn y Se, mediante la actividad sanguínea de SOD y GSH-Px y establecer la relación entre la concentración de Cu, Fe, Mn y Zn en el forraje y la actividad de SOD. Se tomaron 10 mL de sangre a 105 novillas seleccionadas en 15 rebaños lecheros de Caldas, Colombia (5º4' N y 75º3' O) y se tomaron muestras de forrajes para analizar Cu, Fe, Mn y Zn. El promedio de la actividad de SOD fue 1.390±1.299 U g-1 Hb, y estaba correlacionada con Cu, Mn y Fe en el forraje. La actividad promedio de GSH-Px fue 389±184 U g-1 Hb y fue observada una mayor frecuencia de valores deficitarios y bajo/marginales de 9%, habiendo sido más afectados los animales de zonas altas (&gt;2.000 msnm). Bajo estas condiciones, estos resultados permiten señalar que la SOD es una enzima que puede emplearse como indicador del balance metabólico nutricional de Cu, Mn y Fe en bovinos a pastoreo, no está clara todavía su relación con Zn. La actividad de GSH-Px indica deficiencias en el balance metabólico nutricional de Se, en bovinos a pastoreo.

The relationship between whole blood selenium (Se) concentration and the activity of the seleno-enzyme, glutathione peroxidase (GSH-Px, E.C.I.11.1.9) in camel ( Camelus dromedarius)

The concentration of Se and the activity of the enzyme glutathione peroxidase (GSH-Px) were investigated in the whole blood of camels. Variable concentrations of whole blood Se (0.025–0.043 μg/ml) and GSH-Px activity (6.32–18.64 EU/ml) were demonstrated in both sexes, with females having significantly higher ( P < 0.001 ) blood Se concentration and GSH-Px activity than males. A highly significant correlation ( r = 0.88 , P < 0.001 ) has been demonstrated between whole blood Se concentration and the activity of the enzyme GSH-Px.

Selenium Status of Horses in the Czech Republic

home Acta Veterinaria Brno Journal of the University of Veterinary Sciences Brno, Czech Republic Search:

셀레늄급원으로 셀레늄강화버섯 폐배지의 급여가 거세한우의 도체특성, 혈중 GSH-Px활성 및 조직내 셀레늄축적에 미치는 영향

본 연구는 셀레늄급원으로 셀레늄강화버섯 폐배지(Se-SMC)를 거세한우에 급여하였을 때, 도체특성, 혈중 GSH-Px활성 및 조직내 셀레늄축적에 미치는 영향을 조사하기 위하여 실시하였다. 본 실험에 사용된 셀레늄급원은 유기셀레늄강화버섯을 생산한 후 폐기되는 버섯폐배지를 활용하였으며, 일반폐배지(SMC)를 조합하여 실험사료의 셀레늄 농도를 0.1, 0.3, 0.6, 0.9 ppm(건물기준)의 4처리구로 설정하였다. 실험동물은 비육후기 거세한우 20두(평균체중 613 kg, 20 <TEX>${\sim}$</TEX> 24개월령)를 공시하여, 처리구간 5두씩 배치하여 실험사료를 12주간 급여하였다. 실험기간 중 채혈하여 혈중 셀레늄농도와 혈장내 GSH-Px활성을 측정하였고, 근육(등심, 후지)과 간내 셀레늄함량과 도체특성을 측정하기위해 시험 후 도축하였다. Se-SMC의 보충으로 증가된 셀레늄수즌은 건물섭취량과 증체량에 영향을 미치지 않았고, 높은 농도의 처리군에서 중독증상은 발견되지 않았다. 도체특성 또한 처리구별 유의적인 차이를 나타내지 않았다. 혈중 셀레늄농도와 혈장내 GSH-Px활성은 Se-SMC 급여수준이 증가함에 따라 직선적으로 유의하게 증가하였다(P<0.01). 근육과 간내 셀레늄함량은 후지 및 간에서 사료중 셀레늄함량이 증가함에 따라 각각 건물 g당 0.27, 0.37, 0.40, 0.46<TEX>$\mu$</TEX>g 및 0.79, 1.40, 2.39, 3.10 <TEX>$\mu$</TEX>g를 나타내어 유의하게 증가하였다(P<0.05). 하지만, 등심내 셀레늄함량은 처리구간 유의적인 차이를 보이지 않았다. 이상과 같이 Se-SMC에 존재하는 셀레늄은 비육후기 거세한우에서 혈중 셀레늄농도와 GSH-Px활성을 유의하게 증가시킬 뿐만 아니라, 조직내 셀레늄함량을 증가시켜 저렴한 생산비로 셀레늄강화 쇠고기의 생산이 가능할 것으로 판단된다. This study was conducted to determine effects of spent composts of Se-enriched mushrooms (Se-SMC) as the dietary selenium source on carcass characteristics, plasma glutathione peroxidase(GSH-Px) activity and Se deposition in finishing Hanwoo steers. In combination with both Se-SMC and normal SMC, experimental treatment diets were formulated to contain 0.1, 0.3, 0.6 and 0.9 ppm of Se on a dry matter basis. A total of 20 finishing Hanwoo steers (average BW = 613 kg, average age = 20 to 24 mo) were allotted to treatments in four groups of five steers per pen for 12 wk preceding slaughter. While the experiment is employed, blood samples were taken to analyze Se concentration and GSH-Px activity, and muscle and liver samples were collected for analyses of Se contents in their tissues after slaughter. DMl and BW gain were not affected by dietary Se level and any toxic symptoms in treatments with a higher level of Se were not observed. No differences were noted for carcass characteristics. Se concentration in whole blood and plasma GSH-Px activity were linearly increased with the increasing level of dietary Se (P < 0.01). Se content in the hind leg for Se-SMC supplemented groups significantly increased (P < 0.05) upon dietary Se level, with 0.27, 0.37, 0.40 and 0.46 !1g1g dry, respectively. However, Se content in the loin was not affected by dietary Se levels. Se content in the liver was significantly increased(P < 0.05) as dietary Se increased, with 0.79, 1.40, 2.39 and 3.10 !1g1g dry, respectively. These results suggested that Se in the Se-SMC was highly bioavailable, and Se-SMC might be used not only as an inexpensive way of providing Se for ruminants but also as another way of producing Se-fortified beef.

Selenium status and GSH-Px activity in semen and blood of boars at different ages used for artificial insemination.

This study was performed to determine the relationship between selenium (Se) content and Se-dependent glutathione peroxidase (GSH-Px) activity in blood and semen, and semen quality of boars at different age used in an artificial insemination (AI) station. Routine macroscopic and microscopic analyses of semen quality were accompanied by measurement of Se content and GSH-Px activity in blood and semen. The Se concentration in blood plasma, seminal fluid and spermatozoa was measured by fluorometric method, the GSH-Px activity by a method based on NADPH-coupled reaction. A total of 155 ejaculates and 58 blood samples were investigated. The results of this study showed that there was no direct relationship between the Se content and GSH-Px activity in blood plasma and semen, and semen quality of sexual matured boars. The mechanisms controlling Se content and GSH-Px activity in blood and semen seem to be independent. The age of boars as a differentiating factor for Se content and GSH-Px activity in blood and semen is possible. It is concluded that a determination of Se status and/or GSH-Px activity in organism before Se supplementation is indicated.

Selenium status of sheep and their lambs in the Northern Serbian province of Vojvodina

The blood plasma concentrations of selenium and plasma and whole blood GSH-Px activities were determined in 30 sheep on three farms (A=Kać, B=Ečka and C=Čoka) in marginally deficient areas of Vojvodina. Selenium concentrations in feedstuffs from the territories surrounding the studied farms were lower than 0.10 ppm in more than 90% of the samples. Plasma selenium concentrations in sheep from farms A, B and C were 83.44 ± 19.09 μg/L; 183.90 ± 37.10 μg/L and 82.14±25.09 μg/L respectively. Plasma GSH-Px activity was the lowest on farm B (0.70 ± 0.37 μkat/L), nonsignificantly higher on farm A (0.81 ± 0.40 μkat/L) and highest on farm C (1.66 ± 0.88 μkat/L). Mean whole blood GSH-Px activities ranged between 194.38 μkat/L (farm C) and 236.51 μkat/L (farm B). On farm A the blood plasma concentrations of selenium and plasma and whole blood GSH-Px activities were determined also in suckling lambs in relation to their mothers. Plasma selenium content in lambs (50.58 ± 10.06 μg/L) was lower than in adult sheep at the same location. Both plasma (0.81 ± 0.40 μkat/L) and whole blood (424.21 ± 117.19 μkat/L) GSH-Px activities were significantly higher in lambs compared to their dams.