Growth Response Research Articles

Impact nano- and micro- form of CdO on barley growth and oxidative stress response

The objective was investigated the effects of CdO and nano-CdO as potential toxic pollutants on growth and redox response of barley. CdO and nano-CdO have been found to cause significant phytotoxicity in barley seedlings, with nano-CdO increasing plant tissue cadmium accumulation. This accumulation is linked to growth retardation and oxidative stress. Low molecular weight antioxidants like restored glutathione and ascorbate have been found to increase the activity of glutathione peroxidase (GPX), glutathione-S-transferase (GSTs), and ascorbate peroxidase (APX) in green tissues. Catalase (CAT) activity increased from 50 % with 100 mg/l CdO to 70 % with 1000 mg/l and nano-CdO. The observed disturbance in redox balance signals the upregulation of corresponding genes. Antioxidant enzyme isoform gene transcripts increased for SODB, CAT2, and APX. Cadmium buildup in root cells causes oxidative stress, leading to upregulation of SOD, CAT, GR, and GSTs isoform genes as well as protein carbonylation, sulfhydryl group degradation, and MDA accumulation. CdO and nano-CdO have similar phytotoxic effects, but bioavailability affects biochemical and molecular responses.

Optimization of iron, phosphate, and salinity in nutrient medium using response surface methodology for enhancing biochemical composition in Chlorella sp. culture

Microalga Chlorella sp. is a highly scientifically and commercially attractive unicellular microorganism and has developed a stable industry as a nutritional supplement for people and animals. Various nutrient media have been used to grow Chlorella sp. cultures to enhance their growth, pigmentation, and lipid content. However, the optimal biochemical composition and density of Chlorella sp. cultures require an understanding of optimizing the nutrient medium to study their production. The present study aims to investigate the effects of iron, phosphate, and salinity concentrations by working synergically in a nutrient medium on the growth responses, pigments, and lipid accumulation of Chlorella sp. culture using a response surface methodology (RSM) approach. Using the result from the RSM software, a total of 18 experimental groups (E1 – E18) were evaluated, and one confirmation (C) study was conducted. The results revealed that the E7 experiment in Chlorella sp. culture provided the highest cell density and specific growth rate (SGR) with 176.00 × 106 cells mL−1 and 0.35 day−1, respectively. Similarly, the E5 and E1 experiments produced the highest cell density of 166.10 × 106 cells. mL−1 and 152.13 × 106 cells mL−1, respectively. The SGR was also increased at 0.33 day−1 in the E5 experiment and 0.33 day−1 in the E1 experiment. Consequently, the culture of Chlorella sp. containing high iron and phosphate concentrations and lower salinity in a nutrient medium had the highest number of cells, SGR, and pigment accumulation (chlorophyll a and total carotenoid). In addition, the presence of high salinity concentrations reduces Chlorella sp. growth. However, the increase in the growth of Chlorella sp. culture did not indicate an increase in other biochemical compositions. In some cases, biochemical compositions are high due to nutritional limitations or stress factors. For example, in pigment accumulation, chlorophyll a pigment accumulation was increased in experiment E7 (51.57 μg mL−1), while total carotenoid accumulation was increased in experiment E8 (20.68 μg mL−1). In addition, increasing salinity concentration increased chlorophyll a and total carotenoid contents per cell, but decreased Chlorella sp. growth as shown in the E4 experiment, which achieved chlorophyll a levels of 1.38 pg cell−1 and total carotenoid levels of 0.52 pg cell−1. Furthermore, Chlorella sp. culture produces a higher lipid accumulation of 37.38 % in the E3 experiment. Hence, the results of this study contribute to understanding the optimal biochemical composition and cell growth of Chlorella sp. cultures.

Different responses of soil bacterial community to plant-plant interactions under organic-inorganic fertilizers affect seedling establishment during subalpine forest succession.

Rhizosphere bacterial community as a valuable indicator of soil quality and function, has been widespread studied. However, little knowledge is about the response of bacterial communities to plant-plant interaction and different fertilizers during secondary forest succession. We conducted a field pot experiment applying organic and inorganic fertilizers to monocultures and mixed cultures of dominant plant species from mid- to late-successional stages (Salix oritrepha, Betula albosinensis, and Picea asperata), and investigated the responses of plant growth and rhizosphere bacterial communities. Results indicated that growth rate of plant height varied among plant species, but no significant differences were observed in soil bacterial diversity and composition among plant species or inter-specific interactions under control. Compared to control, inorganic fertilizer resulted in increases in plant growth and the relative abundance of Proteobacteria, Patescibacteria, Bacteroidetes and Gemmatimonadetes, while simultaneously leading to decrease in the relative abundance of Acidobacteria, Actinobacteria, Chloroflexi, Rokubacteria and Planctomycetes. When grown with other species, the bacterial communities in the mixture resembled those of S. oritrepha in singular monoculture under inorganic fertilizer treatment, but plant growth was not affected by interspecific interaction. Unlike inorganic fertilizer, organic fertilizer significantly affected bacterial communities and increased bacterial diversity, but did not alter the effects of plant-plant interactions on bacterial communities. It was also observed that organic fertilizer facilitated later successional species' growth (P. asperata and B. albosinensis) by the mid-successional species (S. oritrepha), ultimately facilitating secondary forest succession. In addition, plants at different successional stages harbor specific bacterial communities to affect their growth, and the bacterial communities contributed more than soil properties to the variations in the plant growth of S. oritrepha and P. asperata though the bacterial communities were regulated by soil factors. This finding highlights the significance of the rhizosphere bacteria on plant growth and plant community succession. It also emphasize the importance of considering both plant-plant interactions and diverse fertilizer types in forest restoration efforts and provide valuable insights into optimizing agronomic practices for secondary forest succession.

Genome-Wide Identification, Phylogenetic, and Expression Analysis of Jasmonate ZIM-Domain Gene Family in Medicago Sativa L.

JASMONATE ZIM domain (JAZ) proteins, inhibitors of the jasmonic acid (JA) signaling pathway, are identified in different plants, such as rice and Arabidopsis. These proteins are crucial for growth, development, and abiotic stress responses. However, limited information is available regarding the JAZ family in alfalfa. This study identified 11 JAZ genes (MsJAZs) in the "Zhongmu No.1" reference genome of alfalfa. The physical and chemical properties, chromosome localization, phylogenetic relationships, gene structure, cis-acting elements, and collinearity of the 11 MsJAZ genes were subsequently analyzed. Tissue-specific analysis revealed distinct functions of different MsJAZ genes in growth and development. The expression patterns of MsJAZ genes under salt stress conditions were validated using qRT-PCR. All MsJAZ genes responded to salt stress, with varying levels of upregulation over time, highlighting their role in stress responses. Furthermore, heterogeneous expression of MsJAZ1 in Arabidopsis resulted in significantly lower seed germination and survival rates in OE-2 and OE-4 compared to the WT under 150 mM NaCl treatment. This study establishes a foundation for further exploration of the function of the JAZ family and provides significant insights into the genetic improvement of alfalfa.

Berberine Inhibits the Inflammatory Response Induced by Staphylococcus aureus Isolated from Atopic Eczema Patients via the TNF-α/Inflammation/RAGE Pathways.

Atopic eczema patients exhibit high levels of Staphylococcus aureus (S. aureus) skin colonization. S. aureus can stimulate macrophages and the expression of proinflammatory cytokines. Berberine (BBR), an alkaloid, attenuates S. aureus toxin production. This study investigated if BBR suppressed bacterial growth and inflammatory response induced by eczema-patient-derived S. aureus using murine macrophage (RAW 264.7) and human monocyte cell lines (U937). RAW 264.7 and U937 were treated with BBR at different concentrations and stimulated with heat-killed S. aureus (ATCC #33591) or S. aureus derived from severe eczema patients (EC01-EC10), who were undergoing topical steroid withdrawal, for 24 h. TNF-α protein levels were determined by ELISA, gene expression by qRT-PCR, cell cytotoxicity by trypan blue excursion, and reactive oxygen species (ROS) levels by fluorometric assay. BBR showed a bacteriostatic effect in S. aureus (ATCC strain #33591 and clinical isolates (EC01-EC10) and suppressed TNF-α production in RAW 264.7 and U937 cells exposed to heat-killed S. aureus (ATCC and clinical isolates) dose-dependently without any cell cytotoxicity. BBR (20 µg/mL) suppressed >90% of TNF-α production (p < 0.001), downregulated genes involved in inflammatory pathways, and inhibited S. aureus ROS production in U937 and RAW 264.7 cells (p < 0.01). BBR suppresses S. aureus-induced inflammation via inhibition of TNF-α release, ROS production, and expression of key genes involved in the inflammatory pathway.

Genome-wide identification of the grapevine β-1,3-glucanase gene (VviBG) family and expression analysis under different stresses.

The β-1,3-glucanase gene is widely involved in plant development and stress defense. However, an identification and expression analysis of the grape β-1,3-glucanase gene (VviBG) family had not been conducted prior to this study. Here, 42 VviBGs were identified in grapevine, all of which contain a GH-17 domain and a variable C-terminal domain. VviBGs were divided into three clades α, β and γ, and six subgroups A-F, with relatively conserved motifs/domains and intron/exon structures within each subgroup. The VviBG gene family contained four tandem repeat gene clusters. There were intra-species synteny relationships between two pairs of VviBGs and inter-species synteny relationships between 20 pairs of VviBGs and AtBGs. The VviBG promoter contained many cis-acting elements related to stress and hormone responses. Tissue-specific analysis showed that VviBGs exhibited distinct spatial and temporal expression patterns. Transcriptome analysis indicated that many VviBGs were induced by wounds, UV, downy mildew, cold, salt and drought, especially eight VviBGs in subgroup A of the γ clade. RT-qPCR analysis showed that these eight VviBGs were induced under abiotic stress (except for VviBG41 under cold stress), and most of them were induced at higher expression levels by PEG6000 and NaCl than under cold treatment. The chromosome localization, synteny and phylogenetic analysis of the VviBG members were first conducted. The cis-acting elements, transcriptome data and RT-qPCR analysis showed that VviBG genes play a crucial role in grape growth and stress (hormone, biotic and abiotic) responses. Our study laid a foundation for understanding their functions in grape resistance to different stresses.

Bovine papillomavirus gene expression and inflammatory pathway activation vary between equine sarcoid tumour subtypes

Equine sarcoids are common non-metastasising skin tumours in horses, associated with bovine papillomavirus (BPV) infection. Six subtypes are recognised (occult, verrucose, nodular, fibroblastic, mixed and malevolent lesions), with variable clinical behaviour. The pathophysiology underlying varying tumour phenotype is poorly understood, and previous data on associations with viral load have been conflicting. To better understand this clinical variation, we investigated associations between tumour subtype and viral load, viral early protein gene expression, and expression of 10 host genes by quantitative polymerase chain reaction in 27 sarcoids and 5 normal skin samples. Viral DNA copy number did not differ between subtypes but was significantly higher in animals with fewer tumours. Expression of BPV E2 and E6 was higher in occult lesions compared to fibroblastic or nodular lesions, while E5 expression was higher in previously-treated lesions. Of the host genes, only IL6 and IL1B differed between subtypes, with higher expression in fibroblastic lesions, while IL10 and CCL5 were elevated compared to skin in all lesion types, and elevations in TNF and TGFB1 were significant for occult lesions only. Expression of TLR9, ATR, VEGFA and PTGS2 in sarcoids was not significantly different from normal skin, suggesting differences between BPV and human papillomavirus tumorigenesis. Results for BPV viral load and gene expression differed from previous reports and are insufficient to explain the spectrum of tumour phenotypes. Activation of both pro-inflammatory and anti-inflammatory immune pathways in sarcoids could influence tumour growth and effective immune responses, and the contribution of specific infiltrating immune cells requires further investigation.

Characterizing metabolome signature of colostrum, transition and mature milk of indigenous cows (Bos indicus) adapted to high altitude environment of Leh-Ladakh

This study has identified 46 metabolites in colostrum, transition milk and mature milk of unique indigenous high altitude adapted Ladakhi cows using 1D 1H 800 MHz NMR spectroscopy. The multivariate analysis revealed that UDP-galactose, UDP-glucose, citrate, creatine phosphate, myo-inositol, lactose, 2-oxoglutarate, valine, maltose, leucine, dimethylamine, and choline with high VIP scores could differentiate the colostrum, transition and mature milk in separate clusters. . Highly enriched metabolites in colostrum such as UDP-galactose, UDP-glucose play crucial roles in cell growth, differentiation, and defense responses. Similarly, the presence of branched chain amino acids in colostrum could be linked to mammary gland development, N-acetylglucosamine, N-acetyl carnitine, choline etc. in high concentration in colostrum l might be helping in growth and development of neonatal calves of Ladakhi cows under hypoxia environment. Overall, this study has helped to characterize the metabolomic signatures of milk/colostrum of Ladakhi cows adapted to high altitude and cold desert of Leh-Ladakh.

Growth and physiology responses of Samanea saman inoculated with arbuscular mycorrhizal fungi in silica post-mining soil media using biodegradable pots

Arbuscular mycorrhizal fungi (AMF) and biodegradable pots are environmentally friendly and enhance plant growth in adverse soil conditions. These studies explored AMF interactions and biodegradable pots in physiology, growth, and the uptake of nutrients in Samanea saman seedlings. The present research interactive effects of biodegradable pots with different compositions and raw material sizes with and without AMF inoculation on S. saman grown in silica post-mining soil media. Results indicated AMF inoculations significantly improved leaf chlorophyll content, photosynthetic rate, heights, diameters, biomass, as well as nutrient absorption of S. saman as compared with non-inoculated plants in biodegradable pots. AMF and biodegradable pots composed of 15% used newspaper, 70% compost, 5% cocopeat, and 10% rock phosphate showed the best results and increased the leaf chlorophyll content, photosynthesis rate, height, diameter, and total biomass of the plants by 161.1%, 208.7%, 263.6%, 118.1%, and 269.9%, respectively, compared to biodegradable pots composed only of used newspaper. Additionally, uptake of the nutrients nitrogen, phosphate, potassium, and magnesium increased by 365%, 800%, 369%, and 250%, respectively. By the fourteenth week after transplanting, the C/N ratio of the organic pot decreased significantly. Thus, AMF and biodegradable pots containing compost and rock phosphate interact positively and enhance the growth of S. saman under adverse soil conditions. These findings suggest that biodegradable pots containing compost and rock phosphate show potential as more environmentally friendly replacements for plastic bags (polybags) as growth medium containers for seedling production.

Explore the Role of the Sphingosine-1-Phosphate Signalling as a Novel Promising Therapeutic Target for the Management of Parkinson's Disease.

Sphingosine-1-phosphate (S1P) is a cellular signalling molecule derived from sphingosine, which is a pro-apoptotic sphingolipid. Sphingolipids control various cellular actions like growth, homeostasis, and stress-related responses. The main sources of S1P in our body are erythrocytes. S1P controls both cellular mediators and other second messengers intracellularly. The S1P receptor also helps in inflammatory and neuroprotective effects (required to manage of Parkinson's). A large number of anti-Parkinson drugs are available, but still, there is a need for more effective and safer drugs. S1P and its receptors could be targeted as novel drugs due to their involvement in neuro-inflammation and Parkinson's. The present review effort to explore the biological role of S1P and related receptors, for their possible involvement in PD; furthermore. Overall, S1P and other related metabolizing enzymes have significant therapeutic opportunities for Parkinson's disease along with other neurological disorders.

SlCHP16 promotes root growth and enhances saline-alkali tolerance of tomato

With the increasing salinization in the world, crop growth and yield had a serious threat. Roots as the organ of the plant direct contact the saline-alkali environment has received more and more attention. The Divergent C1 (DC1) domain protein plays an important role in plant growth, development, and stress response. In this study, overexpression of SlCHP16 promoted tomato root growth, while knocking out SlCHP16 inhibited tomato root growth. In the roots of SlCHP16 overexpressing plants, the auxin synthesis key gene SlTAA2 was significantly up-regulated, which increased auxin synthesis and accumulation and promoted root cell elongation. Meanwhile, the expression levels of cell expanding-related genes SlLRP, SlXTH9 and SlEXPB1 were up-regulated. The opposite was observed in SlCHP16 knockout plants. Under saline-alkali stress, the root growth rate of SlCHP16 overexpressed lines was significantly higher than that of AC, and SlCHP16 knockout lines had poor root development during seed germination and seedling growth. At the same time, after saline-alkali stress treatment, SlCHP16-overexpressing lines showed higher tolerance, while SlCHP16 knockout plants were more sensitive to saline-alkali stress. In conclusion, SlCHP16 promoted root growth and enhanced saline-alkali tolerance in tomato. This work provides new insights into the mechanism of tomato root development and provides resources for developing new salt-alkali tolerant tomato varieties.

Comparison of the Effects of Hesperidin Over 30- and 60-Day Intervals on Rainbow Trout: A Potential Biostimulant to Promote Growth, Immunological and Antioxidant Responses, and Disease Resistance

Abstract The current research evaluated the effects of dietary hesperidin (HSP) on growth parameters, digestive enzyme activities, innate immune markers, and antioxidant responses in rainbow trout, Oncorhynchus mykiss after the 30- and 60-day feeding trial. Then, specimens were subjected to Yersinia ruckeri infection for 14 days. For this, six hundred rainbow trout juveniles (initial weight; 25.49±0.40 g) were fed with different levels of HSP including 0 (HSP0; control), 50 (HSP50), 100 (HSP100), 150 (HSP150), and 200 (HSP200) mg/kg feed. After 60 days, dietary HSP100 supplementation significantly improved growth and feed efficiency indices. The optimal dose of HSP based on the regression test for WG and FCR detected 122 and 131 mg/kg, respectively. At the end of the 30th day, dietary HSP150 supplementation markedly boosted serum lysozyme (LYZ), myeloperoxidase (MPO), complement component C3, and immunoglobulin (Ig) levels, but decreased malondialdehyde (MDA) content. In the same period, dietary administration of HSP at the different concentrations markedly increased complement component C4 (HSP150 and HSP200), superoxide dismutase (SOD), skin mucus alkaline phosphatase (ALP) (HSP100–HSP200), skin mucus LYZ and catalase (CAT) (HSP100 and HSP 150), and skin mucus Ig level (HSP50–HSP200). After 60 days, all dietary HSP supplementation significantly improved lipase, serum C3, glutathione peroxidase (GPX), and skin mucus ALP, LYZ, and Ig levels, but decreased MDA, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) values. After 60 days, dietary administration of HSP150 induced a significant discrepancy in the activity of serum LYZ, MPO, respiratory burst (RB), and lactate dehydrogenase (LDH) compared to the control group. In the same period, dietary HSP supplementation at different levels induced a remarkable increase in digestive protease activity (HSP100), serum ACH50 and CAT activities (HSP100 and HSP150), skin mucus peroxidase and serum SOD levels (HSP100–HSP200), and serum C4 value (HSP50, HSP150, and HSP200), but markedly decreased ALP activity (HSP150 and HSP200). In addition, dietary HSP supplementation, especially HSP150, markedly boosted disease resistance against Y. ruckeri infection. The current data demonstrated that the oral administration of 100–150 mg/kg HSP has a high potential to promote growth performance, disease resistance, and faster induction of immune and antioxidant responses.

PavSPLs are key regulators of growth, development, and stress response in sweet cherry

SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes are plant-specific transcription factors essential for plant growth, development, and stress responses; however, their roles in sweet cherry are not well understood. In this study, we identified and isolated 16 SPL genes from the sweet cherry genome, categorizing them into five subfamilies, with 12 PavSPLs predicted as miR156 targets. The promoter regions of PavSPLs contain cis-elements associated with light, stress, and phytohormone responses, indicating their role in biological processes and abiotic stress responses. Seasonal expression analysis showed that PavSPL regulates sweet cherry recovery after dormancy. Gibberellin (GA) treatment reduced PavSPL expression, indicating its role in GA-mediated processes. Overexpression of PavSPL14 in Arabidopsis thaliana resulted in earlier flowering, increased plant height, and enhanced growth. Yeast two-hybrid assays revealed an interaction between PavSPL14 and DELLA protein PavDWARF8, suggesting the involvement of PavSPLs in GA-regulated processes through protein interactions. These findings lay the groundwork for future studies on PavSPL function in sweet cherry.

Green synthesis of Z-scheme CeO2 decorated ZnO nanocomposites for photodegradation of organic pollutants, antioxidant activity and its effects on seed germination

The present work aimed to report a green synthesis of CeO2@ZnO nanocomposites via co-precipitation method using azadirachta indica aqueous leaf extract. The characterization of the prepared CeO2@ZnO nanocomposites were done using XRD, FTIR, SEM, EDAX, UV–vis, and PL techniques to examine the structural properties, functional group, morphological and electronic properties. As prepared CeO2@ZnO nanocomposites have been used for photo degradation of Crystal Violet (CV), Methylene blue (MB) and Rhodamine B (RhB) dyes in presence of solar light in aqueous solution. From structural analysis the mean crystallite sizes of the synthesized nanocomposites varied from 27 nm to 9 nm. The degradation of MB, CV and RhB dyes follows pseudo-first order reaction rate with the rate constantsk1 for MB is 10.09 × 10−3 min−1, k1 for CV is 8.71 × 10−3 min−1, k1 for RhB is 0.92 × 10−3 min−1.The characterized nanocomposites were used for seedling growth, physiological and biochemical responses during seed germination. In CeO2@ZnO nanocomposites treatments, significantly higher values of shoot length and root length were observed in CZ1 (0.5, and 1 mg/ml) treated seedlings as compared to other samples. Similarly, the activities of ɑ-amylase and protease enzymes were recorded to be maximum for CZ1 nanocomposites. For instance, the increased activity of α-amylase was observed to be 2.12, 2.09, and 1.33 folds higher in CZ1, CZ2, and CZ3 (1 mg/ml) treated seedlings as compared to the control. Similarly, the protease activity was reported to be increased by 0.011 folds in CZ1 nanocomposites (1 mg/ml) treated seedlings as compared to the control. Furthermore, all the CeO2@ZnOnanocomposites have excellent DPPH, and ABTS free radical scavenging activities, confirmed by the Ferric-Reducing Antioxidant Power (FRAP) assay. The CZ1 nanocomposites showed the best results. The CeO2@ZnO nanocomposites were found to have no toxic effects on cellular division confirmed with improved mitotic index under different treatment regimens.

Improved growth performance and physiological state in hybrid abalone (Haliotis rufescens and Haliotis fulgens) facing ocean acidification conditions

Abalone (Haliotis spp.) is a valuable global aquaculture resource in Mexican abalone industry, particularly red (Haliotis rufescens) and green (H. fulgens) abalone, showing significant growth. However, climate change -particularly ocean acidification (OA)- threatens production by negatively impacting mollusks, leading to recruitment failures, poor fertilization, malformations, and reduced calcification and growth jeopardizing sustainability and food security. This study aims to assess robustness of a hybrid abalone cross RF (H. rufescens ♀ x H. fulgens ♂) to OA conditions by evaluating biological performance (survival, growth in weight and length) and metabolic response (oxygen consumption, ammonia excretion rate, and atomic O:N ratio). Juveniles from hybrid RF cross and two pure parental crosses (RR, FF) were exposed to three pH levels: control (pH ≈ 8.0) and two reduced pH scenarios (pH 7.8 and 7.5), representing moderate and severe OA conditions. The results showed hybrid cross exhibiting better growth and metabolic response than pure crosses under all conditions. Notably, hybrid length growth was superior under moderate OA conditions (pH 7.8) compared to control (pH 8.0) and intense OA conditions (pH 7.5). Enhanced physiological hybrid status was evidenced by increased oxygen consumption and stable ammonia excretion, reflected in higher atomic O:N ratio. This study demonstrates hybrid cross RF robustness under OA conditions and supports interspecific hybridization as potential mitigation strategy to alleviate negative OA effects for sustainable abalone production.

Resilience of Juvenile Freshwater Pearl Mussels to ThermalStress.

Climate change poses a significant threat to freshwater ecosystems by causing increases of average water temperatures, and more frequent and extreme heating events. Freshwater mussels are declining globally, and the distribution of the freshwater pearl mussel (Margeritifera margeritifera) has decreased dramatically over the past century. Even though it is likely that climate change is contributing to the decline of the species, little is known about the specific mechanisms involved. Here, we test how short episodes of water temperatures above the known thermotolerance range affect the survival and growth of the early post parasitic juvenile phase of freshwater pearl mussels. We also test if previous experience with elevated water temperatures can modify survival and growth responses to subsequent high-temperatures exposure. Mortality was very low in all treatments (< 5%) and not affected by the temperature treatments, while growth rate was positively affected by temperature. Our results suggest that juvenile mussels can survive short periods of heat stress when other environmental conditions are favourable. Future studies should therefore address how heat stress affects survival in combination with other stressors, such as reduced availability of dissolved oxygen.

TLR2–EGR1 signaling axis modulates TGFβ1-induced differentiation of fibroblasts into myofibroblasts in pulmonary fibrosis

Pulmonary fibrosis is a progressive lung condition characterized by the excessive activation of myofibroblasts. Transforming growth factor beta 1 (TGFβ1) plays a crucial role in the differentiation of fibroblasts into myofibroblasts. In addition, toll-like receptor 2 (TLR2), known for its role in immune responses, contributes to pulmonary fibrosis by promoting myofibroblast differentiation. However, the interplay between TGFβ1 and TLR2 signaling pathways in myofibroblast differentiation has remained elusive. In the present study, we investigated the involvement of TLR2 in TGFβ1-induced fibroblast differentiation into myofibroblasts using IMR-90 human pulmonary fibroblasts as a model cell line. We found that TLR2 activation induced myofibroblast differentiation by enhancing the expression of early growth response 1 (EGR1) via the mitogen-activated protein kinase (MAPK) signaling pathway. Elevated EGR1 levels were detected in the lung tissues of a bleomycin (BLM)-induced mouse model of pulmonary fibrosis. Moreover, the administration of tomaralimab, an antagonistic anti-TLR2 antibody, reduced the EGR1 expression and collagen deposition. Altogether, targeting the TLR2–EGR1 pathway could be a promising therapeutic approach for pulmonary fibrosis by blocking TGFβ1-induced myofibroblast differentiation.

High expression of transcription factor EGR1 is associated with postoperative muscle atrophy in patients with knee osteoarthritis undergoing total knee arthroplasty

BackgroundMuscle atrophy is a typical affliction in patients affected by knee Osteoarthritis (KOA). This study aimed to examine the potential pathogenesis and biomarkers that coalesce to induce muscle atrophy, primarily through the utilization of bioinformatics analysis.MethodsTwo distinct public datasets of osteoarthritis and muscle atrophy (GSE82107 and GSE205431) were subjected to differential gene expression analysis and gene set enrichment analysis (GSEA) to probe for common differentially expressed genes (DEGs) and conduct transcription factor (TF) enrichment analysis from such genes. Venn diagrams were used to identify the target TF, followed by the construction of a protein-protein interaction (PPI) network of the common DEGs governed by the target TF. Hub genes were determined through the CytoHubba plug-in whilst their biological functions were assessed using GSEA analysis in the GTEx database. To validate the study, reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR), enzyme-linked immunosorbent assay (ELISA), and Flow Cytometry techniques were employed.ResultsA total of 138 common DEGs of osteoarthritis and muscle atrophy were identified, with 16 TFs exhibiting notable expression patterns in both datasets. Venn diagram analysis identified early growth response gene-1 (EGR1) as the target TF, enriched in critical pathways such as epithelial mesenchymal transition, tumor necrosis factor-alpha signaling NF-κB, and inflammatory response. PPI analysis revealed five hub genes, including EGR1, FOS, FOSB, KLF2, and JUNB. The reliability of EGR1 was confirmed by validation testing, corroborating bioinformatics analysis trends.ConclusionsEGR1, FOS, FOSB, KLF2, and JUNB are intricately involved in muscle atrophy development. High EGR1 expression directly regulated these hub genes, significantly influencing postoperative muscle atrophy progression in KOA patients.

What determines transfer of carbon from plants to mycorrhizal fungi?

Biological Market Models are common evolutionary frameworks to understand the maintenance of mutualism in mycorrhizas. 'Surplus C' hypotheses provide an alternative framework where stoichiometry and source-sink dynamics govern mycorrhizal function. A critical difference between these frameworks is whether carbon transfer from plants is regulated by nutrient transfer from fungi or through source-sink dynamics. In this review, we: provide a historical perspective; summarize studies that asked whether plants transfer more carbon to fungi that transfer more nutrients; conduct a meta-analysis to assess whether mycorrhizal plant growth suppressions are related to carbon transfer; and review literature on cellular mechanisms for carbon transfer. In sum, current knowledge does not indicate that carbon transfer from plants is directly regulated by nutrient delivery from fungi. Further, mycorrhizal plant growth responses were linked to nutrient uptake rather than carbon transfer. These findings are more consistent with 'Surplus C' hypotheses than Biological Market Models. However, we also identify research gaps, and future research may uncover a mechanism directly linking carbon and nutrient transfer. Until then, we urge caution when applying economic terminology to describe mycorrhizas. We present a synthesis of ideas, consider knowledge gaps, and suggest experiments to advance the field.

Tangeretin inhibits airway inflammatory responses by reducing early growth response 1 (EGR1) expression in mice exposed to cigarette smoke and lipopolysaccharide

BackgroundTangeretin, a natural polymethoxyflavone compound, possesses potent anti-inflammatory activity that improves respiratory inflammation in chronic obstructive pulmonary disease (COPD). However, the molecular mechanisms underlying the anti-COPD effects of tangeretin remain unclear. In this study, we aimed to investigate the key molecular mechanisms by which tangeretin suppresses COPD-related inflammatory responses. MethodsWe conducted the investigation in phorbol-12-myristate-13-acetate (PMA)-stimulated human airway epithelial cells (in vitro) and cigarette smoke (CS)/lipopolysaccharide (LPS)-exposed mice (in vivo). ResultsTangeretin decreased the release of inflammatory mediators, including tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and mucin 5AC (MUC5AC), by suppressing early growth response 1 (EGR1) expression in vitro. Tangeretin and EGR1 small interfering ribonucleic acid (siRNA) combination showed a synergistic reduction in MUC5AC and TNF-α secretion. Tangeretin administration significantly inhibited the levels of reactive oxygen species (ROS) production, elastase activity, TNF-α, IL-6, and monocyte chemoattractant protein-1 (MCP-1) secretion, and macrophage and neutrophil numbers in the bronchoalveolar lavage fluid of CS/LPS-exposed mice. Tangeretin also prevented CS/LPS-induced abnormal pathological changes and excessive MUC5AC and EGR1 expression in lung tissue. ConclusionComprehensively, tangeretin inhibits the lung inflammatory response associated with COPD by reducing EGR1 expression in PMA-induced human epithelial cells and in a CS/LPS-stimulated mouse model. This study shows that tangeretin has anti-COPD properties and can be a promising alternative (or complementary) treatment for inflammatory lung disease.