Reduction In Growth Research Articles

Construction, molecular characterization, and safety assessment of purB mutant of Salmonella Gallinarum

This study involves the development and molecular characterization of the isogenic markerless knockout mutant SG ΔpurB, a genetically engineered live attenuated strain aimed at controlling Salmonella Gallinarum (SG) infection in poultry. The mutant was generated by deleting the purB gene using λ-Red recombination technology, impairing adenylosuccinate lyase, necessary for purine biosynthesis. An 1,180 bp deletion was engineered within the purB gene, leaving a residual 298 bp genomic scar resulting in a purine auxotrophic mutant. Phenotypically, SG ΔpurB showed a 66.5% reduction in growth in LB broth compared to the wild-type strain and failed to grow in minimal media without adenosine. Growth was restored to near wild-type levels with 0.3 mM adenosine supplementation, demonstrating the strain’s conditional attenuation. In vivo pathogenicity assessments revealed that oral inoculation of SG ΔpurB into 3-day-old chickens at a dose of 2 × 108 CFU resulted in zero mortality, compared to an 80% mortality rate in chickens challenged with the wild-type strain. The SG ΔpurB strain exhibited significantly reduced clinical signs and lesion scores, with clinical sign scores dropping from 2.5/3 with the wild-type to 0.4/3 with the ΔpurB mutant, and lesion scores decreasing from 2.9/3 to 0.3/3. Additionally, the mutant was efficiently cleared from liver and spleen tissues by 14 days post-inoculation, unlike the wild-type strain, which persisted until the experiment’s end on day 21. The SG ΔpurB mutant shows potential as a safe alternative for preventing fowl typhoid, highlighting the promise of targeted genetic attenuation in developing effective vaccines for poultry diseases.

Investigating the Antagonistic Effects of Novel Combination Fungicides against Fusarium oxysporum f. sp. ricini under In-vitro Conditions

Aim: The study aimed to assess the efficacy of nine novel fungicide combinations against Fusarium oxysporum f. sp. ricini, the causal agent of wilt disease in castor. Methodology: The efficacy of fungicides was evaluated using the poisoned food technique under in-vitro conditions. Radial mycelial growth of the pathogen was measured across various concentrations (100 to 1000 ppm) of the fungicide combinations to determine their impact on mycelial inhibition. Results: Results indicated a substantial reduction in radial mycelial growth with increased fungicide concentration. Among the tested fungicides, Tebuconazole + Trifloxystrobin and Prochloraz + Tebuconazole recorded complete mycelial inhibition (100 %) at all the tested concentrations, demonstrating significant fungicidal activity followed by Hexaconazole + Captan with a mean mycelial inhibition of 85.2 %, indicating strong potential for disease management. In contrast, the least mean mycelial inhibition (24.7 %) was observed with Thiophanate methyl + Pyraclostrobin. Conclusion: These findings underscored the importance of selecting effective fungicide combinations to manage F. oxysporum f. sp. ricini, thereby enhancing crop protection and agricultural sustainability.

Strategies for feeding tilapia in smallholder aquaculture systems: a study of aquaculture feed access and availability in two districts of Northern Province, Zambia

ABSTRACT Aquaculture contributes to agricultural development, economic growth, and poverty reduction initiatives in Zambia. In northern and rural regions of the country, development requires improvements to the management of local and farm-generated inputs. Fish farmers have limited market access and purchasing power, which restricts access to commercial inputs, such as formulated feeds and chemical fertilizers. Instead, farmers adopt integrated-agriculture-aquaculture (IAA) practices, whereby on-farm resources supply essential inputs. Research is limited regarding local systems of resource management, and this hinders development of innovations for improving efficiency. This mixed-methods study explored local patterns of resource utilization. Smallholders (n = 63) participated in surveys and interviews regarding resource management. A series of quantitative analyses, including descriptive statistics, principal component analyses (PCA), and multinomial logistic regression (logit) models, identified relationships between these resources, household livelihood, and system productivity. Qualitative analyses explored farmer perceptions regarding seasonal resource availability. Results suggested that livestock manure, homemade feeds comprising maize and soybean, garden residues, and kitchen wastes were the most frequently selected inputs. Logit models of feeding intensity found that income, proximity to Luwingu Town, experience in aquaculture, and access to extension services were key household resources. High-intensity feeding strategies incorporated commercial feeds, chemical fertilizers, as well as home-made feeds containing a wide diversity of both high- and low-quality ingredients. Low-intensity feeding strategies incorporated only farm waste and termites. Potential innovations that may improve system performance in resource poor contexts are considered and recommendations for additional research are offered.

Two decades of population change in Saudi Arabia: Exploring effective redistribution strategies

Census 2022 of Saudi Arabia was released recently, with 12 years of intercensal interval. Although it appeared provisional having no reports similar to the 2010 census, efforts to analyze, interpret, disseminate, and discuss were essential for building structures and systems at par with demographic trends and patterns. An analysis was carried out with this census data compared to 2004 and 2010 to track population change—demographic pace, trends, and patterns—over the two decades. Data from all three censuses were analyzed with conventional demographic techniques. A reduction in growth was observed with a declining percentage of the childhood population but with an expanding percentage of the adults (working age) indicating a demographic dividend resulted, mostly, from fertility decline. An aging trend established by the previous censuses was lost, recently: the constriction of the pyramid of 2010 was changed to a different shape. Not only the percentage distribution trends were uneven but also the age-based indices. Thus, these trends revealed a demographic difference to an extent, that demands standardized reports, uniform procedures for the data collection and compilation, and geographic distribution equations. The increasing concentration in urban centers of major administrative areas—Al-Riyadh, Makkah Al-Mokarramah, and the Eastern Region demand redistribution policies. Self-contained townships appear as a strategic option in population redistribution, guaranteeing quality standards and lifestyle.

Renewable energy, carbon footprints, natural resources depletion and economic growth in Africa

Purpose This study aims to examine the interplay between renewable energy consumption, carbon footprints, natural resources depletion and economic growth. Design/methodology/approach It engaged 45 African countries using the generalized method of moments (GMM) approach. Data from the World Development Indicators for the period 2000–2023 are used to analyse the relationships among these variables. Findings The result indicates a positive and significant effect of greenhouse gas emissions on economic growth in all regions of Africa, except for Southern Africa. Regarding the depletion of natural resources, the authors observe a dominant negative effect on economic growth. Thus, an increase in the depletion of natural resources contributes to the reduction of economic growth in most regions of Africa, notably West Africa, East Africa and sub-Saharan Africa as a whole. Moreover, the depletion of natural resources can also have negative social impacts, such as conflicts over access to remaining resources, which can indirectly influence economic stability and growth. Originality/value This study contributes to the existing literature by providing empirical evidence of the positive effects of renewable energy consumption on carbon footprints, natural resource depletion and economic growth. By quantifying these relationships, the study offers valuable insights into the potential of renewable energy to address pressing environmental and economic challenges.

Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila

The Mediator complex plays a pivotal role in facilitating RNA polymerase II-dependent transcription in eukaryotes. Within this complex, the CDK8 kinase module (CKM), comprising CDK8, Cyclin C (CycC), Med12, and Med13, serves as a dissociable subcomplex that modulates the activity of the small Mediator complex. Genetic studies in Drosophila have revealed distinct phenotypes associated with mutations in CKM subunits, but the underlying mechanisms have remained unclear. Using Drosophila as a model, we generated transgenic strains to individually or simultaneously deplete the four CKM subunits in all possible combinations, uncovering unique phenotypes in the eyes and wings. Depletion of CDK8-CycC enhanced E2F1 target gene expression and promoted cell-cycle progression, whereas Med12-Med13 depletion had no significant impact on these processes. Instead, depleting Med12-Med13 altered the expression of ribosomal protein genes and fibrillarin, reduced nascent protein synthesis, indicating a severe reduction in ribosome biogenesis and cellular growth compared to the loss of CDK8-CycC. These findings reveal distinct in vivo roles for CKM subunits, with Med12-Med13 disruption having a more pronounced effect on ribosome biogenesis and protein synthesis than CDK8-CycC loss.

Evaluation of Microplastic Toxicity in Drinking Water Using Different Test Systems

Microplastic pollution poses a significant threat to environmental and human health. This study investigated the toxicological and genotoxic effects of various microplastic types (polystyrene (PS), polyethylene terephthalate (PET), polypropylene (PP), and polyethylene (PE)) on plant and animal models. Aqueous extracts of microplastics in different size fractions (0.175 mm, 0.3 mm, 1 mm, 2 mm, and 3 mm) were evaluated for their impact on barley seed germination and cell division. Results indicated that smaller microplastic fractions exhibited higher toxicity, particularly for PP and PE. Significant reductions in germination rates and root growth were observed, along with increased chromosomal aberrations in barley cells. Furthermore, the migration of formaldehyde, a known toxicant, from microplastics exceeded permissible limits. These findings highlight the potential risks associated with microplastic pollution, particularly in drinking water sources. Future research should focus on the long-term health impacts of microplastic exposure, including carcinogenic potential, and explore the synergistic effects with other pollutants. Stricter regulations on microplastic pollution and advancements in water treatment technologies are urgently needed to mitigate these risks.

Cecal Microbial Diversity and Metabolome Reveal a Reduction in Growth Due to Oxidative Stress Caused by a Low-Energy Diet in Donkeys

Dietary energy level plays an important role in animal growth and development. The present study investigated the effect of dietary energy on the growth performance, antioxidative state, and nutrient digestion of meat donkeys. It simultaneously explored the probable reason for cecal microbiota and metabolome. Twelve meat donkeys (male) aged 1 year with a similar weight (150 ± 25 kg) were divided into two treatment groups: low-energy group (E1) and high-energy group (E2). The experiment was divided into a 10-day pre-trial period and a 135-day trial period. Donkeys in the trial periods were fed diets with digestible energy values (in dry matter) of 12.08 and 13.38 MJ/kg (pre-fattening, 1–45 d), 13.01 and 14.07 MJ/kg (mid-fattening, 46–90 d), and 13.54 and 14.93 MJ/kg (late-fattening, 91–135 d). The results show that E1 decreases body weight, average daily gain (ADG), and the digestibility of crude protein, ether extract, neutral detergent fiber, and acid detergent fiber (p < 0.05), but increases cecal acetate and butyrate concentrations, non-esterified fatty acids (NEFAs) in serum, and the ratio of dry matter intake to ADG(F/G). E1 diminished the activities of catalase and glutathione peroxidase, while it increased the content of interleukin, tumor necrosis factor-alpha, and reactive oxygen species (ROS) (p < 0.05). Cecal microbiome showed that the abundance of Firmicutes and Actinobacteria in E1 was significantly lower than in E2 (p = 0.029, p = 0.002), whereas Bacteroidetes was higher (p = 0.005). E1 increased the genera Ruminococcaceae-UCG-004, Acinetobacter, and Rikenellaceae_RC9_gut_group. Meanwhile, cecal metabolome showed that formyl-5-hydroxykynurenamine, chorismate, 3-sulfinoalanine, and 3-isopropylmalate were upregulated in E1, while brassinolide was downregulated. The altered metabolites were mainly enriched in metabolic pathways related to energy metabolism and metabolism to mitigate oxidative stress in the meat donkeys, such as tryptophan metabolism, brassinosteroid biosynthesis metabolism, etc. In conclusion, low-energy diets resulted in a negative energy balance in meat donkeys, resulting in more nutrients being oxidized to provide energy, inducing oxidative stress, and thereby leading to decreasing growth. The reduction in meat donkey growth from low-energy diets was related to changes in cecum microbiota and metabolites.

Evaluating the Influence of Nutrient-Rich Substrates on the Growth and Waste Reduction Efficiency of Black Soldier Fly Larvae

Background: The black soldier fly (Hermetia illucens) has emerged as a promising tool in sustainable waste management, owing to its larvae’s ability to efficiently convert organic waste into valuable biomass. Objective: This study investigates the impact of various substrate compositions on the growth, waste reduction efficiency, and bioconversion rate of black soldier fly (BSF) larvae (Hermetia illucens). The aim is to optimize feeding strategies to enhance the effectiveness of BSF larvae in sustainable waste management and protein production. Methods: A controlled experiment was conducted over a 20-day period, using four different substrate types: 100% sludge, 75% sludge + 25% chicken feed, 25% sludge + 75% chicken feed, and 100% chicken feed. Each treatment had three replicates with 100 larvae each. Larval growth metrics, including weight and width, were recorded bi-daily. The waste reduction efficiency and bioconversion rate were calculated based on the remaining substrate weight and larval biomass, respectively. Elemental analysis was performed to determine the impact of substrate type on the accumulation of various elements in the larvae. Results: Significant differences were observed in larval growth, waste reduction efficiency, and bioconversion rates across the different substrates. The 100% chicken feed substrate led to the highest larval growth (M = 0.0881 g/day, SD = 0.0042) and bioconversion rate (M = 7.52%, SD = 0.34), while the 100% sludge substrate achieved the highest waste reduction rate (M = 86.2%, SD = 2.15). ANOVA tests indicated that substrate composition significantly affected these outcomes (p < 0.05). Elemental analysis showed substantial variations in the concentrations of calcium, cadmium, and nickel among the substrates, with the 100% sludge substrate having the highest nickel accumulation (M = 0.2763 ppm, SD = 0.023), significantly different from the other treatments (p < 0.001). Conclusions: The results demonstrate that substrate composition is crucial for optimizing BSF larvae growth and waste reduction efficiency. Nutrient-rich substrates, such as chicken feed, significantly enhance bioconversion rates and larval biomass production, although careful consideration of elemental accumulation, especially heavy metals, is essential for safe application in animal feed.

Sympathetic Neurons Promote Small Cell Lung Cancer Through the Beta-2 Adrenergic Receptor.

The vagal nerve is linked to tumorigenesis in multiple tissues including small cell lung cancer (SCLC). However, the role of sympathetic neuron in SCLC development remains unknown. Here, we observed a significant reduction in tumor growth following chemical denervation of local sympathetic nerves in a mouse model of SCLC. Further study identified that β2-adrenergic receptor (ADRB2) on cancer cells mediated the crosstalk with nerve fibers. Genetic deletion or pharmacological inhibition of ADRB2 led to reduced tumor growth and improved survival. Moreover, blocking ADRB2 also reduced the growth of human SCLC organoids and xenografts. Further studies revealed that ADRB2 promoted cancer cell expansion through activating Protein Kinase A (PKA) signaling. Consistently, inhibition of PKA also reduced the growth of SCLC cells. These findings offer the initial insight into the role played by sympathetic neurons in the development of SCLC and may open a new therapeutic avenue to treat this deadly malignancy.

Are Biobased Microfibers Less Harmful than Conventional Plastic Microfibers: Evidence from Earthworms.

Biobased plastics are sometimes promoted as "environmentally friendly" compared to their conventional petrochemical-based counterparts, but their ecotoxicity is only partially understood. Biobased fibers are widely used in clothing and wet wipes and can accumulate in soils through the application of biosolid fertilizers. This study examined the lethal thresholds and sublethal toxicity of chemically characterized, additive-free, biobased (viscose and lyocell) compared to petrochemical-based (polyester) fibers on the key ecosystem engineer, Esenia fetida. Viscose and lyocell had LC20 values of 14.00 and 22.66 mg·L-1, respectively, and no observed effect concentrations (NOEC) of 0-2.8 mg·L-1 (72 h, OECD TG207 filter paper method), while for polyester these were LC20 15.6-31.3 mg·L-1 and NOEC 0-15.6 mg·L-1. Following 28 days of exposure to soils (OECD TG222) contaminated with environmentally relevant concentrations (100 mg kg-1), viscose significantly reduced the mass of progeny compared to polyester. Earthworms exposed to lyocell had a marginal growth reduction (-18%; compared to -11% to -13% in other treatments) linked to increased bioturbation activity. The biobased fibers examined here have greater acute toxicity at high concentrations and broadly similar sublethal effects on E. fetida compared to polyester. Our study highlights the importance of detailed testing before advocating specific materials as plastic alternatives/substitutes to conventional plastics.

Effects of SNP, MgSO4, and MgO-NPs foliar application on Spinacia oleracea L. growth and physio-biochemical responses under cadmium stress

The effects of foliar application of sodium nitroprusside (SNP), magnesium sulfate (MgSO4) and magnesium oxide nanoparticles (MgO-NPs) on the growth, physiology, and gas exchange parameters of two varieties of spinach (Spinacia oleracea L.) under cadmium (Cd) stress were examined. The experiment was arranged in a completely randomized design with 72 pots. Two varieties of S. oleracea (Desi Palak & Lahori Palak) were used. Two concentrations of Cd (0 µM and 150 µM) in the form of cadmium chloride (CdCl2) were used. Two levels of SNP (0 ppm and 100 ppm) and two levels for each form of Mg i.e. MgSO4 and MgO-NPs (0 and 200 ppm) were foliar sprayed on plants in control and Cd stress. Both varieties behaved similarly under Cd stress and caused reductions in growth, physiology, gas exchange, water content parameters and inorganic ion uptake. However, the biochemical parameters like relative membrane permeability (RMP), malondialdehyde (MDA), and hydrogen peroxide (H2O2) contents were increased. However, all foliar spray treatments increased growth, physiological and gas exchange parameters, water content and inorganic ion uptake. However, this reduced the MDA, RMP, and H2O2 contents. Desi Palak showed the more positive results under foliar application of MgO-NPs. However, Lahori palak showed more positive results under the SNP + MgO-NP treatment. It is concluded that foliar application of SNP, MgSO4 and MgO-NPs could be an innovative approach to alleviated the heavy metals (Cd) toxicity in crop plants.

Inhibitory effects of N-trans-cinnamoyltyramine on growth of invasive weeds and weedy rice.

N-trans-cinnamoyltyramine (NTCT) has been identified from an allelopathic Vietnamese rice accession OM 5930. This study employed bioassays to analyze NTCT's effects on shoot and root growth of multiple test and weed species. NTCT demonstrated potent inhibitory effects on cress, lettuce, canola, palmer amaranth, timothy, barnyardgrass, red sprangletop, and weedy rice, with increasing concentrations leading to substantial reductions in growth in all species. Linear regression analysis of dose response curves revealed ED50 values for NTCT, providing critical insights into the concentration required for 50% growth inhibition in each species. They revealed high sensitivity of the test species cress and lettuce, intermediate sensitivities of barnyardgrass, red sprangletop, timothy, and amaranth, and comparatively lower sensitivity of two weedy rice accessions. The findings underscore NTCT's efficacy in suppressing the growth of a wide range of weeds, including both grasses and broadleaf species. As such, NTCT may hold promise as a tool for sustainable weed management, particularly in addressing herbicide-resistant weeds in diverse ecological settings.

Protection of understory vegetation by deer exclosure fences prevent the reduction of beech growth due to soil erosion

Overbrowsing by ungulates has degraded understory vegetation in worldwide forests. Understory degradation causes soil erosion and environmental degradation, causing mature tree decline. Although ungulate exclosure fences are installed to protect vegetation, their effectiveness in preventing tree decline associated with soil degradation remains unclear. We aimed to fill this gap on slopes of Mt. Shiraga, Japan, where sika deer have overbrowsed for >20 years. Tree-ring records in 1960–2022 for eight beeches per each of inside and outside fences were analyzed to compare stem basal area increment (BAI, cm−2 yr−1) and trend change timings for BAI (i.e., break-point). Soil properties, exposed root height (ERH) as cumulative soil erosion index, and intrinsic water use efficiency (iWUE) as water limitation proxy were also compared. Compared with areas inside fences, areas outside fences had no understory vegetation, higher ERH, and higher soil hardness and moisture. BAI outside fences turned their growth trend from positive to negative since 2004, but not inside fences. Break-point of BAI outside fences matched the understory degradation period in the field. The long-term BAI trend (i.e., regression slope of BAI) after the break-point was negatively related to ERH but not to soil properties (e.g., soil hardness and moisture). Furthermore, BAI outside fences was negatively related to iWUE after the break-point, whereas BAI inside fences was not. These results indicate that the maintenance of understory by fencing prevents overbrowsing-associated mature tree decline through mitigating root exposure and related water limitation, despite the potential of water competition with understory.

Fostamatinib Inhibits the Proliferation of Ovarian Cancer Cells Through Apoptosis Induction.

Ovarian cancer remains a significant challenge due to its high mortality rate and poor prognosis, especially in advanced stages. Despite treatment advancements, issues with resistance and recurrence persist, highlighting the urgent need for new and effective therapies. This study aimed to evaluate fostamatinib, an oral spleen tyrosine kinase inhibitor initially developed for autoimmune diseases, as a potential treatment for ovarian cancer. The effects of fostamatinib on ovarian cancer cell lines were assessed using WST-1 assays for cell proliferation. Apoptosis was evaluated through TUNEL assays, DNA fragmentation analysis, and flow cytometry. Western blot analysis was used to detect cleavage of apoptotic proteins, including caspase-3 and PARP, and flow cytometry analyzed cell cycle changes. Fostamatinib treatment resulted in a dose- and time-dependent reduction in ovarian cancer cell growth and induced apoptosis, as indicated by increased TUNEL-positive cells, DNA fragmentation, and rises in both early and late apoptosis. Western blot analysis showed increased cleavage of apoptotic proteins, including caspase-3 and PARP. Flow cytometry also demonstrated an increase in the sub-G1 phase of the cell cycle, further supporting apoptosis induction. Fostamatinib, by inhibiting cell proliferation and inducing apoptosis, shows promise as a repurposed therapeutic agent for ovarian cancer, potentially offering a new approach to improve patient outcomes.

Guavira Fruit Pomace Promotes Immunomodulation and Reduction of Tumor Growth in Walker 256 Tumor-Bearing Rats

Background: Guavira fruit is widely used for juice extraction purposes, and this process generates large amounts of pomace (waste). Guavira pomace was dried and milled to produce guavira pomace flour (GPF), which is rich in antioxidants and dietary fibers (polysaccharides). These compounds are known for their immunomodulatory and antitumor effects. Objective: To investigate whether GPF intake promotes immunomodulation and reduces Walker 256 tumor growth in rats. Methods: GPF was provided to Wistar rats in two different models: 1) 15-day Model, according to which, Walker 256 tumor-bearing rats received GPF (63 mg/200 g b.w./day) simultaneously to tumor growth for 15 days; 2) 45-day Model, according to which, tumor-bearing rats received GPF for 30 days before tumor cell implantation, as well as during tumor growth - it totaled 45 days. After animals were euthanized, tumors were collected and weighed, and tumor cells were isolated for proliferation capacity determination ex vivo. Enzymatic/colorimetric methods were used to determine resident peritoneal macrophages’ functionality, whereas blood T and B lymphocytes were assayed for proliferation capacity, ex vivo, under stimuli. Results: The 15-day Model did not show tumor mass or cell proliferation reduction in the treated group. GPF stimulated macrophage response in tumor-bearing and non-tumor-bearing rats. However, there was a substantial reduction in tumor mass and tumor cell proliferation under the 45-day Model. Macrophage and lymphocyte response decreased; it suggested that GPF can directly act in the tumor. Conclusion: Based on these findings, GPF has immunomodulatory and antitumor actions, and ingestion time plays a key role in them.

Nanomedicine potential of Cymbopogon citratus Linn. –Biogenic synthesized silver nanoparticles: A study on antimicrobial and anticancer efficacy

Green synthesis methods for making nanoparticles using plant extracts have gained considerable attention in recent years, particularly because of their potential applications in nanomedicine. Plant biosynthesized nanoparticles have shown noteworthy biomedical uses in comparison. The silver nanoparticle (AgNP) synthesized using extracts from the leaves and flowers of Cymbopogon citratus Linn was investigated for its antibacterial and anticancer properties. The biosynthesized AgNPs were characterized using UV–Vis, FTIR, DLS, EDX and SEM. The presence of alcohol, alkene, and phytochemicals in the AgNPs was confirmed using FTIR analysis. The investigation using SEM with EDX spectroscopy confirmed that the AgNPs were both pure and in the form of nanocrystals. Furthermore, AgNPs demonstrated significant antibacterial activity by efficiently suppressing bacterial growth. The biosynthesized AgNPs exhibited a concentration-dependent reduction in the lung cancer-A549 cell growth. The ethanolic extracts of C. citratus flower-synthesized AgNPs contain a wide range of phytochemical constituents. These components have been shown to effectively modulate antibacterial and anticancer activities, superior tothe effects of leaf extracts synthesized AgNPs. Consequently, additional research is required to investigate the potential biomedical applications of these biosynthesized AgNPs.

The evolution of inequality in Mozambique 1996/97–2019/20

ABSTRACT After decades of war, Mozambique experienced sustained economic growth and poverty reduction from the mid-1990s. However, these positive dynamics started to revert from 2015. Meanwhile, inequality stagnated in the period 1996/97–2008/09, before markedly increasing afterwards. In this study, we analyse some of the most relevant indicators of inequality for Mozambique and their trends over the last 25 years. Using real per capita consumption as the main welfare aggregate, we look at various indicators of inequality, including the consumption distribution, percentiles and percentile ratios, growth incidence curves, Lorenz curves, and Gini indices. In addition, we discuss spatial inequality. Overall, we find that until 2014/15, consumption increased for the whole population, but it did so much more for richer households; conversely, in the last few years, consumption has reduced across the distribution, but the relative consumption-gap between better- and worse-off people has continued to increase.

Differentiated growth of the most widely planted conifer in response to extreme droughts across semi-arid regions in Northern China

Global warming is leading to more frequent and intense drought events, exerting unprecedented pressure on forest growth. Although post-drought recovery in plantation growth has been studied enormously, the variation of planted populations across the whole distribution range of a species is not well understood. In this study, the growth suitability of the most widely planted conifer species in dry lands of northern China, Mongolian Scots pine (Pinus sylvestris var. mongolica), was estimated, based on tree-ring data sampled in 289 plots of afforestation across most of its planting range. According to changes in the hydrothermal condition during the last forty years (1980–2018), the distribution range was divided into three parts: warming, warming-wetting, and non-significant trends (NT) regions. Two severe droughts in 2000 and 2007 were identified using the Standardized Precipitation Evapotranspiration Index (SPEI). Then, the resistance (Rt), recovery (Rc), and resilience (Rs) growth indices were calculated and compared among these three parts. The growth of planted Mongolian Scots pine has overall significantly increased from 1980 to 2018 (p < 0.001). The pine plantations in warming regions were sensitive to annual precipitation, while warming-wetting regions were more influenced by the growing season climate. Pines reverted to pre-drought growth levels within 3 years after the drought event in 2000 but failed in 2007, and populations in the warming region showed significantly lower post-drought recovery. Furthermore, high pre-drought growth levels were linked to low Rs in both drought events (p < 0.001). Post-drought precipitation enhanced the Rc of plantations, compensating for the growth reduction caused by droughts. Soil moisture during the drought events enhanced Rt and Rs. The Rt and Rs indices decreased significantly with the age of plantations (p < 0.05). Overall, our results revealed that pre-drought growth and post-drought precipitation were key factors influencing the resilience of planted Mongolian Scots pine. Besides, the plantations experiencing wetting trends revealed favorable drought resilience and stable radial growth, which indicated promising potential for planted Mongolian Scots pine in those areas. Controlling the age of plantations seems to be crucial for maintaining drought resilience in large-scale plantations and maximizing ecological benefits.

Thyroid and growth hormone endocrine disruption and mechanisms of homosalate and octisalate using wild-type, thrαa-/-, and dre-miR-499-/- zebrafish embryo/larvae

Homosalate (HS) and octisalate (OS), which are used in sunscreen for the purpose of blocking ultraviolet rays, are frequently detected in water environment. Although effects on estrogens and androgens have been reported, studies on thyroid and growth hormone endocrine disruption are limited. In the present study, larval mortality was compared in wild-type and two knockout fish (thyroid hormone receptor alpha a knockout (thrαa-/-) and dre-miR-499 knockout (dre-miR-499-/-)) after 96 h of exposure to HS and OS (0, 0.003, 0.03, 0.3, 3, 30 and 300 µg/L). To investigate the mechanisms of thyroid and growth hormone endocrine disruption, we measured the levels of triiodothyronine (T3), thyroxine (T4), thyroid stimulating hormone (TSH), growth hormone (GH), and insulin-like growth factor-1 (IGF-1), and the regulation of representative genes related to the hypothalamus-pituitary-thyroid (HPT) and GH/IGF axis in wild-type zebrafish exposed to target chemicals. The significantly lower larval survival rate of thrαa-/- and dre-miR-499-/- fish exposed to 300 μg/L of HS and OS suggest that thyroid hormone receptors and dre-miR-499 play a crucial role in the toxic effects of HS and OS. The finding of a significant increase in T3 and T4 in zebrafish larvae exposed to HS and OS supports a significant decrease in the crh gene. The reduction of GH and IGF-1 in fish exposed to HS and OS is well supported by the regulation of genes involved in the GH/IGF axis. Our observations suggest that exposure to HS and OS affects not only thyroid hormone receptors and their associated miRNAs, but also the feedback routes of HPT and GH/IGF axes, ultimately leading to growth reduction.