Freezing fraction and collision coefficient are the key parameters in the ice accretion model, which should be calculated to predict the icing trend for transmission lines. Here a mathematical relation between freezing fraction and collision coefficient was derived using the simplified head balance model of transmission lines icing. Combined with the empirical formula of the liquid water content in air, a calculation method of the freezing fraction and collision coefficient was proposed using the ice surface temperature of transmission lines and the icing mass growth rate. The method to measure the icing mass growth rate based on online monitoring data and icing experiments was proposed. The icing experiments were carried out in the low-temperature ice chamber with the same parameters as the online monitoring environmental parameters. The values of the freezing fraction and collision coefficient were calculated by using the online monitoring data and icing experiments data respectively, the calculation results were compared. The maximum relative differences between the calculation results from the online monitoring data and the icing experiments data do not exceed 18%, which proves the validity of the calculation method of the freezing fraction and collision coefficient in ice accretion model of transmission lines.