Growth Response Research Articles

Genome-wide analysis of the WOX gene family and function exploration of RhWOX331 in rose (R. 'The Fairy').

WOXs are a class of plant-specific transcription factors that play key roles in plant growth and stress responses. However, the mechanism by which WOXs influence adventitious root development in Rosa hybrida remains unclear. In this study, RcWOX gene family in rose was identified and phylogenetically analyzed using bioinformatics analysis. A total of 381 RcWOX gene members were localized on seven chromosomes except of nine members. The main cis-acting elements involved in hormonal, light, developmental, and abiotic stress responses were identified in the promoters of RcWOX genes, suggesting their regulation by these signals. Nine RhWOX genes had significant different expression during rooting process of rose. RhWOX331, RhWOX308, RhWOX318 were positive with the formation of rose roots. RhWOX331 was positively involved in the formation of adventitious root primordia, which gene coding a transcription factor localized in the nucleus. The HOX conserved domain in the protein contributed to the self-activating activity of RhWOX331. We obtained genetically modified Arabidopsis to validate the function of RhWOX331. Overexpression of RhWOX331 gene alleviated the inhibition of root length of A. thaliana primary roots by high concentration of IBA and NPA, and significantly increased the number of lateral roots on the primary roots, as well as the height of A. thaliana plants. Additionally, RhWOX331 promoted adventitious root formation in A. thaliana and mitigated hormonal inhibition by exogenous 6-BA, NPA, and GA3. The RhWOX331 promoter contained cis-acting elements such as ABRE, Box 4 and CGTCA-motif et.al. GUS activity analysis showed that the gene acted at the cotyledon attachment site. Taken together, these studies identified a significant expansion of the RcWOX gene family, inferred roles of certain branch members in adventitious root formation, elucidated the function of RhWOX331 in adventitious root initiation, and laid the foundation for further research on the function of WOX gene family in roses.

Public and Private Investment as Catalysts for Growth: An analysis of emerging markets and developing economies with a focus on Asia

The paper analyzes the growth impact of public and private investment shocks based on a large sample of emerging and developing countries over the period 1980–2021 with a particular focus on the Asian region. We develop new measures of investment shocks based on cyclically adjusted investment data. Estimations using local projections suggest that public investment shocks play a much greater role in boosting economic growth in comparison with private investment shocks. In EMDEs (including in Asia) the growth response to investment shocks is positive and much stronger in recessions (relative to economic expansions) and in countries with more fiscal space. Finally, public investment shocks in EMDE and Asian samples crowd-in private investment and private consumption.

Acceleration of the Banking Sector and Economic Growth: Effectiveness of Economic Recovery Strategies Post-Covid-19

The banking sector around the world is under great pressure due to the current state of the Covid-19. This study investigates how Covid-19 impacts systemic risk in the banking sector across all types of banking lines affected by Covid-19. This study found that all banking lines experienced a significant increase in systemic risk among the types of banking that were sampled initially. By using spillover policies, it is also possible to identify institutions that are systemically important. The analysis was performed using Panel Data Regression, SVAR, and FEVD on individual bank data and macroeconomic data. The findings proved that government policies to drive the business cycle and MSME actors in the context of overcoming the impact of Covid-19, have not shown their effectiveness. This is indicated by the negative response of economic growth to the policies that have been issued. On the other hand, the performance of banking financial institutions also contributes to national economic growth. This is indicated by the sensitivity of the bank's internal variables in responding to national economic growth. Likewise, with the response to government policies and macroeconomic conditions. Therefore, there is a need for an acceleration policy in handling the current national economic recovery.Originality/Value: This paper focus on systematic risk in banking during COVID-19, use of advanced econometric techniques, evaluation of government policies, integration of bank performance and macroeconomic growth, and its assessment of policy effectiveness, and its implications for future economic recovery efforts.

Effects of dietary immunostimulants on the growth and non-specific immune responses of hybrid sturgeons (Huso huso♀ × Acipenser ruthenus♂)

Herein, we investigated the effects of immunostimulants on the growth, immune responses, and post-infection survival of hybrid sturgeons (Huso huso♀ × Acipenser ruthenus♂). Therefore, we examined the effects of dietary supplementation of β-glucan, vitamin C, and Radix Bupleuri (Chaihu) extract (RBE) at various concentrations (0.2, 0.4, and 0.6% incorporated through feed) on the growth rate and non-specific immune responses of hybrid sturgeon. When supplemented individually, these immunostimulants significantly reduced the feed conversion ratio while promoting weight gain, with the vitamin C-administered groups showing the most pronounced differences. Further, we observed that dietary β-glucan, vitamin C, and RBE, when fed at 0.4 and 0.6% concentrations, significantly enhanced the rates of superoxide anion production and phagocytosis by head kidney leukocytes. Moreover, vitamin C and RBE supplementation at 0.4 and 0.6% and β-glucan supplementation at 0.6% concentration significantly increased the serum lysozyme activity. Further, vitamin C and β-glucan supplementation at 0.6% and RBE supplementation at 0.4 and 0.6% concentrations showed the highest survival rates after Aeromonas hydrophila infection. In conclusion, our findings highlight that the dietary supplementation of β-glucan, vitamin C, or RBE in appropriate concentrations can significantly enhance superoxide anion production and phagocytic activity of leukocytes, serum lysozyme activity, and the survival of hybrid sturgeons after A. hydrophila infection. Our results suggest that incorporating immunostimulants such as Chinese medicinal herbs at optimal levels can effectively boost the growth, immunity, and disease resistance of hybrid sturgeons in aquaculture. These results provide valuable insights for future studies on the inclusion of immunostimulants in aquaculture feed.

Fusobacterium nucleatum induces invasive growth and angiogenic responses in malignant oral keratinocytes that are cell line- and bacterial strain-specific.

Fusobacterium nucleatum is an anaerobic commensal of the oral cavity recently reported to be associated with cancers of the gastrointestinal tract and oral squamous cell carcinoma (OSCC). In this study, we investigate the impact on oral keratinocytes of infection with a genetically diverse set of strains of F. nucleatum subsp. polymorphum recovered from patients with oral dysplasia (n=6). We employed H357 oral keratinocytes derived from a stage 1 OSCC and H376 cells derived from a stage 3 OSCC. Adhesion phenotypes were strain specific, with 3/6 clinical isolates examined exhibiting higher adherence to the stage 3 H376 cell line. Conversely, intracellular invasion was greatest in the H357 cells and was associated with specific transcriptional responses including autophagy and keratinization. Infection of both H357 and H376 cell lines induced transcriptional and cytokine responses linked to cancer cell migration and angiogenesis. F. nucleatum infection induced greater levels of MMP9 secretion in the H376 cell line which was associated with enhanced motility and invasion phenotypes. Additionally, the degree of F. nucleatum induced invasive growth by H376 cells varied between different clinical isolates of F. nucleatum subsp. polymorphum. Blockage of CCL5 signalling using the inhibitor metCCL5 resulted in reduced keratinocyte invasion. F. nucleatum infection also induced expression of the pro-angiogenic chemokine MCP-1 and the angiogenic growth factor VEGF-A resulting in increased capillary-like tube formation in HUVEC cells, most significantly in H376 cells. Treatment of HUVEC cells with resveratrol, a VEGF-A signalling inhibitor, significantly attenuated F. nucleatum induced tube formation. Our data indicate that the outcomes of F. nucleatum-oral cell interactions can vary greatly depending on the bacterial genotype and the malignant phenotype of the host cell.

Interorgan, intraorgan and interplant communication mediated by nitric oxide and related species.

Plant survival to a potential plethora of diverse environmental insults is underpinned by coordinated communication amongst organs to help shape effective responses to these environmental challenges at the whole plant level. This interorgan communication is supported by a complex signal network that regulates growth, development and environmental responses. Nitric oxide (NO) has emerged as a key signalling molecule in plants. However, its potential role in interorgan communication has only recently started to come into view. Direct and indirect evidence has emerged supporting that NO and related species (S-nitrosoglutathione, nitro-linolenic acid) are mobile interorgan signals transmitting responses to stresses such as hypoxia and heat. Beyond their role as mobile signals, NO and related species are involved in mediating xylem development, thus contributing to efficient root-shoot communication. Moreover, NO and related species are regulators in intraorgan systemic defence responses aiming an effective, coordinated defence against pathogens. Beyond its in planta signalling role, NO and related species may act as ex planta signals coordinating external leaf-to-leaf, root-to-leaf but also plant-to-plant communication. Here, we discuss these exciting developments and emphasise how their manipulation may provide novel strategies for crop improvement.

Scaling up the Natural Mode of Action of Macrophyte Allelochemicals and Their Effect on Toxic Cyanobacteria Using a Nitrogen-Limited Chemostat

This study investigates the inhibitory and hormetic effects of Myriophyllum spicatum extract on Microcystis aeruginosa in a controlled, continuous culture environment. To address the global challenge posed by harmful algal blooms, we used a range of extract concentrations to delineate the growth response patterns. At very low concentrations (6.25 and 12.5 mg/L), the addition of M. spicatum extract shows no discernible reduction in M. aeruginosa cell density or growth rate; instead, a slight increase is observed during exposure, suggesting a hormetic effect. However, at higher concentrations (75 and 100 mg/L), there is a drastic reduction of more than 50% in cell density and growth rate at 75 mg/L, with complete inhibition at 100 mg/L, leading to pronounced oxidative stress, damage to antioxidant defense systems, and increased cell mortality. Increased levels of malondialdehyde, catalase, and superoxide dismutase activities indicate the involvement of these enzymes in combating oxidative stress. Furthermore, intracellular and extracellular microcystins were significantly decreased at higher extract concentrations (50, 75, and 100 mg/L) in a dose-dependent manner. Our results indicate a dose-dependent response and provide insight into the potential application of natural water treatment solutions. Implications for ecological management and future research directions are discussed.

Microbial resistance and resilience to drought across a European climate gradient

Drought and rainfall events will become more frequent and intense with climate change. At the same time, soil moisture is one of the major factors controlling soil microbial processes such as carbon cycling. When challenged with drought there are two main growth responses microorganisms can use: (1) they can maintain growth rates during drought (i.e., resistance) and (2) they can recover growth rates faster when the drought ends (i.e., resilience). Microbial communities are shaped by multiple other factors in the soil environment, however how those impact drought responses remain unclear. Here we investigate how climate (estimated as aridity index) and soil properties determine microbial growth resistance and resilience to drought across a climate gradient in Europe. To test this, we exposed the different soils to a standardised drought cycle in controlled conditions. We assessed bacterial growth, fungal growth and respiration during soil drying to determine resistance and in high resolution during three days after rewetting to estimate resilience to drought. We found that alpha diversity was the strongest driver of both bacterial drought resistance and resilience, which occurred via changes in soil pH. This shows the importance of diversity for sustaining bacterial functions during drought stress. A secondary driver of bacterial drought resistance and resilience was the aridity index was also an important driver, where bacterial communities from more arid climates had higher resistance and resilience to drought. Fungal communities were both more resistant and resilient compared to bacteria, but this was independent of other measured environmental factors. Bacterial resilience was partly linked with differences in community composition. Our results suggest that if sites are exposed to increased aridity due to climate change or are managed to promote bacterial diversity, they will have higher bacterial growth rates during drought perturbations, which could potentially promote soil carbon storage.

Schwann cell-derived exosomes ameliorate peripheral neuropathy induced by ablation of dicer in Schwann cells.

MicroRNAs (miRNAs) in Schwann cells (SCs) mediate peripheral nerve function. Ablating Dicer, a key gene in miRNA biogenesis, in SCs causes peripheral neuropathy. Exosomes from healthy SCs (SC-Exo) ameliorate diabetic peripheral neuropathy in part via miRNAs. Thus, using transgenic mice with conditional and inducible ablation of Dicer in proteolipid protein (PLP) expressing SCs (PLP-cKO), we examined whether SC-Exo could reduce peripheral neuropathy in PLP-cKO mice. PLP-cKO mice at the age of 16 weeks (8 week post-Tamoxifen) were randomly treated with SC-Exo or saline weekly for 8 weeks. Age-and sex-matched wild-type (WT) littermates were used as controls. Peripheral neurological functions, sciatic nerve integrity, and myelination were analyzed. Quantitative RT-PCR and Western blot analyses were performed to examine miRNA and protein expression in sciatic nerve tissues, respectively. Compared to the WT mice, PLP-cKO mice exhibited a significant decrease in motor and sensory conduction velocities, thermal sensitivity, and motor coordination. PLP-cKO mice exhibited substantial demyelination and axonal damage of the sciatic nerve. Treatment of PLP-cKO mice with SC-Exo significantly ameliorated the peripheral neuropathy and sciatic nerve damage. PLP-cKO mice showed a substantial reduction in a set of Dicer-related miRNAs known to regulate myelination, axonal integrity, and inflammation such as miR-138, -146a and - 338 in the sciatic nerve. In addition, PLP-cKO mice exhibited significant reduction of myelin forming proteins, early growth response 2 (EGR2) and sex determining region Y-box10 (Sox10), but significantly increased myelination inhibitors, Notch1, c-Jun, and Sox2 and the axonal growth inhibitor phosphatase and tens in homolog (PTEN). However, SC-Exo treatment reversed the PLP-cKO altered miRNAs and proteins. This study demonstrates that exogenous SC-Exo ameliorate peripheral neuropathy induced by Dicer ablation in PLP expressing SCs. The therapeutic benefit may be mediated by the SC-Exo altered miRNAs and their targeted genes.

Pure and mixed Scots pine forests showed divergent responses to climate variation and increased intrinsic water use efficiency across a European-wide climate gradient

AbstractThe present study examined Pinus sylvestris L. growth responses to climatic variations and its relationship with intrinsic water-use efficiency (iWUE) across a water availability gradient and also in pure P. sylvestris and P. sylvestris-Quercus species mixed forests. Study sites were selected in the Mediterranean, temperate, and temperate continental climates in Spain, Italy, and Poland, respectively. A combined tree-ring dendrochronological and stable carbon isotope analysis was used to assess the relationship between tree growth and climate variation. Results showed that P. sylvestris growth is critically affected by summer water availability, regardless of study site and species mixing. Warming temperatures during the early growing season benefit tree growth in Mediterranean and temperate continental climates, while no significant effect was observed in the temperate climatic conditions. At the Mediterranean site, trees in mixed stands showed enhanced growth during wet years when moisture is not limiting. At the temperate continental site, trees in the mixed stand grew at a lower rate than those in pure stands, which suggests that intense interspecific competition for water could overwhelm the benefits of species mixing. Also, we found a divergent growth-iWUE relationship of non-significant and significantly positive and significantly negative correlations at the Polish, Italian, and Spanish sites, respectively. Overall, the negative growth-iWUE relationship at the drier Mediterranean site signifies the risk of tree growth decline, particularly in drier climate conditions. Despite that, elevated iWUE levels would benefit tree radial growth when water is not limited and the admixing tree species have compatible light and water use strategies.

Impact of Chaetomorpha aerea-enriched diet on growth, feed utilization, and haemato-immunological responses in Clarias batrachus challenged with Aeromonas hydrophila

The bacteria Aeromonas hydrophila, which causes motile Aeromonas septicemia (MAS), is dangerous to aquaculture because it affects the fish's well-being and production. As the aquaculture industry seeks sustainable and effective methods to enhance fish immunity and growth, natural supplements such as marine algae have gained attention. This study explored the potential benefits of incorporating the green marine algae Chaetomorpha aerea into the fish diet, focusing on disease resistance, growth, feed utilization, and hematological and immunological responses. Five diets were prepared, varying concentrations of C. aerea (0 control, T1: 1 g/kg; T2: 2 g/kg: T3: 5 g/kg: and T4: 10 g/kg) and administered to fish over 30 days. Following the feeding trial, the fish were exposed to A. hydrophila, and their survival rates were observed for the next 14 days. The findings demonstrated that the final weight, weight gain, relative growth rate, specific growth rate, and daily growth rate were all positively impacted by a diet containing 5 g/kg of C. aerea. Additionally, fish in the 5 g/kg C. aerea group demonstrated improved feed conversion efficiency compared to the control group. While there were no significant changes in red and white blood cell counts on the initial day, serum lysozyme activity and overall resistance to infection were enhanced in fish receiving C. aerea at 2 and 5 g/kg. These results imply that C. aerea supplementation with fish supplements may be a useful immunostimulant, boosting improved health and growth in sustainable aquaculture practices.

The crosstalk interaction of ethylene, gibberellins, and arbuscular mycorrhiza improves growth in salinized tomato plants by modulating the hormonal balance

Ethylene (ET) and gibberellins (GAs) play key roles in controlling the biotic and abiotic interactions between plants and environment. To gain insights about the role of ET and GAs interactions in the mycorrization and response to salinity of tomato (Solanum lycopersicum L.) plants, the ET-insensitive (Never-ripe, Nr), and the ET-overproducer (Epinastic, Epi) mutants and their wild type cv. Micro-Tom (MT), were inoculated or not with the arbuscular mycorrhizal fungi (AMF) Rhizophagus irregularis and exposed to control (0 mM NaCl) and salinity (100 mM NaCl) conditions, with and without gibberellic acid (10−6 M GA3) application during four weeks. Exogenous GA3 enhanced plant growth irrespective of the genotype, AMF, and salinity conditions, while an additional effect on growth by AMF was only found in the ET-overproducer (Epi) mutant under control and salinity conditions. Epi almost doubled the AMF colonization level under both conditions but was the most affected by salinity and GA3 application compared to MT and Nr. In contrast, Nr registered the lowest AMF colonization level, but GA3 produced a positive effect, particularly under salinity, with the highest leaf growth recovery. Foliar GA3 application increased the endogenous concentration of GA1, GA3, and total GAs, more intensively in AMF-Epi plants, where induced levels of the ET-precursor ACC were also found. Interestingly, GA4 which is associated with AMF colonization, registered the strongest genotype x GA x AMF × salinity interactions. The different growth responses in relation to those interactions are discussed.

Effect of Replacement of Soybean Meal with Fermented Rice Bran as Protein Source on Growth Response, Feeding Utilization, Body Composition, Intestinal Microbiota and an Economic Evaluation of the Grey mullet, Mugil cephalus

Effect of Replacement of Soybean Meal with Fermented Rice Bran as Protein Source on Growth Response, Feeding Utilization, Body Composition, Intestinal Microbiota and an Economic Evaluation of the Grey mullet, Mugil cephalus

Genomic and transcriptomic analyses of the elite rice variety Huizhan provide insight into disease resistance and heat tolerance

The indica rice variety Huizhan shows elite traits of disease resistance and heat tolerance. However, the underlying genetic basis of these traits is not fully understood due to limited genomic resources. Here, we used Nanopore long-read and next-generation sequencing technologies to generate a chromosome-scale genome assembly of Huizhan. Comparative genomics analysis uncovered a large chromosomal inversion and expanded gene families that are associated with plant growth, development and stress responses. Functional rice blast resistance genes, including Pi2, Pib and Ptr, and bacterial blight resistance gene Xa27, contribute to disease resistance of Huizhan. Furthermore, integrated genomics and transcriptomics analyses showed that OsHIRP1, OsbZIP60, the SOD gene family, and various transcription factors are involved in heat tolerance of Huizhan. The high-quality genome assembly and comparative genomics results presented in this study facilitate the use of Huizhan as an elite parental line in developing rice varieties adapted to disease pressure and climate challenges.

Evaluating growth and flowering response of tulip varieties under Tirumala#39;s agro-climatic conditions

Evaluating growth and flowering response of tulip varieties under Tirumala#39;s agro-climatic conditions

Effects of Salicylic Acid, Melatonin, and Mycorrhizal Fungi on the Growth and Physiological Responses of Wheat Under Varying Water Irrigation Stress Levels

Effects of Salicylic Acid, Melatonin, and Mycorrhizal Fungi on the Growth and Physiological Responses of Wheat Under Varying Water Irrigation Stress Levels

The Role of Tissue Factor In Signaling Pathways of Pathological Conditions and Angiogenesis.

Tissue factor (TF) is an integral transmembrane protein associated with the extrinsic coagulation pathway. TF gene expression is regulated in response to inflammatory cytokines, bacterial lipopolysaccharides, and mechanical injuries. TF activity may be affected by phosphorylation of its cytoplasmic domain and alternative splicing. TF acts as the primary initiator of physiological hemostasis, which prevents local bleeding at the injury site. However, aberrant expression of TF, accompanied by the severity of diseases and infections under various pathological conditions, triggers multiple signaling pathways that support thrombosis, angiogenesis, inflammation, and metastasis. Protease-activated receptors (PARs) are central in the downstream signaling pathways of TF. In this study, we have reviewed the TF signaling pathways in different pathological conditions, such as wound injury, asthma, cardiovascular diseases (CVDs), viral infections, cancer and pathological angiogenesis. Angiogenic activities of TF are critical in the repair of wound injuries and aggressive behavior of tumors, which are mainly performed by the actions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 (HIF1-α). Pro-inflammatory effects of TF have been reported in asthma, CVDs and viral infections, including COVID-19, which result in tissue hypertrophy, inflammation, and thrombosis. TF-FVII induces angiogenesis via clotting-dependent and -independent mechanisms. Clottingdependent angiogenesis is induced via the generation of thrombin and cross-linked fibrin network, which facilitate vessel infiltration and also act as a reservoir for endothelial cells (ECs) growth factors. Expression of TF in tumor cells and ECs triggers clotting-independent angiogenesis through induction of VEGF, urokinase-type plasminogen activator (uPAR), early growth response 1 (EGR1), IL8, and cysteine-rich angiogenic inducer 61 (Cyr61).

378 Growth responses and antioxidant status of finishing pigs fed low-energy, low-phosphorus, low-calcium and low-trace mineral diets added with exogenous enzymes

378 Growth responses and antioxidant status of finishing pigs fed low-energy, low-phosphorus, low-calcium and low-trace mineral diets added with exogenous enzymes

Daily and weekly urine variations in bacterial growth susceptibility in postmenopausal women with no history of urinary tract infection: a pilot study

ObjectivesTo evaluate bacterial growth in the bladder as a major virulence factor during urinary tract infections (UTIs), we assessed the variability of uropathogenic bacterial growth in urine samples over multiple timepoints from three postmenopausal women with no history of UTI. MethodsFollowing IRB approval, postmenopausal women who never had a UTI provided mid-stream urine samples three times daily (8 a.m., 12 p.m. and 4 p.m.), twice a week over two weeks, as well as dietary intake logs. Each sample was studied for pH and bacterial growth using three uropathogenic Escherichia coli (E.coli) strains (LRPF007, KE40, UTI89), one uropathogenic Enterococcus faecalis strain (HRH40), and one non-pathogenic E. coli strain (W3110). ResultsSimilar mean growth yields were observed for the uropathogenic strains (mean ∆OD600 = 0.1 – 0.13) with high variability (standard deviation (SD) = 0.13 – 0.28) and lower mean growth for the non-pathogenic strain (mean ∆OD600 = 0.05). Urine from each individual at different collection times never had the same bacterial growth potential for any bacterial strain. There were significant associations between urine pH decrease with higher fat (p=0.017), vitamin D (p=0.02), magnesium (p=0.049), fluid (p=0.013), and ash (p=0.01) intake. ConclusionsMajor variations were observed in the bacterial growth response in the urine of the same individuals. These variations imply a requirement for a normalization procedure, e.g., growth in synthetic urine, for analysis of bacterial growth in urine. There were significant associations between decreased urine pH and several nutrients found in foods that are classified as highly acidic.

Pyrazolines inhibiting the activity of the early growth response-1 DNA-binding domain

To identify compounds inhibiting the activity of the Early Growth Response (EGR)-1 DNA-binding domain, thirty-seven pyrazolines were prepared and their EGR-1 DNA-binding activities were measured. Pharmacophores were derived based on quantitative structure–activity relationship calculations. As compound 2, 1-(5-(4-methoxyphenyl)-4,5-dihydro-1H-pyrazol-3-yl)naphthalen-2-ol, showed the best inhibitory effects against the activity of the EGR-1 DNA-binding domain, the binding mode between compound 2 and EGR-1 was elucidated using in silico docking. The pharmacophores were matched to the binding modes. Electrophoretic mobility shift assays confirmed that compound 2 dose-dependently inhibited TNFα-induced EGR-1-DNA complex formation in HaCaT cells. Reverse transcription-polymerase chain reaction demonstrated that compound 2 effectively reduced the mRNA expression of EGR-1-regulated inflammatory genes, including thymic stromal lymphopoietin (TSLP), interleukin (IL)-1β, IL-6, and IL-31, in TNFα-stimulated HaCaT cells. Therefore, compound 2 could be developed as an agent that inhibits the activity of the EGR-1 DNA-binding domain.