Trophic Factors Research Articles

Integrating ATAC-seq and RNA-seq to identify differentially expressed genes with chromatin-accessible changes during photosynthetic establishment in Populus leaves.

Leaves are the primary photosynthetic organs, providing essential substances for tree growth. It is important to obtain an anatomical understanding and regulatory network analysis of leaf development. Here, we studied leaf development in Populus Nanlin895 along a development gradient from the newly emerged leaf from the shoot apex to the sixth leaf (L1 to L6) using anatomical observations and RNA-seq analysis. It indicated that mesophyll cells possess obvious vascular, palisade, and spongy tissue with distinct intercellular spaces after L3. Additionally, vacuoles fuse while epidermal cells expand to form pavement cells. RNA-seq analysis indicated that genes highly expressed in L1 and L2 were related to cell division and differentiation, while those highly expressed in L3 were enriched in photosynthesis. Therefore, we selected L1 and L3 to integrate ATAC-seq and RNA-seq and identified 735 differentially expressed genes (DEGs) with changes in chromatin accessibility regions within their promoters, of which 87 were transcription factors (TFs), such as ABI3VP1, AP-EREBP, MYB, NAC, and GRF. Motif enrichment analysis revealed potential regulatory functions for the DEGs through upstream TFs including TCP, bZIP, HD-ZIP, Dof, BBR-BPC, and MYB. Overall, our research provides a potential molecular foundation for regulatory network exploration in leaf development during photosynthesis establishment.

Antioxidant, Pro-Survival and Pro-Regenerative Effects of Conditioned Medium from Wharton's Jelly Mesenchymal Stem Cells on Developing Zebrafish Embryos.

Conditioned media harvested from stem cell culturing have the potential to be innovative therapeutic tools against various diseases, due to their high content of growth, trophic and protective factors. The evaluation in vivo of the effects and biosafety of these products is essential, and zebrafish provides an ideal platform for high-throughput toxicological analysis, concurrently allowing the minimization of the use of mammalian models without losing reliability. In this study, we assessed the biological effects elicited by the exposure of zebrafish embryos to a conditioned medium derived from Wharton's jelly mesenchymal stem cells. By a multiparametric investigation combining molecular, embryological, behavioural and in vivo imaging techniques, we found that exposure to a conditioned medium at a non-toxic/non-lethal dosage triggers antioxidant, anti-apoptotic and pro-regenerative effects, by upregulation of a set of genes involved in antioxidant defence (nrf2, brg1, sirt1, sirt6, foxO3a, sod2 and cat), glycolysis (ldha) and cell survival (bcl2l1, mcl1a and bim), coupled to downregulation of pro-apoptotic markers (baxa, caspase-3a and caspase-8). To our knowledge, this is the first study comprehensively addressing the effects of a conditioned medium on a whole organism from a developmental, molecular and behavioural perspective, and we are fairly confident that it will pave the way for future therapeutic application.

Amniotic Membrane and Its Derivatives: Novel Therapeutic Modalities in Liver Disorders.

The liver is a vital organ responsible for metabolic and digestive functions, protein synthesis, detoxification, and numerous other necessary functions. Various acute, chronic, and neoplastic disorders affect the liver and hamper its biological functions. Most of the untreated liver diseases lead to inflammation and fibrosis which develop into cirrhosis. The human amniotic membrane (hAM), the innermost layer of the fetal placenta, is composed of multiple layers that include growth-factor rich basement membrane, epithelial and mesenchymal stromal cell layers. hAM possesses distinct beneficial anti-fibrotic, anti-inflammatory and pro-regenerative properties via the secretion of multiple potent trophic factors and/or direct differentiation into hepatic cells which place hAM-based therapies as potential therapeutic strategies for the treatment of chronic liver diseases. Decellularized hAM is also an ideal scaffold for liver tissue engineering as this biocompatible niche provides an excellent milieu for cell proliferation and hepatocytic differentiation. Therefore, the current review discusses the therapeutic potential of hAM and its derivatives in providing therapeutic solutions for liver pathologies including acute liver failure, metabolic disorders, liver fibrosis as well as its application in liver tissue engineering.

Efferocytosis of viable versus heat-inactivated MSC induces human monocytes to distinct immunosuppressive phenotypes

BackgroundImmunomodulation by mesenchymal stromal cells (MSCs) can occur through trophic factor mechanisms, however, intravenously infused MSCs are rapidly cleared from the body yet a potent immunotherapeutic response is still observed. Recent work suggests that monocytes contribute to the clearance of MSCs via efferocytosis, the body’s natural mechanism for clearing dead and dying cells in a non-inflammatory manner. This begs the questions of how variations in MSC quality affect monocyte phenotype and if viable MSCs are even needed to elicit an immunosuppressive response.MethodsHerein, we sought to dissect MSC’s trophic mechanism from their efferocytic mechanisms and determine if the viability of MSCs prior to efferocytosis influences the resultant phenotype of monocytes. We cultured viable or heat-inactivated human umbilical cord MSCs with human peripheral blood mononuclear cells for 24 h and observed changes in monocyte surface marker expression and secretion profile. To isolate the effect of efferocytosis from MSC trophic factors, we used cell separation techniques to remove non-efferocytosed MSCs before challenging monocytes to suppress T-cells or respond to inflammatory stimuli. For all experiments, viable and heat-inactivated efferocytic-licensing of monocytes were compared to non-efferocytic-licensing control.ResultsWe found that monocytes efferocytose viable and heat-inactivated MSCs equally, but only viable MSC-licensed monocytes suppress activated T-cells and suppression occurred even after depletion of residual MSCs. This provides direct evidence that monocytes that efferocytose viable MSCs are immunosuppressive. Further characterization of monocytes after efferocytosis showed that uptake of viable-but not heat inactivated-MSC resulted in monocytes secreting IL-10 and producing kynurenine. When monocytes were challenged with LPS, IL-2, and IFN-γ to simulate sepsis, monocytes that had efferocytosed viable MSC had higher levels of IDO while monocytes that efferocytosed heat inactivated-MSCs produced the lowest levels of TNF-α.ConclusionCollectively, these studies show that the quality of MSCs efferocytosed by monocytes polarize monocytes toward distinctive immunosuppressive phenotypes and highlights the need to tailor MSC therapies for specific indications.

Therapeutic effects of different intervention forms of human umbilical cord mesenchymal stem cells in the treatment of osteoarthritis.

Osteoarthritis (OA) is a common and disabling disease. For advanced OA, surgical treatment is still the main treatment. Human umbilical cord mesenchymal stem cells (hUC-MSCs) are self-regenerative pluripotent cells, that coordinate cartilage regeneration by secreting various trophic factors, which adjust the injured tissue environment. hUC-MSCs secret extracellular vesicles and participates in OA treatment by transmitting bioactive molecules related to migration, proliferation, apoptosis, inflammatory reaction, extracellular matrix synthesis and cartilage repair. In addition, the combination of multiple substances represented by cartilage matrix and hUC-MSCs also have a significant synergistic effect on OA treatment. Because hUC-MSCs have shown considerable promise in cartilage repair, some scholars have proposed transplanting mesenchymal stem cells into damaged cartilage to delay OA progression. This article reviews the application of hUC-MSCs as a treatment for OA. With the continuous development of routine clinical applications, more reliable intervention modalities for hUC-MSCs in OA treatment will be discovered for the time to come.

Pre sowing dressing with plant growth substances for management of bakanae in aromatic rice (Oryza sativa L.) varieties

In this study, we evaluated the potential of seed priming with plant growth substances on morpho-physiological and yield attributes of bakanae infected seedlings of aromatic rice varieties Pusa Basmati 1121 and Pusa Basmati 1509. The study involved laboratory and field examination of seed priming for 6 h in SA at 5 mg L−1 &10 mg L−1 concentration, MeSA at 50 mg L−1 and 100 mg L−1 concentration, Prohexadione-Ca at 100 mg L−1 and 200 mg L−1 concentration, BABA at 5 mg L−1 and 10 mg L−1 concentration, followed by inoculation with the fourteen-day-old pure culture of Fusarium fujikuroi. The positive (inoculated hydroprimed seeds) and negative controls (un-inoculated untreated seeds) were maintained for both varieties simultaneously and the growth and physiological observations were made at 7, 14 and 21 DAG. The seed priming treatments recorded enhancement in germination, fresh and dry weight, vigour index, total chlorophyll and carotenoid content over the positive control. The optimum dose of each plant growth substance from laboratory evaluation was selected for the field studies. The procedure for priming and inoculation was same for field trial and observations were recorded at tillering, booting and anthesis stage. The seed priming treatments resulted in reduction in percent disease incidence and visible symptomology of bakanae concomitant with an enhancement in field emergence, biochemical attributes under field conditions. The study can be further used to decipher the biochemical and molecular mechanism underlying cross talk of plant growth substances during bakanae infestation in rice in order to design a disease management strategy.

Post-Mortem Studies of Neurturin Gene Therapy for Parkinson's Disease: Two Subjects with 10 Years CERE120 Delivery.

Neurturin is a member of the glial cell line-derived neurotrophic factor family of neurotrophic factors and has the potential to protectdegeneratingdopaminergic neurons. Here, we performed post-mortem studies on two patients with advanced Parkinson's disease that survived 10 years following AAV-neurturin gene (Cere120) delivery to verify long-term effects of trophic factor neurturin. Cere120 was delivered to the putamen bilaterally in one case and to the putamen plus substantia nigra bilaterally in the second. Immunohistochemistry was used to examine neurturin, Rearranged during transfection(RET), phosphor-S6, and tyrosine hydroxylase expressions, inflammatory reactions, and α-synuclein accumulation. In both patients there was persistent, albeit limited, neurturin expression in the putamen covering 1.31% to 5.92% of the putamen. Dense staining of tyrosine hydroxylase-positive fibers was observed in areas that contained detectable neurturin expression. In substantia nigra, neurturin expression was detected in 11% of remaining melanin-containing neurons in the patient with combined putamenal and nigral gene delivery, but not in the patient with putamenal gene delivery alone. Tyrosine hydroxylase positive neurons were 66% to 84% of remaining neuromelanin neurons in substantia nigra with Cere120 delivery and 23% to 24% in substantia nigra without gene delivery. More RET and phosphor-S6 positive neurons were observed in substantia nigra following nigral Cere120. Inflammatory and Lewy pathologies were similar in substantia nigra with or without Cere120 delivery. This study provides evidence of long-term persistent transgene expression and bioactivity following gene delivery to the nigrostriatal system. Therefore, future efforts using gene therapy for neurodegenerative diseases should consider means to enhance remaining dopamine neuron function and stop pathological propagation. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.

The biological invasion of an apex predator (Silurus glanis) amplifies PCB transfer in a large lake food web

Invasive species can affect food web structure possibly modifying the transfer of pollutants in ecosystems but this facet of biological invasion remains largely unexplored. We examined how trophic and ontogenetic characteristics of the invasive European catfish could differ from its native counterpart, the Northern pike, possibly resulting in the amplification of PCB transfer to the higher trophic levels in a large lake food web. The PCB contents of catfish and pike were on average low (Ʃ7 PCBi 42.4 ± 38.6 ng g−1 ww and 37.9 ± 49.4 ng g−1 ww respectively) and dominated by PCB153 (~35 % of the PCB contamination). Only the largest pike (126 cm) slightly exceeded the European sanitary threshold of 125 ng g−1 ww Ʃ6 PCBi-NDL. Both species increased in trophic position with body size while catfish had clearly higher littoral reliance than pike indicating they exploited complementary trophic niches. PCB biomagnification was identified only for catfish (PCB153, Ʃ7 PCBi) leading to trophic magnification factor of ~5. PCB ontogenetic bioaccumulation was pervasive for catfish (PCB101, PCB118, PCB153, PCB138 and Ʃ7 PCBi) and identified for pike only regarding PCB101. The derived size accumulation factors (~1.02) indicated a size-doubling PCB contamination of ~40 cm for catfish. This finding suggested that catfish would exceed the European sanitary threshold at body size larger than 168 cm possibly constraining their commercial exploitation. Our results highlighted that the invasive catfish was a littoral-oriented apex predator occupying an alternative trophic niche as compared to pike thereby modifying the lake food web structure that resulted in an enhancement of PCB transfer to higher trophic levels. The biomagnification and ontogenetic bioaccumulation of catfish underlined the impact of this biological invasion on the fate of PCB in the ecosystem. Finally, the remarkable inter-individual PCB contamination suggested variable inter-individual PCB exposure likely associated to localized hotspots of PCB contamination in the lake.

Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures.

Mesenchymal stromal cell (MSC)-derived products, such as trophic factors (MTFs), have anti-inflammatory properties that make them attractive for cell-free treatment. Three-dimensional (3D) culture can enhance these properties, and large-scale expansion using a bioreactor can reduce manufacturing costs. Three lots of MTFs were obtained from umbilical cord MSCs produced by either monolayer culture (Monol MTF) or using a 3D microcarrier in a spinner flask dynamic system (Bioreactor MTF). The resulting MTFs were tested and compared using anti-inflammatory potency assays in two different systems: (1) a phytohemagglutinin-activated peripheral blood mononuclear cell (PBMNC) system and (2) a lipopolysaccharide (LPS)-activated macrophage system. Cytokine expression by macrophages was measured via RT-PCR. The production costs of hypothetical units of anti-inflammatory effects were calculated using the percentage of TNF-α inhibition by MTF exposure. Bioreactor MTFs had a higher inhibitory effect on TNF (p < 0.01) than monolayer MTFs (p < 0.05). The anti-inflammatory effect of Bioreactor MTFs on IL-1β, TNF-α, IL-8, IL-6, and MIP-1 was significantly higher than that of monolayer MTFs. The production cost of 1% inhibition of TNF-α was 11-40% higher using monolayer culture compared to bioreactor-derived MTFs. A 3D dynamic culture was, therefore, able to produce high-quality MTFs, with robust anti-inflammatory properties, more efficiently than monolayer static systems.

Occurrence and accumulation of microplastics in commercial fish in the coastal waters of the Lvsi fishing ground in China

In recent years, there has been an exponential increase in the research popularity of microplastics (MPs) in offshore marine environments. However, there is still a gap in the research on the accumulation of MPs in different tissues of aquatic organisms and the trophic transfer of MPs between aquatic organisms. The common occurrence of MPs in the gills and guts of 11 species of commercial fishes was examined in the coastal waters of the Lvsi fishing ground (LSFG). The obtained results showed that >85 % of MPs existed in the gills and guts of these fish, and the abundance was 2.39 ± 1.38 pieces/fish and 2.56 ± 1.42 pieces/fish, respectively. Fibrous and blue are the most common colors and shapes of MPs, and PET is the main polymer type. At the species level, the abundance of MPs in the gills and guts of a few fishes (e.g., Larimichthys polyactis, Setipinna tenuifilis, Collichthys lucidus) decreased with increasing body length and body weight (P < 0.05). At the community level, this situation was not significant (P > 0.05). With increasing trophic level (TL), MPs tended to decrease in the gills (trophic magnification factor, TMF = 0.86) but did not significantly vary in the gut. We believe that MPs are multidimensional pollutants, and their accumulation in tissues/organs of organisms has not been accurately and qualitatively determined. To establish the relationship of MP transport and trophic transfer among water, sediments and organisms, we suggest that more efforts should be made to investigate MPs in aquatic organisms in the coastal waters of LSFG and to increase the examination of MPs in the water column and sediments. This study will help us improve our understanding of MPs pollution, and provide a good reference and basis for the management, monitoring and implementation of pollutants in marine organism of coastal water.

Болотные ресурсы Горного Алтая и подходы к их рациональному использованию

The article presents the results of a survey of the territory of the Altai Republic, in order to identify peat swamps and paludified soils. The general technical, agrochemical and chemical properties of peat were determined and radiocarbon dating of peat deposits The properties of peat suggest their application in agriculture for the production of organic fertilizers, biostimulants and growth substances, and can also be used in balneology and medicine. It was proposed to distribute the peat fund of the Altai Republic among trust funds with the allocation of a protected fund as a priority.

The Secretome of Human Dental Pulp Stem Cells and Its Components GDF15 and HB-EGF Protect Amyotrophic Lateral Sclerosis Motoneurons against Death.

Amyotrophic lateral sclerosis (ALS) is a fatal and incurable paralytic disorder caused by the progressive death of upper and lower motoneurons. Although numerous strategies have been developed to slow disease progression and improve life quality, to date only a few therapeutic treatments are available with still unsatisfactory therapeutic benefits. The secretome of dental pulp stem cells (DPSCs) contains numerous neurotrophic factors that could promote motoneuron survival. Accordingly, DPSCs confer neuroprotective benefits to the SOD1G93A mouse model of ALS. However, the mode of action of DPSC secretome on motoneurons remains largely unknown. Here, we used conditioned medium of human DPSCs (DPSCs-CM) and assessed its effect on survival, axonal length, and electrical activity of cultured wildtype and SOD1G93A motoneurons. To further understand the role of individual factors secreted by DPSCs and to circumvent the secretome variability bias, we focused on GDF15 and HB-EGF whose neuroprotective properties remain elusive in the ALS pathogenic context. DPSCs-CM rescues motoneurons from trophic factor deprivation-induced death, promotes axon outgrowth of wildtype but not SOD1G93A mutant motoneurons, and has no impact on the spontaneous electrical activity of wildtype or mutant motoneurons. Both GDF15 and HB-EGF protect SOD1G93A motoneurons against nitric oxide-induced death, but not against death induced by trophic factor deprivation. GDF15 and HB-EGF receptors were found to be expressed in the spinal cord, with a two-fold increase in expression for the GDF15 low-affinity receptor in SOD1G93A mice. Therefore, the secretome of DPSCs appears as a new potential therapeutic candidate for ALS.

Metal-specific biomagnification and trophic dilution in the coastal foodweb of the California sea lion (Zalophus californianus) off Bahía Magdalena, Mexico: The role of the benthic-pelagic foodweb in the trophic transfer of trace and toxic metals

Trace metals concentrations along with stable isotopes ratios were measured in marine algae, sea grass, sponges, echinoderms, mollusks, crustaceans, fishes, and the California sea lion, to assess the bioaccumulation potential and detect potential risks for top predators off Bahia Magdalena, Mexico. We assessed the trophic magnification factor (TMF) to determine the potential for biomagnification of 11 trace metals. The concentrations of Fe and Zn were one order of magnitude higher than all other metals. Concentrations of As, Cu, Cd, Co, Cr, Fe, Mn and Ni correlated negatively with trophic level, supporting trophic dilution (TMF < 1, p > 0.05), while Zn and Hg had significant trophic magnification (TMF > 1, p < 0.05) when assessing only the benthic-pelagic foodweb. This research provides a baseline concentration of metals in multiple species, metal-specific foodweb bioaccumulation and biomagnification of mercury, underscoring the key role of the macrobenthic community as biovectors for trophic transfer of Hg through the foodweb to the California sea lion.

AAV5-mediated manipulation of insulin expression in choroid plexus has long-term metabolic and behavioral consequences

The choroid plexus (CP) is a source of trophic factors for the developing and mature brain. Insulin is produced in epithelial cells of the CP (EChPs), and its secretion is stimulated by Htr2c-mediated signaling. We modulated insulin expression in EChPs with intracerebroventricular injections of AAV5. Insulin overexpression in CP decelerates food intake, whereas its knockdown has the opposite effect. Insulin overexpression also results in reduced anxious behavior. Transcriptomic changes in the hypothalamus, especially in synapse-related processes, are also seen in mice overexpressing insulin in CP. Last, activation of Gq signaling in CP leads to acute Akt phosphorylation in neurons of the arcuate nucleus, indicating a direct action of CP-derived insulin on the hypothalamus. Taken together, our findings signify that CP is a relevant source of insulin in the central nervous system and that CP-derived insulin should be taken into consideration in future work pertaining to insulin actions in the brain.

Human retinal secretome: A cross-link between mesenchymal and retinal cells.

In recent years, mesenchymal stem cells (MSC) have been considered the most effective source for regenerative medicine, especially due to released soluble paracrine bioactive components and extracellular vesicles. These factors, collectively called the secretome, play crucial roles in immunomodulation and in improving survival and regeneration capabilities of injured tissue. Recently, there has been a growing interest in the secretome released by retinal cytotypes, especially retinal pigment epithelium and Müller glia cells. The latter trophic factors represent the key to preserving morphofunctional integrity of the retina, regulating biological pathways involved in survival, function and responding to injury. Furthermore, these factors can play a pivotal role in onset and progression of retinal diseases after damage of cell secretory function. In this review, we delineated the importance of cross-talk between MSCs and retinal cells, focusing on common/induced secreted factors, during experimental therapy for retinal diseases. The cross-link between the MSC and retinal cell secretomes suggests that the MSC secretome can modulate the retinal cell secretome and vice versa. For example, the MSC secretome can protect retinal cells from degeneration by reducing oxidative stress, autophagy and programmed cell death. Conversely, the retinal cell secretome can influence the MSC secretome by inducing changes in MSC gene expression and phenotype.

Organophosphate diesters (DAPs) and hydroxylated organophosphate flame retardants (HO-OPFRs) as biomarkers of OPFR contamination in a typical freshwater food chain

Organophosphate flame retardants (OPFRs) can rapidly biotransform into two types of metabolites in biota: (1) organophosphate diesters (DAPs) and (2) hydroxylated OPFRs (HO-OPFRs). Therefore, the levels of parent OPFRs alone are not sufficient to indicate OPFR pollution in biological organisms. This study analyzed 12 OPFR metabolites, including 6 DAPs and 6 HO-OPFRs, in a typical freshwater food chain consisted of crucian carp, catfish, mud carp, snakehead, and oriental river prawn. The total concentrations of OPFR metabolites were comparable to those of parent OPFRs, and ranged from 0.65 to 17 ng/g ww. Bis(2-butoxyethyl) 3′-hydroxy-2-butoxyethyl phosphate (14%–77%), di-n-butyl phosphate (DNBP) (6.7%–24%), bis(1-chloro-2-propyl) phosphate (BCIPP) (0.7%–35%), and 1-hydroxy-2-propyl bis(1-chloro-2-propyl) phosphate (BCIPHIPP) (6.0%–24%) were the major OPFR metabolites. Various aquatic species exhibited significant differences in their OPFR metabolite/parent ratios (MPR) (p < 0.05), indicating varying biotransformation potentials of different organisms for various OPFRs. The growth-independent accumulation of tri-n-butyl phosphate (TNBP), tris(chloro-2-propyl) phosphate (TCIPP), triphenyl phosphate, and 2-ethylhexyl diphenyl phosphate in mud carps could be explained by their biotransformation potential. A significant negative correlation was found between the concentration of bis(2-butoxyethyl) phosphate and δ15N values (p < 0.05), with a calculated trophic magnification factor (TMF) of 0.66. Significant positive correlations were observed between BCIPP and TCIPP (R2 = 0.25, p < 0.05), as well as between DNBP and TNBP (R2 = 0.30, p < 0.01), implying that these two DAPs could be used as biomarkers to quantitatively assess TCIPP and TNBP contamination in wild aquatic organisms.

From Teeth to Therapy: A Review of Therapeutic Potential within the Secretome of Stem Cells from Human Exfoliated Deciduous Teeth.

Stem cells derived from human exfoliated deciduous teeth (SHED) have emerged as an alternative stem cell source for cell therapy and regenerative medicine because they are readily available, pose fewer ethical concerns, and have low immunogenicity and tumourigenicity. SHED offer a number of advantages over other dental stem cells, including a high proliferation rate with the potential to differentiate into multiple developmental lineages. The therapeutic effects of SHED are mediated by multiple mechanisms, including immunomodulation, angiogenesis, neurogenesis, osteogenesis, and adipogenesis. In recent years, there is ample evidence that the mechanism of action of SHED is mainly due to its paracrine action, releasing a wide range of soluble factors such as cytokines, chemokines, and trophic factors (also known as 'secretome') into the local tissue microenvironment to promote tissue survival and recovery. This review provides an overview of the secretome derived from SHED and highlights the bioactive molecules involved in tissue regeneration and their potential applications in regenerative medicine.

Distribution, bioaccumulation, and trace element transfer among trophic levels in the southeastern Gulf of California

Our understanding of the trophic transfer of pollutants in marine subtropical ecosystems remains limited due to the complexity of their food webs. Thus, we aimed to evaluate Cd, Cu, Mn, Pb, and Zn sources, incorporation, and trophodynamics throughout the food web of the southeastern Gulf of California by stomach content analysis, stable isotope analysis, isotope mixing models, and trace element analysis in biological and environmental matrices. The food web comprised three main trophic guilds (TG1, TG2, and TG3). The bioaccumulation of Cd and Zn from seawater was efficient (> 1000) in TG2 and TG3. Bioaccumulation factor from sediment (BSAF >1) evidenced of Cd in all trophic guilds. In addition, non-trophic Cd relationships were identified in the food web. Based on the trophic magnification factor (TMF), Mn and Pb showed biodilution (TMFMn = 0.38; TMFPb = 0.16), while Cu and Zn exhibited biomagnification (TMFCu = 2.08; TMFZn = 3.31).

Trophic transfer of heavy metals through aquatic food web in the largest mangrove reserve of China

Understanding the mechanism of trophic transfer of heavy metal through the aquatic food web is critical to ecological exposure risk assessments in mangrove ecosystems. Zhanjiang Mangrove National Nature Reserve (ZMNNR) is the largest and biologically richest mangrove reserve in China, but has been exposed to heavy metal pollutants caused by the progressive industrialization and urbanization. We collected a variety of aquatic consumers, and primary producers, as well as sediments from the ZMNNR and analyzed them for heavy metal (Cd, Cr, Cu, Ni, Pb and Zn) concentrations, and for both δ13C and δ15N values to establish the trophic levels.The trophic magnification factors (TMF) of Cd, Cu and Zn are 0.19 (p < 0.01), 0.07 (p < 0.01) and 0.33 (p < 0.05), respectively, indicating significant biodilution in a simplified food web composed of bivalves, crustaceans and fish. There are also potential tendencies of biodilution for Cr, Ni and Pb. Comparison of heavy metals in representative fish and shrimp in the ZMNNR with those in worldwide mangroves indicate a low risk level for aquatic consumers in our ecosystem. Quantitative source tracking is conducted based on principal component analysis and cluster analysis, which indicate that Cr, Ni and Pb are mainly originated from natural geological processes, Cu and Zn from shrimp farming and agriculture activities, and Cd from the deposition of aerosol released by regional metal smelting industry.

Interplay between mesenchymal stromal cells and the immune system after transplantation: implications for advanced cell therapy in the retina.

Advanced mesenchymal stromal cell-based therapies for neurodegenerative diseases are widely investigated in preclinical models. Mesenchymal stromal cells are well positioned as therapeutics because they address the underlying mechanisms of neurodegeneration, namely trophic factor deprivation and neuroinflammation. Most studies have focused on the beneficial effects of mesenchymal stromal cell transplantation on neuronal survival or functional improvement. However, little attention has been paid to the interaction between mesenchymal stromal cells and the host immune system due to the immunomodulatory properties of mesenchymal stromal cells and the long-held belief of the immunoprivileged status of the central nervous system. Here, we review the crosstalk between mesenchymal stromal cells and the immune system in general and in the context of the central nervous system, focusing on recent work in the retina and the importance of the type of transplantation.